Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/17783 |
Resumo: | The aim of this study was to investigate the production of anti-Listeria bacteriocins by naturally occurring bacteria on silage and to characterize the most promising bacteriocin. The production of bacteriocins was evaluated by the spot-on-lawn method. The presence of interfering factors and the sensitivity to proteinase K were analyzed. The spectrum of activity was determined and the most promising bacteriocin-producing isolate was identified, being selected for the subsequent experiments. The antimicrobial peptide was extracted, analyzed regarding temperature and pH sensitivities, and purified. Of the 37 isolates evaluated, seven showed anti-Listeria activity. The presence of bacteriophages and production of acidic compounds were not detected. The selected antimicrobial compound was sensitive to proteinase K and the producing-bacterium was identified as Bacillus velezensis. The crude extract maintained the antimicrobial activity in different temperatures and pH conditions. In conclusion, the bacteriocin produced by Bacillus velezensis showed strong activity against Listeria monocytogenes, and great stability to elevated temperature and adverse pH, desirable features for future biotechnological applications. |
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Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silageInhibición de Listeria monocytogenes por Bacillus velezensis el productor de bacteriocina aislado del ensilajeInibição de Listeria monocytogenes por Bacillus velezensis produtor de bacteriocina isolado de silagemAtividade anti-ListeriaBacteriocinaBacillus.Actividad anti-ListeriaBacteriocinaBacillus.Anti-Listeria activityBacteriocinBacillus. The aim of this study was to investigate the production of anti-Listeria bacteriocins by naturally occurring bacteria on silage and to characterize the most promising bacteriocin. The production of bacteriocins was evaluated by the spot-on-lawn method. The presence of interfering factors and the sensitivity to proteinase K were analyzed. The spectrum of activity was determined and the most promising bacteriocin-producing isolate was identified, being selected for the subsequent experiments. The antimicrobial peptide was extracted, analyzed regarding temperature and pH sensitivities, and purified. Of the 37 isolates evaluated, seven showed anti-Listeria activity. The presence of bacteriophages and production of acidic compounds were not detected. The selected antimicrobial compound was sensitive to proteinase K and the producing-bacterium was identified as Bacillus velezensis. The crude extract maintained the antimicrobial activity in different temperatures and pH conditions. In conclusion, the bacteriocin produced by Bacillus velezensis showed strong activity against Listeria monocytogenes, and great stability to elevated temperature and adverse pH, desirable features for future biotechnological applications.El objetivo de este trabajo fue investigar la producción de bacteriocinas con actividad anti-Listeria por bacterias presentes de forma natural en el ensilaje y caracterizar la bacteriocina más prometedora. La producción de bacteriocinas se evaluó mediante el método de superposición. Se analizó la presencia de factores interferentes y la sensibilidad a proteinasa K. Se determinó el espectro de actividad y se identificó el aislado productor de bacteriocina más prometedor, siendo seleccionado para experimentos posteriores. Se extrajo el péptido antimicrobiano, se analizó la sensibilidad a la temperatura y al pH y se purificó. De los 37 aislamientos evaluados, siete mostraron actividad anti-Listeria. No se detectó presencia de bacteriófagos ni producción de compuestos ácidos. El compuesto antimicrobiano seleccionado era sensible a la proteinasa K y la bacteria productora se identificó como Bacillus velezensis. El extracto crudo mantuvo su actividad antimicrobiana a diferentes temperaturas y condiciones de pH. En conclusión, la bacteriocina producida por Bacillus velezensis mostró alta actividad contra Listeria monocytogenes, y gran estabilidad a alta temperatura y pH adverso, características deseables para futuras aplicaciones biotecnológicas.O objetivo deste trabalho foi investigar a produção de bacteriocinas com atividade anti-Listeria por bactérias naturalmente presentes na silagem e caracterizar a bacteriocina mais promissora. A produção de bacteriocinas foi avaliada pelo método de sobrecamada. A presença de fatores interferentes e a sensibilidade à proteinase K foram analisadas. O espectro de atividade foi determinado e o isolado produtor de bacteriocina mais promissor foi identificado, sendo selecionado para os experimentos subsequentes. O peptídeo antimicrobiano foi extraído, analisado quanto à sensibilidade à temperatura e ao pH, e purificado. Dos 37 isolados avaliados, sete apresentaram atividade anti-Listeria. Não foi detectada a presença de bacteriófagos e produção de compostos ácidos. O composto antimicrobiano selecionado foi sensível à proteinase K e a bactéria produtora foi identificada como Bacillus velezensis. O extrato bruto manteve a atividade antimicrobiana em diferentes temperaturas e condições de pH. Em conclusão, a bacteriocina produzida por Bacillus velezensis apresentou elevada atividade contra Listeria monocytogenes, e grande estabilidade a temperatura elevada