Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/36652 |
Resumo: | Fibrinolytic enzymes are proteases that are capable of degrading the fibrin mesh present in blood clots and can be obtained through fungal extracts. Thus, the objective of this paper was to seek out and compare all the information regarding the production and biochemical characterization of fibrinolytic enzymes of mushroom species published in papers in the last ten years. The research was carried out in eight national and international electronic databases using the terms: “fungal enzymes”, “fibrinolytic Proteases”, “thrombosis” and “mushrooms”. The results obtained were analyzed according to each step. In the first stage, the titles and abstracts of all papers were analyzed independently; in the second stage, all papers were read and those that did not meet the inclusion criteria were excluded. Then, the remaining papers were reviewed and the data were tabulated and compared. As a result, it was found that enzymes with fibrinolytic action were obtained from eight species of mushrooms in the period of this research: P. sajor-cashew, P. ferulae, P. ostreatus, L. shimeji, A. polytricha, H. erinaceum, C. comatus and C. militaris. The enzymes were identified as protease SPPs, metalloproteases, serine proteases and serine metalloproteases. The methods used to evaluate fibrinolytic activity were the fibrin plate method and/or the formation of artificial thrombus through thrombin and fibrinogen. The molecular mass of these enzymes ranged from ~18 to 66 kDa and, for the biochemical characterization of the extracts, the pH ranged from 4.0 to 9.5 and the optimum temperature from 25 to 70 ºC. Via the review, it was observed that few articles were published on the subject in question, which made it necessary to carry out more studies to discover the biotechnological properties of mushrooms and define their applications in the most diverse sectors of industries, not only in commercial species of mushrooms, but also in native species. |
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Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years Condiciones de cultivo y caracterización bioquímica de las enzimas proteolíticas con acción fibrinolítica obtenidas a partir de hongos de los últimos diez añosCondições de cultivo e caracterização bioquímica das enzimas proteolíticas com ação fibrinolítica obtidas a partir de cogumelos nos últimos dez anos Proteases FibrinolíticaCogumelosTerapia TrombolíticaFibrina.Proteasas fibrinolíticasHongosTerapia trombolíticaFibrina.Fibrinolytic ProteasesMushroomThrombolytic TherapyFibrin.Fibrinolytic enzymes are proteases that are capable of degrading the fibrin mesh present in blood clots and can be obtained through fungal extracts. Thus, the objective of this paper was to seek out and compare all the information regarding the production and biochemical characterization of fibrinolytic enzymes of mushroom species published in papers in the last ten years. The research was carried out in eight national and international electronic databases using the terms: “fungal enzymes”, “fibrinolytic Proteases”, “thrombosis” and “mushrooms”. The results obtained were analyzed according to each step. In the first stage, the titles and abstracts of all papers were analyzed independently; in the second stage, all papers were read and those that did not meet the inclusion criteria were excluded. Then, the remaining papers were reviewed and the data were tabulated and compared. As a result, it was found that enzymes with fibrinolytic action were obtained from eight species of mushrooms in the period of this research: P. sajor-cashew, P. ferulae, P. ostreatus, L. shimeji, A. polytricha, H. erinaceum, C. comatus and C. militaris. The enzymes were identified as protease SPPs, metalloproteases, serine proteases and serine metalloproteases. The methods used to evaluate fibrinolytic activity were the fibrin plate method and/or the formation of artificial thrombus through thrombin and fibrinogen. The molecular mass of these enzymes ranged from ~18 to 66 kDa and, for the biochemical characterization of the extracts, the pH ranged from 4.0 to 9.5 and the optimum temperature from 25 to 70 ºC. Via the review, it was observed that few articles were published on the subject in question, which made it necessary to carry out more studies to discover the biotechnological properties of mushrooms and define their applications in the most diverse sectors of industries, not only in commercial species of