Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers

Detalhes bibliográficos
Autor(a) principal: Menezes Filho, Antonio Carlos Pereira de
Data de Publicação: 2020
Outros Autores: Romão, Luciene Teixeira Gonçalves, Reis, Eloisa Borges dos, Malaquias, Karla da Silva, Castro, Carlos Frederico de Souza, Marcionilio, Suzana Maria Loures de Oliveira, Oliveira, Marilene Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/5979
Resumo: The study of chromatographic techniques, classical and modern, describes the simplicity and, at the same time, the advances that this area has undergone in recent years in quality scientific research and also in learning in undergraduate and postgraduate courses around the world. This paper investigate a characterization by thin layer chromatography (TLC) and gas chromatography coupled with mass spectrometry (GC-MS), as a method developed by graduate students that involve a combination of a classic and modern technique, as well as results about the physicochemical properties of the essential oil of Bauhinia monandra flower. Essential oil was extracted by Clevenger, the TLC was performed in different eluents and developers, and thus the retention factors (Rfs), and the chemical profile by GC-MS were obtained. The essential oil of the flowers showed a yield of 0.06%, positive solubility in ethanol 70%, refractive index of 1.3621, optical rotation of +36.4αD and relative density of 0.941 g mL-1 at 20 °C. In the TLC analysis 18 Rfs were observed after the use of different developers, with the predominant class of oxygenates compounds. In the GC-MS analysis, 7 compounds were observed, being two majorities, characterized as panaxene with 20.51% and the α-guaiene with 33.39%. The essential oil of B. monandra flower showed a predominance of 70.22% of sesquiterpenic compounds. The allied techniques, classic and modern, demonstrated different ways of evaluating the essential oil through its chemical composition, both techniques showed high efficiency and precision, in addition was an appropriate project developed by postgraduate students.
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spelling Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowersAnálisis cromatográficas y evaluación fisicoquímico del aceite esencial de las flores de Bauhinia monandra KurzAnálise cromatográfica e avaliação físico-química do óleo essencial das flores de Bauhinia monandra KurzCromatografiaÓleo essencialGênero Bauhinia.CromatografíaAceite esencialGénero Bauhinia.ChromatographyEssential oilGenus Bauhinia.The study of chromatographic techniques, classical and modern, describes the simplicity and, at the same time, the advances that this area has undergone in recent years in quality scientific research and also in learning in undergraduate and postgraduate courses around the world. This paper investigate a characterization by thin layer chromatography (TLC) and gas chromatography coupled with mass spectrometry (GC-MS), as a method developed by graduate students that involve a combination of a classic and modern technique, as well as results about the physicochemical properties of the essential oil of Bauhinia monandra flower. Essential oil was extracted by Clevenger, the TLC was performed in different eluents and developers, and thus the retention factors (Rfs), and the chemical profile by GC-MS were obtained. The essential oil of the flowers showed a yield of 0.06%, positive solubility in ethanol 70%, refractive index of 1.3621, optical rotation of +36.4αD and relative density of 0.941 g mL-1 at 20 °C. In the TLC analysis 18 Rfs were observed after the use of different developers, with the predominant class of oxygenates compounds. In the GC-MS analysis, 7 compounds were observed, being two majorities, characterized as panaxene with 20.51% and the α-guaiene with 33.39%. The essential oil of B. monandra flower showed a predominance of 70.22% of sesquiterpenic compounds. The allied techniques, classic and modern, demonstrated different ways of evaluating the essential oil through its chemical composition, both techniques showed high efficiency and precision, in addition was an appropriate project developed by postgraduate students.El estudio de las técnicas cromatográficas, clásicas y modernas, describe la simplicidad y, al mismo tiempo, los avances que esta área ha sufrido en los últimos años en la investigación científica