Artificial insemination in quails through different methods of semen collection
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/2789 |
Resumo: | The objective of this study was to evaluate different methods of semen collection in quails and the possible viability of artificial insemination. Two collection methods were used: method 1: Abdominal massage in the male, and method 2: Abdominal massage in the female after male copulation. Were used 16 females and 13 males of the species Coturnix coturnix coturnix, aged 10 weeks. In the first method, the selected male was stimulated with the abdominal massage technique to release a foamy secretion through the glands for cloacal and his reproductive apparatus, which was collected in a disposable 1 ml insulin syringe, immediately and disposed in the vagina region of each pair of females in method 1. In the second method 2, the copulation of the selected male was performed in the mannequin females which received an abdominal massage for the removal of the male ejaculate. The eggs of each method were marked and incubated for 18 days. There was a fertility index of 0% in method 1, and 4.88% in method 2. After failure in fertility and feasibility rates, tests for sperm detection were sought in the male reproductive system through histological cuts. The conclusion was that the methods of artificial insemination were not efficient because did not exert the necessary pressure to enable sperm. Thus, the methods of semen collection and artificial insemination applied obtained undesirable indices and are not viable for reproductive management in quails. |
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Artificial insemination in quails through different methods of semen collectionInseminación artificial en codornices usando diferentes métodos de recolección de semenInseminação artificial em codornas através de diferentes métodos de coleta de sêmenAcasalamentoAparelho reprodutivoFertilidadeMassagemReprodução.apareamientoórganos reproductivosfertilidadmasajereproducción.MatingReproductive applianceFertilityMassageReproduction.The objective of this study was to evaluate different methods of semen collection in quails and the possible viability of artificial insemination. Two collection methods were used: method 1: Abdominal massage in the male, and method 2: Abdominal massage in the female after male copulation. Were used 16 females and 13 males of the species Coturnix coturnix coturnix, aged 10 weeks. In the first method, the selected male was stimulated with the abdominal massage technique to release a foamy secretion through the glands for cloacal and his reproductive apparatus, which was collected in a disposable 1 ml insulin syringe, immediately and disposed in the vagina region of each pair of females in method 1. In the second method 2, the copulation of the selected male was performed in the mannequin females which received an abdominal massage for the removal of the male ejaculate. The eggs of each method were marked and incubated for 18 days. There was a fertility index of 0% in method 1, and 4.88% in method 2. After failure in fertility and feasibility rates, tests for sperm detection were sought in the male reproductive system through histological cuts. The conclusion was that the methods of artificial insemination were not efficient because did not exert the necessary pressure to enable sperm. Thus, the methods of semen collection and artificial insemination applied obtained undesirable indices and are not viable for reproductive management in quails.El objetivo de este estudio fue evaluar diferentes métodos de recolección de semen en codornices y la posible viabilidad de la inseminación artificial. Se utilizaron dos métodos de colección: método 1: Masaje abdominal en el macho, y método 2: Masaje abdominal en la hembra después de la cópula masculina. Utilizamos 16 hembras y 13 machos de la especie Coturnix coturnix coturnix, con 10 semanas de edad. En el primer método, el macho seleccionado fue estimulado con la técnica de masaje abdominal, haciendo que liberara una secreción espumosa a través de las glándulas para los cloácales y su aparato reproductivo, que se recogió en una jeringa desechable de 1 ml de insulina, siendo posteriormente aplicado inmediatamente en la región vagina de cada par de hembras en el método 1. En el segundo método 2, la cópula del macho seleccionado se realizó en las hembras de maniquí, donde recibieron un masaje abdominal para la extracción de la eyaculación masculina. Los huevos de cada método fueron marcados e incubados durante 18 días. Hubo un índice de fertilidad del 0% en el método 1, y del 4,88% en el método 2. Después de fallas en las tasas de fertilidad y viabilidad, se buscaron pruebas para la detección de espermatozoides en el sistema reproductivo masculino a través de cortes histológicos. Se llegó a la conclusión preverque los métodos de inseminación artificial no eran eficientes porque no ejercían la presión necesaria para permitir los espermatozoides. A partir de cortes histológicos fue posible identificar y localizar espermatozoides en todo el sistema reproductivo en el macho específicamente en el conducto deferente. Por lo tanto, los métodos de recolección de semen e inseminación artificial aplicados obtuvieron índices indeseables, por lo que no son métodos viables de gestión reproductiva en las codornices.Objetivou-se avaliar diferentes métodos de coleta de sêmen em codornas e a possível viabilidade da inseminação artificial. Foram utilizados dois métodos de coleta: método 1: Massagem abdominal no macho, e método 2: Massagem abdominal na fêmea após a cópula do macho. Foram utilizadas 16 fêmeas e 13 machos da espécie Coturnix coturnix coturnix, com idade de 10 semanas. No primeiro método, o macho selecionado foi estimulado com a técnica massagem abdominal, fazendo-o liberar uma secreção espumosa pelas glândulas para cloacais e seu aparelho reprodutivo, a qual era coletada em uma seringa descartável de insulina de 1 ml, sendo posteriormente aplicada imediatamente na região da vagina de cada dupla de fêmeas do método 1. Já no segundo método 2, foi realizada a cópula do macho selecionado nas fêmeas