Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis

Detalhes bibliográficos
Autor(a) principal: Curi, Marco Antonio Maluf
Data de Publicação: 2021
Outros Autores: Borges, Mariana Castro Loureiro, Geraldo-Martins, Vinicius Rangel, Silva, Camila Beatriz, Rodrigues, Rafael Rocha, Rodrigues, Denise Bertulucci Rocha, Pereira, Sanivia Aparecida Lima, Nogueira, Ruchele Dias
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/23791
Resumo: This study investigated and compared the presence of Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans (Aa) in biological samples of women submitted to the term delivery diagnosed with gingivitis and healthy periodontium. Oral clinical exams were performed in 48 puerperal women including 28 women with healthy periodontium, 15 with gingivitis and 5 with periodontitis (that were excluded of analysis). Samples of umbilical cord blood (CB), peripheral blood (PB), saliva (SA) and colostrum (C) were collected from eligible women after delivery. DNA extraction was accomplished, and the presence of bacteria was detected by PCR with primers and specific probes for each microorganism. Data about previous and current pregnancies and known risk factors were obtained from patients’ medical records. Social, demographic, and oral care data was analyzed by interview. All salivas of participants with gingivitis presented Pg and Aa, while a minority of samples of with healthy periodontium had those bacteria (P<0.05 for each). Sixty percent of the women with gingivitis presented Pg in the blood samples (PB and CB, p<0.05) and 46.6% presented in C. Aa did not present in the blood samples, but 46.6% of women with gingivitis had Aa in the C. No association between socioeconomic and oral health data, detection of microorganisms and presence of the disease. Gingivitis was related to the positive detection of both microorganisms in saliva. Women with gingivitis presented Pg in PB and CB. The presence of Pg and Aa in colostrum occurred only in pregnant women with gingivitis.
id UNIFEI_488e3c0e43ce8a2d43cb7f362216fc58
oai_identifier_str oai:ojs.pkp.sfu.ca:article/23791
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitisPorphyromonas gingivalis y Aggregatibacter actinomycetemcomitans en sangre de cordón umbilical y periférico, saliva y calostro de mujeres con gingivitisPorphyromonas gingivalis e Aggregatibacter actinomycetemcomitans em sangue do cordão umbilical e periférico, saliva e colostro de mulheres com gengivitePorphyromonas gingivalisAggregatibacter actinomycetemcomitans Sangue do cordão umbilicalSalivaColostro.Porphyromonas gingivalisAggregatibacter actinomycetemcomitansCordón umbilical sangreSalivaColostrum.Porphyromonas gingivalisAggregatibacter actinomycetemcomitans Umbilical cord bloodSalivaColostrum.This study investigated and compared the presence of Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans (Aa) in biological samples of women submitted to the term delivery diagnosed with gingivitis and healthy periodontium. Oral clinical exams were performed in 48 puerperal women including 28 women with healthy periodontium, 15 with gingivitis and 5 with periodontitis (that were excluded of analysis). Samples of umbilical cord blood (CB), peripheral blood (PB), saliva (SA) and colostrum (C) were collected from eligible women after delivery. DNA extraction was accomplished, and the presence of bacteria was detected by PCR with primers and specific probes for each microorganism. Data about previous and current pregnancies and known risk factors were obtained from patients’ medical records. Social, demographic, and oral care data was analyzed by interview. All salivas of participants with gingivitis presented Pg and Aa, while a minority of samples of with healthy periodontium had those bacteria (P<0.05 for each). Sixty percent of the women with gingivitis presented Pg in the blood samples (PB and CB, p<0.05) and 46.6% presented in C. Aa did not present in the blood samples, but 46.6% of women with gingivitis had Aa in the C. No association between socioeconomic and oral health data, detection of microorganisms and presence of the disease. Gingivitis was related to the positive detection of both microorganisms in saliva. Women with gingivitis presented Pg in PB and CB. The presence of Pg and Aa in colostrum occurred only in pregnant women with gingivitis.Este estudio investigó y comparó la presencia de Porphyromonas gingivalis (Pg) y Aggregatibacter actinomycetemcomitans (Aa) en muestras biológicas de mujeres que dieron a luz a término con un diagnóstico de gingivitis y sin enfermedad periodontal. Se realizaron exámenes clínicos orales en 48 mujeres