Effect of laser on the biomodulation of osteogenesis in animal model
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/8294 |
Resumo: | Low power laser therapy is used as a medical and dental therapeutic resource, in order to enable its therapeutic effect on the body. Experimental tests on laboratory and animal models, with well-defined methodological criteria, suggest that low level laser therapy (LLLT - Low Level Laser Therapy) modulates several biological processes in animal models after being exposed to some trauma. This study aimed to evaluate the biomodulation of osteogenesis, using the infrared laser, in the skull cap of rats after making critical defects. The biological reactions of tissues submitted to laser were analyzed, comparing the intensity of bone neoformation, randomly distributed in four groups. Control groups (GC-I-II) did not receive a laser; the tests (GT-I-II) were treated with infrared laser, both at 2J / cm2 and at 90mW. Animal sacrifice and microscopic analysis, with HE staining, occurred in 07 and 21 days. The descriptive results showed that the groups irradiated with biomodulated laser positively affected the osteogenesis process, as they presented, at 07 and 21 days, a pattern of superior bone neoformation. The statistical analysis showed a superior bone neoformation score in both groups for 21 days, with larger areas of neoformation being observed. From the methodology used and with the radiation parameters used, in addition to the quantitative and histomorphometric descriptive results obtained in this research, it can be concluded that: LLLT can be used as an adjunct in the osteogenesis process in the repair of bone tissue. However, it is necessary to conduct clinical trials that prove its applicability. |
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Effect of laser on the biomodulation of osteogenesis in animal modelEfecto de la terapia con láser sobre la biomodulación de la osteogénesis en modelo animalEfeito da laserterapia na biomodulação da osteogênese em modelo animalBone developmentLaser therapyOsteogenesisRatsOral surgery.Desarrollo óseoTerapia por láserOsteogénesisRatasCirugía bucal.Desenvolvimento ósseoTerapia a laserOsteogêneseRatosCirurgia bucal.Low power laser therapy is used as a medical and dental therapeutic resource, in order to enable its therapeutic effect on the body. Experimental tests on laboratory and animal models, with well-defined methodological criteria, suggest that low level laser therapy (LLLT - Low Level Laser Therapy) modulates several biological processes in animal models after being exposed to some trauma. This study aimed to evaluate the biomodulation of osteogenesis, using the infrared laser, in the skull cap of rats after making critical defects. The biological reactions of tissues submitted to laser were analyzed, comparing the intensity of bone neoformation, randomly distributed in four groups. Control groups (GC-I-II) did not receive a laser; the tests (GT-I-II) were treated with infrared laser, both at 2J / cm2 and at 90mW. Animal sacrifice and microscopic analysis, with HE staining, occurred in 07 and 21 days. The descriptive results showed that the groups irradiated with biomodulated laser positively affected the osteogenesis process, as they presented, at 07 and 21 days, a pattern of superior bone neoformation. The statistical analysis showed a superior bone neoformation score in both groups for 21 days, with larger areas of neoformation being observed. From the methodology used and with the radiation parameters used, in addition to the quantitative and histomorphometric descriptive results obtained in this research, it can be concluded that: LLLT can be used as an adjunct in the osteogenesis process in the repair of bone tissue. However, it is necessary to conduct clinical trials that prove its applicability.La terapia con láser de baja potencia se utiliza como recurso terapéutico médico y dental, con el fin de permitir su efecto terapéutico en el organismo. Pruebas experimentales en modelos animales y de laboratorio, con criterios metodológicos bien definidos, sugieren que la terapia con láser de bajo nivel (LLLT - Low Level Laser Therapy) modula varios procesos biológicos en modelos animales después de haber sido