de calidad y también en el aprendizaje de cursos de pregrado y posgrado en todo el mundo. En este trabajo, se presenta una caracterización por cromatografía de capa fina (CCF) y cromatografía gaseosa con espectrometría de masas (CG-EM) como método desarrollado por estudiantes de posgrado, que implica una combinación de técnicas clásicas y modernas, así como propiedades físicas y químicas del aceite esencial de flores de Bauhinia monandra. El aceite esencial fue extraído por Clevenger, el CCF se realizó en diferentes eluyentes y desarrolladores, obteniendo los factores de retención Rfs y el perfil químico por CG-EM. El aceite esencial de las flores mostró un rendimiento de 0,06%, solubilidad positiva en etanol al 70%, índice de refracción de 1,3621, rotación óptica de +36,4 αD y densidad relativa de 0,941 g mL-1 a 20 °C y en CCF 18 Rfs se observaron después de usar diferentes desarrolladores, con la clase predominante de oxigenados. En el análisis CG-MS, se observaron 7 compuestos, dos de los cuales fueron principales, caracterizados como panaxeno con 20,51% y α-guaieno con 33,39%. El aceite esencial de la flor de B. monandra mostró un predominio del 70,22% de los compuestos sesquiterpénicos. Las técnicas aliadas, clásicas y modernas, demostraron diferentes formas de evaluar el aceite esencial a través de su composición química, ambas con alta eficiencia y precisión, además de ser un proyecto apropiado para ser desarrollado por estudiantes graduados.O estudo das técnicas cromatográficas, clássicas e modernas, descreve a simplicidade e, ao mesmo tempo, os avanços que essa área vem sofrendo nos últimos anos em pesquisas científicas de qualidade e também na aprendizagem de cursos de graduação e pós-graduação em todo o mundo. Este trabalho apresenta uma caracterização por cromatografia em camada delgada (CCD) e cromatografia gasosa acoplada à espectrometria de massa (CG-EM), como método desenvolvido por estudantes de pós-graduação que envolve a combinação de uma técnica clássica e moderna bem como propriedades físico químicas do óleo essencial das flores de Bauhinia monandra. O óleo essencial foi extraído por Clevenger, a CCD foi realizada em diferentes eluentes e reveladores, deste modo obteve-se os fatores de retenção Rfs e o perfil químico por CG-EM. O óleo essencial das flores apresentou rendimento de 0,06%, uma solubilidade positiva em etanol 70%, índice de refração de 1,3621, rotação óptica de +36,4 αD e densidade relativa de 0,941 g mL-1 a 20 °C e na CCD 18 Rfs foram observados após o uso de diferentes reveladores, com a classe predominante de oxigenados. Na análise por CG-EM, foram observados 7 compostos, sendo dois majoritários, caracterizados como panaxeno com 20,51% e α-guaieno com 33,39%. O óleo essencial da flor de B. monandra apresentou predominância de 70,22% dos compostos sesquiterpênicos. As técnicas aliadas, clássicas e modernas, demonstraram diferentes formas de avaliar o óleo essencial por meio de sua composição química, ambas com alta eficiência e precisão, além de ser um projeto apropriado para ser desenvolvido por estudantes de pós-graduação.Research, Society and Development2020-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/597910.33448/rsd-v9i8.5979Research, Society and Development; Vol. 9 No. 8; e454985979Research, Society and Development; Vol. 9 Núm. 8; e454985979Research, Society and Development; v. 9 n. 8; e4549859792525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/5979/5850Copyright (c) 2020 Marilene Silva Oliveirahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMenezes Filho, Antonio Carlos Pereira deRomão, Luciene Teixeira GonçalvesReis, Eloisa Borges dosMalaquias, Karla da SilvaCastro, Carlos Frederico de SouzaMarcionilio, Suzana Maria Loures de OliveiraOliveira, Marilene Silva2020-08-20T18:00:17Zoai:ojs.pkp.sfu.ca:article/5979Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:29:17.890833Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers
Análisis cromatográficas y evaluación fisicoquímico del aceite esencial de las flores de Bauhinia monandra Kurz
Análise cromatográfica e avaliação físico-química do óleo essencial das flores de Bauhinia monandra Kurz
title Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers
spellingShingle Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers
Menezes Filho, Antonio Carlos Pereira de
Cromatografia
Óleo essencial
Gênero Bauhinia.
