Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12807 |
Resumo: | The objective of this study was to observe the effectiveness of the use of proteins associated with pregnancy (PAG’s) as a method of early pregnancy diagnosis in Girolando dairy cows. By using a colorimetric reaction test, recommended for use in cows from the 28th day after service, VISUAL-PAG from "IDEXX Laboratories". The sensitivity and specificity of the test were assessed at 25, 26, 27 and 28 days post-TAI, using 57 multiparous females submitted to four sequential collections of blood samples on the days described. The material was collected by venipuncture of the coccidian vessels in vacuum tubes without anti-coagulant, processed and stored at -20ºC for later analysis. At 32 days of TAI all females were subjected to the diagnosis of pregnancy by ultrasonography (US), the Gold Test of the experiment. The results were obtained through a concordance study, which revealed 100% sensitivity and 94.7% specificity for days 25, 26, 27 and 28 post-TAI. In view of these results, it can be considered that the VISUAL-PAG kit can be used as an early and safe method of early pregnancy diagnosis in Girolando dairy cows at 25 days after artificial insemination. |
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Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cowsUso de proteínas asociadas con el embarazo (PAG's - Bovine Pregnancy Test Idexx®) como método de diagnóstico precoz del embarazo en vacas lecheras gyrolandoUtilização das proteínas associadas à gestação (PAG’s - Bovine Pregnancy Test Idexx®) como método de diagnóstico precoce de gestação em vacas leiteiras da raça girolandoBovinocultura leiteiraDiagnósticoGirolandoPag´s.Dairy cattleDiagnosticsGirolandoPag´s.Bovinocultura lecheraDiagnósticoGirolandoPag´s.The objective of this study was to observe the effectiveness of the use of proteins associated with pregnancy (PAG’s) as a method of early pregnancy diagnosis in Girolando dairy cows. By using a colorimetric reaction test, recommended for use in cows from the 28th day after service, VISUAL-PAG from "IDEXX Laboratories". The sensitivity and specificity of the test were assessed at 25, 26, 27 and 28 days post-TAI, using 57 multiparous females submitted to four sequential collections of blood samples on the days described. The material was collected by venipuncture of the coccidian vessels in vacuum tubes without anti-coagulant, processed and stored at -20ºC for later analysis. At 32 days of TAI all females were subjected to the diagnosis of pregnancy by ultrasonography (US), the Gold Test of the experiment. The results were obtained through a concordance study, which revealed 100% sensitivity and 94.7% specificity for days 25, 26, 27 and 28 post-TAI. In view of these results, it can be considered that the VISUAL-PAG kit can be used as an early and safe method of early pregnancy diagnosis in Girolando dairy cows at 25 days after artificial insemination.El objetivo de este estudio fue observar la efectividad del uso de proteínas asociadas con la gestación (PAG) como método de diagnóstico precoz del prenhez en vacas lecheras Girolando. Através de una prueba de reacción colorimétrica, recomendada para su uso en vacas a partir del 28° día posterior al servicio, el VISUAL-PAG de "IDEXX Laboratories". La sensibilidad y especificidad de la prueba se verificaron a los 25, 26, 27 y 28 días después de la Inseminación Artificial de Tiempo Fijo (IATF), utilizando 57 hembras multíparas, enviadas a cuatro colecciones secuenciales de muestras de sangre en los días descritos. El material se recolectó mediante venopunción de los vasos coccígeos en tubos de vacío sin anticoagulante, se procesó y almacenó a -20ºC para su posterior análisis. A los 32 días después de IATF, todas las hembras fueron sometidas a un diagnóstico de embarazo por ultrasonido (US), la prueba estándar del experimento. Los resultados se obtuvieron a través de un estudio de acuerdo, donde reveló un 100% de sensibilidad y un 94,7% de especificidad para los días 25, 26, 27 y 28 posteriores a la IATF. En vista de estos resultados, se puede considerar que el kit VISUAL-PAG puede usarse como un método de diagnóstico precoz y seguro del prenhez en vacas lecheras Girolando a los 25 días después de la inseminación artificial.Objetivou-se com este estudo observar a eficácia da utilização das Proteínas Associadas à Gestação (PAG´s) como método de diagnóstico precoce de gestação em vacas leiteiras da raça Girolando. Por meio de um teste de reação colorimétrica, recomendado para uso em vacas a partir do 28º dia pós-serviço, o VISUAL-PAG do “IDEXX Laboratories”. Verificou-se a sensibilidade e especificidade do teste aos 25, 26, 27 e 28 dias pós-Inseminação Artificial em Tempo Fixo (IATF), utilizando-se 57 fêmeas multíparas, submetidas a quatro coletas sequenciais de amostras de sangue nos dias descritos. O material foi coletado através de venopunção dos vasos coccígenos em tubos de vácuo sem anticoagulante, processado e armazenado a -20ºC para posterior análise. Aos 32 dias da IATF todas as fêmeas foram submetidas ao diagnóstico de gestação por ultrassonografia (US), teste padrão do experimento. Os resultados foram obtidos através de um estudo de concordância, onde revelou sensibilidade de 100% e especificidade de 