posparto, incluidas 28 mujeres con periodontitis sana, 15 con gingivitis y 5 con periodontitis (que fueron excluidas del análisis). Se recolectaron sangre de cordón umbilical (CU), sangre periférica (SP), saliva (SA) y calostro (C) de mujeres elegibles después del parto. Se realizó extracción de ADN y luego se detectó la presencia de bacterias mediante PCR con cebadores específicos. Los datos sobre embarazos anteriores y actuales y factores de riesgo conocidos se obtuvieron de los registros médicos de las pacientes. Los datos sociales, demográficos y de higiene bucal se analizaron mediante entrevista. Los resultados mostraron que todos los EA de los participantes con gingivitis tenían Pg y Aa, mientras que una minoría de muestras con periodonto sano tenían tales bacterias (P <0,05 para cada una). El 60% de las mujeres con gingivitis tenían Pg en las muestras de sangre (CU y SP, p <0,05) y el 46,6% la tenían en C. Aa no aparecía en las muestras de sangre, pero el 46,6% de las mujeres con gingivitis tenían Aa en C. sin asociación entre datos socioeconómicos y de salud bucal, detección de microorganismos y presencia de la enfermedad. En conclusión, la gingivitis se relacionó con la detección positiva de ambas bacterias en SA. Las mujeres con gingivitis a menudo tenían Pg en sus muestras de sangre. La presencia de Pg y Aa en C solo se presentó en mujeres embarazadas con gingivitis.Este estudo investigou e comparou a presença de Porphyromonas gingivalis (Pg) e Aggregatibacter actinomycetemcomitans (Aa) em amostras biológicas de mulheres que tiveram parto a termo com diagnóstico de gengivite e sem doença periodontal. Exames clínicos orais foram realizados em 48 puérperas, incluindo 28 mulheres com periodonto saudável, 15 com gengivite e 5 com periodontite (que foram excluídas da análise). Sangue do cordão umbilical (CU), sangue periférico (SP), saliva (SA) e colostro (C) foram coletados de mulheres elegíveis após o parto. Foi realizada extração de DNA e em seguida a presença de bactérias foi detectada por PCR com primers específicos. Dados sobre gestações anteriores e atuais e fatores de risco conhecidos foram obtidos dos prontuários médicos dos pacientes. Os dados sociais, demográficos e de higiene bucal foram analisados por entrevista. Os resultados mostraram que todas as SA das participantes com gengivite apresentou Pg e Aa, enquanto a minoria das amostras com periodonto saudável apresentou tais bactérias (P <0,05 para cada uma). 60% das mulheres com gengivite apresentaram Pg nas amostras de sangue (CU e SP, p <0,05) e 46,6% apresentaram no C. Aa não apareceu nas amostras de sangue, mas 46,6% das mulheres com gengivite apresentaram Aa em C. Não houve associação entre dados socioeconômicos e de saúde bucal, detecção de microrganismos e presença da doença. Em conclusão, a gengivite esteve relacionada com a detecção positiva de ambas as bactérias em SA. Mulheres com gengivite apresentaram frequentemente Pg nas amostras sanguíneas. A presença de Pg e Aa em C ocorreu apenas em gestantes com gengivite.Research, Society and Development2021-12-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2379110.33448/rsd-v10i16.23791Research, Society and Development; Vol. 10 No. 16; e372101623791Research, Society and Development; Vol. 10 Núm. 16; e372101623791Research, Society and Development; v. 10 n. 16; e3721016237912525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/23791/21019Copyright (c) 2021 Marco Antonio Maluf Curi; Mariana Castro Loureiro Borges; Vinicius Rangel Geraldo-Martins; Camila Beatriz Silva; Rafael Rocha Rodrigues; Denise Bertulucci Rocha Rodrigues; Sanivia Aparecida Lima Pereira; Ruchele Dias Nogueirahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCuri, Marco Antonio MalufBorges, Mariana Castro LoureiroGeraldo-Martins, Vinicius RangelSilva, Camila BeatrizRodrigues, Rafael RochaRodrigues, Denise Bertulucci RochaPereira, Sanivia Aparecida LimaNogueira, Ruchele Dias2021-12-20T11:03:07Zoai:ojs.pkp.sfu.ca:article/23791Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:32.870691Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis
Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans en sangre de cordón umbilical y periférico, saliva y calostro de mujeres con gingivitis
Porphyromonas gingivalis e Aggregatibacter actinomycetemcomitans em sangue do cordão umbilical e periférico, saliva e colostro de mulheres com gengivite
title Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis
spellingShingle Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis
Curi, Marco Antonio Maluf
Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Sangue do cordão umbilical
Saliva
Colostro.
Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Cordón umbilical sangre
Saliva
Colostrum.
Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Umbilical cord blood
Saliva
Colostrum.
title_short Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis
title_full Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis
title_fullStr Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis
title_full_unstemmed Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis
title_sort Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in umbilical cord and peripheral blood, saliva, colostrum from women with gingivitis
author Curi, Marco Antonio Maluf
author_facet Curi, Marco Antonio Maluf
Borges, Mariana Castro Loureiro
Geraldo-Martins, Vinicius Rangel
Silva, Camila Beatriz
Rodrigues, Rafael Rocha
Rodrigues, Denise Bertulucci Rocha
Pereira, Sanivia Aparecida Lima
Nogueira, Ruchele Dias
author_role author
author2 Borges, Mariana Castro Loureiro
Geraldo-Martins, Vinicius Rangel
Silva, Camila Beatriz
Rodrigues, Rafael Rocha
Rodrigues, Denise Bertulucci Rocha
Pereira, Sanivia Aparecida Lima
Nogueira, Ruchele Dias
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Curi, Marco Antonio Maluf
Borges, Mariana Castro Loureiro
Geraldo-Martins, Vinicius Rangel
Silva, Camila Beatriz
Rodrigues, Rafael Rocha
Rodrigues, Denise Bertulucci Rocha
Pereira, Sanivia Aparecida Lima
Nogueira, Ruchele Dias
dc.subject.por.fl_str_mv Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Sangue do cordão umbilical
Saliva
Colostro.
Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Cordón umbilical sangre
Saliva
Colostrum.
Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Umbilical cord blood
Saliva
Colostrum.
topic Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Sangue do cordão umbilical
Saliva
Colostro.
Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Cordón umbilical sangre
Saliva
Colostrum.
Porphyromonas gingivalis
Aggregatibacter actinomycetemcomitans
Umbilical cord blood
Saliva
Colostrum.
description This study investigated and compared the presence of Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans (Aa) in biological samples of women submitted to the term delivery diagnosed with gingivitis and healthy periodontium. Oral clinical exams were performed in 48 puerperal women including 28 women with healthy periodontium, 15 with gingivitis and 5 with periodontitis (that were excluded of analysis). Samples of umbilical cord blood (CB), peripheral blood (PB), saliva (SA) and colostrum (C) were collected from eligible women after delivery. DNA extraction was accomplished, and the presence of bacteria was detected by PCR with primers and specific probes for each microorganism. Data about previous and current pregnancies and known risk factors were obtained from patients’ medical records. Social, demographic, and oral care data was analyzed by interview. All salivas of participants with gingivitis presented Pg and Aa, while a minority of samples of with healthy periodontium had those bacteria (P<0.05 for each). Sixty percent of the women with gingivitis presented Pg in the blood samples (PB and CB, p<0.05) and 46.6% presented in C. Aa did not present in the blood samples, but 46.6% of women with gingivitis had Aa in the C. No association between socioeconomic and oral health data, detection of microorganisms and presence of the disease. Gingivitis was related to the positive detection of both microorganisms in saliva. Women with gingivitis presented Pg in PB and CB. The presence of Pg and Aa in colostrum occurred only in pregnant women with gingivitis.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/23791
10.33448/rsd-v10i16.23791
url https://rsdjournal.org/index.php/rsd/article/view/23791
identifier_str_mv 10.33448/rsd-v10i16.23791
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/23791/21019
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 16; e372101623791
Research, Society and Development; Vol. 10 Núm. 16; e372101623791
Research, Society and Development; v. 10 n. 16; e372101623791
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052698321747968

Registros relacionados