Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| DOI: | 10.33448/rsd-v12i3.40474 |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/40474 |
Resumo: | Introduction: Mosquitoes of the genus Aedes, especially Aedes aegypti, have a higher prevalence in tropical countries and because they are located in urban environments, they are currently considered the main vectors of arboviruses such as Dengue, Zika, Chikungunya, Yellow Fever, among others. Dengue is one of the most widespread and worrying diseases for public health, as it is characteristic of a seasonal epidemic, and because many insecticides used in smokeless cars are no longer effective against the vector, which makes its control difficult, this study aimed to analyze the genetic profile of Aedes aegypti larvae based on the detection of the mtDNA NADH4 gene, and to analyze possible kdr mutations in the gene that encodes sodium channels related to resistance to insecticides. Materials and method: The kdr mutation research was analyzed based on in the restriction fragment length polymorphism technique (PCR-RFLP) Results: Of the 52 samples analyzed, 24 presented showed polymorphism of bands in the agarose run, confirming the mutation Conclusion: The results obtained in the present study indicated the presence of almost 50% of larvae with mutation for resistance. |
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Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticidesEstudio de la variabilidad genética y análisis de posibles mutaciones kdr en el gen codificador de los canales de sódio relacionados a la resistencia del Aedes aegypti a los insecticidasEstudo da variabilidade genética e análise de possíveis mutações kdr no gene codificador dos canais de sódio relacionados à resistência do Aedes aegypti a inseticidasDengueMosquitoArbovirusPiretroides.DengueMosquitoArbovírusPyrethroids.DengueMosquitoArbovírusPiretróides. Introduction: Mosquitoes of the genus Aedes, especially Aedes aegypti, have a higher prevalence in tropical countries and because they are located in urban environments, they are currently considered the main vectors of arboviruses such as Dengue, Zika, Chikungunya, Yellow Fever, among others. Dengue is one of the most widespread and worrying diseases for public health, as it is characteristic of a seasonal epidemic, and because many insecticides used in smokeless cars are no longer effective against the vector, which makes its control difficult, this study aimed to analyze the genetic profile of Aedes aegypti larvae based on the detection of the mtDNA NADH4 gene, and to analyze possible kdr mutations in the gene that encodes sodium channels related to resistance to insecticides. Materials and method: The kdr mutation research was analyzed based on in the restriction fragment length polymorphism technique (PCR-RFLP) Results: Of the 52 samples analyzed, 24 presented showed polymorphism of bands in the agarose run, confirming the mutation Conclusion: The results obtained in the present study indicated the presence of almost 50% of larvae with mutation for resistance.Introducción: los mosquitos del género Aedes aegypti tienen una mayor predominancia en países tropicales y por estar ubicados en ambientes urbanos, actualmente son considerados los principales vectores de arbovirus como Dengue, Zika, Chikungunya, Fiebre Amarilla, entre otros. El dengue es una de las enfermedades más extendidas y preocupantes para la salud pública, por ser característico de una epidemia estacional, y porque muchos insecticidas utilizados en los automóviles sin humo ya no son efectivos contra el vector, lo que dificulta su control, este estudio tuvo como objetivo analizar el perfil genético de larvas de Aedes aegypti a partir de la detección del gen mtDNA NADH4, y analizar posibles mutaciones kdr en el gen que codifica los canales de sodio relacionadas con la resistencia a insecticidas. Materiales y método: La investigación de mutaciones de kdr se analizó en base a la técnica de polimorfismo de longitud de fragmentos de restricción (PCR-RFLP). Resultados: De las 52 muestras analizadas, 24 mostraron polimorfismo de bandas en la corrida de agarosa, lo que confirma la mutación. Conclusión: Los resultados obtenidos en el presente estudio indicaron la presencia de casi un 50% de larvas con mutación de resistencia.Introdução: Os mosquitos do gênero Aedes, em especial o Aedes aegypti possuem maior prevalência em países tropicais e por estarem localizados em ambientes urbanos, atualmente são considerados os principais vetores de arboviroses como a Dengue, Zika, Chikungunya, Febre Amarela, entre outras. A Dengue é uma das doenças mais abrangente e preocupante para a saúde pública, por ser característica de uma epidemia sazonal, e pelo fato de muitos inseticidas usados em carros fumacê não apresentarem mais eficácia contra o vetor, o que dificulta o seu controle, este estudo teve como objetivo analisar o perfil genético de larvas do Aedes aegypti baseado na detecção do gene mtDNA NADH4, e analisar possíveis mutações kdr no gene que codifica os canais de sódio relacionados à resistência a inseticidas. Materiais e método: A pesquisa da mutação kdr foi analisada baseado na técnica de polimorfismo de comprimento de fragmento de restrição (PCR-RFLP). Resultados: Das 52 amostras analisadas, 24 apresentaram polimorfismo de bandas na corrida em agarose, confirmando a mutação. Conclusão: Os resultados obtidos no presente estudo indicaram