Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies

Detalhes bibliográficos
Autor(a) principal: Cruz, Luisa Ferreira da
Data de Publicação: 2021
Outros Autores: Santos, Cicero Gonzaga, Gonçalves, Thais Paula Rodrigues, Marena, Gabriel Davi, Souza, Israel Lucas Antunes, Lima, Luciana Alves Rodrigues dos Santos, Chequer, Farah Maria Drumond, Pinto, Flavia Carmo Horta, Nogueira, Flavia Aparecida Resende, Araujo, Marcelo Gonzaga de Freitas
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/16948
Resumo: 4-hydroxycoumarin (4H) is a natural and synthetic phenolic compound that presents important attributes, including anti-HIV, antitumor, and anticoagulant properties. Considering its important biological activities and the use of 4H, the aim of this present study was to investigate the toxic, mutagenic, antimutagenic, and antioxidant activities of 4H, commercially obtained, in experiments in vivo and in vitro. The effects of acute oral administration at a dose of 200 mg/kg of 4H were investigated by the hippocratic screening in animals. The micronucleus assay on peripheral blood and the Ames test were used for analysis of mutagenicity, while the antimutagenicity was tested against cyclophosphamide also by in vivo micronucleus assay. For all in vivo tests were used Swiss mice. The antioxidant activity was determined by the capacity of the 4H sample to sequester the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. The results showed that 4H no induced changes in the behavior or the histology of treated animals neither mutagenic activity (genic or chromosomal). On the other hand, it exhibited a protective effect against cyclophosphamide and antioxidant potential. 4- hydroxycoumarin is widely used clinically as a precursor molecule in the synthesis of synthetic anticoagulant drugs. Therefore, the results obtained are extremely important, as they demonstrate the absence of mutagenic potential and protection against chromosomal damage.
id UNIFEI_46a28287ca80a5c6ddabd97341cf03b8
oai_identifier_str oai:ojs.pkp.sfu.ca:article/16948
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studiesEvaluación toxicológica de la 4-hidroxicumarina: Estudios (anti) mutagénicos, tóxicos y antioxidantesAvaliação toxicológica da 4-hidroxicumarina: (Anti) Mutagenicidade, estudos de toxicidade e antioxidantesAntioxidanteCumarinasGenotoxicidadPruebas de Mutagenicidad.AntioxidanteCumarinasGenotoxicidadeTeste de MutagenicidadeTeste para Micronúcleo.AntioxidantsCoumarinsGenotoxicityMicronucleus testsMutagenicity Tests.4-hydroxycoumarin (4H) is a natural and synthetic phenolic compound that presents important attributes, including anti-HIV, antitumor, and anticoagulant properties. Considering its important biological activities and the use of 4H, the aim of this present study was to investigate the toxic, mutagenic, antimutagenic, and antioxidant activities of 4H, commercially obtained, in experiments in vivo and in vitro. The effects of acute oral administration at a dose of 200 mg/kg of 4H were investigated by the hippocratic screening in animals. The micronucleus assay on peripheral blood and the Ames test were used for analysis of mutagenicity, while the antimutagenicity was tested against cyclophosphamide also by in vivo micronucleus assay. For all in vivo tests were used Swiss mice. The antioxidant activity was determined by the capacity of the 4H sample to sequester the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. The results showed that 4H no induced changes in the behavior or the histology of treated animals neither mutagenic activity (genic or chromosomal). On the other hand, it exhibited a protective effect against cyclophosphamide and antioxidant potential. 