Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/9550 |
Resumo: | Objetive: The present study was to evaluate the acaricidal and larvicidal activity of the essential oil from Psidium rufum leaves against engorged Rhipicephalus microplus females and larvae. Methodology: Essential oil from fresh P. rufum leaves was extracted by hydrodistillation (2h) and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The acaricidal and larvicidal activities were performed using the adult immersion and larval immersion (Larval Paket Test) techniques using the essential oil at concentrations ranging from 300 to 0.001 mg/mL . As positive control was utilized as a commercial solution (15% cypermethrin; 25% chlorpyrifos; 1% citronellal) at 0.005%, and as negative control, an aqueous solution of 2.0% polysorbate (80) was used. Lethal concentrations (LC50 and LC99) were determined by Probit analysis. Results: In the essential oil, 55 compounds were identified, with hydrocarbon sesquiterpenes being the majority class (70.07%), with majority being 1.8-cineole (19.00%); followed by α-longipinene (18.62%). The results showed that the essential oil of P. rufum at 6.20 mg / mL was effective in controlling 73% of the hatchability of eggs, reducing oviposition and the number of adult ticks and larvae. The CL99 for larvae was 2.50 mg / mL, and for engorged females CL99 was 513.57 mg / mL. Conclusion: essential oil from P. rufum leaves demonstrated potential to control R. microplus in the life cycle that corresponds to the larval stage, indicating that it is a promising plant for the development and application of products against bovine ticks. |
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Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves Control de garrapata del ganado bovino (Rhipicephalus microplus) con aceite esencial de hojas de Psidium rufum DCControle de carrapato bovino (Rhipicephalus microplus) com óleo essencial de folhas de Psidium rufum DC1,8-cineoleα-longipineneGuayava cimarrónLarvicidaRhipicephalus microplus.1,8-cineoleα-longipineneAraçá-roxoLarvicidaRhipicephalus microplus.1,8-cineoleα-longipineneAraçá-roxoLarvicideRhipicephalus microplus.Objetive: The present study was to evaluate the acaricidal and larvicidal activity of the essential oil from Psidium rufum leaves against engorged Rhipicephalus microplus females and larvae. Methodology: Essential oil from fresh P. rufum leaves was extracted by hydrodistillation (2h) and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The acaricidal and larvicidal activities were performed using the adult immersion and larval immersion (Larval Paket Test) techniques using the essential oil at concentrations ranging from 300 to 0.001 mg/mL . As positive control was utilized as a commercial solution (15% cypermethrin; 25% chlorpyrifos; 1% citronellal) at 0.005%, and as negative control, an aqueous solution of 2.0% polysorbate (80) was used. Lethal concentrations (LC50 and LC99) were determined by Probit analysis. Results: In the essential oil, 55 compounds were identified, with hydrocarbon sesquiterpenes being the majority class (70.07%), with majority being 1.8-cineole (19.00%); followed by α-longipinene (18.62%). The results showed that the essential oil of P. rufum at 6.20 mg / mL was effective in controlling 73% of the hatchability of eggs, reducing oviposition and the number of adult ticks and larvae. The CL99 for larvae was 2.50 mg / mL, and for engorged females CL99 was 513.57 mg / mL. Conclusion: essential oil from P. rufum leaves demonstrated potential to control R. microplus in the life cycle that corresponds to the larval stage, indicating that it is a promising plant for the development and application of products against bovine ticks.Objetivo: evaluar la actividad acaricida y larvicida del aceite esencial de las hojas de Psidium rufum ante hembras ingurgitadas y larvas de Rhipicephalus microplus. Metodología: el aceite esencial de las hojas frescas de P. rufum fue extraído por hidrodestilación (2h) y analizado por cromatografía gaseosa acoplada a la espectrometría de masa (CG-EM). Las actividades acaricida y larvicida fueron realizadas por la técnica de inmersión de adultos y inmersión larval (Larval packet Test) utilizando el aceite esencial en