Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium

Bibliographic Details
Main Author: Brandini, Daniela Atili
Publication Date: 2020
Other Authors: Beneti, Igor Mariotto, Debortoli, Caio Vinícius Lourenço, Amaral, Marina Fuzette, Côvre, Luiza Monzoli, Casatti, Cláudio Aparecido
Format: Article
Language: eng
Source: Research, Society and Development
Download full: https://rsdjournal.org/index.php/rsd/article/view/7719
Summary: Carbonic anhydrase II (CA II) is involved with the acid-base homeostasis of tissue. This study aims to evaluate the effect of traumatic dental occlusion (TDO) by means of CA II expression in osteoclasts and osteocytes (near the lamina dura and in the centre of alveolar bone septum), in the periodontal ligament (PDL) and in lining cells (periosteum). For this study, 50 male Wistar rats aged seven weeks were divided into 2 groups: Loaded and Unloaded group. The study periods were 2, 5, 7, 14 and 21 days. The Mann-Whitney U test for quantitative, and the Chi-square test for semi-quantitative analyses were used for group comparison, along with Bonferroni’s post-hoc test. Statistically significant differences between the groups were observed in the number of osteoclasts in the lamina dura (days 5, 7 and 21); the alveolar bone septum (days 2 and 7); osteocytes near the lamina dura (days 2, 5, 7 and 14); and in the centre of the alveolar bone septum (days 2, 5, 7 and 14). There were also differences between-group in CA II expression in the lining cells on days 7 and 14. TDO increases CA II expression in osteoclasts, osteocytes, the PDL and lining cells of the periosteum. Clinical Relevance: Traumatic dental occlusion stimulates higher cells activity of the alveolar bone at short (lamina dura) and long (centre of alveolar bone and periosteum) distances.
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spelling Mechanical loading on the functional activity of carbonic anhydrase II in the periodontiumCarga mecánica sobre la actividad funcional de la anhidrasa carbónica II en el periodontoCarga mecânica na atividade funcional da anidrase carbônica II no periodontoAnidrases CarbônicasPeriodontoOclusão dentária traumática.Anhidrasas CarbónicasPeriodoncioOclusión dental traumática.Carbonic AnhydrasesPeriodontiumTraumatic dental occlusion.Carbonic anhydrase II (CA II) is involved with the acid-base homeostasis of tissue. This study aims to evaluate the effect of traumatic dental occlusion (TDO) by means of CA II expression in osteoclasts and osteocytes (near the lamina dura and in the centre of alveolar bone septum), in the periodontal ligament (PDL) and in lining cells (periosteum). For this study, 50 male Wistar rats aged seven weeks were divided into 2 groups: Loaded and Unloaded group. The study periods were 2, 5, 7, 14 and 21 days. The Mann-Whitney U test for quantitative, and the Chi-square test for semi-quantitative analyses were used for group comparison, along with Bonferroni’s post-hoc test. Statistically significant differences between the groups were observed in the number of osteoclasts in the lamina dura (days 5, 7 and 21); the alveolar bone septum (days 2 and 7); osteocytes near the lamina dura (days 2, 5, 7 and 14); and in the centre of the alveolar bone septum (days 2, 5, 7 and 14). There were also differences between-group in CA II expression in the lining cells on days 7 and 14. TDO increases CA II expression in osteoclasts, osteocytes, the PDL and lining cells of the periosteum. Clinical Relevance: Traumatic dental occlusion stimulates higher cells activity of the alveolar bone at short (lamina dura) and long (centre of alveolar bone and periosteum) distances.La anhidrasa carbónica II (AC II) participa en la homeostasis ácido-base del tejido. Este estudio tiene como objetivo evaluar el efecto de la oclusión dental traumática (ODT) mediante la expresión de CA II en osteoclastos y osteocitos (cerca de la lámina dura y en el centro del tabique del hueso alveolar), en el ligamento periodontal (LP) y en las células de revestimiento. (periostio). Para este estudio, 50 ratas Wistar macho de siete semanas se dividieron en 2 grupos: un grupo cargado y un grupo descargado. Los periodos de estudio fueron 2, 5, 7, 14 y 21 días. La prueba U de Mann-Whitney para análisis cuantitativos y la prueba de Chi-cuadrado para análisis semicuantitativos se utilizaron para la comparación de grupos, junto con la prueba post-hoc de Bonferroni. Se observaron diferencias estadísticamente significativas entre los grupos en el número de osteoclastos en la lámina dura (días 5, 7 y 21); el tabique del hueso alveolar (días 2 y 7); osteocitos cerca de la lámina dura (días 2, 5, 7 y 14); y en el centro del tabique del hueso alveolar (días 2, 5, 7 y 14). También hubo diferencias entre los grupos en la expresión de AC II en las células de revestimiento los días 7 y 14. La ODT aumenta la expresión de AC II en los osteoclastos, osteocitos, el LP y las células de revestimiento del periostio. Relevancia clínica: la oclusión dental traumática estimula una mayor actividad de las células del hueso alveolar a distancias cortas (lámina dura) y largas (centro del hueso alveolar y periostio).A anidrase carbônica II (AC II) participa da homeostase ácido-básica do tecido. Este estudo tem como objetivo avaliar o efeito da oclusão dentária traumática (ODT) através da expressão de AC II em osteoclastos e osteócitos (próximo à lâmina dura e no centro do septo do osso alveolar), no ligamento periodontal (LP) e nas células de revestimento. (periósteo). Para este estudo, 50 ratos Wistar machos com sete semanas de idade foram divididos em 2 grupos: um