Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA

Detalhes bibliográficos
Autor(a) principal: Ferrari, Débora Oro
Data de Publicação: 2018
Outros Autores: Lund, Rafael Guerra, Zanella, Luana, Roman Júnior, Walter Antônio, Rodrigues-Junior, Sinval Adalberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian journal of oral sciences (Online)
Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8654158
Resumo: Denture use may aggravate the occurrence of oral infections, considering it enhances microbial adherence. Aim: This study assessed the reduction of microbial loads of Candida albicans, Staphylococcus aureus, and Klebsiella oxytoca by disinfecting the polymethylmethacrylate (PMMA) of complete dentures with hydroalcoholic extract of Salvia officinalis. Additionally, the effect of such extract on the properties of PMMA was examined. Methods: Microorganisms were isolated from saliva samples collected from complete denture wearers. The hydroalcoholic extract of S. officinalis was produced according to the Brazilian Pharmacopoeia 5. The PMMA specimens (n=188) were immersed in microbial inoculum and incubated at 37°C for 16 hours per day. Then, they were subjected to a disinfection protocol for 30 days. The specimens were divided into five treatment groups: sterile saline solution (0.85%; control), 0.2% chlorhexidine digluconate, and hydroalcoholic extract of S. officinalis (0.2%, 0.8%, and 1.16%). Microorganism adherence to the PMMA surface was also assessed, as well as surface roughness (Ra in µm) and color stability of the PMMA (mean ΔE). Changes in microbial load and surface roughness after the disinfection protocol were verified with paired t-test. Substances at day 10, adherence, and color stability were compared by the Kruskall-Wallis and Mann-Whitney tests, and one-way ANOVA was used to compare substances at the beginning and end of the experiment (α=0.05). Results: The 1.16% S. officinalis extract significantly reduced the microbial load of all the microorganisms after 30 days of disinfection (p<0.05). The microbial load of K. oxytoca was also reduced at lower concentrations of the S. officinalis extract (0.2% and 0.8%) (p<0.02). Antimicrobial and anti-adherent effects against microorganisms isolated from the oral cavity were observed. There was no significant change in surface roughness (p>0.05) and color stability was significantly higher in the control group (p<0.0001). Conclusions: The hydroalcoholic extract of S. officinalis may be used as a disinfectant solution for dentures.
id UNICAMP-8_d0d5605802ed2cefb9ccc49b55c26572
oai_identifier_str oai:ojs.periodicos.sbu.unicamp.br:article/8654158
network_acronym_str UNICAMP-8
network_name_str Brazilian journal of oral sciences (Online)
repository_id_str
spelling Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMAColony countmicrobial. Denture cleansers. Plant extracts. Polymethyl methacrylate.Denture use may aggravate the occurrence of oral infections, considering it enhances microbial adherence. Aim: This study assessed the reduction of microbial loads of Candida albicans, Staphylococcus aureus, and Klebsiella oxytoca by disinfecting the polymethylmethacrylate (PMMA) of complete dentures with hydroalcoholic extract of Salvia officinalis. Additionally, the effect of such extract on the properties of PMMA was examined. Methods: Microorganisms were isolated from saliva samples collected from complete denture wearers. The hydroalcoholic extract of S. officinalis was produced according to the Brazilian Pharmacopoeia 5. The PMMA specimens (n=188) were immersed in microbial inoculum and incubated at 37°C for 16 hours per day. Then, they were subjected to a disinfection protocol for 30 days. The specimens were divided into five treatment groups: sterile saline solution (0.85%; control), 0.2% chlorhexidine digluconate, and hydroalcoholic extract of S. officinalis (0.2%, 0.8%, and 1.16%). Microorganism adherence to the PMMA surface was also assessed, as well as surface roughness (Ra in µm) and color stability of the PMMA (mean ΔE). Changes in microbial load and surface roughness after the disinfection protocol were verified with paired t-test. Substances at day 10, adherence, and color stability were compared by the Kruskall-Wallis and Mann-Whitney tests, and one-way ANOVA was used to compare substances at the beginning and end of the experiment (α=0.05). Results: The 1.16% S. officinalis extract significantly reduced the microbial load of all the microorganisms after 30 days of disinfection (p<0.05). The microbial load of K. oxytoca was also reduced at lower concentrations of the S. officinalis extract (0.2% and 0.8%) (p<0.02). Antimicrobial and anti-adherent effects against microorganisms isolated from the oral cavity were observed. There was no significant change in surface roughness (p>0.05) and color stability was significantly higher in the control group (p<0.0001). Conclusions: The hydroalcoholic extract of S. officinalis may be used as a disinfectant solution for dentures.Universidade Estadual de Campinas2018-12-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionOriginal articleapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/865415810.20396/bjos.v17i0.8654158Brazilian Journal of Oral Sciences; v. 17 (2018): Continuous Publication; e18901Brazilian Journal of Oral Sciences; Vol. 17 (2018): Continuous Publication; e189011677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8654158/19124Ferrari, Débora OroLund, Rafael GuerraZanella, LuanaRoman Júnior, Walter AntônioRodrigues-Junior, Sinval Adalbertoinfo:eu-repo/semantics/openAccess2020-12-22T01:34:08Zoai:ojs.periodicos.sbu.unicamp.br:article/8654158Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2020-12-22T01:34:08Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
title Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
spellingShingle Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
Ferrari, Débora Oro
Colony count
microbial. Denture cleansers. Plant extracts. Polymethyl methacrylate.
