Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/13371 |
Resumo: | Objective: Evaluate the messenger RNAs (mRNA) PIWI proteins expression during infection with VDEN 4 in human hepatocyte cells. Materials and Methods: VDEN4 strain H778494 (JQ513335) was used, which was stored in Aedes albopictus cell culture (Clone C6 / 36) at the Arbovirology and Hemorrhagic Fevers section of the Evandro Chagas Institute. The techniques of cell cultures, stock (C6/36), inoculation, extraction, viral load quantification, RTqPCR and statistical analyzes were performed at the Viral Biogenesis Laboratory. Results: According to the results obtained, two cells (HepG2 and Huh7.5) demonstrated a higher level of viral replication at 72 hours post infection (hpi). The PIWI 2 target alter its mRNA expression during VDEN4 infection. The PIWI 4 target expression, was observed an altered expression in the infected cells. Thus, it was found that they are in different poles, while the viral load in the first three days showed high expression. The expression of mRNA was low in relation to the normal rate given by the uninfected cells. Conclusion: In our findings, it was observed that PIWI2 and 4 proteins have an inverse relationship to the viral infection process by VDEN4, when there is an increase in viral replication, these two proteins end up having a significant reduction in their expression. Probably, this reduction of expression is involved with the biogenesis process of apoptosis-regulating microRNAs. |
| id |
UNIFEI_41a89d4b652b04b5efed6c4b7d15e63a |
|---|---|
| oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/13371 |
| network_acronym_str |
UNIFEI |
| network_name_str |
Research, Society and Development |
| repository_id_str |
|
| spelling |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4Perfil de expresión de mRNAs de la família de proteína PIWI en células humanas infectadas experimentalmente por el Vírus Dengue 4Perfil da expressão dos mRNAs da família de proteínas PIWI em células humanas infectadas experimentalmente pelo Vírus Dengue 4 PIWImRNADengue VírusmicroRNA.PIWImRNADengue VírusmicroRNA.PIWImRNAVírus DenguemicroRNA.Objective: Evaluate the messenger RNAs (mRNA) PIWI proteins expression during infection with VDEN 4 in human hepatocyte cells. Materials and Methods: VDEN4 strain H778494 (JQ513335) was used, which was stored in Aedes albopictus cell culture (Clone C6 / 36) at the Arbovirology and Hemorrhagic Fevers section of the Evandro Chagas Institute. The techniques of cell cultures, stock (C6/36), inoculation, extraction, viral load quantification, RTqPCR and statistical analyzes were performed at the Viral Biogenesis Laboratory. Results: According to the results obtained, two cells (HepG2 and Huh7.5) demonstrated a higher level of viral replication at 72 hours post infection (hpi). The PIWI 2 target alter its mRNA expression during VDEN4 infection. The PIWI 4 target expression, was observed an altered expression in the infected cells. Thus, it was found that they are in different poles, while the viral load in the first three days showed high expression. The expression of mRNA was low in relation to the normal rate given by the uninfected cells. Conclusion: In our findings, it was observed that PIWI2 and 4 proteins have an inverse relationship to the viral infection process by VDEN4, when there is an increase in viral replication, these two proteins end up having a significant reduction in their expression. Probably, this reduction of expression is involved with the biogenesis process of apoptosis-regulating microRNAs.Objetivo: Evaluar la expresión de los ARN mensajeros (ARNm) de las proteínas PIWI durante la infección por VDEN 4 en células de hepatocitos humanos. Materiales y Métodos: Se utilizó la cepa VDEN4 H778494 (JQ513335), la cual se almacenó en cultivo celular de Aedes albopictus (Clon C6 / 36) en la colección de la sección de Arbovirología y Fiebres Hemorrágicas del Instituto Evandro Chagas. Las técnicas de cultivo celular, stock (C6/36), inoculación, extracción, cuantificación de carga viral, RTqPCR y análisis estadísticos se realizaron en el Laboratorio de Biogénesis Viral de la misma sección. Resultados: De acuerdo con los resultados obtenidos, se observó que dos células (HepG2 and Huh7.5), demostraron un mayor nivel de replicación viral a las 72 horas post infección (hpi). Se ha demostrado que la diana PIWI 2 altera su expresión de RNAm durante la infección por VDEN4. En la expresión de la diana PIWI 4, se observó una expresión alterada en las células infectadas. Así, se encontró que se encuentran en polos diferentes, mientras que el título viral en los primeros tres días mostró alta expresión, la expresión de RNAm fue baja en relación a la tasa normal dada por la muestra no infectada. Conclusión: en nuestros hallazgos se observó que las proteínas PIWI2 y 4 tienen una relación inversa con el proceso de infección viral por VDEN4, es decir, cuando hay un aumento en la replicación viral, estas proteínas terminan teniendo una reducción significativa en su expresión. . Estos datos terminan por llevarnos a considerar que esta reducción de expresión está involucrada en el proceso de biogénesis de los microRNA reguladores de la apoptosis.Objetivo: Avaliar a expressão dos RNAs mensageiros (mRNA) das proteínas PIWI durante a infecção por VDEN 4 em células de hepatócitos humano. Materiais e Métodos: Foi utilizada a cepa H778494 (JQ513335) do VDEN4 que se encontrava estocada em cultura de células Aedes albopictus (Clone C6/36) no acervo da seção de Arbovirologia e Febres Hemorrágicas do Instituto Evandro Chagas. As técnicas de cultivos celulares (C6/36, HepG2 e Huh7.5), estoque (C6/36), inoculação, extração, quantificação de carga viral, RTqPCR e análises estatísticas foram todas realizadas no Laboratório de Biogênese Viral da mesma seção. Resultados: De acordo com os resultados obtidos observou-se que duas células HepG2 e Huh7.5) demonstraram um maior nível de replicação viral a 72 horas