Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation

Detalhes bibliográficos
Autor(a) principal: Tini, Italo Rigotti Pereira
Data de Publicação: 2021
Outros Autores: Araújo, Juliani Caroline Ribeiro de, Gonçalves, Thais Fernanda, Oliveira, Rogerio de Moraes, Reis, Danieli Aparecida Pereira, Reis, Adriano Gonçalves dos, Vasconcellos, Luana Marotta Reis de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/15644
Resumo: The objectives of this study were to characterize titanium (Ti) surfaces treated by ion implantation by immersion in oxygen plasma (O-PIII) at different temperatures, correlating these implanted layers with therapeutic effects and with osteogenesis, as well as the formation of monotypic biofilms microbial. The groups were divided into: a) Ti (pre-treatment) b) Ti O-PIII at 400 ° C. c) Ti O-PIII at 500 ° C. d) Ti O-PIII at 600 ° C. The properties and surface characteristics were evaluated according to the roughness, texture, corrosion resistance, formation of new phases and the identification of chemical compounds present. Cellular analyzes investigated cell interaction, viability, total protein content, alkaline phosphatase and quantification of mineralized nodules using MG-63 cells. The formation of monotypic microbial biofilms, including P. aeruginosa, S. aureus, S. mutans and C. albicans were evaluated. The increase in surface roughness, corrosion resistance and oxygen content, leading to the formation of TiO2-rutile with more intense peaks and in greater numbers according to the increase in the substrate temperature, ionic implanted Ti samples was observed. There was also a significant increase in cell viability, total protein production, alkaline phosphatase activity and formation of mineralization nodules for the group treated with O-PIII at 600ºC compared to other groups, in addition to a reduction of microorganisms in the groups treated with O- PIII. Therefore, treatment with O-PIII at 600ºC in Ti grade IV showed favorable results for its use.
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spelling Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantationOsteogénesis y formación de biofilms en superficies de titanio sometidas a implantación de iones por inmersión en plasma de oxígenoOsteogênese e formação de biofilmes em superfícies de titânio submetidas à implantação de íons por imersão em plasma de oxigênioOsteogénesisBiocomtibilidadO-PIIIBeiopelículaAleación de titanio.OsteogêneseBiocompatibilidadeO-PIIIBiofilmeLiga de titânio.OsteogenesisBiocompatibilityO-PIIIBiofilmTitanium alloy.The objectives of this study were to characterize titanium (Ti) surfaces treated by ion implantation by immersion in oxygen plasma (O-PIII) at different temperatures, correlating these implanted layers with therapeutic effects and with osteogenesis, as well as the formation of monotypic biofilms microbial. The groups were divided into: a) Ti (pre-treatment) b) Ti O-PIII at 400 ° C. c) Ti O-PIII at 500 ° C. d) Ti O-PIII at 600 ° C. The properties and surface characteristics were evaluated according to the roughness, texture, corrosion resistance, formation of new phases and the identification of chemical compounds present. Cellular analyzes investigated cell interaction, viability, total protein content, alkaline phosphatase and quantification of mineralized nodules using MG-63 cells. The formation of monotypic microbial biofilms, including P. aeruginosa, S. aureus, S. mutans and C. albicans were evaluated. The increase in surface roughness, corrosion resistance and oxygen content, leading to the formation of TiO2-rutile with more intense peaks and in greater numbers according to the increase in the substrate temperature, ionic implanted Ti samples was observed. There was also a significant increase in cell viability, total protein production, alkaline phosphatase activity and formation of mineralization nodules for the group treated with O-PIII at 600ºC compared to other groups, in addition to a reduction of microorganisms in the groups treated with O- PIII. Therefore, treatment with O-PIII at 600ºC in Ti grade IV showed favorable results for its use.Los objetivos de este estudio fueron caracterizar superficies de titanio (Ti) tratadas mediante implantación de iones por inmersión en plasma de oxígeno (O-PIII) a diferentes temperaturas, correlacionando estas capas implantadas con efectos terapéuticos y con osteogénesis, así como la formación de biofilms monotípicos microbianos. Los grupos se dividieron