Cromatografía
Aceite esencial
Género Bauhinia.
Chromatography
Essential oil
Genus Bauhinia.
title_short Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers
title_full Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers
title_fullStr Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers
title_full_unstemmed Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers
title_sort Chromatographic analysis and physicochemical evaluation of the essential oil of Bauhinia monandra Kurz flowers
author Menezes Filho, Antonio Carlos Pereira de
author_facet Menezes Filho, Antonio Carlos Pereira de
Romão, Luciene Teixeira Gonçalves
Reis, Eloisa Borges dos
Malaquias, Karla da Silva
Castro, Carlos Frederico de Souza
Marcionilio, Suzana Maria Loures de Oliveira
Oliveira, Marilene Silva
author_role author
author2 Romão, Luciene Teixeira Gonçalves
Reis, Eloisa Borges dos
Malaquias, Karla da Silva
Castro, Carlos Frederico de Souza
Marcionilio, Suzana Maria Loures de Oliveira
Oliveira, Marilene Silva
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Menezes Filho, Antonio Carlos Pereira de
Romão, Luciene Teixeira Gonçalves
Reis, Eloisa Borges dos
Malaquias, Karla da Silva
Castro, Carlos Frederico de Souza
Marcionilio, Suzana Maria Loures de Oliveira
Oliveira, Marilene Silva
dc.subject.por.fl_str_mv Cromatografia
Óleo essencial
Gênero Bauhinia.
Cromatografía
Aceite esencial
Género Bauhinia.
Chromatography
Essential oil
Genus Bauhinia.
topic Cromatografia
Óleo essencial
Gênero Bauhinia.
Cromatografía
Aceite esencial
Género Bauhinia.
Chromatography
Essential oil
Genus Bauhinia.
description The study of chromatographic techniques, classical and modern, describes the simplicity and, at the same time, the advances that this area has undergone in recent years in quality scientific research and also in learning in undergraduate and postgraduate courses around the world. This paper investigate a characterization by thin layer chromatography (TLC) and gas chromatography coupled with mass spectrometry (GC-MS), as a method developed by graduate students that involve a combination of a classic and modern technique, as well as results about the physicochemical properties of the essential oil of Bauhinia monandra flower. Essential oil was extracted by Clevenger, the TLC was performed in different eluents and developers, and thus the retention factors (Rfs), and the chemical profile by GC-MS were obtained. The essential oil of the flowers showed a yield of 0.06%, positive solubility in ethanol 70%, refractive index of 1.3621, optical rotation of +36.4αD and relative density of 0.941 g mL-1 at 20 °C. In the TLC analysis 18 Rfs were observed after the use of different developers, with the predominant class of oxygenates compounds. In the GC-MS analysis, 7 compounds were observed, being two majorities, characterized as panaxene with 20.51% and the α-guaiene with 33.39%. The essential oil of B. monandra flower showed a predominance of 70.22% of sesquiterpenic compounds. The allied techniques, classic and modern, demonstrated different ways of evaluating the essential oil through its chemical composition, both techniques showed high efficiency and precision, in addition was an appropriate project developed by postgraduate students.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/5979
10.33448/rsd-v9i8.5979
url https://rsdjournal.org/index.php/rsd/article/view/5979
identifier_str_mv 10.33448/rsd-v9i8.5979
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/5979/5850
dc.rights.driver.fl_str_mv Copyright (c) 2020 Marilene Silva Oliveira
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Marilene Silva Oliveira
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 8; e454985979
Research, Society and Development; Vol. 9 Núm. 8; e454985979
Research, Society and Development; v. 9 n. 8; e454985979
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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