Influence of maternal hyperglycemia on rat daughters and granddaughters
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/41798 |
Resumo: | This study evaluated the influence of maternal diabetes on the reproductive outcomes of adult daughters during full-term pregnancy in rats. The local ethics committee approved all experimental protocols. The adult female pups of nondiabetic (control – CP) and diabetic (DP) mothers were subjected to mating with healthy male rats for reproductive analysis. On day 17 of pregnancy, both groups underwent an oral glucose tolerance test (OGTT) to evaluate glucose tolerance. At the end of pregnancy, the rats were anesthetized for laparotomy and exposure of maternal organs. Uterine horns, ovaries, fetuses, and placentas were weighed to classify fetal weight and placental efficiency. P<0.05 was considered a statistically significant limit, and Pearson's correlation was used. Higher maternal fasting blood glucose was correlated with a lower number of live fetuses and, consequently, with a lower litter weight. Thus, maternal fasting glucose during pregnancy is a relevant biomarker related to the poor reproductive outcome of daughters of rats. Furthermore, these findings show the detrimental effects of fetal programming induced by maternal diabetes in adulthood in the subsequent generation. |
id |
UNIFEI_0b75f9bcf29d19e7077ed56ff8fa82dc |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/41798 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Influence of maternal hyperglycemia on rat daughters and granddaughtersInfluencia de la hiperglucemia materna en hijas y nietas de ratasInfluência da hiperglicemia maternal nas filhas e netas de ratasDiabetesRatasResultados reproductivosFetoEmbarazo.DiabetesRatsReproductive outcomesFetusPregnancy.DiabetesRatosResultados reprodutivosFetoGravidez.This study evaluated the influence of maternal diabetes on the reproductive outcomes of adult daughters during full-term pregnancy in rats. The local ethics committee approved all experimental protocols. The adult female pups of nondiabetic (control – CP) and diabetic (DP) mothers were subjected to mating with healthy male rats for reproductive analysis. On day 17 of pregnancy, both groups underwent an oral glucose tolerance test (OGTT) to evaluate glucose tolerance. At the end of pregnancy, the rats were anesthetized for laparotomy and exposure of maternal organs. Uterine horns, ovaries, fetuses, and placentas were weighed to classify fetal weight and placental efficiency. P<0.05 was considered a statistically significant limit, and Pearson's correlation was used. Higher maternal fasting blood glucose was correlated with a lower number of live fetuses and, consequently, with a lower litter weight. Thus, maternal fasting glucose during pregnancy is a relevant biomarker related to the poor reproductive outcome of daughters of rats. Furthermore, these findings show the detrimental effects of fetal programming induced by maternal diabetes in adulthood in the subsequent generation.Este estudio tuvo como objetivo evaluar la influencia de la diabetes materna en el desempeño reproductivo de sus hijas adultas durante el embarazo a término en ratas. El comité de ética local aprobó todos los protocolos del estudio. Las crías hembras adultas de madres no diabéticas (control - HC) y moderadamente diabéticas (HD) se sometieron a apareamiento con machos sanos para el análisis de los resultados reproductivos. El día 17 del embarazo, ambos grupos se sometieron a una prueba de tolerancia a la glucosa oral (OGTT) para evaluar la tolerancia a la glucosa y, al final del embarazo, las ratas fueron anestesiadas para laparotomía y exposición de los órganos maternos. Se pesaron los cuernos uterinos, los ovarios, los fetos y las placentas para clasificar el peso fetal y la eficiencia placentaria. P<0,05 fue considerado como el límite estadísticamente significativo y se utilizó la correlación de Pearson. Una mayor glucemia materna en ayunas se correlacionó con un menor número de fetos vivos y, en consecuencia, con un menor peso de la camada. Por lo tanto, la glucosa materna en ayunas durante el embarazo es un biomarcador relevante relacionado con el deterioro del resultado reproductivo de las hijas de ratas. Además, estos hallazgos muestran los efectos perjudiciales de la programación fetal inducida por la diabetes materna en la edad adulta en la generación siguiente.Este estudo teve como objetivo avaliar a influência do diabete materno no desempenho reprodutivo de suas filhas adultas na prenhez a termo em ratas. O Comitê de ética local aprovou todos os protocolos do estudo. A prole feminina adulta de mães não diabéticas (controle – FC) e diabete moderada (FD) foram submetidas a acasalamento com machos saudáveis para análise dos resultados reprodutivos. No 17º dia de gestação, ambos os grupos foram submetidos a um teste oral de tolerância à glicose (TOTG) para avaliação da tolerância à glicose e, ao final de prenhez, as ratas foram anestesiadas para laparotomia e exposição dos órgãos maternos. Os cornos uterinos, ovários, fetos e placentas foram pesados para classificação do peso fetal e eficiência placentária. P<0,05 foi considerado o limite estatisticamente significativo, e