MOLECULAR IDENTIFICATION OF FUSARIUM SPP
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Digital Unicesumar |
Texto Completo: | http://rdu.unicesumar.edu.br/handle/123456789/3676 |
Resumo: | Fusarium spp. have been widely studied because the mycotoxins they produce were a big threat to plants, animal and human health. This genus have species as known human opportunistic pathogens, mainly F. solani species complex (FSSC) and F. oxysporum species complex (FOSC). These species are able to cause a broad spectrum of infections in humans, ranging from skin infections such as keratitis and onychomycosis, up to disseminated infections. Classical methods are important for fungal identification, being the most used in the laboratory routine. However, molecular techniques are necessary for rapid diagnostic. In addition, for Fusarium spp. molecular approaches allow us to distinguish between species complexes. Seven clinical Fusarium isolates from patients with suspected onychomycosis were included to this study. Six samples from toenails (85.8%). By classical techniques, the clinical isolates were identified as Fusarium solani (71.5%) and Fusarium oxysporum (28.5%). The ITS region sequencing also identified these two Fusarium species. However, for two isolates was observed discrepant results between classical and molecular identification. Therefore, development of rapid and safe diagnostic methodology is essential for the correct management of patients. Molecular techniques are promising in this proposal. However, Fusarium species identification is still a major challenge. |
id |
UNICESU-1_2f61b445ce5e72726380a3c4452ac812 |
---|---|
oai_identifier_str |
oai:rdu.unicesumar.edu.br:123456789/3676 |
network_acronym_str |
UNICESU-1 |
network_name_str |
Repositório Digital Unicesumar |
repository_id_str |
|
spelling |
MOLECULAR IDENTIFICATION OF FUSARIUM SPPFusarium sppClinical isolatesPCRDNA sequencingFusarium spp. have been widely studied because the mycotoxins they produce were a big threat to plants, animal and human health. This genus have species as known human opportunistic pathogens, mainly F. solani species complex (FSSC) and F. oxysporum species complex (FOSC). These species are able to cause a broad spectrum of infections in humans, ranging from skin infections such as keratitis and onychomycosis, up to disseminated infections. Classical methods are important for fungal identification, being the most used in the laboratory routine. However, molecular techniques are necessary for rapid diagnostic. In addition, for Fusarium spp. molecular approaches allow us to distinguish between species complexes. Seven clinical Fusarium isolates from patients with suspected onychomycosis were included to this study. Six samples from toenails (85.8%). By classical techniques, the clinical isolates were identified as Fusarium solani (71.5%) and Fusarium oxysporum (28.5%). The ITS region sequencing also identified these two Fusarium species. However, for two isolates was observed discrepant results between classical and molecular identification. Therefore, development of rapid and safe diagnostic methodology is essential for the correct management of patients. Molecular techniques are promising in this proposal. However, Fusarium species identification is still a major challenge.UNIVERSIDADE CESUMARBrasilUNICESUMAR2019-12-30T18:21:07Z2019-12-30T18:21:07Z2019-10-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf978-85-459-1960-52594-4991http://rdu.unicesumar.edu.br/handle/123456789/3676porCONRADO, Pollyanna Cristina VincenziSAKITA, Karina MayumiARITA, Glaucia SayuriVOIDALESKI, Morgana FerreiraBONFIM-MENDONÇA, Patrícia de SouzaKIOSHIMA, Erika Sekiinfo:eu-repo/semantics/openAccessreponame:Repositório Digital Unicesumarinstname:Centro Universitário de Maringá (UNICESUMAR)instacron:UniCesumar2020-08-03T20:20:46Zoai:rdu.unicesumar.edu.br:123456789/3676Repositório InstitucionalPRIhttp://rdu.unicesumar.edu.br/oai/requestopendoar:2020-08-03T20:20:46Repositório Digital Unicesumar - Centro Universitário de Maringá (UNICESUMAR)false |