94,7% iguais para os dias 25, 26, 27 e 28 pós-IATF. Diante destes resultados pode-se considerar que o kit VISUAL-PAG pode ser utilizado como um método de diagnóstico precoce e seguro de gestação em vacas leiteiras da raça Girolando aos 25 dias pós-inseminação artificial.Research, Society and Development2021-02-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1280710.33448/rsd-v10i2.12807Research, Society and Development; Vol. 10 No. 2; e41410212807Research, Society and Development; Vol. 10 Núm. 2; e41410212807Research, Society and Development; v. 10 n. 2; e414102128072525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/12807/11422Copyright (c) 2021 Edivânia Maria de Freitas; Breno Barros de Santana; Carlos Rhuan Rocha Malta; José Augusto Tenório de Sousa Bernardo; Emanuel Felipe de Oliveira Filho; Gustavo Ferrer Carneiro https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFreitas, Edivânia Maria de Santana, Breno Barros de Malta, Carlos Rhuan Rocha Bernardo, José Augusto Tenório de Sousa Oliveira Filho, Emanuel Felipe de Carneiro , Gustavo Ferrer 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12807Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:17.495114Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows Uso de proteínas asociadas con el embarazo (PAG's - Bovine Pregnancy Test Idexx®) como método de diagnóstico precoz del embarazo en vacas lecheras gyrolando Utilização das proteínas associadas à gestação (PAG’s - Bovine Pregnancy Test Idexx®) como método de diagnóstico precoce de gestação em vacas leiteiras da raça girolando |
| title |
Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows |
| spellingShingle |
Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows Freitas, Edivânia Maria de Bovinocultura leiteira Diagnóstico Girolando Pag´s. Dairy cattle Diagnostics Girolando Pag´s. Bovinocultura lechera Diagnóstico Girolando Pag´s. |
| title_short |
Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows |
| title_full |
Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows |
| title_fullStr |
Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows |
| title_full_unstemmed |
Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows |
| title_sort |
Use of proteins associated with pregnancy (PAG’s - Bovine Pregnancy Test Idexx®) as a method of early diagnosis of pregnancy in gyrolando dairy cows |
| author |
Freitas, Edivânia Maria de |
| author_facet |
Freitas, Edivânia Maria de Santana, Breno Barros de Malta, Carlos Rhuan Rocha Bernardo, José Augusto Tenório de Sousa Oliveira Filho, Emanuel Felipe de Carneiro , Gustavo Ferrer |
| author_role |
author |
| author2 |
Santana, Breno Barros de Malta, Carlos Rhuan Rocha Bernardo, José Augusto Tenório de Sousa Oliveira Filho, Emanuel Felipe de Carneiro , Gustavo Ferrer |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Freitas, Edivânia Maria de Santana, Breno Barros de Malta, Carlos Rhuan Rocha Bernardo, José Augusto Tenório de Sousa Oliveira Filho, Emanuel Felipe de Carneiro , Gustavo Ferrer |
| dc.subject.por.fl_str_mv |
Bovinocultura leiteira Diagnóstico Girolando Pag´s. Dairy cattle Diagnostics Girolando Pag´s. Bovinocultura lechera Diagnóstico Girolando Pag´s. |
| topic |
Bovinocultura leiteira Diagnóstico Girolando Pag´s. Dairy cattle Diagnostics Girolando Pag´s. Bovinocultura lechera Diagnóstico Girolando Pag´s. |
| description |
The objective of this study was to observe the effectiveness of the use of proteins associated with pregnancy (PAG’s) as a method of early pregnancy diagnosis in Girolando dairy cows. By using a colorimetric reaction test, recommended for use in cows from the 28th day after service, VISUAL-PAG from "IDEXX Laboratories". The sensitivity and specificity of the test were assessed at 25, 26, 27 and 28 days post-TAI, using 57 multiparous females submitted to four sequential collections of blood samples on the days described. The material was collected by venipuncture of the coccidian vessels in vacuum tubes without anti-coagulant, processed and stored at -20ºC for later analysis. At 32 days of TAI all females were subjected to the diagnosis of pregnancy by ultrasonography (US), the Gold Test of the experiment. The results were obtained through a concordance study, which revealed 100% sensitivity and 94.7% specificity for days 25, 26, 27 and 28 post-TAI. In view of these results, it can be considered that the VISUAL-PAG kit can be used as an early and safe method of early pregnancy diagnosis in Girolando dairy cows at 25 days after artificial insemination. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-02-21 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/12807 10.33448/rsd-v10i2.12807 |
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https://rsdjournal.org/index.php/rsd/article/view/12807 |
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10.33448/rsd-v10i2.12807 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
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https://rsdjournal.org/index.php/rsd/article/view/12807/11422 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 2; e41410212807 Research, Society and Development; Vol. 10 Núm. 2; e41410212807 Research, Society and Development; v. 10 n. 2; e41410212807 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052670986420224 |