expuestos a algún trauma. Este estudio tuvo como objetivo evaluar la biomodulación de la osteogénesis, utilizando el láser infrarrojo, en el casquete de ratas después de realizar defectos críticos. Se analizaron las reacciones biológicas de los tejidos sometidos a láser, comparando la intensidad de la neoformación ósea, distribuida aleatoriamente en cuatro grupos. Los grupos de control (GC-I-II) no recibieron un láser; las pruebas (GT-I-II) se trataron con láser infrarrojo, tanto a 2J / cm2 como a 90 mW. El sacrificio de animales y el análisis microscópico, con tinción HE, ocurrieron a los 07 y 21 días. Los resultados descriptivos mostraron que los grupos irradiados con láser biomodulado afectaron positivamente el proceso de osteogénesis, ya que presentaron, a los 07 y 21 días, un patrón de neoformación ósea superior. El análisis estadístico mostró una puntuación de neoformación ósea superior en ambos grupos durante 21 días, observándose áreas más grandes de neoformación. De la metodología empleada y con los parámetros de radiación utilizados, además de los resultados descriptivos cuantitativos e histomorfométricos obtenidos en esta investigación, se puede concluir que: La TLBI puede utilizarse como coadyuvante en el proceso de osteogénesis en la reparación del tejido óseo. Sin embargo, es necesario realizar ensayos clínicos que demuestren su aplicabilidad.A laserterapia de baixa potência é utilizada como recurso terapêutico médico e odontológico, com intuito de possibilitar seu efeito terapêutico sobre o organismo. Ensaios experimentais tanto laboratoriais como em modelo animal, com critério metodológico bem delimitado, sugerem que a laserterapia de baixa potência (LLLT – Low Level Laser Therapy) modula diversos processos biológicos em modelos animais depois de expostos a algum trauma. Este estudo teve como objetivo avaliar a biomodulação da osteogênese através do laser infravermelho, na calota craniana de ratos após a confecção de defeitos críticos. Procedeu-se à análise das reações biológicas dos tecidos submetidos ao laser, comparando-se a intensidade de neoformação óssea, distribuídos aleatoriamente em quatro grupos. Os grupos controles (GC-I-II) não receberam laser; os testes (GT-I-II), foram tratados com laser infravermelho, em ambos 2J/cm2 e 90mW. O sacrifício dos animais e as análises microscópicas, com coloração de HE, aconteceram em no tempo de 07 e também 21 dias. Os resultados descritivos mostraram que os grupos irradiados com laser biomodularam positivamente o processo da osteogênese, pois apresentaram, aos 07 e aos 21 dias, um padrão de neoformação óssea superior. A análise estatística demonstrou um escore de neoformação óssea superior em ambos grupos de 21 dias, observando-se maiores áreas de neoformação. A partir da metodologia empregada e com os parâmetros de radiação utilizados, além dos resultados descritivos quantitativos e histomorfométricos obtidos nesta pesquisa, pode-se concluir que: a LLLT pode ser utilizada como coadjuvante no processo de osteogênese no reparo do tecido ósseo. Entretanto, faz-se necessário a realização de ensaios clínicos que comprovem a sua aplicabilidade.Research, Society and Development2020-09-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/829410.33448/rsd-v9i9.8294Research, Society and Development; Vol. 9 No. 9; e926998294Research, Society and Development; Vol. 9 Núm. 9; e926998294Research, Society and Development; v. 9 n. 9; e9269982942525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/8294/7203Copyright (c) 2020 André Luiz Marinho Falcão Gondim; Wagner Ranier Maciel Dantas; Euler Maciel Dantas; Henrique Telles Ramos de Oliveira; Luis Ferreira de Almeida Neto; Karolina Pires Marcelino; Rogério Miranda Pagnoncellihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGondim, André Luiz Marinho FalcãoDantas, Wagner Ranier MacielDantas, Euler MacielOliveira, Henrique Telles Ramos deAlmeida Neto, Luis Ferreira deMarcelino, Karolina PiresPagnoncelli, Rogério Miranda2020-09-18T01:42:11Zoai:ojs.pkp.sfu.ca:article/8294Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:30:48.113947Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Effect of laser on the biomodulation of osteogenesis in animal model Efecto de la terapia con láser sobre la biomodulación de la osteogénesis en modelo animal Efeito da laserterapia na biomodulação da osteogênese em modelo animal |
