Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes

Detalhes bibliográficos
Autor(a) principal: Manrique Ayala, Henry Daniel
Data de Publicação: 2021
Outros Autores: Silva Filho, Ednaldo, Souza , Alex Junior Souza de, Rolim Filho , Sebastião Tavares, Garcia, Onel Solano, Vale, William Gomes, Pereira, Washington Luiz Assunção
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/13038
Resumo: The present work aimed to evaluate the macro and microscopic lesions and the distribution of Brucella sp. in the reproductive tract of seropositive female buffaloes to brucellosis. Nineteen genital tracts and eighteen peri-vaginal lymph nodes from previous serum reagent animals to brucella were collected from slaughterhouse, processed and submitted to Fisher's exact test (5%). It was not observed uterine macroscopic lesions from non-pregnant and pregnant until the four month, as well as are infrequent in the peri-vaginal lymph nodes. Microscopical lesions were found as lymphocyte clustering in caruncle in the placentome, necrosis and desquamation of chorionic cells, and in the peri-vaginal lymph nodes may occur oedema, increased neutrophil frequency, lymphocyte depletion, lymphoid hyperplasia, congestion, haemorrhage, and vasculitis. Thus, the IHC displayed great sensitivity to identify the presence of the bacterial antigen in tissues of seropositive animals of this species.
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spelling Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodesHallazgos anatomopatológicos e inmunohistoquímicos de la infección natural por Brucella abortus en útero y ganglios linfáticos peri-vaginales de búfalasAchados anatomopatológicos e imunohistoquímicos da infecção natural por Brucella abortus no útero e linfonodos perivaginais de búfalasBacteriaDiagnósticoEnfermedades infecciosasImpacto en la reproducciónZoonosis.BactériaDiagnósticoDoenças infecciosasImpacto na reproduçãoZoonose.BacteriumDiagnosticsInfection diseasesImpact on reproductionZoonosis.The present work aimed to evaluate the macro and microscopic lesions and the distribution of Brucella sp. in the reproductive tract of seropositive female buffaloes to brucellosis. Nineteen genital tracts and eighteen peri-vaginal lymph nodes from previous serum reagent animals to brucella were collected from slaughterhouse, processed and submitted to Fisher's exact test (5%). It was not observed uterine macroscopic lesions from non-pregnant and pregnant until the four month, as well as are infrequent in the peri-vaginal lymph nodes. Microscopical lesions were found as lymphocyte clustering in caruncle in the placentome, necrosis and desquamation of chorionic cells, and in the peri-vaginal lymph nodes may occur oedema, increased neutrophil frequency, lymphocyte depletion, lymphoid hyperplasia, congestion, haemorrhage, and vasculitis. Thus, the IHC displayed great sensitivity to identify the presence of the bacterial antigen in tissues of seropositive animals of this species.El presente estudio tuvo como objetivo evaluar las lesiones macro y microscópicas y la distribución de Brucella sp. en el tracto reproductivo de búfalas seropositivos para brucelosis. Diecinueve tractos genitales y dieciocho ganglios linfáticos perivaginales de animales que reaccionaron a la serología de brucella fueron recolectados en un matadero, procesados ​​y sometidos a la prueba exacta de Fisher (5%). No hubo lesiones macroscópicas en el útero gestante hasta los cuatro meses de edad y en las no gestantes, así como son infrecuentes en los ganglios linfáticos perivaginales. Se encontraron lesiones microscópicas que contenían grupos de linfocitos en la carúncula en el placentoma, necrosis y descamación de las células coriónicas, y en los ganglios linfáticos perivaginales, edema, aumento de la frecuencia de neutrófilos, depleción de linfocitos, hiperplasia de linfocitos, congestionamiento, hemorrágia y vasculitis. Así, la IHC mostró una gran sensibilidad para identificar la presencia del antígeno bacteriano en tejidos de animales seropositivos de esta especie.O presente trabalho teve como objetivos avaliar as lesões macro e microscópicas e a distribuição de Brucella sp. no trato reprodutivo de búfalas soropositivas para a brucelose. Dezenove tratos genitais e dezoito linfonodos perivaginais de animais reagentes na sorologia à brucela foram coletados em matadouro, sendo realizada ​​a análise macroscópica e histopatológica para a presença de lesões e imunohistoquímica para brucela com os dados - submetidos ao teste exato de Fisher (5%). Não foram observadas lesões macroscópicas nos úteros gravídico até os quatro meses e nos não gravídicos, bem como são infrequentes nos linfonodos perivaginais. Lesões microscópicas foram encontradas contendo aglomerados de linfócitos na carúncula no placentomo, necrose e descamação das células coriônicas, e nos linfonodos perivaginais pode ocorrer edema, aumento da frequência de neutrófilos, depleção de linfócitos, hiperplasia