Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro

Detalhes bibliográficos
Autor(a) principal: Silva, Jadyellen Rondon e
Data de Publicação: 2023
Outros Autores: Souza, Anderson de Oliveira, Anjos, Italo Curvelo dos, Pádua, Geilly Mara Silva de, Vieira, Lucas Campos Curcino, Gai, Bibiana Mozzaquatro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/39541
Resumo: Reactive oxygen species (ROS), when in excess, cause damage to biomolecules (DNA, lipids, carbohydrates, and proteins), which are related to several pathologies. There is a growing study of substances that act to inhibit or reduce the action of these ROS. Chalcones are aromatic ketones with a naturally occurring and easily synthesized α,β-unsaturated system. In this work, we investigated the in vitro protective action of a new class of chalcones functionalized by the thiobarbituric acid reactive substances (TBARS) assay. The most promising molecule (Chalcone E) was submitted to the DPPH radical scavenging assay, and the MTT cell viability test evaluated its cytotoxicity. In addition, Chalcone E reduced lipid peroxidation, with maximum inhibition values of 73.05% for the brain, 81.42% for the liver, and 87.23% for the kidney, demonstrating a potential antioxidant effect. Still, the DPPH test did not observe this effect, suggesting further investigation of this molecule.
id UNIFEI_07cbac1560f46982bce2faf8ed0bfd75
oai_identifier_str oai:ojs.pkp.sfu.ca:article/39541
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitroEvaluación de derivados de chalconas en la reducción de la peroxidación lipídica inducida por Fe2+/EDTA in vitroAvaliação de derivados de chalconas na redução da peroxidação lipídica induzida por Fe2+/EDTA in vitroChalconasEspecies reactivasAntioxidanteHepatoprotectorPeroxidación lipídica.ChalconesReactive speciesAntioxidantHepatoprotectiveLipid peroxidation.ChalconasEspécies reativasAntioxidanteHepatoprotetoraPeroxidação lipídica.Reactive oxygen species (ROS), when in excess, cause damage to biomolecules (DNA, lipids, carbohydrates, and proteins), which are related to several pathologies. There is a growing study of substances that act to inhibit or reduce the action of these ROS. Chalcones are aromatic ketones with a naturally occurring and easily synthesized α,β-unsaturated system. In this work, we investigated the in vitro protective action of a new class of chalcones functionalized by the thiobarbituric acid reactive substances (TBARS) assay. The most promising molecule (Chalcone E) was submitted to the DPPH radical scavenging assay, and the MTT cell viability test evaluated its cytotoxicity. In addition, Chalcone E reduced lipid peroxidation, with maximum inhibition values of 73.05% for the brain, 81.42% for the liver, and 87.23% for the kidney, demonstrating a potential antioxidant effect. Still, the DPPH test did not observe this effect, suggesting further investigation of this molecule.Las especies reactivas de oxígeno (EROs), en exceso, provocan daños en biomoléculas (ADN, lípidos, carbohidratos y proteínas), las cuales se relacionan con diversas patologías. Existe un creciente estudio de sustancias que actúan para inhibir o reducir la acción de estas EROs. Las chalconas son cetonas aromáticas con un sistema α,β-insaturado natural y fácilmente sintetizado. En este trabajo, investigamos la acción protector in vitro de una nueva clase de chalconas funcionalizadas por el ensayo de sustancias reactivas al ácido tiobarbitúrico (TBARS). La molécula más prometedora (Chalcone E) se sometió al ensayo de eliminación de radicales DPPH y la prueba de viabilidad celular MTT evaluó su citotoxicidad. Además, Chalcone E redujo la peroxidación lipídica, con valores máximos de inhibición del 73,05 % para el cerebro, 81,42 % para el hígado y 87,23 % para el riñón, demostrando un potencial efecto antioxidante. Aún así, la prueba DPPH no observó este efecto, lo que sugiere una mayor investigación de esta molécula.As espécies reativas do oxigênio (EROs), quando em excesso, ocasionam danos a biomoléculas (DNA, lipídeos, carboidratos e proteínas) que estão relacionadas a diversas patologias. Diante disso, aumentou-se a busca por substâncias capazes de reduzir ou inibir a ação destas espécies. Chalconas são cetonas aromáticas com sistema α,β- insaturado, de ocorrência natural e facilmente sintetizada. Neste trabalho, investigamos a atividade protetora in vitro de uma nova classe de chalconas funcionalizadas pelo teste de Substâncias Reativas ao Ácido Tiobarbitúrico (TBARS). A molécula mais promissora (Chalcona E) foi submetida ao teste de sequestro do radical DPPH e toxicidade através da viabilidade celular pelo método de MTT. Ademais, a Chalcona E foi eficiente em reduzir a peroxidação lipídica com valores de inibição máxima