dc.title.none.fl_str_mv |
MOLECULAR IDENTIFICATION OF FUSARIUM SPP |
title |
MOLECULAR IDENTIFICATION OF FUSARIUM SPP |
spellingShingle |
MOLECULAR IDENTIFICATION OF FUSARIUM SPP CONRADO, Pollyanna Cristina Vincenzi Fusarium spp Clinical isolates PCR DNA sequencing |
title_short |
MOLECULAR IDENTIFICATION OF FUSARIUM SPP |
title_full |
MOLECULAR IDENTIFICATION OF FUSARIUM SPP |
title_fullStr |
MOLECULAR IDENTIFICATION OF FUSARIUM SPP |
title_full_unstemmed |
MOLECULAR IDENTIFICATION OF FUSARIUM SPP |
title_sort |
MOLECULAR IDENTIFICATION OF FUSARIUM SPP |
author |
CONRADO, Pollyanna Cristina Vincenzi |
author_facet |
CONRADO, Pollyanna Cristina Vincenzi SAKITA, Karina Mayumi ARITA, Glaucia Sayuri VOIDALESKI, Morgana Ferreira BONFIM-MENDONÇA, Patrícia de Souza KIOSHIMA, Erika Seki |
author_role |
author |
author2 |
SAKITA, Karina Mayumi ARITA, Glaucia Sayuri VOIDALESKI, Morgana Ferreira BONFIM-MENDONÇA, Patrícia de Souza KIOSHIMA, Erika Seki |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
CONRADO, Pollyanna Cristina Vincenzi SAKITA, Karina Mayumi ARITA, Glaucia Sayuri VOIDALESKI, Morgana Ferreira BONFIM-MENDONÇA, Patrícia de Souza KIOSHIMA, Erika Seki |
dc.subject.por.fl_str_mv |
Fusarium spp Clinical isolates PCR DNA sequencing |
topic |
Fusarium spp Clinical isolates PCR DNA sequencing |
description |
Fusarium spp. have been widely studied because the mycotoxins they produce were a big threat to plants, animal and human health. This genus have species as known human opportunistic pathogens, mainly F. solani species complex (FSSC) and F. oxysporum species complex (FOSC). These species are able to cause a broad spectrum of infections in humans, ranging from skin infections such as keratitis and onychomycosis, up to disseminated infections. Classical methods are important for fungal identification, being the most used in the laboratory routine. However, molecular techniques are necessary for rapid diagnostic. In addition, for Fusarium spp. molecular approaches allow us to distinguish between species complexes. Seven clinical Fusarium isolates from patients with suspected onychomycosis were included to this study. Six samples from toenails (85.8%). By classical techniques, the clinical isolates were identified as Fusarium solani (71.5%) and Fusarium oxysporum (28.5%). The ITS region sequencing also identified these two Fusarium species. However, for two isolates was observed discrepant results between classical and molecular identification. Therefore, development of rapid and safe diagnostic methodology is essential for the correct management of patients. Molecular techniques are promising in this proposal. However, Fusarium species identification is still a major challenge. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-30T18:21:07Z 2019-12-30T18:21:07Z 2019-10-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
978-85-459-1960-5 2594-4991 http://rdu.unicesumar.edu.br/handle/123456789/3676 |
identifier_str_mv |
978-85-459-1960-5 2594-4991 |
url |
http://rdu.unicesumar.edu.br/handle/123456789/3676 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UNIVERSIDADE CESUMAR Brasil UNICESUMAR |
publisher.none.fl_str_mv |
UNIVERSIDADE CESUMAR Brasil UNICESUMAR |
dc.source.none.fl_str_mv |
reponame:Repositório Digital Unicesumar instname:Centro Universitário de Maringá (UNICESUMAR) instacron:UniCesumar |
instname_str |
Centro Universitário de Maringá (UNICESUMAR) |
instacron_str |
UniCesumar |
institution |
UniCesumar |
reponame_str |
Repositório Digital Unicesumar |
collection |
Repositório Digital Unicesumar |
repository.name.fl_str_mv |
Repositório Digital Unicesumar - Centro Universitário de Maringá (UNICESUMAR) |
repository.mail.fl_str_mv |
|
_version_ |
1813098696508702720 |