CYTOGENETIC CHARACTERIZATION OF A Spatuloricaria (SILURIFORMES, LORICARIIDAE) SPECIES OF THE XINGU RIVER (PARÁ, AMAZON, BRAZIL)

• Autor(a) principal: Ferreira, Renata Oliveira
• Data de Publicação: 2014
• Outros Autores: Pereira, Adenilson Leão, Nagamachi, Cleusa Yoshiko, Pieczarka, Julio Cesar, de Sousa, Leandro Melo, Noronha, Renata Coelho Rodrigues
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Biota Amazônia
• Texto Completo: https://periodicos.unifap.br/index.php/biota/article/view/814

Genus Spatuloricaria (Loricariinae, Loricariidae) comprises 12 species which are not very precisely characterized, because morphological data are incipient and no karyotype information is available. In this work, was made the first cytogenetic characterization of a Spatuloricaria species of the Xingu River. Chromosomes were analyzed with conventional staining techniques, C banding, CMA3, DAPI, fluorescent in situ hybridization (FISH) with telomeric probes and rDNA18S. The results show that Spatuloricaria sp. has 2n=66 (6st/7sm/4m/16a) and a fundamental number (FN) of 92. The C-banding pattern revealed a distribution of heterochromatin in the centromeric and pericentromeric regions of the chromosomes and on the short arm of pair 15, coinciding with the DAPI labeling. FISH with rDNA 18S probes painted the terminal portion of chromosome pair eigth, corresponding to the CMA3 labeling, and a heteromorphism between the genomic blocks of this region was observed. The data obtained shall serve as cytotaxonomic markers for a better understanding of this group and its relationships within family Loricariidae, allowing inferences about the chromosomal evolution of the genus and its relations with other loricarids.Keywords: Loricariinae; cytotaxonomy; FISH; rDNA and telomeric probes.