COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biota Amazônia |
Texto Completo: | https://periodicos.unifap.br/index.php/biota/article/view/1506 |
Resumo: | This study assessed the effectiveness of three anticoagulants in blood parameters of "cururu" stingrays Potamotrygon cf. histrix. Blood from ten individuals were collected and diluted with anticoagulants EDTA 5% and 10%, heparin 2500 and 5000 UI and sodium citrate 3.2%. A blood sample without anticoagulant was also evaluated. The blood of samples without anticoagulant and with sodium citrate 3.2% coagulated in 20% and 30% of the cases, respectively. Clumps of cells were observed during erythrocyte counting in 30% of samples with EDTA 10%, 40% of samples with heparin 2500 IU and 60% of samples with heparin 5000 IU. No alterations were observed on the erythrogram of "cururu" stingrays with different anticoagulants, the values of plasma glucose were similar in all groups and total protein levels were lower in the samples with EDTA 5% and 10%. The use of sodium citrate 3.2% is not recommended for blood sample conservation of Potamotrygon cf. histrix. stingrays, but anticoagulants did not affect the parameters analyzed in the determination of plasma glucose and erythrogram. Therefore, these results indicate that in order to blood coagulation the samples should be collected directly with any of these anticoagulants concentrations.Keywords: potamotrygonid; anticoagulants; blood; hematology. |
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COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE)COMPARAÇÃO DOS EFEITOS DE ANTICOAGULANTES USADOS NA COLHEITA SANGUÍNEA PARA A DETERMINAÇÃO DOS PARÂMETROS DO SANGUE EM ARRAIAS DE VIDA LIVRE DO GÊNERO Potamotrygon (ELASMOBRANCHII: POTAMOTRYGONIDAE)This study assessed the effectiveness of three anticoagulants in blood parameters of "cururu" stingrays Potamotrygon cf. histrix. Blood from ten individuals were collected and diluted with anticoagulants EDTA 5% and 10%, heparin 2500 and 5000 UI and sodium citrate 3.2%. A blood sample without anticoagulant was also evaluated. The blood of samples without anticoagulant and with sodium citrate 3.2% coagulated in 20% and 30% of the cases, respectively. Clumps of cells were observed during erythrocyte counting in 30% of samples with EDTA 10%, 40% of samples with heparin 2500 IU and 60% of samples with heparin 5000 IU. No alterations were observed on the erythrogram of "cururu" stingrays with different anticoagulants, the values of plasma glucose were similar in all groups and total protein levels were lower in the samples with EDTA 5% and 10%. The use of sodium citrate 3.2% is not recommended for blood sample conservation of Potamotrygon cf. histrix. stingrays, but anticoagulants did not affect the parameters analyzed in the determination of plasma glucose and erythrogram. Therefore, these results indicate that in order to blood coagulation the samples should be collected directly with any of these anticoagulants concentrations.Keywords: potamotrygonid; anticoagulants; blood; hematology.Este estudo avaliou a eficácia de três anticoagulantes em parâmetros sanguíneos de arraias cururu Potamotrygon sp. O sangue de dez animais foi coletado e diluído nos anticoagulantes: EDTA 5% e 10%, heparina 2.500 e 5.000 UI e citrato de sódio 3,2%, outra parte do sangue também foi analisada sem anticoagulante. O sangue de 20% e 30% das amostras de sangue que não continha anticoagulante e citrato de sódio (3,2%), respectivamente, coagularam. Grumos de células foram observados durante a contagem de eritrócitos em 30% das amostras com EDTA 10%, 40% das amostras com heparina 2.500 UI e 60% das amostras com heparina 5.000 UI. Não foram observadas alterações no eritrograma da arraia cururu com diferentes anticoagulantes, os valores da glicose plasmática foram semelhantes em todos os grupos, enquanto os níveis de proteínas totais foram menores nas amostras coletadas com EDTA 5% e 10%. Em arraias Potamotrygon sp., para a conservação de amostras de sangue não é recomendado o uso de citrato de sódio 3.2%, porém, para a determinação da glicose plasmática e do eritrograma os anticoagulantes usados não influenciaram os parâmetros analisados. Portanto, esses resultados indicam que para evitar qualquer sinal de coagulação do sangue deve ser coletadas amostras diretamente com qualquer uma dessas concentrações de anticoagulantes.Palavras-chave: potamotrigonídeos, anticoagulantes, sangue, hematologia.Universidade Federal do AmapáCNPq, CAPES, FAPEAM, IFAMOliveira, Adriano Teixeira deSantos, Marcio Quara de CarvalhoLemos, Jefferson Raphael GonzagaLima, Jackson PantojaRocha Aride, Paulo HenriqueTavares-Dias, MarcosMarcon, Jaydione Luiz2015-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigo Avaliado pelos Paresapplication/pdfhttps://periodicos.unifap.br/index.php/biota/article/view/150610.18561/2179-5746/biotaamazonia.v5n3p55-58Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota); v. 5, n. 3 (2015); 55-582179-5746reponame:Biota Amazôniainstname:Universidade Federal do Amapá (UNIFAP)instacron:UNIFAPenghttps://periodicos.unifap.br/index.php/biota/article/view/1506/v5n3p55-58.pdfBrasil; Amazônia, Amazonas; BarcelosDireitos autorais 2015 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota)http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2016-03-29T17:52:27Zoai:ojs.pkp.sfu.ca:article/1506Revistahttp://periodicos.unifap.br/index.php/biotaONGhttps://periodicos.unifap.br/index.php/biota/oai||juliosa@unifap.br2179-57462179-5746opendoar:2016-03-29T17:52:27Biota Amazônia - Universidade Federal do Amapá (UNIFAP)false |