4- hydroxycoumarin is widely used clinically as a precursor molecule in the synthesis of synthetic anticoagulant drugs. Therefore, the results obtained are extremely important, as they demonstrate the absence of mutagenic potential and protection against chromosomal damage.La 4-hidroxicumarina (4H) es un compuesto fenólico natural y sintético que presenta importantes atributos, que incluyen propiedades anti-VIH, antitumorales y anticoagulantes. Considerando sus importantes actividades biológicas y el uso de 4H, el objetivo de este estudio fue investigar las actividades tóxicas, mutagénicas, antimutagénicas y antioxidantes de 4H, obtenidas comercialmente, en experimentos in vivo e in vitro. Los efectos de la administración oral aguda a una dosis de 200 mg / kg de 4H se investigaron mediante el cribado hipocrático en animales. El ensayo de micronúcleos en sangre periférica y la prueba de Ames se utilizaron para el análisis de mutagenicidad, mientras que la antimutagenicidad se probó contra ciclofosfamida también mediante ensayo de micronúcleos in vivo. Para todas las pruebas in vivo se utilizaron ratones suizos. La actividad antioxidante se determinó por la capacidad de la muestra de 4H para secuestrar el radical estable 2,2-difenil-1-picrilhidrazilo (DPPH). Los resultados mostraron que 4H no indujo cambios en el comportamiento o la histología de los animales tratados ni actividad mutagénica (génica o cromosómica). Por otro lado, exhibió un efecto protector contra la ciclofosfamida y potencial antioxidante. La 4H se utiliza ampliamente en la clínica como molécula precursora en la síntesis de fármacos anticoagulantes sintéticos. Por tanto, los resultados obtenidos son de suma importancia, ya que demuestran la ausencia de potencial mutagénico y protección frente al daño cromosómico.A 4-hidroxicumarina (4H) é um composto fenólico natural e sintético que apresenta atributos importantes, incluindo propriedades anti-HIV, antitumoral e anticoagulante. Considerando suas importantes atividades biológicas e a utilização da 4H, o objetivo do presente estudo foi investigar as atividades tóxica, mutagênica, antimutagênica e antioxidante da 4H, obtido comercialmente, em experimentos in vivo e in vitro. Os efeitos da administração oral aguda na dose de 200 mg / kg de 4H foram investigados pela triagem hipocrática em animais. O ensaio de micronúcleo em sangue periférico e o teste de Ames foram utilizados para análise de mutagenicidade, enquanto a antimutagenicidade foi testada contra a ciclofosfamida também pelo ensaio de micronúcleo in vivo. Para todos os testes in vivo foram utilizados camundongos Swiss. A atividade antioxidante foi determinada pela capacidade da amostra 4H de sequestrar o radical estável 2,2-difenil-1-picrilhidrazil (DPPH). Os resultados mostraram que o 4H não induziu alterações no comportamento ou na histologia dos animais tratados nem na atividade mutagênica (gênica ou cromossômica). Por outro lado, exibiu efeito protetor contra ciclofosfamida e potencial antioxidante. A 4H é amplamente utilizada clinicamente como uma molécula precursora na síntese de fármacos anticoagulantes sintéticos. Portanto, os resultados obtidos são de extrema importância, pois demonstram ausência de potencial mutagênico e proteção contra danos cromossômicos.Research, Society and Development2021-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1694810.33448/rsd-v10i8.16948Research, Society and Development; Vol. 10 No. 8; e7910816948Research, Society and Development; Vol. 10 Núm. 8; e7910816948Research, Society and Development; v. 10 n. 8; e79108169482525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/16948/15265Copyright (c) 2021 Luisa Ferreira da Cruz; Cicero Gonzaga Santos; Thais Paula Rodrigues Gonçalves; Gabriel Davi Marena; Israel Lucas Antunes Souza; Luciana Alves Rodrigues dos Santos Lima; Farah Maria Drumond Chequer; Flavia Carmo Horta Pinto; Flavia Aparecida Resende Nogueira; Marcelo Gonzaga de Freitas Araujohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCruz, Luisa Ferreira daSantos, Cicero GonzagaGonçalves, Thais Paula Rodrigues Marena, Gabriel DaviSouza, Israel Lucas AntunesLima, Luciana Alves Rodrigues dos SantosChequer, Farah Maria Drumond Pinto, Flavia Carmo Horta Nogueira, Flavia Aparecida ResendeAraujo, Marcelo Gonzaga de Freitas 2021-08-21T18:46:59Zoai:ojs.pkp.sfu.ca:article/16948Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:22.138509Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies
Evaluación toxicológica de la 4-hidroxicumarina: Estudios (anti) mutagénicos, tóxicos y antioxidantes
Avaliação toxicológica da 4-hidroxicumarina: (Anti) Mutagenicidade, estudos de toxicidade e antioxidantes
title Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies
spellingShingle Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies
Cruz, Luisa Ferreira da
Antioxidante
Cumarinas
Genotoxicidad
Pruebas de Mutagenicidad.