grupo com carga e um grupo sem carga. Os períodos de estudo foram de 2, 5, 7, 14 e 21 dias. O teste U de Mann-Whitney para análise quantitativa e o teste Qui-quadrado para análise semiquantitativa foram usados ​​para a comparação dos grupos, juntamente com o teste post-hoc de Bonferroni. Diferenças estatisticamente significativas foram observadas entre os grupos no número de osteoclastos na lâmina dura (dias 5, 7 e 21); o septo do osso alveolar (dias 2 e 7); osteócitos próximos à lâmina dura (dias 2, 5, 7 e 14); e no centro do septo do osso alveolar (dias 2, 5, 7 e 14). Também houve diferenças de grupo na expressão de AC II em células de revestimento nos dias 7 e 14. ODT aumenta a expressão de AC II em osteoclastos, osteócitos, LP e células de revestimento periosteal. Relevância clínica: a oclusão dentária traumática estimula o aumento da atividade das células ósseas alveolares em distâncias curtas (lâmina dura) e longas (centro do osso alveolar e periósteo).Research, Society and Development2020-09-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/771910.33448/rsd-v9i9.7719Research, Society and Development; Vol. 9 No. 9; e686997719Research, Society and Development; Vol. 9 Núm. 9; e686997719Research, Society and Development; v. 9 n. 9; e6869977192525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/7719/6891Copyright (c) 2020 Daniela Atili Brandini; Igor Mariotto Beneti; Caio Vinícius Lourenço Debortoli; Marina Fuzette Amaral; Luiza Monzoli Côvre; Cláudio Aparecido Casattihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBrandini, Daniela Atili Beneti, Igor MariottoDebortoli, Caio Vinícius LourençoAmaral, Marina FuzetteCôvre, Luiza MonzoliCasatti, Cláudio Aparecido2020-09-18T01:42:11Zoai:ojs.pkp.sfu.ca:article/7719Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:30:21.584297Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium
Carga mecánica sobre la actividad funcional de la anhidrasa carbónica II en el periodonto
Carga mecânica na atividade funcional da anidrase carbônica II no periodonto
title Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium
spellingShingle Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium
Brandini, Daniela Atili
Anidrases Carbônicas
Periodonto
Oclusão dentária traumática.
Anhidrasas Carbónicas
Periodoncio
Oclusión dental traumática.
Carbonic Anhydrases
Periodontium
Traumatic dental occlusion.
title_short Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium
title_full Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium
title_fullStr Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium
title_full_unstemmed Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium
title_sort Mechanical loading on the functional activity of carbonic anhydrase II in the periodontium
author Brandini, Daniela Atili
author_facet Brandini, Daniela Atili
Beneti, Igor Mariotto
Debortoli, Caio Vinícius Lourenço
Amaral, Marina Fuzette
Côvre, Luiza Monzoli
Casatti, Cláudio Aparecido
author_role author
author2 Beneti, Igor Mariotto
Debortoli, Caio Vinícius Lourenço
Amaral, Marina Fuzette
Côvre, Luiza Monzoli
Casatti, Cláudio Aparecido
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brandini, Daniela Atili
Beneti, Igor Mariotto
Debortoli, Caio Vinícius Lourenço
Amaral, Marina Fuzette
Côvre, Luiza Monzoli
Casatti, Cláudio Aparecido
dc.subject.por.fl_str_mv Anidrases Carbônicas
Periodonto
Oclusão dentária traumática.
Anhidrasas Carbónicas
Periodoncio
Oclusión dental traumática.
Carbonic Anhydrases
Periodontium
Traumatic dental occlusion.
topic Anidrases Carbônicas
Periodonto
Oclusão dentária traumática.
Anhidrasas Carbónicas
Periodoncio
Oclusión dental traumática.
Carbonic Anhydrases
Periodontium
Traumatic dental occlusion.
description Carbonic anhydrase II (CA II) is involved with the acid-base homeostasis of tissue. This study aims to evaluate the effect of traumatic dental occlusion (TDO) by means of CA II expression in osteoclasts and osteocytes (near the lamina dura and in the centre of alveolar bone septum), in the periodontal ligament (PDL) and in lining cells (periosteum). For this study, 50 male Wistar rats aged seven weeks were divided into 2 groups: Loaded and Unloaded group. The study periods were 2, 5, 7, 14 and 21 days. The Mann-Whitney U test for quantitative, and the Chi-square test for semi-quantitative analyses were used for group comparison, along with Bonferroni’s post-hoc test. Statistically significant differences between the groups were observed in the number of osteoclasts in the lamina dura (days 5, 7 and 21); the alveolar bone septum (days 2 and 7); osteocytes near the lamina dura (days 2, 5, 7 and 14); and in the centre of the alveolar bone septum (days 2, 5, 7 and 14). There were also differences between-group in CA II expression in the lining cells on days 7 and 14. TDO increases CA II expression in osteoclasts, osteocytes, the PDL and lining cells of the periosteum. Clinical Relevance: Traumatic dental occlusion stimulates higher cells activity of the alveolar bone at short (lamina dura) and long (centre of alveolar bone and periosteum) distances.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/7719
10.33448/rsd-v9i9.7719
url https://rsdjournal.org/index.php/rsd/article/view/7719
identifier_str_mv 10.33448/rsd-v9i9.7719
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/7719/6891
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 9; e686997719
Research, Society and Development; Vol. 9 Núm. 9; e686997719
Research, Society and Development; v. 9 n. 9; e686997719
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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