The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria

Detalhes bibliográficos
Autor(a) principal: Klug, Luiz Gustavo
Data de Publicação: 2017
Outros Autores: Storer, Carmen Lucia Mueller, Sebastiani, Aline Monise, Bsrbaresco, Bruna Lorena, Giovanini, Alan Fernando, Deliberador, Tatiana Miranda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian journal of oral sciences (Online)
Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8651053
Resumo: The proteins Wnts are considered a key regulator of the early development of the skeleton. Aim: The aim of this study was to evaluate the presence of the protein Wnt10b as a marker of bone repair in critical size defects surgically created in the calvaria of rabbits treated with fragmented autogenous adipose tissue graft. Methods: A total of 28 rabbits were divided into two groups: the Control group (C) and Adipose Tissue Graft group (ATG). A CSD measuring 15 mm in diameter was created in the calvaria of each animal. In rabbits of the C group, the defect was filled only with blood clot, and in ATG group, the defect was filled with fragmented adipose tissue graft. The two groups were divided into two subgroups (n = 7) for euthanasia 15 and 40 days after surgery. Histological and immunohistochemically analyses were performed to evaluate the neoformed bone and the presence/concentration of Wnt10b protein. The Kruskal-Wallis test was performed to compare the means and standard deviations of the number of Wnt10b + cells/mm2 in both groups in each postoperative period. It was assumed a significance level of 5%. Results: After 40 days, the mean concentration of the protein Wnt10b in ATG group was 26.26 (+-6.97) significant higher (p<0,001) than the mean in C group that was 305 (37.41). Conclusion: The protein Wnt10b would play a crucial role in the signaling of bone formation in bone defects treated with fragmented autogenous adipose tissue graft.
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spelling The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvariaAdipose Tissue. Bone regeneration. Rabbits. Wnt proteins.The proteins Wnts are considered a key regulator of the early development of the skeleton. Aim: The aim of this study was to evaluate the presence of the protein Wnt10b as a marker of bone repair in critical size defects surgically created in the calvaria of rabbits treated with fragmented autogenous adipose tissue graft. Methods: A total of 28 rabbits were divided into two groups: the Control group (C) and Adipose Tissue Graft group (ATG). A CSD measuring 15 mm in diameter was created in the calvaria of each animal. In rabbits of the C group, the defect was filled only with blood clot, and in ATG group, the defect was filled with fragmented adipose tissue graft. The two groups were divided into two subgroups (n = 7) for euthanasia 15 and 40 days after surgery. Histological and immunohistochemically analyses were performed to evaluate the neoformed bone and the presence/concentration of Wnt10b protein. The Kruskal-Wallis test was performed to compare the means and standard deviations of the number of Wnt10b + cells/mm2 in both groups in each postoperative period. It was assumed a significance level of 5%. Results: After 40 days, the mean concentration of the protein Wnt10b in ATG group was 26.26 (+-6.97) significant higher (p<0,001) than the mean in C group that was 305 (37.41). Conclusion: The protein Wnt10b would play a crucial role in the signaling of bone formation in bone defects treated with fragmented autogenous adipose tissue graft.Universidade Estadual de Campinas2017-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionCase studyapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/865105310.20396/bjos.v16i0.8651053Brazilian Journal of Oral Sciences; v. 16 (2017): Continuous Publication; 1-11Brazilian Journal of Oral Sciences; Vol. 16 (2017): Continuous Publication; 1-111677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8651053/18045Klug, Luiz GustavoStorer, Carmen Lucia MuellerSebastiani, Aline MoniseBsrbaresco, Bruna LorenaGiovanini, Alan FernandoDeliberador, Tatiana Mirandainfo:eu-repo/semantics/openAccess2020-12-22T01:34:18Zoai:ojs.periodicos.sbu.unicamp.br:article/8651053Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2020-12-22T01:34:18Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria
title The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria
spellingShingle The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria
Klug, Luiz Gustavo
Adipose Tissue. Bone regeneration. Rabbits. Wnt proteins.
title_short The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria
title_full The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria
title_fullStr The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria
title_full_unstemmed The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria
title_sort The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria
author Klug, Luiz Gustavo
author_facet Klug, Luiz Gustavo
Storer, Carmen Lucia Mueller
Sebastiani, Aline Monise
Bsrbaresco, Bruna Lorena
Giovanini, Alan Fernando
Deliberador, Tatiana Miranda
author_role author
author2 Storer, Carmen Lucia Mueller
Sebastiani, Aline Monise
Bsrbaresco, Bruna Lorena
Giovanini, Alan Fernando
Deliberador, Tatiana Miranda
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Klug, Luiz Gustavo
Storer, Carmen Lucia Mueller
Sebastiani, Aline Monise
Bsrbaresco, Bruna Lorena
Giovanini, Alan Fernando
Deliberador, Tatiana Miranda
dc.subject.por.fl_str_mv Adipose Tissue. Bone regeneration. Rabbits. Wnt proteins.
topic Adipose Tissue. Bone regeneration. Rabbits. Wnt proteins.
description The proteins Wnts are considered a key regulator of the early development of the skeleton. Aim: The aim of this study was to evaluate the presence of the protein Wnt10b as a marker of bone repair in critical size defects surgically created in the calvaria of rabbits treated with fragmented autogenous adipose tissue graft. Methods: A total of 28 rabbits were divided into two groups: the Control group (C) and Adipose Tissue Graft group (ATG). A CSD measuring 15 mm in diameter was created in the calvaria of each animal. In rabbits of the C group, the defect was filled only with blood clot, and in ATG group, the defect was filled with fragmented adipose tissue graft. The two groups were divided into two subgroups (n = 7) for euthanasia 15 and 40 days after surgery. Histological and immunohistochemically analyses were performed to evaluate the neoformed bone and the presence/concentration of Wnt10b protein. The Kruskal-Wallis test was performed to compare the means and standard deviations of the number of Wnt10b + cells/mm2 in both groups in each postoperative period. It was assumed a significance level of 5%. Results: After 40 days, the mean concentration of the protein Wnt10b in ATG group was 26.26 (+-6.97) significant higher (p<0,001) than the mean in C group that was 305 (37.41). Conclusion: The protein Wnt10b would play a crucial role in the signaling of bone formation in bone defects treated with fragmented autogenous adipose tissue graft.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Case study
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8651053
10.20396/bjos.v16i0.8651053
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8651053
identifier_str_mv 10.20396/bjos.v16i0.8651053
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8651053/18045
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Campinas
publisher.none.fl_str_mv Universidade Estadual de Campinas
dc.source.none.fl_str_mv Brazilian Journal of Oral Sciences; v. 16 (2017): Continuous Publication; 1-11
Brazilian Journal of Oral Sciences; Vol. 16 (2017): Continuous Publication; 1-11
1677-3225
reponame:Brazilian journal of oral sciences (Online)
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Brazilian journal of oral sciences (Online)
collection Brazilian journal of oral sciences (Online)
repository.name.fl_str_mv Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv brjorals@fop.unicamp.br||brjorals@fop.unicamp.br
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