Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/27338 |
Resumo: | The work aims at evaluating the viability of the transient renal blood obliteration method as an alternative to the study of renal CRS in Tayassu tajacu, considering the probable morphological and physiological similarity of this species to humans, since it presents phylogenetic proximity with domestic swine. Eight animals were used, which were submitted to a surgical procedure to induce ischemic nephropathy by clamping the renal vessels with vascular clamp for 60 minutes. Samples were collected for serum urea, creatinine and urinary protein / creatinine ratio (UPC), in addition to renal ultrasonographic evaluation, both before induction and on the 10th and 20th postoperative days, and collection of material for histopathological examination. The results showed a difference in the renal resistivity levels between the evaluated moments, an increase of renal echogenicity with loss of the cortico-spinal relation and presence of discrete glomerular alterations and several tubulointerstitial alterations in the histopathological analysis. It was concluded that the catheter is a good experimental model for studies of induced ischemic nephropathies, but it is necessary to standardize its renal, serological and urinary biochemical parameters in the same nutritional regimen, given the great variability of such data within the species. |
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Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal modelObliteración transitoria de sangre renal como alternativa al estudio del síndrome de isquemia-reperfusión renal en un modelo animalObliteração sanguínea renal transitória como alternativa ao estudo da síndrome de isquemia-reperfusão renal em modelo animalCatetosIsquêmia-reperfusãoModelo animalNefropatias.CathetsNephropathyIschemia-reperfusionAnimal model.Pecaríes de collarNefropatíasIsquemia-reperfusiónModelo de animales.The work aims at evaluating the viability of the transient renal blood obliteration method as an alternative to the study of renal CRS in Tayassu tajacu, considering the probable morphological and physiological similarity of this species to humans, since it presents phylogenetic proximity with domestic swine. Eight animals were used, which were submitted to a surgical procedure to induce ischemic nephropathy by clamping the renal vessels with vascular clamp for 60 minutes. Samples were collected for serum urea, creatinine and urinary protein / creatinine ratio (UPC), in addition to renal ultrasonographic evaluation, both before induction and on the 10th and 20th postoperative days, and collection of material for histopathological examination. The results showed a difference in the renal resistivity levels between the evaluated moments, an increase of renal echogenicity with loss of the cortico-spinal relation and presence of discrete glomerular alterations and several tubulointerstitial alterations in the histopathological analysis. It was concluded that the catheter is a good experimental model for studies of induced ischemic nephropathies, but it is necessary to standardize its renal, serological and urinary biochemical parameters in the same nutritional regimen, given the great variability of such data within the species.Este trabajo tiene como objetivo evaluar la factibilidad del método de obliteración de sangre renal transitoria como una alternativa al estudio de SIR renal en pecaríes de collar (Tayassu tajacu), en vista de la probable similitud morfofisiológica de esta especie con los humanos, ya que presenta proximidad filogenética con el cerdo doméstico. Se utilizaron ocho animales, a los que se les realizó un procedimiento quirúrgico de inducción de nefropatía isquémica pinzando los vasos renales con pinza vascular durante 60 minutos. Fueron colectadas muestras para medición de urea sérica, creatinina y cociente proteína/creatinina urinaria - UPC, además de evaluación ecográfica renal, tanto antes de la inducción como a los 10 y 20 días posinducción, y recolección de material para exámenes histopatológicos. Los resultados mostraron diferencias en los niveles de resistividad renal entre los momentos evaluados, aumento de la ecogenicidad renal con pérdida de la relación córtico-medular y presencia de discretas alteraciones glomerulares y varias alteraciones tubulointersticiales en el análisis histopatológico. Se concluyó que el pecarí de collar se caracteriza como un buen modelo experimental para estudios de nefropatías isquémicas inducidas, sin embargo, es necesario estandarizar sus parámetros bioquímicos renales, serológicos y urinarios en un régimen nutricional idéntico, dada la gran variabilidad de tales datos. dentro de la especie.Este trabalho visa avaliar a viabilidade do método de obliteração sanguínea renal transitória como alternativa ao estudo da SIR renal em catetos (Tayassu tajacu), tendo em vista a provável similaridade morfofisiológica desta espécie com os humanos, uma vez que a mesma apresenta proximidade filogenética com o suíno doméstico. Foram utilizados oito animais, os quais foram submetidos a procedimento cirúrgico para indução de nefropatia isquêmica mediante clampeamento dos vasos renais com pinça vascular por 60 minutos. Foram colhidas amostras para mensuração sérica de ureia, creatinina e relação proteína / creatinina urinária – UPC, além de avaliação ultrassonográfica renal, ambos antes da indução e no 10º e 20º dias pós-indução, e coleta de material para exames histopatológicos. Os resultados demonstraram diferença nos níveis de resistividade renal entre os momentos avaliados, aumento de ecogenicidade renal com perda da relação córtico-medular e presença de discretas alterações glomerulares e várias alterações