Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil

Detalhes bibliográficos
Autor(a) principal: Rocha, André Ricardo Ferreira da Silva
Data de Publicação: 2021
Outros Autores: Santos, João Victor da Costa, Moura, Rodrigo Henrique, Lago, Eliana Campelo, Lima, Francisca Lúcia de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/21758
Resumo: Candida albicans is a yeast belonging to the normal microbiota of the human body, considered the most pathogenic species of the genus. It is the main microorganism related to candidiasis. Essential oils of Origanum vulgare have phenolic compounds, such as thymol and carvacrol, which have an effective antimycobacterial character at certain concentrations, but little is known about its biological activity in artisanal preparation. Thus, we evaluated the resistance of standard strains of opportunistic yeast C. albicans against in vitro antifungal activity of artisanal extract of O. vulgare. An inoculum of the challenger was subjected to different concentrations of the fungicidal agent in solidified Muller Hinton and broth followed by incubation at 35°C. The oil was prepared in a manner similar to a possible homemade procedure, and was further sterilized to ensure homogeneity of the indicator. The readings were performed in two days every 24 hours so that there were different possible moments of growth. The tests, which occurred in triplicate, showed that, under experimental conditions, the yeast was resistant to the essential oil compounds at all observed concentrations. Morphological variation was observed in both colonies and yeast cells. Based on analyzes, the artisanal essential oil is incorporated as a promising candidate for the development of antimycotics for clinical use, although in vivo tests are required.
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spelling Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oilProspección de bioactivos anti-Candida en el aceite esencial artesanal de Origanum vulgare L.Prospecção de bioativos anti-Candida no óleo essencial artesanal de Origanum vulgare L.FungicidePlant extractsSecondary CompoundsYeast. FungicidaExtractos vegetalesCompuestos secundariosLevaduras.FungicidaExtratos vegetaisCompostos secundáriosLeveduras.Candida albicans is a yeast belonging to the normal microbiota of the human body, considered the most pathogenic species of the genus. It is the main microorganism related to candidiasis. Essential oils of Origanum vulgare have phenolic compounds, such as thymol and carvacrol, which have an effective antimycobacterial character at certain concentrations, but little is known about its biological activity in artisanal preparation. Thus, we evaluated the resistance of standard strains of opportunistic yeast C. albicans against in vitro antifungal activity of artisanal extract of O. vulgare. An inoculum of the challenger was subjected to different concentrations of the fungicidal agent in solidified Muller Hinton and broth followed by incubation at 35°C. The oil was prepared in a manner similar to a possible homemade procedure, and was further sterilized to ensure homogeneity of the indicator. The readings were performed in two days every 24 hours so that there were different possible moments of growth. The tests, which occurred in triplicate, showed that, under experimental conditions, the yeast was resistant to the essential oil compounds at all observed concentrations. Morphological variation was observed in both colonies and yeast cells. Based on analyzes, the artisanal essential oil is incorporated as a promising candidate for the development of antimycotics for clinical use, although in vivo tests are required.Candida albicans es una levadura perteneciente a la microbiota normal del cuerpo humano, considerada la especie con mayor potencial patógeno del género. Es el principal microorganismo relacionado con la candidiasis. Los aceites esenciales de Origanum vulgare tienen compuestos fenólicos, como el timol y el carvacrol, que extraídos tienen un carácter antimicobacteriano eficaz en determinadas concentraciones, pero se sabe poco sobre su actividad biológica en la preparación artesanal. Por ello, en este trabajo evaluamos la resistencia de cepas estándar de levadura oportunista C. albicans frente a la actividad antifúngica in vitro del aceite esencial artesanal de O. vulgare. Se sometió un inóculo del desafiador a diferentes concentraciones del agente fungicida, en medio Muller Hinton solidificado y en caldo, seguido de incubación a 35ºC. El aceite se preparó de forma similar a un posible procedimiento casero, siendo aún esterilizado para garantizar la homogeneidad del indicador. Las lecturas se tomaron dos días cada 24 horas para que hubiera diferentes tiempos posibles de crecimiento. Las pruebas, que se realizaron por triplicado, demostraron que, en las condiciones experimentales, la levadura demostró ser resistente a los compuestos de aceites esenciales en todas las concentraciones observadas. Se verificó variación morfológica tanto en colonias como en células de levadura. A la vista de los análisis, el aceite esencial artesanal se incorpora como un candidato prometedor para el desarrollo de antimicóticos para uso clínico, que aún requieren pruebas in vivo.Candida albicans é uma levedura pertencente a microbiota normal do corpo humano, considerada, sendo a espécie de maior potencial patogênico no gênero. É o principal microrganismo relacionado a candidíase. Óleos essenciais de Origanum vulgare possuem compostos fenólicos, como timol e carvacrol, que extraídos possuem carácter antimicobacteriano efetivo em certas concentrações, mas pouco se sabe sobre sua atividade biológica em preparo artesanal. Portanto, neste trabalho avaliamos a