Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/20923 |
Resumo: | Clinostomum complanatum Rudolphi, 1814 is a digenetic trematode that infects mollusks, several species of freshwater fish and piscivorous birds. In its life cycle, C. complanatum has as its second intermediate host some freshwater fish, among which Jundiá (Rhamdia quelen). Thus, the aim of the present study was to evaluate the prevalence of Clinostomum infections in Jundiás captured in the estuary region of Pelotas/RS and to standardize a Polymerase Chain Reaction (PCR) to diagnose the presence of metacercariae in tissue samples from fish captured in this estuary. In the cross-sectional study and the standardization of PCR, 174 samples of Jundiás (Rhamdia quelen), captured by amateur fishermen at 5 different points in locations close to Laguna dos Patos, Lagoa Mirim or the São Gonçalo Channel were used. The prevalence of parasitism by Clinostomum spp. with diagnosis through parasitological keys was 36.21%, with variations in prevalence according to the reported capture points of specimens from Jundiá, being confirmed by standardized PCR. It is concluded that the prevalence is high in the analyzed silver catfish and PCR can be an auxiliary tool in the diagnosis of this parasite, increasing not only the sensitivity, but also the speed of the test. Such factors will be of great importance for future epidemiological studies, as well as for public health, since the consumption of freshwater fish, typical of oriental cuisine, is expanding in Rio Grande do Sul and Brazil. |
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Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCRPrevalencia de trematodos del género Clinostomum en Jundiás (Rhamdia quelen) y desarrollo de un método diagnóstico mediante PCRPrevalência de trematódeos do gênero Clinostomum em Jundiás (Rhamdia quelen) e desenvolvimento de um método de diagnóstico através de PCRParasitismPublic healthMolecular diagnosisFish.ParasitismoSaúde públicaDiagnóstico molecularPeixes.ParasitismoSalud públicaDiagnóstico molecularPeces.Clinostomum complanatum Rudolphi, 1814 is a digenetic trematode that infects mollusks, several species of freshwater fish and piscivorous birds. In its life cycle, C. complanatum has as its second intermediate host some freshwater fish, among which Jundiá (Rhamdia quelen). Thus, the aim of the present study was to evaluate the prevalence of Clinostomum infections in Jundiás captured in the estuary region of Pelotas/RS and to standardize a Polymerase Chain Reaction (PCR) to diagnose the presence of metacercariae in tissue samples from fish captured in this estuary. In the cross-sectional study and the standardization of PCR, 174 samples of Jundiás (Rhamdia quelen), captured by amateur fishermen at 5 different points in locations close to Laguna dos Patos, Lagoa Mirim or the São Gonçalo Channel were used. The prevalence of parasitism by Clinostomum spp. with diagnosis through parasitological keys was 36.21%, with variations in prevalence according to the reported capture points of specimens from Jundiá, being confirmed by standardized PCR. It is concluded that the prevalence is high in the analyzed silver catfish and PCR can be an auxiliary tool in the diagnosis of this parasite, increasing not only the sensitivity, but also the speed of the test. Such factors will be of great importance for future epidemiological studies, as well as for public health, since the consumption of freshwater fish, typical of oriental cuisine, is expanding in Rio Grande do Sul and Brazil.Clinostomum complanatum Rudolphi, 1814 es un trematodo digenetic que infecta a los moluscos, varias especies de peces de agua dulce y aves piscívoras. En su ciclo de vida, C. complanatum tiene como segundo hospedador intermedio algunos peces de agua dulce, entre los que se encuentran Jundiá (Rhamdia quelen). Así, el objetivo del presente estudio fue evaluar la prevalencia de infecciones por Clinostomum en Jundiás capturadas en la región del estuario de Pelotas/RS y estandarizar la Reacción en Cadena de la Polimerasa (PCR) para diagnosticar la presencia de metacercarias en muestras de tejido de peces capturados en este estuario. Para realizar el estudio transversal y estandarización de la PCR, se utilizaron 174 muestras de Jundiás (Rhamdia quelen), capturadas por pescadores aficionados en 5 puntos diferentes en localidades cercanas a Laguna dos Patos, Lagoa Mirim o Canal São Gonçalo. La prevalencia de parasitismo por Clinostomum spp. con diagnóstico por claves parasitológicas fue de 36,21%, con variaciones en la prevalencia según los puntos de captura reportados de los especímenes de Jundiá, siendo confirmada por PCR estandarizada. Se concluye que la prevalencia es alta en el bagre plateado analizado y la PCR puede ser una herramienta auxiliar en el diagnóstico de este parásito, aumentando no solo la sensibilidad, sino también la rapidez de la prueba. Tales factores serán de gran importancia para futuros estudios epidemiológicos, así como para la salud pública, ya que el consumo de pescado de agua dulce, típico de la cocina oriental, se está expandiendo en Rio Grande do Sul y Brasil.Clinostomum complanatum Rudolphi, 1814 é um trematódeo digenético que infecta moluscos, diversas espécies de peixes de água doce e pássaros piscívoros. Em seu ciclo de vida, o C. complanatum possui como segundo hospedeiro intermediário alguns peixes de água doce, dentre os quais o Jundiá (Rhamdia quelen). Desse modo, o objetivo do presente estudo foi avaliar a prevalência de infecções por Clinostomum em Jundiás capturados na região estuária de Pelotas/RS e padronizar a Reação em Cadeia da Polimerase (PCR) para diagnóstico da presença de metacercárias em amostras de tecidos de peixes capturados neste estuário. Para a realização do estudo transversal e padronização da PCR, utilizou-se 174 amostras de Jundiás (Rhamdia