túbulointersticiais na análise histopatológica. Concluiu-se que o cateto caracteriza-se como um bom modelo experimental para estudos de nefropatias isquêmicas induzidas, contudo faz-se necessário a padronização de seus parâmetros bioquímicos renais, sorológicos e urinários em regime nutricional idêntico, haja visto a grande variabilidade de tais dados dentro da espécie.Research, Society and Development2022-03-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2733810.33448/rsd-v11i4.27338Research, Society and Development; Vol. 11 No. 4; e43911427338Research, Society and Development; Vol. 11 Núm. 4; e43911427338Research, Society and Development; v. 11 n. 4; e439114273382525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/27338/24074Copyright (c) 2022 Robson dos Anjos Honorato; Matheus Levi Tajra Feitosa; Lucilene dos Santos Silva; Maria Acelina Martins de Carvalho; Alcyone de Oliveira Paredes; Nathália Borges de Almeida Angelim; Renato dos Anjos Souzahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessHonorato, Robson dos Anjos Feitosa, Matheus Levi Tajra Silva, Lucilene dos Santos Carvalho, Maria Acelina Martins de Paredes, Alcyone de Oliveira Angelim, Nathália Borges de Almeida Souza, Renato dos Anjos 2022-03-27T17:17:09Zoai:ojs.pkp.sfu.ca:article/27338Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:06.629130Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model Obliteración transitoria de sangre renal como alternativa al estudio del síndrome de isquemia-reperfusión renal en un modelo animal Obliteração sanguínea renal transitória como alternativa ao estudo da síndrome de isquemia-reperfusão renal em modelo animal |
| title |
Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model |
| spellingShingle |
Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model Honorato, Robson dos Anjos Catetos Isquêmia-reperfusão Modelo animal Nefropatias. Cathets Nephropathy Ischemia-reperfusion Animal model. Pecaríes de collar Nefropatías Isquemia-reperfusión Modelo de animales. |
| title_short |
Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model |
| title_full |
Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model |
| title_fullStr |
Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model |
| title_full_unstemmed |
Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model |
| title_sort |
Transient renal blood obliteration as an alternative to the study of renal ischemia-reperfusion syndrome in an animal model |
| author |
Honorato, Robson dos Anjos |
| author_facet |
Honorato, Robson dos Anjos Feitosa, Matheus Levi Tajra Silva, Lucilene dos Santos Carvalho, Maria Acelina Martins de Paredes, Alcyone de Oliveira Angelim, Nathália Borges de Almeida Souza, Renato dos Anjos |
| author_role |
author |
| author2 |
Feitosa, Matheus Levi Tajra Silva, Lucilene dos Santos Carvalho, Maria Acelina Martins de Paredes, Alcyone de Oliveira Angelim, Nathália Borges de Almeida Souza, Renato dos Anjos |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Honorato, Robson dos Anjos Feitosa, Matheus Levi Tajra Silva, Lucilene dos Santos Carvalho, Maria Acelina Martins de Paredes, Alcyone de Oliveira Angelim, Nathália Borges de Almeida Souza, Renato dos Anjos |
| dc.subject.por.fl_str_mv |
Catetos Isquêmia-reperfusão Modelo animal Nefropatias. Cathets Nephropathy Ischemia-reperfusion Animal model. Pecaríes de collar Nefropatías Isquemia-reperfusión Modelo de animales. |
| topic |
Catetos Isquêmia-reperfusão Modelo animal Nefropatias. Cathets Nephropathy Ischemia-reperfusion Animal model. Pecaríes de collar Nefropatías Isquemia-reperfusión Modelo de animales. |
| description |
The work aims at evaluating the viability of the transient renal blood obliteration method as an alternative to the study of renal CRS in Tayassu tajacu, considering the probable morphological and physiological similarity of this species to humans, since it presents phylogenetic proximity with domestic swine. Eight animals were used, which were submitted to a surgical procedure to induce ischemic nephropathy by clamping the renal vessels with vascular clamp for 60 minutes. Samples were collected for serum urea, creatinine and urinary protein / creatinine ratio (UPC), in addition to renal ultrasonographic evaluation, both before induction and on the 10th and 20th postoperative days, and collection of material for histopathological examination. The results showed a difference in the renal resistivity levels between the evaluated moments, an increase of renal echogenicity with loss of the cortico-spinal relation and presence of discrete glomerular alterations and several tubulointerstitial alterations in the histopathological analysis. It was concluded that the catheter is a good experimental model for studies of induced ischemic nephropathies, but it is necessary to standardize its renal, serological and urinary biochemical parameters in the same nutritional regimen, given the great variability of such data within the species. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-03-23 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/27338 10.33448/rsd-v11i4.27338 |
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https://rsdjournal.org/index.php/rsd/article/view/27338 |
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10.33448/rsd-v11i4.27338 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/27338/24074 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
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Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 4; e43911427338 Research, Society and Development; Vol. 11 Núm. 4; e43911427338 Research, Society and Development; v. 11 n. 4; e43911427338 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052707532439552 |