a correlação de Pearson foi usada. A maior glicemia materna em jejum correlacionou-se com o menor número de fetos vivos e, consequentemente, com menor peso da ninhada. Assim, a glicemia de jejum materna durante a gravidez é um biomarcador relevante relacionado ao comprometimento do resultado reprodutivo de filhas de ratas. Além disso, esses achados mostram os efeitos prejudiciais da programação fetal induzida pelo diabete materno na idade adulta da geração sucessiva.Research, Society and Development2023-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4179810.33448/rsd-v12i6.41798Research, Society and Development; Vol. 12 No. 6; e0812641798Research, Society and Development; Vol. 12 Núm. 6; e0812641798Research, Society and Development; v. 12 n. 6; e08126417982525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/41798/33969Copyright (c) 2023 Luigi M. Jeuken Di Domizio; Maria Eduarda Picolo Gomes; Verônyca Gonçalves Paula; Maysa Rocha de Souza; Franciane Quintanilha Gallego; Vinícius Soares Barco; Débora Cristina Damascenohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDi Domizio, Luigi M. Jeuken Gomes, Maria Eduarda Picolo Paula, Verônyca Gonçalves Souza, Maysa Rocha de Gallego, Franciane Quintanilha Barco, Vinícius Soares Damasceno, Débora Cristina 2023-07-06T11:16:27Zoai:ojs.pkp.sfu.ca:article/41798Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-07-06T11:16:27Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Influence of maternal hyperglycemia on rat daughters and granddaughters Influencia de la hiperglucemia materna en hijas y nietas de ratas Influência da hiperglicemia maternal nas filhas e netas de ratas |
title |
Influence of maternal hyperglycemia on rat daughters and granddaughters |
spellingShingle |
Influence of maternal hyperglycemia on rat daughters and granddaughters Di Domizio, Luigi M. Jeuken Diabetes Ratas Resultados reproductivos Feto Embarazo. Diabetes Rats Reproductive outcomes Fetus Pregnancy. Diabetes Ratos Resultados reprodutivos Feto Gravidez. |
title_short |
Influence of maternal hyperglycemia on rat daughters and granddaughters |
title_full |
Influence of maternal hyperglycemia on rat daughters and granddaughters |
title_fullStr |
Influence of maternal hyperglycemia on rat daughters and granddaughters |
title_full_unstemmed |
Influence of maternal hyperglycemia on rat daughters and granddaughters |
title_sort |
Influence of maternal hyperglycemia on rat daughters and granddaughters |
author |
Di Domizio, Luigi M. Jeuken |
author_facet |
Di Domizio, Luigi M. Jeuken Gomes, Maria Eduarda Picolo Paula, Verônyca Gonçalves Souza, Maysa Rocha de Gallego, Franciane Quintanilha Barco, Vinícius Soares Damasceno, Débora Cristina |
author_role |
author |
author2 |
Gomes, Maria Eduarda Picolo Paula, Verônyca Gonçalves Souza, Maysa Rocha de Gallego, Franciane Quintanilha Barco, Vinícius Soares Damasceno, Débora Cristina |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Di Domizio, Luigi M. Jeuken Gomes, Maria Eduarda Picolo Paula, Verônyca Gonçalves Souza, Maysa Rocha de Gallego, Franciane Quintanilha Barco, Vinícius Soares Damasceno, Débora Cristina |
dc.subject.por.fl_str_mv |
Diabetes Ratas Resultados reproductivos Feto Embarazo. Diabetes Rats Reproductive outcomes Fetus Pregnancy. Diabetes Ratos Resultados reprodutivos Feto Gravidez. |
topic |
Diabetes Ratas Resultados reproductivos Feto Embarazo. Diabetes Rats Reproductive outcomes Fetus Pregnancy. Diabetes Ratos Resultados reprodutivos Feto Gravidez. |
description |
This study evaluated the influence of maternal diabetes on the reproductive outcomes of adult daughters during full-term pregnancy in rats. The local ethics committee approved all experimental protocols. The adult female pups of nondiabetic (control – CP) and diabetic (DP) mothers were subjected to mating with healthy male rats for reproductive analysis. On day 17 of pregnancy, both groups underwent an oral glucose tolerance test (OGTT) to evaluate glucose tolerance. At the end of pregnancy, the rats were anesthetized for laparotomy and exposure of maternal organs. Uterine horns, ovaries, fetuses, and placentas were weighed to classify fetal weight and placental efficiency. P<0.05 was considered a statistically significant limit, and Pearson's correlation was used. Higher maternal fasting blood glucose was correlated with a lower number of live fetuses and, consequently, with a lower litter weight. Thus, maternal fasting glucose during pregnancy is a relevant biomarker related to the poor reproductive outcome of daughters of rats. Furthermore, these findings show the detrimental effects of fetal programming induced by maternal diabetes in adulthood in the subsequent generation. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/41798 10.33448/rsd-v12i6.41798 |
url |
https://rsdjournal.org/index.php/rsd/article/view/41798 |
identifier_str_mv |
10.33448/rsd-v12i6.41798 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/41798/33969 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 12 No. 6; e0812641798 Research, Society and Development; Vol. 12 Núm. 6; e0812641798 Research, Society and Development; v. 12 n. 6; e0812641798 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052624898359296 |