Heat stress in sugarcane: physiological changes and gene expression

Detalhes bibliográficos
Autor(a) principal: Panta, Airan Miguel dos Santos
Data de Publicação: 2022
Outros Autores: Souza, Juliana Lopes, Gagliardi, Paulo Roberto, Oliveira Junior, Luiz Fernando Ganassali, Fontes, Pryanka Thuyra Nascimento, Fagundes, Jailson Lara, Silva-Mann, Renata
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/26260
Resumo: Facing climate change is necessary to know the effects of temperature on plant development and identify which physiological factors are related to the reduction and/or increase in photosynthesis. This identification can be made in the first stages of development. Thus, the objective was to evaluate the physiological parameters of the sugarcane cultivar RB 86 7515 at 15°C, 28°C, and 40°C at different exposure times (0, 4, 8, 12, and 24 hours) and to evaluate 18S gene expression. The experiment was established in a completely randomized design, with six replications. Chlorophyll a and b were measured, and the a/b ratio, total chlorophyll in leaves, and variables related to photosystems. Initial (F0) and maximum (Fm) fluorescence values ​​were measured. The ratio Fv/Fo and the potential quantum efficiency of photosystem II (PSII) Fv/Fm were estimated. The mRNA was extracted using the same types of leaves used in the photosynthesis assessment. High temperatures with exposure for 24 hours affect the photosynthetic efficiency of sugarcane plants. Exposure of sugarcane plants with 65 days of development to 40 ºC for 24 hours is recommended to assess tolerance to temperature stress, aiming at early selection of genotypes.
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spelling Heat stress in sugarcane: physiological changes and gene expressionEstrés calórico en caña de azúcar: cambios fisiológicos y expresión génicaEstresse térmico em cana-de-açúcar: mudanças fisiológicas e expressão gênica OJIPClorofilaFluorescenciaARNFotosistema.OJIPChlorophyllFluorescenceRNAPhotosystem.OJIPClorofilaFluorescênciaRNAFotossistema.Facing climate change is necessary to know the effects of temperature on plant development and identify which physiological factors are related to the reduction and/or increase in photosynthesis. This identification can be made in the first stages of development. Thus, the objective was to evaluate the physiological parameters of the sugarcane cultivar RB 86 7515 at 15°C, 28°C, and 40°C at different exposure times (0, 4, 8, 12, and 24 hours) and to evaluate 18S gene expression. The experiment was established in a completely randomized design, with six replications. Chlorophyll a and b were measured, and the a/b ratio, total chlorophyll in leaves, and variables related to photosystems. Initial (F0) and maximum (Fm) fluorescence values ​​were measured. The ratio Fv/Fo and the potential quantum efficiency of photosystem II (PSII) Fv/Fm were estimated. The mRNA was extracted using the same types of leaves used in the photosynthesis assessment. High temperatures with exposure for 24 hours affect the photosynthetic efficiency of sugarcane plants. Exposure of sugarcane plants with 65 days of development to 40 ºC for 24 hours is recommended to assess tolerance to temperature stress, aiming at early selection of genotypes.Ante el cambio climático, es necesario conocer los efectos de la temperatura en el desarrollo de las plantas, e identificar qué factores fisiológicos están relacionados con la reducción y/o aumento de la fotosíntesis. Esta identificación puede hacerse en las primeras etapas de desarrollo. Así, el objetivo fue evaluar parámetros fisiológicos del cultivar de caña de azúcar RB 86 7515 a 15°C, 28°C y 40°C a diferentes tiempos de exposición (0, 4, 8, 12 y 24 horas) y evaluar la expresión del gen 18S. El experimento se estableció en un diseño completamente al azar, con seis repeticiones. Se midieron clorofila a y b, y se estimó la relación a/b, clorofila total en hojas y variables relacionadas con los fotosistemas. Se midieron los valores de fluorescencia inicial (F0) y máxima (Fm). Con estas variables en mente, se estimaron las relaciones Fv/Fo y la eficiencia cuántica potencial del fotosistema II (PSII) Fv/Fm. El ARNm se extrajo usando los mismos tipos de hojas que se usaron en la evaluación de la fotosíntesis. Las altas temperaturas con exposición durante 24 horas afectan la eficiencia fotosintética de las plantas de caña de azúcar. Se recomienda la exposición de plantas de caña de azúcar con 65 días de desarrollo a 40ºC por 24 horas para evaluar la tolerancia al estrés por temperatura, visando la selección temprana de genotipos.Frente às mudanças climáticas faz-se necessário conhecer os efeitos da temperatura no desenvolvimento das plantas, e identificar quais fatores fisiológicos estão relacionados à redução e/ou aumento da fotossíntese. Esta identificação pode ser feita nos primeiros estádios de desenvolvimento. Assim, objetivou-se avaliar parâmetros fisiológicos da cultivar de cana-de-açúcar RB 86 7515 a 15°C, 28°C e 40°C em diferentes tempos de exposição (0, 4, 8, 12 e 24 horas) e avaliar a expressão gênica 18S. O experimento foi estabelecido em delineamento inteiramente casualizado, com seis repetições. Foram feitas as medidas de clorofila a e b, e estimada a relação a/b, e o total de clorofila nas folhas, e variáveis relacionadas aos