de 73,05% para o cérebro, 81,42% para o fígado e 87,23% para o rim, demonstrando um potencial efeito antioxidante. Ainda assim, o teste DPPH não observou esse efeito, sugerindo uma investigação mais aprofundada dessa molécula.Research, Society and Development2023-01-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3954110.33448/rsd-v12i1.39541Research, Society and Development; Vol. 12 No. 1; e16012139541Research, Society and Development; Vol. 12 Núm. 1; e16012139541Research, Society and Development; v. 12 n. 1; e160121395412525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/39541/32489Copyright (c) 2023 Jadyellen Rondon e Silva; Anderson de Oliveira Souza; Italo Curvelo dos Anjos; Geilly Mara Silva de Pádua; Lucas Campos Curcino Vieira; Bibiana Mozzaquatro Gaihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Jadyellen Rondon e Souza, Anderson de Oliveira Anjos, Italo Curvelo dos Pádua, Geilly Mara Silva de Vieira, Lucas Campos Curcino Gai, Bibiana Mozzaquatro 2023-01-13T10:30:42Zoai:ojs.pkp.sfu.ca:article/39541Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-01-13T10:30:42Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro
Evaluación de derivados de chalconas en la reducción de la peroxidación lipídica inducida por Fe2+/EDTA in vitro
Avaliação de derivados de chalconas na redução da peroxidação lipídica induzida por Fe2+/EDTA in vitro
title Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro
spellingShingle Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro
Silva, Jadyellen Rondon e
Chalconas
Especies reactivas
Antioxidante
Hepatoprotector
Peroxidación lipídica.
Chalcones
Reactive species
Antioxidant
Hepatoprotective
Lipid peroxidation.
Chalconas
Espécies reativas
Antioxidante
Hepatoprotetora
Peroxidação lipídica.
title_short Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro
title_full Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro
title_fullStr Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro
title_full_unstemmed Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro
title_sort Evaluation of chalcones derivatives in lipid peroxidation reduction induced by Fe2+/EDTA in vitro
author Silva, Jadyellen Rondon e
author_facet Silva, Jadyellen Rondon e
Souza, Anderson de Oliveira
Anjos, Italo Curvelo dos
Pádua, Geilly Mara Silva de
Vieira, Lucas Campos Curcino
Gai, Bibiana Mozzaquatro
author_role author
author2 Souza, Anderson de Oliveira
Anjos, Italo Curvelo dos
Pádua, Geilly Mara Silva de
Vieira, Lucas Campos Curcino
Gai, Bibiana Mozzaquatro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Jadyellen Rondon e
Souza, Anderson de Oliveira
Anjos, Italo Curvelo dos
Pádua, Geilly Mara Silva de
Vieira, Lucas Campos Curcino
Gai, Bibiana Mozzaquatro
dc.subject.por.fl_str_mv Chalconas
Especies reactivas
Antioxidante
Hepatoprotector
Peroxidación lipídica.
Chalcones
Reactive species
Antioxidant
Hepatoprotective
Lipid peroxidation.
Chalconas
Espécies reativas
Antioxidante
Hepatoprotetora
Peroxidação lipídica.
topic Chalconas
Especies reactivas
Antioxidante
Hepatoprotector
Peroxidación lipídica.
Chalcones
Reactive species
Antioxidant
Hepatoprotective
Lipid peroxidation.
Chalconas
Espécies reativas
Antioxidante
Hepatoprotetora
Peroxidação lipídica.
description Reactive oxygen species (ROS), when in excess, cause damage to biomolecules (DNA, lipids, carbohydrates, and proteins), which are related to several pathologies. There is a growing study of substances that act to inhibit or reduce the action of these ROS. Chalcones are aromatic ketones with a naturally occurring and easily synthesized α,β-unsaturated system. In this work, we investigated the in vitro protective action of a new class of chalcones functionalized by the thiobarbituric acid reactive substances (TBARS) assay. The most promising molecule (Chalcone E) was submitted to the DPPH radical scavenging assay, and the MTT cell viability test evaluated its cytotoxicity. In addition, Chalcone E reduced lipid peroxidation, with maximum inhibition values of 73.05% for the brain, 81.42% for the liver, and 87.23% for the kidney, demonstrating a potential antioxidant effect. Still, the DPPH test did not observe this effect, suggesting further investigation of this molecule.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/39541
10.33448/rsd-v12i1.39541
url https://rsdjournal.org/index.php/rsd/article/view/39541
identifier_str_mv 10.33448/rsd-v12i1.39541
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/39541/32489
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 12 No. 1; e16012139541
Research, Society and Development; Vol. 12 Núm. 1; e16012139541
Research, Society and Development; v. 12 n. 1; e16012139541
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052615443349504

Registros relacionados