e pH adverso, características desejáveis para futuras aplicações biotecnológicas.Research, Society and Development2021-07-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1778310.33448/rsd-v10i9.17783Research, Society and Development; Vol. 10 No. 9; e2610917783Research, Society and Development; Vol. 10 Núm. 9; e2610917783Research, Society and Development; v. 10 n. 9; e26109177832525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/17783/15863Copyright (c) 2021 Laura Bonato Alves Oliveira; Yasmin Neves Vieira Sabino; Marlon do Valle Barroso; Ranaíla Kely Ferreira; Junior Fernandes Lima; Pedro Braga Arcuri; Jailton da Costa Carneiro; Ricardo José de Mendonça; João Batista Ribeiro; Alessandra Barbosa Ferreira-Machado; Aline Dias Paivahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Laura Bonato AlvesSabino, Yasmin Neves Vieira Barroso, Marlon do Valle Ferreira, Ranaíla Kely Lima, Junior FernandesArcuri, Pedro BragaCarneiro, Jailton da CostaMendonça, Ricardo José deRibeiro, João BatistaFerreira-Machado, Alessandra Barbosa Paiva, Aline Dias2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/17783Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:03.792379Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage Inhibición de Listeria monocytogenes por Bacillus velezensis el productor de bacteriocina aislado del ensilaje Inibição de Listeria monocytogenes por Bacillus velezensis produtor de bacteriocina isolado de silagem |
| title |
Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage |
| spellingShingle |
Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage Oliveira, Laura Bonato Alves Atividade anti-Listeria Bacteriocina Bacillus. Actividad anti-Listeria Bacteriocina Bacillus. Anti-Listeria activity Bacteriocin Bacillus. |
| title_short |
Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage |
| title_full |
Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage |
| title_fullStr |
Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage |
| title_full_unstemmed |
Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage |
| title_sort |
Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage |
| author |
Oliveira, Laura Bonato Alves |
| author_facet |
Oliveira, Laura Bonato Alves Sabino, Yasmin Neves Vieira Barroso, Marlon do Valle Ferreira, Ranaíla Kely Lima, Junior Fernandes Arcuri, Pedro Braga Carneiro, Jailton da Costa Mendonça, Ricardo José de Ribeiro, João Batista Ferreira-Machado, Alessandra Barbosa Paiva, Aline Dias |
| author_role |
author |
| author2 |
Sabino, Yasmin Neves Vieira Barroso, Marlon do Valle Ferreira, Ranaíla Kely Lima, Junior Fernandes Arcuri, Pedro Braga Carneiro, Jailton da Costa Mendonça, Ricardo José de Ribeiro, João Batista Ferreira-Machado, Alessandra Barbosa Paiva, Aline Dias |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Laura Bonato Alves Sabino, Yasmin Neves Vieira Barroso, Marlon do Valle Ferreira, Ranaíla Kely Lima, Junior Fernandes Arcuri, Pedro Braga Carneiro, Jailton da Costa Mendonça, Ricardo José de Ribeiro, João Batista Ferreira-Machado, Alessandra Barbosa Paiva, Aline Dias |
| dc.subject.por.fl_str_mv |
Atividade anti-Listeria Bacteriocina Bacillus. Actividad anti-Listeria Bacteriocina Bacillus. Anti-Listeria activity Bacteriocin Bacillus. |
| topic |
Atividade anti-Listeria Bacteriocina Bacillus. Actividad anti-Listeria Bacteriocina Bacillus. Anti-Listeria activity Bacteriocin Bacillus. |
| description |
The aim of this study was to investigate the production of anti-Listeria bacteriocins by naturally occurring bacteria on silage and to characterize the most promising bacteriocin. The production of bacteriocins was evaluated by the spot-on-lawn method. The presence of interfering factors and the sensitivity to proteinase K were analyzed. The spectrum of activity was determined and the most promising bacteriocin-producing isolate was identified, being selected for the subsequent experiments. The antimicrobial peptide was extracted, analyzed regarding temperature and pH sensitivities, and purified. Of the 37 isolates evaluated, seven showed anti-Listeria activity. The presence of bacteriophages and production of acidic compounds were not detected. The selected antimicrobial compound was sensitive to proteinase K and the producing-bacterium was identified as Bacillus velezensis. The crude extract maintained the antimicrobial activity in different temperatures and pH conditions. In conclusion, the bacteriocin produced by Bacillus velezensis showed strong activity against Listeria monocytogenes, and great stability to elevated temperature and adverse pH, desirable features for future biotechnological applications. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-07-20 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/17783 10.33448/rsd-v10i9.17783 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/17783 |
| identifier_str_mv |
10.33448/rsd-v10i9.17783 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://rsdjournal.org/index.php/rsd/article/view/17783/15863 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
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Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 9; e2610917783 Research, Society and Development; Vol. 10 Núm. 9; e2610917783 Research, Society and Development; v. 10 n. 9; e2610917783 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052683261050880 |