Cytotoxicity of intraoral orthodontic elastics

• Autor(a) principal: Santos, Rogério Lacerda
• Data de Publicação: 2016
• Outros Autores: Pithon, Matheus Melo, Oliveira, Márlio Vinícius, Mendes, Gabriella da Silva, Romanos, Maria Teresa Villela, Ruellas, Antônio Carlos de Oliveira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian journal of oral sciences (Online)
• Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8643024
• topic: Cytotoxicity. Elastics. Biocompatibility. Orthodontics; Odontologia

Aim: The present study compared in vitro four types of latex intraoral elastics (5/16 = 7.9 mm, mean load) regarding their possible cytotoxic effects. Material and Methods: The sample was allocated to four groups of 24 elastics: Group A (American Orthodontics), Group M (Morelli), Group T (TP Orthodontics), and Group U (Uniden). Cytotoxicity assays were performed using cell culture medium containing epithelioid-type cells (Hep-2 line) derived from human laryngeal carcinoma. Two methods for evaluating the cytotoxicity, the agar overlay test and “dye-uptake” test, were employed at two different moments (0 and 24 h). Data were compared by analysis of variance (ANOVA) and Tukey’s test (p<0.05). Results: The results showed statistically significant difference (p<0.05) between Groups T and M, T and U, A and M, and A and U, for dye-uptake test at 0 and 24 h. No cell lysis was found in Groups T and A for the agar overlay test, whereas 80% cell lysis was found in Groups M and U after 24 h according to the Stanford’s response index (RI). Conclusion: Both TP Orthodontics and American Orthodontics elastics evoked less cell lysis than Morelli and Uniden elastics