Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/13842 |
Resumo: | Exfoliative cytology screening for genotoxicity is an important strategy for mitigating tobacco-induced carcinogenesis. The aim of this study was to determine the frequency of micronuclei in oral epithelial cells of smoking and non-smoking patients. Sixteen volunteers, of both sexes, 40-60 years old, with healthy oral mucosa were divided into smoking or non-smoking group. Cytological smears of buccal mucosa, lateral border of the tongue and floor of the mouth stained by Pap stain allowed evaluation of 4800 microscopic images. Frequencies of micronucleated cells (MNC) and micronuclei/cell (MN/C) were compared by group, anatomical region, sex and age range, using the two-way ANOVA test followed by Tukey (p<0.05). There was a greater presence of MNC in the smoker than in the non-smoker group, with significant differences for floor of mouth, female gender and age range of 50-60 years. The amount of 1 or 2 MN/C was greater than 3 or ≥4 MN/C, especially in the smoking group. There were differences in the frequency of MN/C between smokers and non-smokers, for the three oral regions, for both sexes although more accentuated in women, and for the age range of 50-60 years beyond the age range of 40-50 years for the non-smoking group. Differences in MNC and MN/C according to oral regions, sex and age between smokers and non-smokers contribute to understand the genotoxicity mediated by tobacco. |
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Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patientsEvaluación citomorfométrica de micronúcleos en células epiteliales orales de pacientes fumadores y no fumadores Avaliação citomorfométrica de micronúcleos de células epiteliais orais de pacientes fumantes e não fumantesMicronúcleos com defeito cromossômicoPatologiaMucosa bucalTabagismo.Micronúcleos con Defecto CromosómicoPatologíaMucosa bucalTabaquismo.Micronuclei chromossome-defectivePathologyOral mucosaTobacco use disorder.Exfoliative cytology screening for genotoxicity is an important strategy for mitigating tobacco-induced carcinogenesis. The aim of this study was to determine the frequency of micronuclei in oral epithelial cells of smoking and non-smoking patients. Sixteen volunteers, of both sexes, 40-60 years old, with healthy oral mucosa were divided into smoking or non-smoking group. Cytological smears of buccal mucosa, lateral border of the tongue and floor of the mouth stained by Pap stain allowed evaluation of 4800 microscopic images. Frequencies of micronucleated cells (MNC) and micronuclei/cell (MN/C) were compared by group, anatomical region, sex and age range, using the two-way ANOVA test followed by Tukey (p<0.05). There was a greater presence of MNC in the smoker than in the non-smoker group, with significant differences for floor of mouth, female gender and age range of 50-60 years. The amount of 1 or 2 MN/C was greater than 3 or ≥4 MN/C, especially in the smoking group. There were differences in the frequency of MN/C between smokers and non-smokers, for the three oral regions, for both sexes although more accentuated in women, and for the age range of 50-60 years beyond the age range of 40-50 years for the non-smoking group. Differences in MNC and MN/C according to oral regions, sex and age between smokers and non-smokers contribute to understand the genotoxicity mediated by tobacco.El cribado mediante citología exfoliativa para la genotoxicidad es una estrategia importante para mitigar la carcinogénesis oral inducida por el tabaco. El objetivo de este estudio fue determinar la frecuencia de micronúcleos en las células epiteliales orales de pacientes fumadores y no fumadores. Dieciséis voluntarios, de ambos sexos, de 40 a 60 años, con mucosa oral sana, se dividieron en grupos de fumadores y no fumadores. Los frotis citológicos de la mucosa de la mejilla, el borde lateral de la lengua y el piso de la boca teñidos por Papanicolaou permitieron la evaluación de 4800 imágenes microscópicas. Se compararon las frecuencias de células micronucleadas (CMN) y micronúcleos/célula (MN/C) por grupo, región anatómica, sexo y edad, utilizando la prueba ANOVA bidireccional seguida de Tukey (p <0,05). Hubo mayor presencia de CMN en el grupo fumador que en el grupo no fumador, con diferencias significativas para piso de boca, sexo femenino y rango de 50-60 años. La cantidad de 1 o 2 MN/C fue mayor que 3 o ≥4 MN/C, especialmente en el grupo de fumadores. Hubo diferencias en la frecuencia de MN/C entre fumadores y no fumadores, para las tres regiones bucales, para ambos sexos, aunque más acentuadas en las mujeres, y para el rango de 50-60 años, además del rango de 40-50 años para el grupo de no fumadores. Las diferencias en CMN y MN/C según regiones orales, sexo y edad entre fumadores y no fumadores contribuyen a comprender la genotoxicidad mediada por el tabaco.O rastreamento por citologia esfoliativa de genotoxicidade é uma estratégia importante para mitigação da carcinogênese bucal induzida pelo tabaco. O objetivo deste estudo foi determinar a frequência de micronúcleos em células epiteliais orais de pacientes fumantes e não fumantes. Dezesseis voluntários, de ambos os sexos, 40-60 anos, com mucosa bucal saudável, foram divididos em grupo fumante ou não fumante. Esfregaços citológicos de mucosa jugal, borda lateral da língua e assoalho da boca corados por Papanicolaou permitiram avaliação de 4800 imagens microscópicas. Frequências de células micronucleadas (CMN) e micronúcleos/célula (MN/C) foram comparadas por grupo, região anatômica, sexo e idade, usando o teste two-way ANOVA seguido por Tukey (p<0,05). Houve maior presença de CMN no grupo