Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839 |
Resumo: | Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups. |
id |
UNICAMP-8_c1f8dddd98c9ca4bd84b1c2f2c054dcf |
---|---|
oai_identifier_str |
oai:ojs.periodicos.sbu.unicamp.br:article/8640839 |
network_acronym_str |
UNICAMP-8 |
network_name_str |
Brazilian journal of oral sciences (Online) |
repository_id_str |
|
spelling |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyleMandibleMandibular condyleEthanolOvariectomyOdontologiaEstrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups.Universidade Estadual de Campinas2015-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839Brazilian Journal of Oral Sciences; v. 14 n. 1 (2015): Jan./Mar.; 16-22Brazilian Journal of Oral Sciences; Vol. 14 No. 1 (2015): Jan./Mar.; 16-221677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPporhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839/8371Copyright (c) 2015 Miriane Carneiro Machado Salgado, Adriana Mathias Pereira da Silva Marchin, Tábata de Mello Tera, Rosilene Fernandes da Rocha, Leonardo Marchinihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSalgado, Miriane Carneiro MachadoMarchin, Adriana Mathias Pereira da SilvaTera, Tábata de MelloRocha, Rosilene Fernandes daMarchini, Leonardo2023-10-02T12:36:50Zoai:ojs.periodicos.sbu.unicamp.br:article/8640839Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2023-10-02T12:36:50Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
spellingShingle |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle Salgado, Miriane Carneiro Machado Mandible Mandibular condyle Ethanol Ovariectomy Odontologia |
title_short |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_full |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_fullStr |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_full_unstemmed |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
title_sort |
Effects of estrogen deficiency combined with chronic alcohol consumption on rat mandibular condyle |
author |
Salgado, Miriane Carneiro Machado |
author_facet |
Salgado, Miriane Carneiro Machado Marchin, Adriana Mathias Pereira da Silva Tera, Tábata de Mello Rocha, Rosilene Fernandes da Marchini, Leonardo |
author_role |
author |
author2 |
Marchin, Adriana Mathias Pereira da Silva Tera, Tábata de Mello Rocha, Rosilene Fernandes da Marchini, Leonardo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Salgado, Miriane Carneiro Machado Marchin, Adriana Mathias Pereira da Silva Tera, Tábata de Mello Rocha, Rosilene Fernandes da Marchini, Leonardo |
dc.subject.por.fl_str_mv |
Mandible Mandibular condyle Ethanol Ovariectomy Odontologia |
topic |
Mandible Mandibular condyle Ethanol Ovariectomy Odontologia |
description |
Estrogen deficiency and chronic alcohol consumption may have a synergistic and deleterious effect on bone tissue. Aim: To investigate the effects of estrogen deficiency associated with chronic alcohol consumption on the mandibular condyle in rats. Methods: Fifty-four female rats were first divided equally into two groups: ovariectomized (Ovx) and simulated ovariectomy (Sham). One month after the surgeries, these groups were equally sub-divided according to their dietary treatment: G1: Sham/ad-libitum diet; G2: Sham/alcohol; G3: Sham/isocaloric; G4: Ovx/ad-libitum diet; G5: Ovx/alcohol, G6: Ovx/isocaloric. Eight weeks after starting the diets, all animals were anesthetized and sacrificed. The condyles were analyzed histologically, histomorphometrically, and immunohistochemically using the antibodies for bone sialoprotein (BSP), osteocalcin (OCC) and receptor activator of nuclear factor kappa-B ligand (RANKL). Results: Histological analysis of the mandibular condyles showed that Ovx and Sham groups presented almost the same characteristics. The histomorphometric analysis showed that there was a statistically significant difference only between Ovx/isocaloric and Ovx/ad-libitum groups (p=0.049). No difference was observed in the intensity of BSP, OCC, and RANKL antibody staining between the Ovx/alcohol and the other groups. Conclusions: It may be concluded that there was no histomorphometric, histological, or RANKL, BSP, and OCC staining differences between the Ovx/alcohol group and other experimental groups. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640839/8371 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 14 n. 1 (2015): Jan./Mar.; 16-22 Brazilian Journal of Oral Sciences; Vol. 14 No. 1 (2015): Jan./Mar.; 16-22 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1800216397705379840 |