mushrooms, but also in native species.Las enzimas fibrinolíticas son proteasas que degradan la fibrina presente en los coágulos sanguíneos y pueden obtenerse a través de extractos fúngicos. Por tanto, el objetivo de este trabajo fue analizar toda la información sobre la producción y caracterización bioquímica de proteasas fibrinolíticas de especies de hongos publicada en artículos de los últimos diez años. La investigación se realizó en ocho bases de datos electrónicas nacionales e internacionales utilizando varios términos clave. Los resultados obtenidos se analizaron de acuerdo a cada paso. En la primera etapa, los títulos y resúmenes de todos los trabajos fueron analizados de forma independiente; en la segunda etapa se leyeron todos los trabajos y se excluyeron aquellos que no cumplían con los criterios de inclusión. Se revisaron los documentos restantes y se tabularon y compararon los datos. Como resultado se encontró que se obtuvieron enzimas con acción fibrinolítica de ocho especies de hongos en el periodo de esta investigación: P. sajor-cashew, P. ferulae, P. ostreatus, L. shimeji, A. polytricha, H. erinaceum, C. comatus y C. militaris. Las enzimas se identificaron como proteasas SPPs, metaloproteasas, serina proteasas y serina metaloproteasas. Además, Se han identificado dos métodos para determinar la actividad fibrinolítica, el método de placas de fibrina y/o la formación de trombos artificiales. La masa molecular de estas enzimas osciló entre ~18 y 66 kDa y, para la caracterización bioquímica de los extractos, el pH osciló entre 4,0 y 9,5 y la temperatura óptima entre 25 y 70 ºC. A través de la revisión se constató que los hongos son fuente de proteasas fibrinoliticas e que estos pueden actuar para el tratamiento de la trombosis.Enzimas fibrinolíticas são proteases que degradam a fibrina presente em coágulos sanguíneos e podem ser obtidas através de extratos fúngicos. Assim, o objetivo do estudo foi analisar todas as informações referentes a produção e caracterização de proteases fibrinolíticas de espécies de cogumelos publicados em plataformas cientificas dos últimos dez anos. A busca foi realizada em oito bases de dados eletrônicos nacionais e internacionais utilizando diversos palavras-chave. Os resultados obtidos foram analisados de acordo com cada etapa: na primeira etapa, os títulos e resumos de todos os artigos foram analisados de forma independente; na segunda etapa todos os artigos foram lidos e aqueles que não atendiam aos critérios de inclusão, foram excluídos. Em seguida foram revisados os artigos restantes e os dados tabulados e comparados. Foi verificado que enzimas com ação fibrinolíticas foram obtidas de oito espécies de cogumelos: P. sajor-caju, P. ferulae, P. ostreatus, L. shimeji, A. polytricha, H. erinaceum, C. comatus e C. militaris. As enzimas foram identificadas como protease SPPS, Metaloprotease, Serina Aspártica e Serina Metaloprotease. Além disso, dois métodos de determinação da atividade fibrinolítica foram identificados, o método da placa de fibrina e por formação do trombo artificial. A massa molecular das enzimas variou entre ~18 a 66 kDa, a caracterização bioquímica dos extratos teve o pH de 4,0 a 9,5 com temperatura ótima de 25 a 70 ºC. Dessa forma, foi possível concluir que os cogumelos são fontes de proteases fibrinolíticas e que podem ser indicados no tratamento de trombose.Research, Society and Development2022-11-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3665210.33448/rsd-v11i14.36652Research, Society and Development; Vol. 11 No. 14; e530111436652Research, Society and Development; Vol. 11 Núm. 14; e530111436652Research, Society and Development; v. 11 n. 14; e5301114366522525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/36652/30614Copyright (c) 2022 Romário da Silva Santana; Viviane Gonçalves de Farias; Elison de Souza Sevalho; Kadmiel Candido; Klaramelia Consuelo Ramón Carpio; Waldireny Rocha Gomes; Rosany Piccolotto Carvalhohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantana, Romário da SilvaFarias, Viviane Gonçalves deSevalho, Elison de Souza Candido, KadmielCarpio, Klaramelia Consuelo Ramón Gomes, Waldireny Rocha Carvalho, Rosany Piccolotto 2022-11-08T13:36:27Zoai:ojs.pkp.sfu.ca:article/36652Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:51:07.798601Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years Condiciones de cultivo y caracterización bioquímica de las enzimas proteolíticas con acción fibrinolítica obtenidas a partir de hongos de los últimos diez años Condições de cultivo e caracterização bioquímica das enzimas proteolíticas com ação fibrinolítica obtidas a partir de cogumelos nos últimos dez anos |
