Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora

Detalhes bibliográficos
Autor(a) principal: Diniz, Rosiane Aleixo
Data de Publicação: 2022
Outros Autores: Silva, Adrielle Antônia da, Cavalcante, Giani Maria, Lins, Luiz Andrade
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/28220
Resumo: This study aimed to evaluate the antitumor and inhibitory activity of human topoisomerase II-α of the flavonoid luteolin isolated from Prosopis juliflora against prostate tumor cells (PC-3), with the aim of investigating new drugs of natural origin. In vitro tests were conducted with crude leaf extracts against PC-3 and FGH cell lines. Cytotoxicity tests were performed using MTT (3-(4,5-dimethiazol-2yl) 2,5-diphenyl tetrazolium bromide) with monitoring after 24 h of exposure of tumor and non-tumor cells to different concentrations of extracts (100; 50; 25; 10 μg/mL). Luteolin extracted from the leaves of P. juliflora showed positive results on the DNA topo II-α enzyme essential in the replication process of cancer cells from the 50 µM concentration. Furthermore, it showed antitumor activity against PC-3 cells from the lowest concentration tested 10 µM, and absence of cytotoxicity against normal cells. A chemical study for the extraction and isolation of active compounds from P. juliflora leaf extracts is suggested, to investigate the possible antitumor activity of these compounds in in vitro and in vivo assays.
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spelling Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis julifloraActividad inhibidora de la topoisomerasa II-α antitumoral y humana del flavonoide luteolina aislado de Prosopis julifloraAtividade antitumoral e inibitória de topoisomerase II-α humana do flavonoide luteolina isolado de Prosopis julifloraAtividade antitumoralCâncer prostáticoProdutos naturaisProsopis juliflora.Antitumor activityProstate cancerNatural productsProsopis juliflora.Actividad antitumoralCancer de próstataProductos naturalesProsopis juliflora.This study aimed to evaluate the antitumor and inhibitory activity of human topoisomerase II-α of the flavonoid luteolin isolated from Prosopis juliflora against prostate tumor cells (PC-3), with the aim of investigating new drugs of natural origin. In vitro tests were conducted with crude leaf extracts against PC-3 and FGH cell lines. Cytotoxicity tests were performed using MTT (3-(4,5-dimethiazol-2yl) 2,5-diphenyl tetrazolium bromide) with monitoring after 24 h of exposure of tumor and non-tumor cells to different concentrations of extracts (100; 50; 25; 10 μg/mL). Luteolin extracted from the leaves of P. juliflora showed positive results on the DNA topo II-α enzyme essential in the replication process of cancer cells from the 50 µM concentration. Furthermore, it showed antitumor activity against PC-3 cells from the lowest concentration tested 10 µM, and absence of cytotoxicity against normal cells. A chemical study for the extraction and isolation of active compounds from P. juliflora leaf extracts is suggested, to investigate the possible antitumor activity of these compounds in in vitro and in vivo assays.Este estudio tuvo como objetivo evaluar la actividad antitumoral e inhibitoria de la topoisomerasa humana II-α del flavonoide luteolina aislado de Prosopis juliflora contra células tumorales de próstata (PC-3), con el objetivo de investigar nuevos fármacos de origen natural. Se realizaron pruebas in vitro con extractos de hojas crudas contra líneas celulares PC-3 y FGH. Se realizaron pruebas de citotoxicidad utilizando MTT (bromuro de 3-(4,5-dimetiazol-2il) 2,5-difeniltetrazolio) con seguimiento a las 24 h de exposición de células tumorales y no tumorales a diferentes concentraciones de extractos (100; 50; 25; 10 μg/mL). La luteolina extraída de las hojas de P. juliflora mostró resultados positivos en la enzima ADN topo II-α esencial en el proceso de replicación de las células cancerosas a partir de la concentración de 50 µM. Además, mostró actividad antitumoral frente a células PC-3 desde la concentración más baja ensayada 10 µM, y ausencia de citotoxicidad frente a células normales. Se sugiere un estudio químico para la extracción y aislamiento de compuestos activos de extractos de hojas de P. juliflora, para investigar la posible actividad antitumoral de estos compuestos en ensayos in vitro e in vivo.Este estudo objetivou avaliar a atividade antitumoral e inibitória da topoisomerase II-α humana do flavonoide luteolina isolado de Prosopis juliflora frente a células tumorais prostáticas (PC-3), com o intuito de investigação de novos fármacos de origem natural. Testes in vitro foram conduzidos com os extratos brutos das folhas frente as linhagens de células PC-3 e linhagem FGH. Os testes de citotoxicidade foram realizados através de MTT (3- (4,5-dimetiazol-2il) 2,5-difenil tetrazólio de brometo) com monitorização após 24 h de exposição das células tumorais e não tumorais a diferentes concentrações