Bacteriotech construction in a higher education institution for teaching and research purposes

• Autor(a) principal: Amorim, Carolina Ferreira
• Data de Publicação: 2020
• Outros Autores: Oliveira, Ana Carolina Santana de, Costa , Emanuela Avelar Silva
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Research, Society and Development
• Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/7101
• topic: Bacterioteca; Criopreservação; Ensino; Pesquisa.; Bacteriotech; Cryopreservation; Teaching; Research.; Bacteriotech; Criopreservación; Enseñanza; Investigación.

Introduction: Bacteriotecs have one for great relevance to Higher Education Institutions, both for didactic use and for use in research. The storage methods aim to preserve the morphophysiological aspects of the specimens for use at any time. Microbial conservation techniques can be short term, medium term and long term. Among the principles of each technique are cryopreservation methods with common freezing and deep-freezing associated with cryoprotective additives to reduce and protect freeze-related damage. Over the years, the construction of the bacteotecs has been consolidated by providing bacterial bioavailability for educational and research purposes. Objective: This research aimed at building the bacteriotech for the Microbiology Laboratory of the Higher Education Unit of Feira de Santana. Methodology: 14 bacteria were introduced during the research, applying the storage by freezing at -20° C with 20% glycerol and 15% skimmed milk in TSB broth, in addition to the management for maintenance, feasibility and quality control performed in parallel. Results: The technique adopted for cryopreservation in the medium term with the use of two cryoprotectors, as well as the procedures adopted for viability, were able to guarantee for 1 year and 4 months the purity of the available strains without loss of viability. Conclusion: It was noted that the insertion of the bacteriotech was able to meet the academic and scientific needs of the Institution, so the bacteriotech can be inserted with easy and low-cost techniques.