fumante do que não fumante, com diferenças significativas para assoalho de boca, sexo feminino e faixa de 50-60 anos. A quantidade de 1 ou 2 MN/C foi maior que 3 ou ≥4 MN/C, em especial no grupo fumante. Houve diferenças na frequência de MN/C entre fumantes e não fumantes, para as três regiões bucais, para ambos os sexos, embora de forma mais acentuada em mulheres, e para a faixa de 50-60 anos, além da faixa de 40-50 anos para o grupo não fumante. Diferenças de CMN e MN/C segundo regiões bucais, sexo e idade entre fumantes e não fumantes contribuem para compreender a genotoxicidade mediada pelo tabaco.Research, Society and Development2021-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1384210.33448/rsd-v10i4.13842Research, Society and Development; Vol. 10 No. 4; e8810413842Research, Society and Development; Vol. 10 Núm. 4; e8810413842Research, Society and Development; v. 10 n. 4; e88104138422525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/13842/12450Copyright (c) 2021 Haydee Gina Quispe Jiménez; Rômulo Melo Mesquita; Victor Bento Oliveira; Ana Larissa de Queiroz França; Marcelo Miranda de Melo; Ana Liza Paz Sousa Batista; Igor Iuco Castro-Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessQuispe Jiménez, Haydee Gina Mesquita, Rômulo Melo Oliveira, Victor Bento França, Ana Larissa de Queiroz Melo, Marcelo Miranda de Batista, Ana Liza Paz Sousa Castro-Silva, Igor Iuco2021-04-25T11:21:26Zoai:ojs.pkp.sfu.ca:article/13842Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:05.960850Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients Evaluación citomorfométrica de micronúcleos en células epiteliales orales de pacientes fumadores y no fumadores Avaliação citomorfométrica de micronúcleos de células epiteliais orais de pacientes fumantes e não fumantes |
title |
Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients |
spellingShingle |
Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients Quispe Jiménez, Haydee Gina Micronúcleos com defeito cromossômico Patologia Mucosa bucal Tabagismo. Micronúcleos con Defecto Cromosómico Patología Mucosa bucal Tabaquismo. Micronuclei chromossome-defective Pathology Oral mucosa Tobacco use disorder. |
title_short |
Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients |
title_full |
Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients |
title_fullStr |
Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients |
title_full_unstemmed |
Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients |
title_sort |
Cytomorphometric evaluation of micronuclei in oral epithelial cells of smoker and non-smoker patients |
author |
Quispe Jiménez, Haydee Gina |
author_facet |
Quispe Jiménez, Haydee Gina Mesquita, Rômulo Melo Oliveira, Victor Bento França, Ana Larissa de Queiroz Melo, Marcelo Miranda de Batista, Ana Liza Paz Sousa Castro-Silva, Igor Iuco |
author_role |
author |
author2 |
Mesquita, Rômulo Melo Oliveira, Victor Bento França, Ana Larissa de Queiroz Melo, Marcelo Miranda de Batista, Ana Liza Paz Sousa Castro-Silva, Igor Iuco |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Quispe Jiménez, Haydee Gina Mesquita, Rômulo Melo Oliveira, Victor Bento França, Ana Larissa de Queiroz Melo, Marcelo Miranda de Batista, Ana Liza Paz Sousa Castro-Silva, Igor Iuco |
dc.subject.por.fl_str_mv |
Micronúcleos com defeito cromossômico Patologia Mucosa bucal Tabagismo. Micronúcleos con Defecto Cromosómico Patología Mucosa bucal Tabaquismo. Micronuclei chromossome-defective Pathology Oral mucosa Tobacco use disorder. |
topic |
Micronúcleos com defeito cromossômico Patologia Mucosa bucal Tabagismo. Micronúcleos con Defecto Cromosómico Patología Mucosa bucal Tabaquismo. Micronuclei chromossome-defective Pathology Oral mucosa Tobacco use disorder. |
description |
Exfoliative cytology screening for genotoxicity is an important strategy for mitigating tobacco-induced carcinogenesis. The aim of this study was to determine the frequency of micronuclei in oral epithelial cells of smoking and non-smoking patients. Sixteen volunteers, of both sexes, 40-60 years old, with healthy oral mucosa were divided into smoking or non-smoking group. Cytological smears of buccal mucosa, lateral border of the tongue and floor of the mouth stained by Pap stain allowed evaluation of 4800 microscopic images. Frequencies of micronucleated cells (MNC) and micronuclei/cell (MN/C) were compared by group, anatomical region, sex and age range, using the two-way ANOVA test followed by Tukey (p<0.05). There was a greater presence of MNC in the smoker than in the non-smoker group, with significant differences for floor of mouth, female gender and age range of 50-60 years. The amount of 1 or 2 MN/C was greater than 3 or ≥4 MN/C, especially in the smoking group. There were differences in the frequency of MN/C between smokers and non-smokers, for the three oral regions, for both sexes although more accentuated in women, and for the age range of 50-60 years beyond the age range of 40-50 years for the non-smoking group. Differences in MNC and MN/C according to oral regions, sex and age between smokers and non-smokers contribute to understand the genotoxicity mediated by tobacco. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13842 10.33448/rsd-v10i4.13842 |
url |
https://rsdjournal.org/index.php/rsd/article/view/13842 |
identifier_str_mv |
10.33448/rsd-v10i4.13842 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13842/12450 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 4; e8810413842 Research, Society and Development; Vol. 10 Núm. 4; e8810413842 Research, Society and Development; v. 10 n. 4; e8810413842 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052673472593920 |