dos extratos (100; 50; 25; 10 μg/mL). A luteolina extraída das folhas da P. juliflora apresentou resultados positivos sobre a inibição de DNA topo II-α  enzima essencial no processo de replicação das células cancerígenas a partir da concetração 50 µM. Ademais, apresentou atividade antitumoral frente a células PC-3 a partir da menor concentração testada 10 µM, e ausência de citotoxicidade frente a células normais. Sugere-se o estudo químico para extração e isolamento de compostos ativos oriundo dos extratos de folha da P. juliflora, para investigar a possível atividade antitumoral destes compostos em ensaio in vitro e in vivo.Research, Society and Development2022-04-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2822010.33448/rsd-v11i5.28220Research, Society and Development; Vol. 11 No. 5; e47811528220Research, Society and Development; Vol. 11 Núm. 5; e47811528220Research, Society and Development; v. 11 n. 5; e478115282202525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/28220/24762Copyright (c) 2022 Rosiane Aleixo Diniz; Adrielle Antônia da Silva; Giani Maria Cavalcante; Luiz Andrade Linshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDiniz, Rosiane Aleixo Silva, Adrielle Antônia da Cavalcante, Giani MariaLins, Luiz Andrade2022-04-17T18:18:56Zoai:ojs.pkp.sfu.ca:article/28220Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:42.204422Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
Actividad inhibidora de la topoisomerasa II-α antitumoral y humana del flavonoide luteolina aislado de Prosopis juliflora
Atividade antitumoral e inibitória de topoisomerase II-α humana do flavonoide luteolina isolado de Prosopis juliflora
title Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
spellingShingle Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
Diniz, Rosiane Aleixo
Atividade antitumoral
Câncer prostático
Produtos naturais
Prosopis juliflora.
Antitumor activity
Prostate cancer
Natural products
Prosopis juliflora.
Actividad antitumoral
Cancer de próstata
Productos naturales
Prosopis juliflora.
title_short Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
title_full Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
title_fullStr Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
title_full_unstemmed Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
title_sort Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
author Diniz, Rosiane Aleixo
author_facet Diniz, Rosiane Aleixo
Silva, Adrielle Antônia da
Cavalcante, Giani Maria
Lins, Luiz Andrade
author_role author
author2 Silva, Adrielle Antônia da
Cavalcante, Giani Maria
Lins, Luiz Andrade
author2_role author
author
author
dc.contributor.author.fl_str_mv Diniz, Rosiane Aleixo
Silva, Adrielle Antônia da
Cavalcante, Giani Maria
Lins, Luiz Andrade
dc.subject.por.fl_str_mv Atividade antitumoral
Câncer prostático
Produtos naturais
Prosopis juliflora.
Antitumor activity
Prostate cancer
Natural products
Prosopis juliflora.
Actividad antitumoral
Cancer de próstata
Productos naturales
Prosopis juliflora.
topic Atividade antitumoral
Câncer prostático
Produtos naturais
Prosopis juliflora.
Antitumor activity
Prostate cancer
Natural products
Prosopis juliflora.
Actividad antitumoral
Cancer de próstata
Productos naturales
Prosopis juliflora.
description This study aimed to evaluate the antitumor and inhibitory activity of human topoisomerase II-α of the flavonoid luteolin isolated from Prosopis juliflora against prostate tumor cells (PC-3), with the aim of investigating new drugs of natural origin. In vitro tests were conducted with crude leaf extracts against PC-3 and FGH cell lines. Cytotoxicity tests were performed using MTT (3-(4,5-dimethiazol-2yl) 2,5-diphenyl tetrazolium bromide) with monitoring after 24 h of exposure of tumor and non-tumor cells to different concentrations of extracts (100; 50; 25; 10 μg/mL). Luteolin extracted from the leaves of P. juliflora showed positive results on the DNA topo II-α enzyme essential in the replication process of cancer cells from the 50 µM concentration. Furthermore, it showed antitumor activity against PC-3 cells from the lowest concentration tested 10 µM, and absence of cytotoxicity against normal cells. A chemical study for the extraction and isolation of active compounds from P. juliflora leaf extracts is suggested, to investigate the possible antitumor activity of these compounds in in vitro and in vivo assays.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/28220
10.33448/rsd-v11i5.28220
url https://rsdjournal.org/index.php/rsd/article/view/28220
identifier_str_mv 10.33448/rsd-v11i5.28220
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/28220/24762
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 5; e47811528220
Research, Society and Development; Vol. 11 Núm. 5; e47811528220
Research, Society and Development; v. 11 n. 5; e47811528220
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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