Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle

Detalhes bibliográficos
Autor(a) principal: Salgado, Miriane Carneiro Machado
Data de Publicação: 2015
Outros Autores: Marchin, Adriana Mathias Pereira da Silva, Tera, Tábata de Mello, Rocha, Rosilene Fernandes da, Marchini, Leonardo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian journal of oral sciences (Online)
Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839
Resumo: Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.
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spelling Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyleMandibleMandibular condyleEthanolOvariectomyOdontologiaEstrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.Universidade Estadual de Campinas2015-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839Brazilian Journal of Oral Sciences; v. 14 n. 1 (2015): Jan./Mar.; 16-22Brazilian Journal of Oral Sciences; Vol. 14 No. 1 (2015): Jan./Mar.; 16-221677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPporhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839/8371Copyright (c) 2015 Miriane Carneiro Machado Salgado, Adriana Mathias Pereira da Silva Marchin, Tábata de Mello Tera, Rosilene Fernandes da Rocha, Leonardo Marchinihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSalgado, Miriane Carneiro MachadoMarchin, Adriana Mathias Pereira da SilvaTera, Tábata de MelloRocha, Rosilene Fernandes daMarchini, Leonardo2023-10-02T12:36:50Zoai:ojs.periodicos.sbu.unicamp.br:article/8640839Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2023-10-02T12:36:50Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
title Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
spellingShingle Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
Salgado, Miriane Carneiro Machado
Mandible
Mandibular condyle
Ethanol
Ovariectomy
Odontologia
title_short Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
title_full Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
title_fullStr Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
title_full_unstemmed Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
title_sort Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
author Salgado, Miriane Carneiro Machado
author_facet Salgado, Miriane Carneiro Machado
Marchin, Adriana Mathias Pereira da Silva
Tera, Tábata de Mello
Rocha, Rosilene Fernandes da
Marchini, Leonardo
author_role author
author2 Marchin, Adriana Mathias Pereira da Silva
Tera, Tábata de Mello
Rocha, Rosilene Fernandes da
Marchini, Leonardo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Salgado, Miriane Carneiro Machado
Marchin, Adriana Mathias Pereira da Silva
Tera, Tábata de Mello
Rocha, Rosilene Fernandes da
Marchini, Leonardo
dc.subject.por.fl_str_mv Mandible
Mandibular condyle
Ethanol
Ovariectomy
Odontologia
topic Mandible
Mandibular condyle
Ethanol
Ovariectomy
Odontologia
description Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839/8371
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Estadual de Campinas
publisher.none.fl_str_mv Universidade Estadual de Campinas
dc.source.none.fl_str_mv Brazilian Journal of Oral Sciences; v. 14 n. 1 (2015): Jan./Mar.; 16-22
Brazilian Journal of Oral Sciences; Vol. 14 No. 1 (2015): Jan./Mar.; 16-22
1677-3225
reponame:Brazilian journal of oral sciences (Online)
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Brazilian journal of oral sciences (Online)
collection Brazilian journal of oral sciences (Online)
repository.name.fl_str_mv Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv brjorals@fop.unicamp.br||brjorals@fop.unicamp.br
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