title |
Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years |
spellingShingle |
Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years Santana, Romário da Silva Proteases Fibrinolítica Cogumelos Terapia Trombolítica Fibrina. Proteasas fibrinolíticas Hongos Terapia trombolítica Fibrina. Fibrinolytic Proteases Mushroom Thrombolytic Therapy Fibrin. |
title_short |
Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years |
title_full |
Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years |
title_fullStr |
Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years |
title_full_unstemmed |
Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years |
title_sort |
Cultivation conditions and biochemical characterization of the proteolytic enzymes with fibrinolytic action obtained from mushrooms in the last ten years |
author |
Santana, Romário da Silva |
author_facet |
Santana, Romário da Silva Farias, Viviane Gonçalves de Sevalho, Elison de Souza Candido, Kadmiel Carpio, Klaramelia Consuelo Ramón Gomes, Waldireny Rocha Carvalho, Rosany Piccolotto |
author_role |
author |
author2 |
Farias, Viviane Gonçalves de Sevalho, Elison de Souza Candido, Kadmiel Carpio, Klaramelia Consuelo Ramón Gomes, Waldireny Rocha Carvalho, Rosany Piccolotto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Santana, Romário da Silva Farias, Viviane Gonçalves de Sevalho, Elison de Souza Candido, Kadmiel Carpio, Klaramelia Consuelo Ramón Gomes, Waldireny Rocha Carvalho, Rosany Piccolotto |
dc.subject.por.fl_str_mv |
Proteases Fibrinolítica Cogumelos Terapia Trombolítica Fibrina. Proteasas fibrinolíticas Hongos Terapia trombolítica Fibrina. Fibrinolytic Proteases Mushroom Thrombolytic Therapy Fibrin. |
topic |
Proteases Fibrinolítica Cogumelos Terapia Trombolítica Fibrina. Proteasas fibrinolíticas Hongos Terapia trombolítica Fibrina. Fibrinolytic Proteases Mushroom Thrombolytic Therapy Fibrin. |
description |
Fibrinolytic enzymes are proteases that are capable of degrading the fibrin mesh present in blood clots and can be obtained through fungal extracts. Thus, the objective of this paper was to seek out and compare all the information regarding the production and biochemical characterization of fibrinolytic enzymes of mushroom species published in papers in the last ten years. The research was carried out in eight national and international electronic databases using the terms: “fungal enzymes”, “fibrinolytic Proteases”, “thrombosis” and “mushrooms”. The results obtained were analyzed according to each step. In the first stage, the titles and abstracts of all papers were analyzed independently; in the second stage, all papers were read and those that did not meet the inclusion criteria were excluded. Then, the remaining papers were reviewed and the data were tabulated and compared. As a result, it was found that enzymes with fibrinolytic action were obtained from eight species of mushrooms in the period of this research: P. sajor-cashew, P. ferulae, P. ostreatus, L. shimeji, A. polytricha, H. erinaceum, C. comatus and C. militaris. The enzymes were identified as protease SPPs, metalloproteases, serine proteases and serine metalloproteases. The methods used to evaluate fibrinolytic activity were the fibrin plate method and/or the formation of artificial thrombus through thrombin and fibrinogen. The molecular mass of these enzymes ranged from ~18 to 66 kDa and, for the biochemical characterization of the extracts, the pH ranged from 4.0 to 9.5 and the optimum temperature from 25 to 70 ºC. Via the review, it was observed that few articles were published on the subject in question, which made it necessary to carry out more studies to discover the biotechnological properties of mushrooms and define their applications in the most diverse sectors of industries, not only in commercial species of mushrooms, but also in native species. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/36652 10.33448/rsd-v11i14.36652 |
url |
https://rsdjournal.org/index.php/rsd/article/view/36652 |
identifier_str_mv |
10.33448/rsd-v11i14.36652 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/36652/30614 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 14; e530111436652 Research, Society and Development; Vol. 11 Núm. 14; e530111436652 Research, Society and Development; v. 11 n. 14; e530111436652 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052727390371840 |