en: a) Ti (pretratamiento) b) Ti O-PIII a 400 ° C c) Ti O-PIII a 500 ° C d) Ti O-PIII a 600 ° C. Las propiedades y se evaluaron las características superficiales según rugosidad, textura, resistencia a la corrosión, formación de nuevas fases e identificación de compuestos químicos presentes. Los análisis celulares investigaron la interacción celular, la viabilidad, el contenido total de proteínas, la fosfatasa alcalina y la cuantificación de nódulos mineralizados utilizando células MG-63. Se evaluó la formación de biofilms microbianos monotípicos, incluidas P. aeruginosa, S. aureus, S. mutans y C. albicans. Se observó el aumento de la rugosidad superficial, la resistencia a la corrosión y el contenido de oxígeno, lo que dio lugar a la formación de TiO2-rutilo con picos más intensos y en mayor número según el aumento de la temperatura del sustrato, se observaron muestras de Ti implantado iónico. También hubo un aumento significativo de la viabilidad celular, producción total de proteínas, actividad de la fosfatasa alcalina y formación de nódulos de mineralización para el grupo tratado con O-PIII a 600 ° C en comparación con otros grupos, además de una reducción de microorganismos en los grupos tratados con O - PIII. Por tanto, el tratamiento con O-PIII a 600ºC em Ti grado IV mostró resultados favorables para su uso.Os objetivos deste estudo foram caracterizar superfícies de titânio (Ti) tratadas por implantação de íons por imersão em plasma de oxigênio (O-PIII) em distintas temperaturas, correlacionando tais camadas implantadas com efeitos terapêuticos e com osteogênese, bem como a formação de biofilmes monotípicos microbianos. Os grupos foram divididos em: a) Ti (pré-tratamento) b) Ti O-PIII a 400 ° C. c) Ti O-PIII a 500 ° C. d) Ti O-PIII a 600 ° C. As propriedades e características superficiais foram avaliadas de acordo com a rugosidade, textura, resistência à corrosão, formação de novas fases e a identificação de compostos químicos presentes. As análises celulares investigaram a interação celular, viabilidade, conteúdo de proteína total, fosfatase alcalina e quantificação de nódulos mineralizados usando células MG-63. A formação de biofilmes microbianos monotípicos, incluindo P. aeruginosa, S. aureus, S. mutans e C. albicans foram avaliadas. O aumento da rugosidade superficial, da resistência à corrosão e do teor de oxigênio, levando à formação de TiO2-rutilo com picos mais intensos e em maior número de acordo com o aumento da temperatura do substrato amostras de Ti implantadas iônicas foi observado. Houve também aumento significativo na viabilidade celular, produção de proteína total, atividade da fosfatase alcalina e formação de nódulos de mineralização para o grupo tratado com O-PIII a 600ºC em comparação com outros grupos, além de redução de microrganismos nos grupos tratados com O-PIII. Portanto, o tratamento com O-PIII a 600ºC em Ti grau IV apresentou resultados favoráveis para sua utilização.Research, Society and Development2021-06-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1564410.33448/rsd-v10i6.15644Research, Society and Development; Vol. 10 No. 6; e37210615644Research, Society and Development; Vol. 10 Núm. 6; e37210615644Research, Society and Development; v. 10 n. 6; e372106156442525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/15644/14213Copyright (c) 2021 Italo Rigotti Pereira Tini; Juliani Caroline Ribeiro de Araújo; Thais Fernanda Gonçalves; Rogerio de Moraes Oliveira; Danieli Aparecida Pereira Reis; Adriano Gonçalves dos Reis; Luana Marotta Reis de Vasconcelloshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTini, Italo Rigotti PereiraAraújo, Juliani Caroline Ribeiro deGonçalves, Thais FernandaOliveira, Rogerio de MoraesReis, Danieli Aparecida PereiraReis, Adriano Gonçalves dosVasconcellos, Luana Marotta Reis de 2021-06-10T22:51:46Zoai:ojs.pkp.sfu.ca:article/15644Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:36:25.263710Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation
Osteogénesis y formación de biofilms en superficies de titanio sometidas a implantación de iones por inmersión en plasma de oxígeno
Osteogênese e formação de biofilmes em superfícies de titânio submetidas à implantação de íons por imersão em plasma de oxigênio
title Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation
spellingShingle Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation
Tini, Italo Rigotti Pereira
Osteogénesis
Biocomtibilidad
O-PIII
Beiopelícula
Aleación de titanio.