linfóide, congestão, hemorragia e vasculite. Assim, o IHC apresentou grande sensibilidade para identificar a presença do antígeno bacteriano em tecidos de animais soropositivos desta espécie.Research, Society and Development2021-03-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1303810.33448/rsd-v10i3.13038Research, Society and Development; Vol. 10 No. 3; e6210313038Research, Society and Development; Vol. 10 Núm. 3; e6210313038Research, Society and Development; v. 10 n. 3; e62103130382525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/13038/11734Copyright (c) 2021 Henry Daniel Manrique Ayala; Ednaldo Silva Filho; Alex Junior Souza de Souza ; Sebastião Tavares Rolim Filho ; Onel Solano Garcia; William Gomes Vale; Washington Luiz Assunção Pereirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessManrique Ayala, Henry DanielSilva Filho, Ednaldo Souza , Alex Junior Souza de Rolim Filho , Sebastião Tavares Garcia, Onel SolanoVale, William GomesPereira, Washington Luiz Assunção2021-03-28T12:03:35Zoai:ojs.pkp.sfu.ca:article/13038Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:27.225784Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes
Hallazgos anatomopatológicos e inmunohistoquímicos de la infección natural por Brucella abortus en útero y ganglios linfáticos peri-vaginales de búfalas
Achados anatomopatológicos e imunohistoquímicos da infecção natural por Brucella abortus no útero e linfonodos perivaginais de búfalas
title Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes
spellingShingle Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes
Manrique Ayala, Henry Daniel
Bacteria
Diagnóstico
Enfermedades infecciosas
Impacto en la reproducción
Zoonosis.
Bactéria
Diagnóstico
Doenças infecciosas
Impacto na reprodução
Zoonose.
Bacterium
Diagnostics
Infection diseases
Impact on reproduction
Zoonosis.
title_short Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes
title_full Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes
title_fullStr Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes
title_full_unstemmed Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes
title_sort Anatomopathological and immunohistochemical findings of natural Brucella abortus infection in buffalo uterin and peri-vaginal lymph nodes
author Manrique Ayala, Henry Daniel
author_facet Manrique Ayala, Henry Daniel
Silva Filho, Ednaldo
Souza , Alex Junior Souza de
Rolim Filho , Sebastião Tavares
Garcia, Onel Solano
Vale, William Gomes
Pereira, Washington Luiz Assunção
author_role author
author2 Silva Filho, Ednaldo
Souza , Alex Junior Souza de
Rolim Filho , Sebastião Tavares
Garcia, Onel Solano
Vale, William Gomes
Pereira, Washington Luiz Assunção
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Manrique Ayala, Henry Daniel
Silva Filho, Ednaldo
Souza , Alex Junior Souza de
Rolim Filho , Sebastião Tavares
Garcia, Onel Solano
Vale, William Gomes
Pereira, Washington Luiz Assunção
dc.subject.por.fl_str_mv Bacteria
Diagnóstico
Enfermedades infecciosas
Impacto en la reproducción
Zoonosis.
Bactéria
Diagnóstico
Doenças infecciosas
Impacto na reprodução
Zoonose.
Bacterium
Diagnostics
Infection diseases
Impact on reproduction
Zoonosis.
topic Bacteria
Diagnóstico
Enfermedades infecciosas
Impacto en la reproducción
Zoonosis.
Bactéria
Diagnóstico
Doenças infecciosas
Impacto na reprodução
Zoonose.
Bacterium
Diagnostics
Infection diseases
Impact on reproduction
Zoonosis.
description The present work aimed to evaluate the macro and microscopic lesions and the distribution of Brucella sp. in the reproductive tract of seropositive female buffaloes to brucellosis. Nineteen genital tracts and eighteen peri-vaginal lymph nodes from previous serum reagent animals to brucella were collected from slaughterhouse, processed and submitted to Fisher's exact test (5%). It was not observed uterine macroscopic lesions from non-pregnant and pregnant until the four month, as well as are infrequent in the peri-vaginal lymph nodes. Microscopical lesions were found as lymphocyte clustering in caruncle in the placentome, necrosis and desquamation of chorionic cells, and in the peri-vaginal lymph nodes may occur oedema, increased neutrophil frequency, lymphocyte depletion, lymphoid hyperplasia, congestion, haemorrhage, and vasculitis. Thus, the IHC displayed great sensitivity to identify the presence of the bacterial antigen in tissues of seropositive animals of this species.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/13038
10.33448/rsd-v10i3.13038
url https://rsdjournal.org/index.php/rsd/article/view/13038
identifier_str_mv 10.33448/rsd-v10i3.13038
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/13038/11734
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 3; e6210313038
Research, Society and Development; Vol. 10 Núm. 3; e6210313038
Research, Society and Development; v. 10 n. 3; e6210313038
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052805779816448

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