Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Universitas. Ciências da saúde (Online) |
Texto Completo: | https://www.publicacoes.uniceub.br/cienciasaude/article/view/2564 |
Resumo: | Early diagnosis of congenital toxoplasmosis is highly important, since it can make the treatment possible and reduces sequela for the infant. Serological diagnosis alone cannot be accurate when it does not identify the IgA, IgM or IgG antibodies of low avidity, which do not cross the placent barrier. Therefore, parasitemic identification is important to be carried out by demonstrating the parasite in the peritoneal exsudatum of mice inoculated with suspected biological material, however such method is little sensitive and too much time-consuming. This research study aims at optimizing mice inoculation through serological screening, and encephalic histopathology, in order to identify whether there was contamination. Out of 138 fetuses and/or newborn samples taken from pregnant women positive for active toxoplasmosis inoculated intraperitoneally in mice, only 5 showed positive through parasite demonstration in the peritoneal exsudatum. Histopathology showed the agent in 45 cases and in 67 the presence of anti-toxoplasmas antibodies in the mice bloodstream by using indirect immunofluorescence technique. Mice serology and encephalic histopathology in addition to reducing the amount of time necessary for the outcomes from 120 to 60 days increased the positivity of 3.6% to 50.4% and 33.8%, respectively. |
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Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564Congenital Toxoplasmosis, Parasitological Diagnosis, Mice Inoculation, Experimental SerologyEarly diagnosis of congenital toxoplasmosis is highly important, since it can make the treatment possible and reduces sequela for the infant. Serological diagnosis alone cannot be accurate when it does not identify the IgA, IgM or IgG antibodies of low avidity, which do not cross the placent barrier. Therefore, parasitemic identification is important to be carried out by demonstrating the parasite in the peritoneal exsudatum of mice inoculated with suspected biological material, however such method is little sensitive and too much time-consuming. This research study aims at optimizing mice inoculation through serological screening, and encephalic histopathology, in order to identify whether there was contamination. Out of 138 fetuses and/or newborn samples taken from pregnant women positive for active toxoplasmosis inoculated intraperitoneally in mice, only 5 showed positive through parasite demonstration in the peritoneal exsudatum. Histopathology showed the agent in 45 cases and in 67 the presence of anti-toxoplasmas antibodies in the mice bloodstream by using indirect immunofluorescence technique. Mice serology and encephalic histopathology in addition to reducing the amount of time necessary for the outcomes from 120 to 60 days increased the positivity of 3.6% to 50.4% and 33.8%, respectively.UniCEUBCapesSilva, Marcos GontijoAvelino, Mariza MartinsAmaral, Waldemar NavesCastro, Ana Maria2013-12-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.publicacoes.uniceub.br/cienciasaude/article/view/256410.5102/ucs.v11i2.2564Universitas: Ciências da Saúde; v. 11, n. 2 (2013); 75-811981-97301678-5398reponame:Universitas. Ciências da saúde (Online)instname:Centro de Ensino de Brasília (UNICEUB)instacron:UNICEUBporhttps://www.publicacoes.uniceub.br/cienciasaude/article/view/2564/2179info:eu-repo/semantics/openAccess2014-04-01T19:32:47Zoai:oai.uniceub.emnuvens.com.br:article/2564Revistahttps://www.publicacoes.uniceub.br/cienciasaude/indexPRIhttps://publicacoesacademicas.uniceub.br/cienciasaude/oai||universitas.saude@uniceub.br1981-97301678-5398opendoar:2014-04-01T19:32:47Universitas. Ciências da saúde (Online) - Centro de Ensino de Brasília (UNICEUB)false |
dc.title.none.fl_str_mv |
Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564 |
title |
Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564 |
spellingShingle |
Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564 Silva, Marcos Gontijo Congenital Toxoplasmosis, Parasitological Diagnosis, Mice Inoculation, Experimental Serology |
title_short |
Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564 |
title_full |
Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564 |
title_fullStr |
Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564 |
title_full_unstemmed |
Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564 |
title_sort |
Optimizing the parasitological diagnosis of congenital toxoplasmosis - doi: 10.5102/ucs.v11i2.2564 |
author |
Silva, Marcos Gontijo |
author_facet |
Silva, Marcos Gontijo Avelino, Mariza Martins Amaral, Waldemar Naves Castro, Ana Maria |
author_role |
author |
author2 |
Avelino, Mariza Martins Amaral, Waldemar Naves Castro, Ana Maria |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Capes |
dc.contributor.author.fl_str_mv |
Silva, Marcos Gontijo Avelino, Mariza Martins Amaral, Waldemar Naves Castro, Ana Maria |
dc.subject.por.fl_str_mv |
Congenital Toxoplasmosis, Parasitological Diagnosis, Mice Inoculation, Experimental Serology |
topic |
Congenital Toxoplasmosis, Parasitological Diagnosis, Mice Inoculation, Experimental Serology |
description |
Early diagnosis of congenital toxoplasmosis is highly important, since it can make the treatment possible and reduces sequela for the infant. Serological diagnosis alone cannot be accurate when it does not identify the IgA, IgM or IgG antibodies of low avidity, which do not cross the placent barrier. Therefore, parasitemic identification is important to be carried out by demonstrating the parasite in the peritoneal exsudatum of mice inoculated with suspected biological material, however such method is little sensitive and too much time-consuming. This research study aims at optimizing mice inoculation through serological screening, and encephalic histopathology, in order to identify whether there was contamination. Out of 138 fetuses and/or newborn samples taken from pregnant women positive for active toxoplasmosis inoculated intraperitoneally in mice, only 5 showed positive through parasite demonstration in the peritoneal exsudatum. Histopathology showed the agent in 45 cases and in 67 the presence of anti-toxoplasmas antibodies in the mice bloodstream by using indirect immunofluorescence technique. Mice serology and encephalic histopathology in addition to reducing the amount of time necessary for the outcomes from 120 to 60 days increased the positivity of 3.6% to 50.4% and 33.8%, respectively. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-12 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.publicacoes.uniceub.br/cienciasaude/article/view/2564 10.5102/ucs.v11i2.2564 |
url |
https://www.publicacoes.uniceub.br/cienciasaude/article/view/2564 |
identifier_str_mv |
10.5102/ucs.v11i2.2564 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.publicacoes.uniceub.br/cienciasaude/article/view/2564/2179 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UniCEUB |
publisher.none.fl_str_mv |
UniCEUB |
dc.source.none.fl_str_mv |
Universitas: Ciências da Saúde; v. 11, n. 2 (2013); 75-81 1981-9730 1678-5398 reponame:Universitas. Ciências da saúde (Online) instname:Centro de Ensino de Brasília (UNICEUB) instacron:UNICEUB |
instname_str |
Centro de Ensino de Brasília (UNICEUB) |
instacron_str |
UNICEUB |
institution |
UNICEUB |
reponame_str |
Universitas. Ciências da saúde (Online) |
collection |
Universitas. Ciências da saúde (Online) |
repository.name.fl_str_mv |
Universitas. Ciências da saúde (Online) - Centro de Ensino de Brasília (UNICEUB) |
repository.mail.fl_str_mv |
||universitas.saude@uniceub.br |
_version_ |
1800216837261099008 |