title_short Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
title_full Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
title_fullStr Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
title_full_unstemmed Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
title_sort Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA
author Ferrari, Débora Oro
author_facet Ferrari, Débora Oro
Lund, Rafael Guerra
Zanella, Luana
Roman Júnior, Walter Antônio
Rodrigues-Junior, Sinval Adalberto
author_role author
author2 Lund, Rafael Guerra
Zanella, Luana
Roman Júnior, Walter Antônio
Rodrigues-Junior, Sinval Adalberto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferrari, Débora Oro
Lund, Rafael Guerra
Zanella, Luana
Roman Júnior, Walter Antônio
Rodrigues-Junior, Sinval Adalberto
dc.subject.por.fl_str_mv Colony count
microbial. Denture cleansers. Plant extracts. Polymethyl methacrylate.
topic Colony count
microbial. Denture cleansers. Plant extracts. Polymethyl methacrylate.
description Denture use may aggravate the occurrence of oral infections, considering it enhances microbial adherence. Aim: This study assessed the reduction of microbial loads of Candida albicans, Staphylococcus aureus, and Klebsiella oxytoca by disinfecting the polymethylmethacrylate (PMMA) of complete dentures with hydroalcoholic extract of Salvia officinalis. Additionally, the effect of such extract on the properties of PMMA was examined. Methods: Microorganisms were isolated from saliva samples collected from complete denture wearers. The hydroalcoholic extract of S. officinalis was produced according to the Brazilian Pharmacopoeia 5. The PMMA specimens (n=188) were immersed in microbial inoculum and incubated at 37°C for 16 hours per day. Then, they were subjected to a disinfection protocol for 30 days. The specimens were divided into five treatment groups: sterile saline solution (0.85%; control), 0.2% chlorhexidine digluconate, and hydroalcoholic extract of S. officinalis (0.2%, 0.8%, and 1.16%). Microorganism adherence to the PMMA surface was also assessed, as well as surface roughness (Ra in µm) and color stability of the PMMA (mean ΔE). Changes in microbial load and surface roughness after the disinfection protocol were verified with paired t-test. Substances at day 10, adherence, and color stability were compared by the Kruskall-Wallis and Mann-Whitney tests, and one-way ANOVA was used to compare substances at the beginning and end of the experiment (α=0.05). Results: The 1.16% S. officinalis extract significantly reduced the microbial load of all the microorganisms after 30 days of disinfection (p<0.05). The microbial load of K. oxytoca was also reduced at lower concentrations of the S. officinalis extract (0.2% and 0.8%) (p<0.02). Antimicrobial and anti-adherent effects against microorganisms isolated from the oral cavity were observed. There was no significant change in surface roughness (p>0.05) and color stability was significantly higher in the control group (p<0.0001). Conclusions: The hydroalcoholic extract of S. officinalis may be used as a disinfectant solution for dentures.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Original article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8654158
10.20396/bjos.v17i0.8654158
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8654158
identifier_str_mv 10.20396/bjos.v17i0.8654158
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8654158/19124
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Campinas
publisher.none.fl_str_mv Universidade Estadual de Campinas
dc.source.none.fl_str_mv Brazilian Journal of Oral Sciences; v. 17 (2018): Continuous Publication; e18901
Brazilian Journal of Oral Sciences; Vol. 17 (2018): Continuous Publication; e18901
1677-3225
reponame:Brazilian journal of oral sciences (Online)
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Brazilian journal of oral sciences (Online)
collection Brazilian journal of oral sciences (Online)
repository.name.fl_str_mv Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv brjorals@fop.unicamp.br||brjorals@fop.unicamp.br
_version_ 1800216402330648576