las concentraciones de 300 a 0,001 mg/mL. Como control positivo fue utilizado una solución comercial (cipermetrina 15%; clorpirifós 25%; citronelal 1%) a 0,005%, y el control negativo una solución acuosa de polisorbato (80) a 2,0%. Las concentraciones letales (CL50 y CL99) fueron determinadas por el análisis de Probitos. Resultados: en el aceite esencial, fueron identificados 55 compuestos, siendo el tipo mayoritario los sesquiterpenos hidrocarbonetos (70,07%), teniendo como mayoritario el 1,8-cineole (19,00%); seguido del α-longipinene (18,62%). Los resultados encontrados mostraron que el aceite esencial de P. rufum a 6.20 mg/mL fue efectivo para controlar 73% de la incubabilidad de huevos con reducción de la capacidad de oviposición y del número de garrapatas adultas y larvas. La actividad larvicida del aceite esencial tuvo CL99 de 2,50 mg/mL, comparado con las hembras ingurgitadas CL99 de 513,57 mg/mL.Conclusión: el aceite esencial de las hojas de P. rufum demostró un potencial para controlar R. microplus en el ciclo de vida que corresponde a la etapa larval, mostrando que se trata de una planta promisora para el desarrollo y aplicación de productos contra garrapatas bovinas.Objetivo: avaliar a atividade acaricida e larvicida do óleo essencial das folhas de Psidium rufum frente a fêmeas ingurgitadas e larvas de Rhipicephalus microplus. Metodologia: o óleo essencial das folhas frescas de P. rufum foi extraído por hidrodestilação (2h) e analisado por cromatografia em fase gasosa acoplada à espectrometria de massas (CG-EM). As atividades acaricida e larvicida foram realizadas pela técnica de imersão de adultos e imersão larval (Larval Paket Test) utilizando o óleo essencial nas concentrações de 300 a 0,001 mg/mL. Como controle positivo foi utilizado uma solução comercial (cipermetrina 15%; clorpirifós 25%; citronelal 1%) a 0,005%, e como controle negativo uma solução aquosa de polissorbato (80) a 2,0%. As concentrações letais (CL50 e CL99) foram determinadas pela análise de Probitos. Resultados: no óleo essencial, foram identificados 55 compostos, sendo a classe majoritária os sesquiterpenos hidrocarbonetos (70,07%), e como majoritários o 1,8-cineole (19,00%); seguido do α-longipinene (18,62%). Os resultados evidenciaram que o óleo essencial de P. rufum a 6.20 mg/mL foi efetivo para controlar 73% da eclodibilidade de ovos reduzindo a oviposição e o número de carrapatos adultos e larvas. A CL99 para as larvas foi 2,50 mg/mL, e para as fêmeas ingurgitadas CL99 de 513,57 mg/mL. Conclusão: o óleo essenciaL das folhas de P. rufum demonstrou potencial para controlar R. microplus no ciclo de vida que corresponde a fase larval, indicando tratar-se de uma planta promissora para o desenvolvimento e aplicação de produtos contra carrapatos bovinos.Research, Society and Development2020-11-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/955010.33448/rsd-v9i11.9550Research, Society and Development; Vol. 9 No. 11; e409119550Research, Society and Development; Vol. 9 Núm. 11; e409119550Research, Society and Development; v. 9 n. 11; e4091195502525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/9550/8508Copyright (c) 2020 Luiz Antônio Branco; Herika Line Marko de Oliveira ; Wanessa de Campos Bortolucci; Carla Maria Mariano Fernandez; José Eduardo Gonçalves; Zilda Cristiani Gazim; Daniela Dib Gonçalves; Emerson Luiz Botelho Lourenço; Jessé Lahos Borges ; Vanderson dos Santos Silva; Ranulfo Piau Juniorhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBranco, Luiz Antônio Oliveira , Herika Line Marko de Bortolucci, Wanessa de Campos Fernandez, Carla Maria MarianoGonçalves, José Eduardo Gazim, Zilda CristianiGonçalves, Daniela Dib Lourenço, Emerson Luiz Botelho Borges , Jessé Lahos Silva, Vanderson dos Santos Piau Junior, Ranulfo2020-12-10T23:37:57Zoai:ojs.pkp.sfu.ca:article/9550Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:48.099745Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves Control de garrapata del ganado bovino (Rhipicephalus microplus) con aceite esencial de hojas de Psidium rufum DC Controle de carrapato bovino (Rhipicephalus microplus) com óleo essencial de folhas de Psidium rufum DC |
title |
Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves |
spellingShingle |
Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves Branco, Luiz Antônio 1,8-cineole α-longipinene Guayava cimarrón Larvicida Rhipicephalus microplus. 1,8-cineole α-longipinene Araçá-roxo Larvicida Rhipicephalus microplus. 1,8-cineole α-longipinene Araçá-roxo Larvicide Rhipicephalus microplus. |
title_short |
Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves |
title_full |
Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves |
title_fullStr |
Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves |
title_full_unstemmed |
Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves |
title_sort |
Control of bovine tick (Rhipicephalus microplus) with essential oil from Psidium rufum DC leaves |
author |
Branco, Luiz Antônio |
author_facet |
Branco, Luiz Antônio Oliveira , Herika Line Marko de Bortolucci, Wanessa de Campos Fernandez, Carla Maria Mariano Gonçalves, José Eduardo Gazim, Zilda Cristiani Gonçalves, Daniela Dib Lourenço, Emerson Luiz Botelho Borges , Jessé Lahos Silva, Vanderson dos Santos Piau Junior, Ranulfo |
author_role |
author |
author2 |
Oliveira , Herika Line Marko de Bortolucci, Wanessa de Campos Fernandez, Carla Maria Mariano Gonçalves, José Eduardo Gazim, Zilda Cristiani Gonçalves, Daniela Dib Lourenço, Emerson Luiz Botelho Borges , Jessé Lahos Silva, Vanderson dos Santos Piau Junior, Ranulfo |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Branco, Luiz Antônio Oliveira , Herika Line Marko de Bortolucci, Wanessa de Campos Fernandez, Carla Maria Mariano Gonçalves, José Eduardo Gazim, Zilda Cristiani Gonçalves, Daniela Dib Lourenço, Emerson Luiz Botelho Borges , Jessé Lahos Silva, Vanderson dos Santos Piau Junior, Ranulfo |
dc.subject.por.fl_str_mv |
1,8-cineole α-longipinene Guayava cimarrón Larvicida Rhipicephalus microplus. 1,8-cineole α-longipinene Araçá-roxo Larvicida Rhipicephalus microplus. 1,8-cineole α-longipinene Araçá-roxo Larvicide Rhipicephalus microplus. |
topic |
1,8-cineole α-longipinene Guayava cimarrón Larvicida Rhipicephalus microplus. 1,8-cineole α-longipinene Araçá-roxo Larvicida Rhipicephalus microplus. 1,8-cineole α-longipinene Araçá-roxo Larvicide Rhipicephalus microplus. |
description |
Objetive: The present study was to evaluate the acaricidal and larvicidal activity of the essential oil from Psidium rufum leaves against engorged Rhipicephalus microplus females and larvae. Methodology: Essential oil from fresh P. rufum leaves was extracted by hydrodistillation (2h) and analyzed by gas chromatography coupled to mass spectrometry (GC-MS). The acaricidal and larvicidal activities were performed using the adult immersion and larval immersion (Larval Paket Test) techniques using the essential oil at concentrations ranging from 300 to 0.001 mg/mL . As positive control was utilized as a commercial solution (15% cypermethrin; 25% chlorpyrifos; 1% citronellal) at 0.005%, and as negative control, an aqueous solution of 2.0% polysorbate (80) was used. Lethal concentrations (LC50 and LC99) were determined by Probit analysis. Results: In the essential oil, 55 compounds were identified, with hydrocarbon sesquiterpenes being the majority class (70.07%), with majority being 1.8-cineole (19.00%); followed by α-longipinene (18.62%). The results showed that the essential oil of P. rufum at 6.20 mg / mL was effective in controlling 73% of the hatchability of eggs, reducing oviposition and the number of adult ticks and larvae. The CL99 for larvae was 2.50 mg / mL, and for engorged females CL99 was 513.57 mg / mL. Conclusion: essential oil from P. rufum leaves demonstrated potential to control R. microplus in the life cycle that corresponds to the larval stage, indicating that it is a promising plant for the development and application of products against bovine ticks. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9550 10.33448/rsd-v9i11.9550 |
url |
https://rsdjournal.org/index.php/rsd/article/view/9550 |
identifier_str_mv |
10.33448/rsd-v9i11.9550 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9550/8508 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 9 No. 11; e409119550 Research, Society and Development; Vol. 9 Núm. 11; e409119550 Research, Society and Development; v. 9 n. 11; e409119550 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052662650241024 |