title |
Effect of laser on the biomodulation of osteogenesis in animal model |
spellingShingle |
Effect of laser on the biomodulation of osteogenesis in animal model Gondim, André Luiz Marinho Falcão Bone development Laser therapy Osteogenesis Rats Oral surgery. Desarrollo óseo Terapia por láser Osteogénesis Ratas Cirugía bucal. Desenvolvimento ósseo Terapia a laser Osteogênese Ratos Cirurgia bucal. |
title_short |
Effect of laser on the biomodulation of osteogenesis in animal model |
title_full |
Effect of laser on the biomodulation of osteogenesis in animal model |
title_fullStr |
Effect of laser on the biomodulation of osteogenesis in animal model |
title_full_unstemmed |
Effect of laser on the biomodulation of osteogenesis in animal model |
title_sort |
Effect of laser on the biomodulation of osteogenesis in animal model |
author |
Gondim, André Luiz Marinho Falcão |
author_facet |
Gondim, André Luiz Marinho Falcão Dantas, Wagner Ranier Maciel Dantas, Euler Maciel Oliveira, Henrique Telles Ramos de Almeida Neto, Luis Ferreira de Marcelino, Karolina Pires Pagnoncelli, Rogério Miranda |
author_role |
author |
author2 |
Dantas, Wagner Ranier Maciel Dantas, Euler Maciel Oliveira, Henrique Telles Ramos de Almeida Neto, Luis Ferreira de Marcelino, Karolina Pires Pagnoncelli, Rogério Miranda |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gondim, André Luiz Marinho Falcão Dantas, Wagner Ranier Maciel Dantas, Euler Maciel Oliveira, Henrique Telles Ramos de Almeida Neto, Luis Ferreira de Marcelino, Karolina Pires Pagnoncelli, Rogério Miranda |
dc.subject.por.fl_str_mv |
Bone development Laser therapy Osteogenesis Rats Oral surgery. Desarrollo óseo Terapia por láser Osteogénesis Ratas Cirugía bucal. Desenvolvimento ósseo Terapia a laser Osteogênese Ratos Cirurgia bucal. |
topic |
Bone development Laser therapy Osteogenesis Rats Oral surgery. Desarrollo óseo Terapia por láser Osteogénesis Ratas Cirugía bucal. Desenvolvimento ósseo Terapia a laser Osteogênese Ratos Cirurgia bucal. |
description |
Low power laser therapy is used as a medical and dental therapeutic resource, in order to enable its therapeutic effect on the body. Experimental tests on laboratory and animal models, with well-defined methodological criteria, suggest that low level laser therapy (LLLT - Low Level Laser Therapy) modulates several biological processes in animal models after being exposed to some trauma. This study aimed to evaluate the biomodulation of osteogenesis, using the infrared laser, in the skull cap of rats after making critical defects. The biological reactions of tissues submitted to laser were analyzed, comparing the intensity of bone neoformation, randomly distributed in four groups. Control groups (GC-I-II) did not receive a laser; the tests (GT-I-II) were treated with infrared laser, both at 2J / cm2 and at 90mW. Animal sacrifice and microscopic analysis, with HE staining, occurred in 07 and 21 days. The descriptive results showed that the groups irradiated with biomodulated laser positively affected the osteogenesis process, as they presented, at 07 and 21 days, a pattern of superior bone neoformation. The statistical analysis showed a superior bone neoformation score in both groups for 21 days, with larger areas of neoformation being observed. From the methodology used and with the radiation parameters used, in addition to the quantitative and histomorphometric descriptive results obtained in this research, it can be concluded that: LLLT can be used as an adjunct in the osteogenesis process in the repair of bone tissue. However, it is necessary to conduct clinical trials that prove its applicability. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/8294 10.33448/rsd-v9i9.8294 |
url |
https://rsdjournal.org/index.php/rsd/article/view/8294 |
identifier_str_mv |
10.33448/rsd-v9i9.8294 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/8294/7203 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 9; e926998294 Research, Society and Development; Vol. 9 Núm. 9; e926998294 Research, Society and Development; v. 9 n. 9; e926998294 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052659119685632 |