In silico studies, chemical composition, antibacterial activity and in vitro antigen-induced phagocytosis of Stryphnodendron adstringens (Mart.) Coville

• Autor(a) principal: Bueno, Paulo Inácio
• Data de Publicação: 2022
• Outros Autores: Machado, Daisy, Lancellotti, Marcelo, Gonçalves, Carolina Passarelli, Marcucci, Maria Cristina, Sawaya, Alexandra Christine Helena Frankland, Melo, Adriana de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/25748
• topic: Stryphnodendron adstringens; UHPLC-MS; Lytic function; Antimicrobial activity; Phagocytosis.; Stryphnodendron adstringens; UHPLC-MS; Atividade antimicrobiana; Função lítica; Fagocitose.; Stryphnodendron adstringens; UHPLC-MS; Actividad antimicrobiana; Función lítica; Fagocitosis.

We investigate the chemical composition of aqueous and hydroethanolic extracts of Stryphnodendron adstringens by evaluating the total polyphenolic and tannin contents, antioxidant activity as well as marker compounds by UHPLC-MS. Through the in silico approach the molecular and pharmacokinetic parameters were predicted for the two major substances found in the extract, the pyrogallol (1) and chlorogenic acid (2). The antibacterial activity was verified by determining the MIC of the extracts on different bacterial strains. The inhibition zone diameter (ZD) of three different extracts of S. adstringens was studied, those with ZD>10mm proceeded to the determination of the MICs. The most active antimicrobial sample was S. adstringens ST3. The extracts of S. adstringens analyzed herein demonstrated not only high content of polyphenols and total tannins, but also antioxidant activity and activity against important bacterial pathogens like Streptococcus pneumoniae, Neisseria gonorrhoeae as well as against multiresistant bacteria such as MRSA (Methicillin-resistant Staphylococcus aureus) and Pseudomonas aeruginosa. The effects of S. adstringens extract on the phagocytosis and intracellular killing of Candida albicans and Candida. kefyr from two normal individuals were studied. We propose that the effect of S. adstringens on the neutrophils may be related to a possible mechanism for regulation functions in these cells.