fotossistemas. Foram medidos os valores de fluorescência inicial (F0) e máxima (Fm). De posse dessas variáveis foram estimadas as relações: Fv/Fo e a eficiência quântica potencial do fotossistema II (FSII) Fv/Fm. O RNAm foi extraído usando os mesmos tipos de folhas usadas na avaliação da fotossíntese. Altas temperaturas com exposição por 24 horas afetam a eficiência fotossintética das plantas de cana-de-açúcar. Exposição de plantas de cana-de-açúcar com 65 dias de desenvolvimento a 40 ºC por 24 horas é recomendada para avaliação da tolerância ao estresse por temperatura, visando a seleção precoce de genótipos.Research, Society and Development2022-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2626010.33448/rsd-v11i3.26260Research, Society and Development; Vol. 11 No. 3; e15511326260Research, Society and Development; Vol. 11 Núm. 3; e15511326260Research, Society and Development; v. 11 n. 3; e155113262602525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/26260/23102Copyright (c) 2022 Airan Miguel dos Santos Panta; Juliana Lopes Souza; Paulo Roberto Gagliardi; Luiz Fernando Ganassali Oliveira Junior; Pryanka Thuyra Nascimento Fontes; Jailson Lara Fagundes; Renata Silva-Mannhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPanta, Airan Miguel dos Santos Souza, Juliana Lopes Gagliardi, Paulo Roberto Oliveira Junior, Luiz Fernando Ganassali Fontes, Pryanka Thuyra Nascimento Fagundes, Jailson Lara Silva-Mann, Renata 2022-03-09T13:44:38Zoai:ojs.pkp.sfu.ca:article/26260Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:44:20.766800Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Heat stress in sugarcane: physiological changes and gene expression
Estrés calórico en caña de azúcar: cambios fisiológicos y expresión génica
Estresse térmico em cana-de-açúcar: mudanças fisiológicas e expressão gênica
title Heat stress in sugarcane: physiological changes and gene expression
spellingShingle Heat stress in sugarcane: physiological changes and gene expression
Panta, Airan Miguel dos Santos
OJIP
Clorofila
Fluorescencia
ARN
Fotosistema.
OJIP
Chlorophyll
Fluorescence
RNA
Photosystem.
OJIP
Clorofila
Fluorescência
RNA
Fotossistema.
title_short Heat stress in sugarcane: physiological changes and gene expression
title_full Heat stress in sugarcane: physiological changes and gene expression
title_fullStr Heat stress in sugarcane: physiological changes and gene expression
title_full_unstemmed Heat stress in sugarcane: physiological changes and gene expression
title_sort Heat stress in sugarcane: physiological changes and gene expression
author Panta, Airan Miguel dos Santos
author_facet Panta, Airan Miguel dos Santos
Souza, Juliana Lopes
Gagliardi, Paulo Roberto
Oliveira Junior, Luiz Fernando Ganassali
Fontes, Pryanka Thuyra Nascimento
Fagundes, Jailson Lara
Silva-Mann, Renata
author_role author
author2 Souza, Juliana Lopes
Gagliardi, Paulo Roberto
Oliveira Junior, Luiz Fernando Ganassali
Fontes, Pryanka Thuyra Nascimento
Fagundes, Jailson Lara
Silva-Mann, Renata
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Panta, Airan Miguel dos Santos
Souza, Juliana Lopes
Gagliardi, Paulo Roberto
Oliveira Junior, Luiz Fernando Ganassali
Fontes, Pryanka Thuyra Nascimento
Fagundes, Jailson Lara
Silva-Mann, Renata
dc.subject.por.fl_str_mv OJIP
Clorofila
Fluorescencia
ARN
Fotosistema.
OJIP
Chlorophyll
Fluorescence
RNA
Photosystem.
OJIP
Clorofila
Fluorescência
RNA
Fotossistema.
topic OJIP
Clorofila
Fluorescencia
ARN
Fotosistema.
OJIP
Chlorophyll
Fluorescence
RNA
Photosystem.
OJIP
Clorofila
Fluorescência
RNA
Fotossistema.
description Facing climate change is necessary to know the effects of temperature on plant development and identify which physiological factors are related to the reduction and/or increase in photosynthesis. This identification can be made in the first stages of development. Thus, the objective was to evaluate the physiological parameters of the sugarcane cultivar RB 86 7515 at 15°C, 28°C, and 40°C at different exposure times (0, 4, 8, 12, and 24 hours) and to evaluate 18S gene expression. The experiment was established in a completely randomized design, with six replications. Chlorophyll a and b were measured, and the a/b ratio, total chlorophyll in leaves, and variables related to photosystems. Initial (F0) and maximum (Fm) fluorescence values ​​were measured. The ratio Fv/Fo and the potential quantum efficiency of photosystem II (PSII) Fv/Fm were estimated. The mRNA was extracted using the same types of leaves used in the photosynthesis assessment. High temperatures with exposure for 24 hours affect the photosynthetic efficiency of sugarcane plants. Exposure of sugarcane plants with 65 days of development to 40 ºC for 24 hours is recommended to assess tolerance to temperature stress, aiming at early selection of genotypes.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/26260
10.33448/rsd-v11i3.26260
url https://rsdjournal.org/index.php/rsd/article/view/26260
identifier_str_mv 10.33448/rsd-v11i3.26260
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/26260/23102
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 3; e15511326260
Research, Society and Development; Vol. 11 Núm. 3; e15511326260
Research, Society and Development; v. 11 n. 3; e15511326260
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052704691847168

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