dc.title.none.fl_str_mv |
COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE) COMPARAÇÃO DOS EFEITOS DE ANTICOAGULANTES USADOS NA COLHEITA SANGUÍNEA PARA A DETERMINAÇÃO DOS PARÂMETROS DO SANGUE EM ARRAIAS DE VIDA LIVRE DO GÊNERO Potamotrygon (ELASMOBRANCHII: POTAMOTRYGONIDAE) |
title |
COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE) |
spellingShingle |
COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE) Oliveira, Adriano Teixeira de |
title_short |
COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE) |
title_full |
COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE) |
title_fullStr |
COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE) |
title_full_unstemmed |
COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE) |
title_sort |
COMPARISON OF THE EFFECTS OF ANTICOAGULANTS USED IN BLOOD COLLECTION TO DETERMINE BLOOD PARAMETERS OF FREE-LIVING STINGRAYS FROM THE Potamotrygon GENUS (ELASMOBRANCHII: POTAMOTRYGONIDAE) |
author |
Oliveira, Adriano Teixeira de |
author_facet |
Oliveira, Adriano Teixeira de Santos, Marcio Quara de Carvalho Lemos, Jefferson Raphael Gonzaga Lima, Jackson Pantoja Rocha Aride, Paulo Henrique Tavares-Dias, Marcos Marcon, Jaydione Luiz |
author_role |
author |
author2 |
Santos, Marcio Quara de Carvalho Lemos, Jefferson Raphael Gonzaga Lima, Jackson Pantoja Rocha Aride, Paulo Henrique Tavares-Dias, Marcos Marcon, Jaydione Luiz |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
CNPq, CAPES, FAPEAM, IFAM |
dc.contributor.author.fl_str_mv |
Oliveira, Adriano Teixeira de Santos, Marcio Quara de Carvalho Lemos, Jefferson Raphael Gonzaga Lima, Jackson Pantoja Rocha Aride, Paulo Henrique Tavares-Dias, Marcos Marcon, Jaydione Luiz |
description |
This study assessed the effectiveness of three anticoagulants in blood parameters of "cururu" stingrays Potamotrygon cf. histrix. Blood from ten individuals were collected and diluted with anticoagulants EDTA 5% and 10%, heparin 2500 and 5000 UI and sodium citrate 3.2%. A blood sample without anticoagulant was also evaluated. The blood of samples without anticoagulant and with sodium citrate 3.2% coagulated in 20% and 30% of the cases, respectively. Clumps of cells were observed during erythrocyte counting in 30% of samples with EDTA 10%, 40% of samples with heparin 2500 IU and 60% of samples with heparin 5000 IU. No alterations were observed on the erythrogram of "cururu" stingrays with different anticoagulants, the values of plasma glucose were similar in all groups and total protein levels were lower in the samples with EDTA 5% and 10%. The use of sodium citrate 3.2% is not recommended for blood sample conservation of Potamotrygon cf. histrix. stingrays, but anticoagulants did not affect the parameters analyzed in the determination of plasma glucose and erythrogram. Therefore, these results indicate that in order to blood coagulation the samples should be collected directly with any of these anticoagulants concentrations.Keywords: potamotrygonid; anticoagulants; blood; hematology. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo Avaliado pelos Pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.unifap.br/index.php/biota/article/view/1506 10.18561/2179-5746/biotaamazonia.v5n3p55-58 |
url |
https://periodicos.unifap.br/index.php/biota/article/view/1506 |
identifier_str_mv |
10.18561/2179-5746/biotaamazonia.v5n3p55-58 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.unifap.br/index.php/biota/article/view/1506/v5n3p55-58.pdf |
dc.rights.driver.fl_str_mv |
Direitos autorais 2015 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota) http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2015 Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota) http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brasil; Amazônia, Amazonas; Barcelos |
dc.publisher.none.fl_str_mv |
Universidade Federal do Amapá |
publisher.none.fl_str_mv |
Universidade Federal do Amapá |
dc.source.none.fl_str_mv |
Biota Amazônia (Biote Amazonie, Biota Amazonia, Amazonian Biota); v. 5, n. 3 (2015); 55-58 2179-5746 reponame:Biota Amazônia instname:Universidade Federal do Amapá (UNIFAP) instacron:UNIFAP |
instname_str |
Universidade Federal do Amapá (UNIFAP) |
instacron_str |
UNIFAP |
institution |
UNIFAP |
reponame_str |
Biota Amazônia |
collection |
Biota Amazônia |
repository.name.fl_str_mv |
Biota Amazônia - Universidade Federal do Amapá (UNIFAP) |
repository.mail.fl_str_mv |
||juliosa@unifap.br |
_version_ |
1800218369005191168 |