quelen), capturados por pescadores amadores em 5 diferentes pontos em locais próximos à Laguna dos Patos, Lagoa Mirim ou o Canal São Gonçalo. A prevalência de parasitismo por Clinostomum spp. com diagnóstico através de chaves parasitológicas foi de 36,21%, com variações da prevalência de acordo com os pontos relatados de captura dos espécimes de Jundiá, sendo confirmado a partir da PCR padronizada. Conclui-se que a prevalência é alta nos jundiás analisados e a PCR pode ser uma ferramenta auxiliar no diagnóstico desse parasita elevando não só a sensibilidade, mas também a rapidez do teste. Tais fatores serão de grande importância para futuros estudos epidemiológicos, assim como para saúde pública, visto que o consumo de peixes de água doce, típico da culinária oriental, encontra-se em expansão no Rio Grande do Sul e no Brasil.Research, Society and Development2021-09-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2092310.33448/rsd-v10i12.20923Research, Society and Development; Vol. 10 No. 12; e509101220923Research, Society and Development; Vol. 10 Núm. 12; e509101220923Research, Society and Development; v. 10 n. 12; e5091012209232525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/20923/18484Copyright (c) 2021 Iuri Vladimir Pioly Marmitt; João Pedro Mello Silva; Rodrigo Casquero Cunha; Samuel Rodrigues Felix; Flávia Aleixo Vasconcellos; Leandro Quintana Nizoli; Éverton Fagonde da Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMarmitt, Iuri Vladimir Pioly Silva, João Pedro Mello Cunha, Rodrigo Casquero Felix, Samuel Rodrigues Vasconcellos, Flávia Aleixo Nizoli, Leandro Quintana Silva, Éverton Fagonde da 2021-11-14T20:26:51Zoai:ojs.pkp.sfu.ca:article/20923Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:40:27.160915Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR Prevalencia de trematodos del género Clinostomum en Jundiás (Rhamdia quelen) y desarrollo de un método diagnóstico mediante PCR Prevalência de trematódeos do gênero Clinostomum em Jundiás (Rhamdia quelen) e desenvolvimento de um método de diagnóstico através de PCR |
title |
Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR |
spellingShingle |
Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR Marmitt, Iuri Vladimir Pioly Parasitism Public health Molecular diagnosis Fish. Parasitismo Saúde pública Diagnóstico molecular Peixes. Parasitismo Salud pública Diagnóstico molecular Peces. |
title_short |
Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR |
title_full |
Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR |
title_fullStr |
Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR |
title_full_unstemmed |
Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR |
title_sort |
Prevalence of trematodes of the genus Clinostomum in Jundiás (Rhamdia quelen) and development of a diagnostic method by PCR |
author |
Marmitt, Iuri Vladimir Pioly |
author_facet |
Marmitt, Iuri Vladimir Pioly Silva, João Pedro Mello Cunha, Rodrigo Casquero Felix, Samuel Rodrigues Vasconcellos, Flávia Aleixo Nizoli, Leandro Quintana Silva, Éverton Fagonde da |
author_role |
author |
author2 |
Silva, João Pedro Mello Cunha, Rodrigo Casquero Felix, Samuel Rodrigues Vasconcellos, Flávia Aleixo Nizoli, Leandro Quintana Silva, Éverton Fagonde da |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Marmitt, Iuri Vladimir Pioly Silva, João Pedro Mello Cunha, Rodrigo Casquero Felix, Samuel Rodrigues Vasconcellos, Flávia Aleixo Nizoli, Leandro Quintana Silva, Éverton Fagonde da |
dc.subject.por.fl_str_mv |
Parasitism Public health Molecular diagnosis Fish. Parasitismo Saúde pública Diagnóstico molecular Peixes. Parasitismo Salud pública Diagnóstico molecular Peces. |
topic |
Parasitism Public health Molecular diagnosis Fish. Parasitismo Saúde pública Diagnóstico molecular Peixes. Parasitismo Salud pública Diagnóstico molecular Peces. |
description |
Clinostomum complanatum Rudolphi, 1814 is a digenetic trematode that infects mollusks, several species of freshwater fish and piscivorous birds. In its life cycle, C. complanatum has as its second intermediate host some freshwater fish, among which Jundiá (Rhamdia quelen). Thus, the aim of the present study was to evaluate the prevalence of Clinostomum infections in Jundiás captured in the estuary region of Pelotas/RS and to standardize a Polymerase Chain Reaction (PCR) to diagnose the presence of metacercariae in tissue samples from fish captured in this estuary. In the cross-sectional study and the standardization of PCR, 174 samples of Jundiás (Rhamdia quelen), captured by amateur fishermen at 5 different points in locations close to Laguna dos Patos, Lagoa Mirim or the São Gonçalo Channel were used. The prevalence of parasitism by Clinostomum spp. with diagnosis through parasitological keys was 36.21%, with variations in prevalence according to the reported capture points of specimens from Jundiá, being confirmed by standardized PCR. It is concluded that the prevalence is high in the analyzed silver catfish and PCR can be an auxiliary tool in the diagnosis of this parasite, increasing not only the sensitivity, but also the speed of the test. Such factors will be of great importance for future epidemiological studies, as well as for public health, since the consumption of freshwater fish, typical of oriental cuisine, is expanding in Rio Grande do Sul and Brazil. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/20923 10.33448/rsd-v10i12.20923 |
url |
https://rsdjournal.org/index.php/rsd/article/view/20923 |
identifier_str_mv |
10.33448/rsd-v10i12.20923 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/20923/18484 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 12; e509101220923 Research, Society and Development; Vol. 10 Núm. 12; e509101220923 Research, Society and Development; v. 10 n. 12; e509101220923 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052641938767872 |