Osteogênese
Biocompatibilidade
O-PIII
Biofilme
Liga de titânio.
Osteogenesis
Biocompatibility
O-PIII
Biofilm
Titanium alloy.
title_short Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation
title_full Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation
title_fullStr Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation
title_full_unstemmed Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation
title_sort Osteogenesis and biofilms formation on titanium surfaces submitted to oxygen plasma immersion ion implantation
author Tini, Italo Rigotti Pereira
author_facet Tini, Italo Rigotti Pereira
Araújo, Juliani Caroline Ribeiro de
Gonçalves, Thais Fernanda
Oliveira, Rogerio de Moraes
Reis, Danieli Aparecida Pereira
Reis, Adriano Gonçalves dos
Vasconcellos, Luana Marotta Reis de
author_role author
author2 Araújo, Juliani Caroline Ribeiro de
Gonçalves, Thais Fernanda
Oliveira, Rogerio de Moraes
Reis, Danieli Aparecida Pereira
Reis, Adriano Gonçalves dos
Vasconcellos, Luana Marotta Reis de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tini, Italo Rigotti Pereira
Araújo, Juliani Caroline Ribeiro de
Gonçalves, Thais Fernanda
Oliveira, Rogerio de Moraes
Reis, Danieli Aparecida Pereira
Reis, Adriano Gonçalves dos
Vasconcellos, Luana Marotta Reis de
dc.subject.por.fl_str_mv Osteogénesis
Biocomtibilidad
O-PIII
Beiopelícula
Aleación de titanio.
Osteogênese
Biocompatibilidade
O-PIII
Biofilme
Liga de titânio.
Osteogenesis
Biocompatibility
O-PIII
Biofilm
Titanium alloy.
topic Osteogénesis
Biocomtibilidad
O-PIII
Beiopelícula
Aleación de titanio.
Osteogênese
Biocompatibilidade
O-PIII
Biofilme
Liga de titânio.
Osteogenesis
Biocompatibility
O-PIII
Biofilm
Titanium alloy.
description The objectives of this study were to characterize titanium (Ti) surfaces treated by ion implantation by immersion in oxygen plasma (O-PIII) at different temperatures, correlating these implanted layers with therapeutic effects and with osteogenesis, as well as the formation of monotypic biofilms microbial. The groups were divided into: a) Ti (pre-treatment) b) Ti O-PIII at 400 ° C. c) Ti O-PIII at 500 ° C. d) Ti O-PIII at 600 ° C. The properties and surface characteristics were evaluated according to the roughness, texture, corrosion resistance, formation of new phases and the identification of chemical compounds present. Cellular analyzes investigated cell interaction, viability, total protein content, alkaline phosphatase and quantification of mineralized nodules using MG-63 cells. The formation of monotypic microbial biofilms, including P. aeruginosa, S. aureus, S. mutans and C. albicans were evaluated. The increase in surface roughness, corrosion resistance and oxygen content, leading to the formation of TiO2-rutile with more intense peaks and in greater numbers according to the increase in the substrate temperature, ionic implanted Ti samples was observed. There was also a significant increase in cell viability, total protein production, alkaline phosphatase activity and formation of mineralization nodules for the group treated with O-PIII at 600ºC compared to other groups, in addition to a reduction of microorganisms in the groups treated with O- PIII. Therefore, treatment with O-PIII at 600ºC in Ti grade IV showed favorable results for its use.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/15644
10.33448/rsd-v10i6.15644
url https://rsdjournal.org/index.php/rsd/article/view/15644
identifier_str_mv 10.33448/rsd-v10i6.15644
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/15644/14213
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 6; e37210615644
Research, Society and Development; Vol. 10 Núm. 6; e37210615644
Research, Society and Development; v. 10 n. 6; e37210615644
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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