resistência de linhagens padrão da levedura oportunista C. albicans frente atividade antifúngica in vitro do óleo essencial artesanal de O. vulgare. Um inóculo do desafiante foi submetido a diferentes concentrações do agente fungicida, em meio Muller Hinton solidificado e em caldo, seguido de incubado à 35ºC. O óleo foi preparado de maneira semelhante a um possível procedimento caseiro, sendo ainda esterilizado para garantir a homogeneidade do indicador. As leituras foram realizadas em dois dias à cada 24h para que houvesse diferentes momentos possíveis de crescimento. Os testes, que ocorreram em triplicata, demonstraram que, nas condições de experimentação, a levedura se mostrou resistente aos compostos do óleo essencial em todas as concentrações observadas. Variação morfológica foi verificada tanto nas colônias, quanto nas células das leveduras. Perante análises, incorpora-se o óleo essencial artesanal como um candidato promissor para o desenvolvimento de antimicóticos de uso clínico, ainda sendo necessários testes in vivo.Research, Society and Development2021-10-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2175810.33448/rsd-v10i14.21758Research, Society and Development; Vol. 10 No. 14; e220101421758Research, Society and Development; Vol. 10 Núm. 14; e220101421758Research, Society and Development; v. 10 n. 14; e2201014217582525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/21758/19522Copyright (c) 2021 André Ricardo Ferreira da Silva Rocha; João Victor da Costa Santos; Rodrigo Henrique Moura; Eliana Campelo Lago; Francisca Lúcia de Limahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRocha, André Ricardo Ferreira da SilvaSantos, João Victor da Costa Moura, Rodrigo Henrique Lago, Eliana CampeloLima, Francisca Lúcia de2021-12-04T11:48:39Zoai:ojs.pkp.sfu.ca:article/21758Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:41:06.499564Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil
Prospección de bioactivos anti-Candida en el aceite esencial artesanal de Origanum vulgare L.
Prospecção de bioativos anti-Candida no óleo essencial artesanal de Origanum vulgare L.
title Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil
spellingShingle Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil
Rocha, André Ricardo Ferreira da Silva
Fungicide
Plant extracts
Secondary Compounds
Yeast.
Fungicida
Extractos vegetales
Compuestos secundarios
Levaduras.
Fungicida
Extratos vegetais
Compostos secundários
Leveduras.
title_short Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil
title_full Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil
title_fullStr Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil
title_full_unstemmed Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil
title_sort Prospecting of anti-Candida bioactive in Origanum vulgare L. artisanal essential oil
author Rocha, André Ricardo Ferreira da Silva
author_facet Rocha, André Ricardo Ferreira da Silva
Santos, João Victor da Costa
Moura, Rodrigo Henrique
Lago, Eliana Campelo
Lima, Francisca Lúcia de
author_role author
author2 Santos, João Victor da Costa
Moura, Rodrigo Henrique
Lago, Eliana Campelo
Lima, Francisca Lúcia de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rocha, André Ricardo Ferreira da Silva
Santos, João Victor da Costa
Moura, Rodrigo Henrique
Lago, Eliana Campelo
Lima, Francisca Lúcia de
dc.subject.por.fl_str_mv Fungicide
Plant extracts
Secondary Compounds
Yeast.
Fungicida
Extractos vegetales
Compuestos secundarios
Levaduras.
Fungicida
Extratos vegetais
Compostos secundários
Leveduras.
topic Fungicide
Plant extracts
Secondary Compounds
Yeast.
Fungicida
Extractos vegetales
Compuestos secundarios
Levaduras.
Fungicida
Extratos vegetais
Compostos secundários
Leveduras.
description Candida albicans is a yeast belonging to the normal microbiota of the human body, considered the most pathogenic species of the genus. It is the main microorganism related to candidiasis. Essential oils of Origanum vulgare have phenolic compounds, such as thymol and carvacrol, which have an effective antimycobacterial character at certain concentrations, but little is known about its biological activity in artisanal preparation. Thus, we evaluated the resistance of standard strains of opportunistic yeast C. albicans against in vitro antifungal activity of artisanal extract of O. vulgare. An inoculum of the challenger was subjected to different concentrations of the fungicidal agent in solidified Muller Hinton and broth followed by incubation at 35°C. The oil was prepared in a manner similar to a possible homemade procedure, and was further sterilized to ensure homogeneity of the indicator. The readings were performed in two days every 24 hours so that there were different possible moments of growth. The tests, which occurred in triplicate, showed that, under experimental conditions, the yeast was resistant to the essential oil compounds at all observed concentrations. Morphological variation was observed in both colonies and yeast cells. Based on analyzes, the artisanal essential oil is incorporated as a promising candidate for the development of antimycotics for clinical use, although in vivo tests are required.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/21758
10.33448/rsd-v10i14.21758
url https://rsdjournal.org/index.php/rsd/article/view/21758
identifier_str_mv 10.33448/rsd-v10i14.21758
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/21758/19522
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 14; e220101421758
Research, Society and Development; Vol. 10 Núm. 14; e220101421758
Research, Society and Development; v. 10 n. 14; e220101421758
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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