manequins, onde as mesmas receberam uma massagem abdominal para a retirada do ejaculado do macho. Os ovos de cada método foram marcados e incubados durante 18 dias. Houve um índice de fertilidade de 0% no método 1, e de 4.88% no método 2. Após o insucesso nas taxas de fertilidade e viabilidade, procuraram-se os testes para detecção dos espermatozoides no sistema reprodutivo do macho através de cortes histológicos. Chegou-se à conclusão previa os métodos de inseminação artificial não foram eficientes por não exercerem a pressão necessária para viabilizar o espermatozoide. Dessa forma, os métodos de coleta de sêmen e inseminação artificial aplicado obtiveram índices indesejáveis, logo não são métodos viáveis de manejo reprodutivo em codornas.Research, Society and Development2020-03-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/278910.33448/rsd-v9i4.2789Research, Society and Development; Vol. 9 No. 4; e88942789Research, Society and Development; Vol. 9 Núm. 4; e88942789Research, Society and Development; v. 9 n. 4; e889427892525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/2789/2265Copyright (c) 2020 Felipe Cardoso Serpa, Ianglio Márcio Travassos Duarte, Ezequiel Manfio Somavila, Leonardo Faccenda Zanchin, Mabel Molinari, Rodrigo Garófallo Garcia, Jean Kaique Valentim, Maria Fernanda de Castro Burbarelli, Bruna Barreto Przybulinskiinfo:eu-repo/semantics/openAccessSerpa, Felipe CardosoJácome, Ianglio Márcio Travassos DuarteGarcia, Rodrigo GarófalloZanchin, Leonardo FaccendaSomavila, Ezequiel ManfioMolinari, MabelValentim, Jean KaiqueBurbarelli, Maria Fernanda de CastroPrzybulinski, Bruna Barreto2020-08-20T18:07:16Zoai:ojs.pkp.sfu.ca:article/2789Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:27:14.444070Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Artificial insemination in quails through different methods of semen collection Inseminación artificial en codornices usando diferentes métodos de recolección de semen Inseminação artificial em codornas através de diferentes métodos de coleta de sêmen |
title |
Artificial insemination in quails through different methods of semen collection |
spellingShingle |
Artificial insemination in quails through different methods of semen collection Serpa, Felipe Cardoso Acasalamento Aparelho reprodutivo Fertilidade Massagem Reprodução. apareamiento órganos reproductivos fertilidad masaje reproducción. Mating Reproductive appliance Fertility Massage Reproduction. |
title_short |
Artificial insemination in quails through different methods of semen collection |
title_full |
Artificial insemination in quails through different methods of semen collection |
title_fullStr |
Artificial insemination in quails through different methods of semen collection |
title_full_unstemmed |
Artificial insemination in quails through different methods of semen collection |
title_sort |
Artificial insemination in quails through different methods of semen collection |
author |
Serpa, Felipe Cardoso |
author_facet |
Serpa, Felipe Cardoso Jácome, Ianglio Márcio Travassos Duarte Garcia, Rodrigo Garófallo Zanchin, Leonardo Faccenda Somavila, Ezequiel Manfio Molinari, Mabel Valentim, Jean Kaique Burbarelli, Maria Fernanda de Castro Przybulinski, Bruna Barreto |
author_role |
author |
author2 |
Jácome, Ianglio Márcio Travassos Duarte Garcia, Rodrigo Garófallo Zanchin, Leonardo Faccenda Somavila, Ezequiel Manfio Molinari, Mabel Valentim, Jean Kaique Burbarelli, Maria Fernanda de Castro Przybulinski, Bruna Barreto |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Serpa, Felipe Cardoso Jácome, Ianglio Márcio Travassos Duarte Garcia, Rodrigo Garófallo Zanchin, Leonardo Faccenda Somavila, Ezequiel Manfio Molinari, Mabel Valentim, Jean Kaique Burbarelli, Maria Fernanda de Castro Przybulinski, Bruna Barreto |
dc.subject.por.fl_str_mv |
Acasalamento Aparelho reprodutivo Fertilidade Massagem Reprodução. apareamiento órganos reproductivos fertilidad masaje reproducción. Mating Reproductive appliance Fertility Massage Reproduction. |
topic |
Acasalamento Aparelho reprodutivo Fertilidade Massagem Reprodução. apareamiento órganos reproductivos fertilidad masaje reproducción. Mating Reproductive appliance Fertility Massage Reproduction. |
description |
The objective of this study was to evaluate different methods of semen collection in quails and the possible viability of artificial insemination. Two collection methods were used: method 1: Abdominal massage in the male, and method 2: Abdominal massage in the female after male copulation. Were used 16 females and 13 males of the species Coturnix coturnix coturnix, aged 10 weeks. In the first method, the selected male was stimulated with the abdominal massage technique to release a foamy secretion through the glands for cloacal and his reproductive apparatus, which was collected in a disposable 1 ml insulin syringe, immediately and disposed in the vagina region of each pair of females in method 1. In the second method 2, the copulation of the selected male was performed in the mannequin females which received an abdominal massage for the removal of the male ejaculate. The eggs of each method were marked and incubated for 18 days. There was a fertility index of 0% in method 1, and 4.88% in method 2. After failure in fertility and feasibility rates, tests for sperm detection were sought in the male reproductive system through histological cuts. The conclusion was that the methods of artificial insemination were not efficient because did not exert the necessary pressure to enable sperm. Thus, the methods of semen collection and artificial insemination applied obtained undesirable indices and are not viable for reproductive management in quails. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/2789 10.33448/rsd-v9i4.2789 |
url |
https://rsdjournal.org/index.php/rsd/article/view/2789 |
identifier_str_mv |
10.33448/rsd-v9i4.2789 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/2789/2265 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 4; e88942789 Research, Society and Development; Vol. 9 Núm. 4; e88942789 Research, Society and Development; v. 9 n. 4; e88942789 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052815298789376 |