pós infecção (hpi). O alvo PIWI 2 demonstrou que altera sua expressão de mRNA durante a infecção por VDEN4. Na expressão do alvo PIWI 4 foi observado uma expressão alterada nas células infectadas. Assim, verificou-se que os mesmos se encontram em polos diferentes, enquanto o titulo viral nos três primeiros dias apresentaram alta expressão, a expressão de mRNA foi baixa em relação a taxa normal dada pela amostra não infectada. Conclusão: Em nossos achados observou-se que as proteínas PIWI2 e 4 possuem uma relação inversa ao processo de infecção viral por VDEN4, isto é, quando ocorre um aumento da replicação viral essas duas proteínas acabam por ter uma redução significante de sua expressão. Estes dados acabam por nos levar a considerar que esta redução da expressão está envolvida com o processo de biogênese de microRNAs reguladores de apoptose.Research, Society and Development2021-03-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1337110.33448/rsd-v10i3.13371Research, Society and Development; Vol. 10 No. 3; e32010313371Research, Society and Development; Vol. 10 Núm. 3; e32010313371Research, Society and Development; v. 10 n. 3; e320103133712525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/13371/12030Copyright (c) 2021 Walter Félix Franco Neto; Murilo Tavares Amorim; Karla Fabiane Lopes de Melo; Gustavo Moraes Holanda; Jardel Fábio Lopes Ferreira; Samir Mansour Moraes Cassebhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFranco Neto, Walter Félix Amorim, Murilo TavaresMelo, Karla Fabiane Lopes de Holanda, Gustavo Moraes Ferreira, Jardel Fábio Lopes Casseb, Samir Mansour Moraes 2021-03-28T12:03:35Zoai:ojs.pkp.sfu.ca:article/13371Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:43.750602Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4 Perfil de expresión de mRNAs de la família de proteína PIWI en células humanas infectadas experimentalmente por el Vírus Dengue 4 Perfil da expressão dos mRNAs da família de proteínas PIWI em células humanas infectadas experimentalmente pelo Vírus Dengue 4 |
| title |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4 |
| spellingShingle |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4 Franco Neto, Walter Félix PIWI mRNA Dengue Vírus microRNA. PIWI mRNA Dengue Vírus microRNA. PIWI mRNA Vírus Dengue microRNA. |
| title_short |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4 |
| title_full |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4 |
| title_fullStr |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4 |
| title_full_unstemmed |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4 |
| title_sort |
Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4 |
| author |
Franco Neto, Walter Félix |
| author_facet |
Franco Neto, Walter Félix Amorim, Murilo Tavares Melo, Karla Fabiane Lopes de Holanda, Gustavo Moraes Ferreira, Jardel Fábio Lopes Casseb, Samir Mansour Moraes |
| author_role |
author |
| author2 |
Amorim, Murilo Tavares Melo, Karla Fabiane Lopes de Holanda, Gustavo Moraes Ferreira, Jardel Fábio Lopes Casseb, Samir Mansour Moraes |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Franco Neto, Walter Félix Amorim, Murilo Tavares Melo, Karla Fabiane Lopes de Holanda, Gustavo Moraes Ferreira, Jardel Fábio Lopes Casseb, Samir Mansour Moraes |
| dc.subject.por.fl_str_mv |
PIWI mRNA Dengue Vírus microRNA. PIWI mRNA Dengue Vírus microRNA. PIWI mRNA Vírus Dengue microRNA. |
| topic |
PIWI mRNA Dengue Vírus microRNA. PIWI mRNA Dengue Vírus microRNA. PIWI mRNA Vírus Dengue microRNA. |
| description |
Objective: Evaluate the messenger RNAs (mRNA) PIWI proteins expression during infection with VDEN 4 in human hepatocyte cells. Materials and Methods: VDEN4 strain H778494 (JQ513335) was used, which was stored in Aedes albopictus cell culture (Clone C6 / 36) at the Arbovirology and Hemorrhagic Fevers section of the Evandro Chagas Institute. The techniques of cell cultures, stock (C6/36), inoculation, extraction, viral load quantification, RTqPCR and statistical analyzes were performed at the Viral Biogenesis Laboratory. Results: According to the results obtained, two cells (HepG2 and Huh7.5) demonstrated a higher level of viral replication at 72 hours post infection (hpi). The PIWI 2 target alter its mRNA expression during VDEN4 infection. The PIWI 4 target expression, was observed an altered expression in the infected cells. Thus, it was found that they are in different poles, while the viral load in the first three days showed high expression. The expression of mRNA was low in relation to the normal rate given by the uninfected cells. Conclusion: In our findings, it was observed that PIWI2 and 4 proteins have an inverse relationship to the viral infection process by VDEN4, when there is an increase in viral replication, these two proteins end up having a significant reduction in their expression. Probably, this reduction of expression is involved with the biogenesis process of apoptosis-regulating microRNAs. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-03-17 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13371 10.33448/rsd-v10i3.13371 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/13371 |
| identifier_str_mv |
10.33448/rsd-v10i3.13371 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/13371/12030 |
| dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
| publisher.none.fl_str_mv |
Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 3; e32010313371 Research, Society and Development; Vol. 10 Núm. 3; e32010313371 Research, Society and Development; v. 10 n. 3; e32010313371 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
| instname_str |
Universidade Federal de Itajubá (UNIFEI) |
| instacron_str |
UNIFEI |
| institution |
UNIFEI |
| reponame_str |
Research, Society and Development |
| collection |
Research, Society and Development |
| repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
| repository.mail.fl_str_mv |
rsd.articles@gmail.com |
| _version_ |
1797052746501718016 |