Antioxidante
Cumarinas
Genotoxicidade
Teste de Mutagenicidade
Teste para Micronúcleo.
Antioxidants
Coumarins
Genotoxicity
Micronucleus tests
Mutagenicity Tests.
title_short Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies
title_full Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies
title_fullStr Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies
title_full_unstemmed Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies
title_sort Toxicological assessment of 4-hydroxycoumarin: (Anti) Mutagenicity, toxic, and antioxidant studies
author Cruz, Luisa Ferreira da
author_facet Cruz, Luisa Ferreira da
Santos, Cicero Gonzaga
Gonçalves, Thais Paula Rodrigues
Marena, Gabriel Davi
Souza, Israel Lucas Antunes
Lima, Luciana Alves Rodrigues dos Santos
Chequer, Farah Maria Drumond
Pinto, Flavia Carmo Horta
Nogueira, Flavia Aparecida Resende
Araujo, Marcelo Gonzaga de Freitas
author_role author
author2 Santos, Cicero Gonzaga
Gonçalves, Thais Paula Rodrigues
Marena, Gabriel Davi
Souza, Israel Lucas Antunes
Lima, Luciana Alves Rodrigues dos Santos
Chequer, Farah Maria Drumond
Pinto, Flavia Carmo Horta
Nogueira, Flavia Aparecida Resende
Araujo, Marcelo Gonzaga de Freitas
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cruz, Luisa Ferreira da
Santos, Cicero Gonzaga
Gonçalves, Thais Paula Rodrigues
Marena, Gabriel Davi
Souza, Israel Lucas Antunes
Lima, Luciana Alves Rodrigues dos Santos
Chequer, Farah Maria Drumond
Pinto, Flavia Carmo Horta
Nogueira, Flavia Aparecida Resende
Araujo, Marcelo Gonzaga de Freitas
dc.subject.por.fl_str_mv Antioxidante
Cumarinas
Genotoxicidad
Pruebas de Mutagenicidad.
Antioxidante
Cumarinas
Genotoxicidade
Teste de Mutagenicidade
Teste para Micronúcleo.
Antioxidants
Coumarins
Genotoxicity
Micronucleus tests
Mutagenicity Tests.
topic Antioxidante
Cumarinas
Genotoxicidad
Pruebas de Mutagenicidad.
Antioxidante
Cumarinas
Genotoxicidade
Teste de Mutagenicidade
Teste para Micronúcleo.
Antioxidants
Coumarins
Genotoxicity
Micronucleus tests
Mutagenicity Tests.
description 4-hydroxycoumarin (4H) is a natural and synthetic phenolic compound that presents important attributes, including anti-HIV, antitumor, and anticoagulant properties. Considering its important biological activities and the use of 4H, the aim of this present study was to investigate the toxic, mutagenic, antimutagenic, and antioxidant activities of 4H, commercially obtained, in experiments in vivo and in vitro. The effects of acute oral administration at a dose of 200 mg/kg of 4H were investigated by the hippocratic screening in animals. The micronucleus assay on peripheral blood and the Ames test were used for analysis of mutagenicity, while the antimutagenicity was tested against cyclophosphamide also by in vivo micronucleus assay. For all in vivo tests were used Swiss mice. The antioxidant activity was determined by the capacity of the 4H sample to sequester the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. The results showed that 4H no induced changes in the behavior or the histology of treated animals neither mutagenic activity (genic or chromosomal). On the other hand, it exhibited a protective effect against cyclophosphamide and antioxidant potential. 4- hydroxycoumarin is widely used clinically as a precursor molecule in the synthesis of synthetic anticoagulant drugs. Therefore, the results obtained are extremely important, as they demonstrate the absence of mutagenic potential and protection against chromosomal damage.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16948
10.33448/rsd-v10i8.16948
url https://rsdjournal.org/index.php/rsd/article/view/16948
identifier_str_mv 10.33448/rsd-v10i8.16948
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16948/15265
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 8; e7910816948
Research, Society and Development; Vol. 10 Núm. 8; e7910816948
Research, Society and Development; v. 10 n. 8; e7910816948
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052786790105088