a presença de quase 50% de larvas com mutação para resistência.Research, Society and Development2023-03-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/4047410.33448/rsd-v12i3.40474Research, Society and Development; Vol. 12 No. 3; e11312340474Research, Society and Development; Vol. 12 Núm. 3; e11312340474Research, Society and Development; v. 12 n. 3; e113123404742525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/40474/33123Copyright (c) 2023 Andreina Heineck Chiele ; Iara Natália Afonso Florentino; Rafael Giordani Schulz ; Elisson Furlan Figueiredo; Carina Sperotto Librelotto; Jean Colacitehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessChiele , Andreina Heineck Florentino, Iara Natália Afonso Schulz , Rafael Giordani Figueiredo, Elisson Furlan Librelotto, Carina Sperotto Colacite, Jean2023-03-23T08:33:38Zoai:ojs.pkp.sfu.ca:article/40474Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2023-03-23T08:33:38Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides Estudio de la variabilidad genética y análisis de posibles mutaciones kdr en el gen codificador de los canales de sódio relacionados a la resistencia del Aedes aegypti a los insecticidas Estudo da variabilidade genética e análise de possíveis mutações kdr no gene codificador dos canais de sódio relacionados à resistência do Aedes aegypti a inseticidas |
| title |
Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides |
| spellingShingle |
Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides Chiele , Andreina Heineck Dengue Mosquito Arbovirus Piretroides. Dengue Mosquito Arbovírus Pyrethroids. Dengue Mosquito Arbovírus Piretróides. Chiele , Andreina Heineck Dengue Mosquito Arbovirus Piretroides. Dengue Mosquito Arbovírus Pyrethroids. Dengue Mosquito Arbovírus Piretróides. |
| title_short |
Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides |
| title_full |
Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides |
| title_fullStr |
Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides |
| title_full_unstemmed |
Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides |
| title_sort |
Study of genetic variability and analysis of possible kdr mutations in the gene encoding sodium channels related to resistance of Aedes aegypti to insecticides |
| author |
Chiele , Andreina Heineck |
| author_facet |
Chiele , Andreina Heineck Chiele , Andreina Heineck Florentino, Iara Natália Afonso Schulz , Rafael Giordani Figueiredo, Elisson Furlan Librelotto, Carina Sperotto Colacite, Jean Florentino, Iara Natália Afonso Schulz , Rafael Giordani Figueiredo, Elisson Furlan Librelotto, Carina Sperotto Colacite, Jean |
| author_role |
author |
| author2 |
Florentino, Iara Natália Afonso Schulz , Rafael Giordani Figueiredo, Elisson Furlan Librelotto, Carina Sperotto Colacite, Jean |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Chiele , Andreina Heineck Florentino, Iara Natália Afonso Schulz , Rafael Giordani Figueiredo, Elisson Furlan Librelotto, Carina Sperotto Colacite, Jean |
| dc.subject.por.fl_str_mv |
Dengue Mosquito Arbovirus Piretroides. Dengue Mosquito Arbovírus Pyrethroids. Dengue Mosquito Arbovírus Piretróides. |
| topic |
Dengue Mosquito Arbovirus Piretroides. Dengue Mosquito Arbovírus Pyrethroids. Dengue Mosquito Arbovírus Piretróides. |
| description |
Introduction: Mosquitoes of the genus Aedes, especially Aedes aegypti, have a higher prevalence in tropical countries and because they are located in urban environments, they are currently considered the main vectors of arboviruses such as Dengue, Zika, Chikungunya, Yellow Fever, among others. Dengue is one of the most widespread and worrying diseases for public health, as it is characteristic of a seasonal epidemic, and because many insecticides used in smokeless cars are no longer effective against the vector, which makes its control difficult, this study aimed to analyze the genetic profile of Aedes aegypti larvae based on the detection of the mtDNA NADH4 gene, and to analyze possible kdr mutations in the gene that encodes sodium channels related to resistance to insecticides. Materials and method: The kdr mutation research was analyzed based on in the restriction fragment length polymorphism technique (PCR-RFLP) Results: Of the 52 samples analyzed, 24 presented showed polymorphism of bands in the agarose run, confirming the mutation Conclusion: The results obtained in the present study indicated the presence of almost 50% of larvae with mutation for resistance. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-03-02 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/40474 10.33448/rsd-v12i3.40474 |
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https://rsdjournal.org/index.php/rsd/article/view/40474 |
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10.33448/rsd-v12i3.40474 |
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por |
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por |
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https://rsdjournal.org/index.php/rsd/article/view/40474/33123 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 12 No. 3; e11312340474 Research, Society and Development; Vol. 12 Núm. 3; e11312340474 Research, Society and Development; v. 12 n. 3; e11312340474 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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1822182207087706112 |
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10.33448/rsd-v12i3.40474 |