Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas

Detalhes bibliográficos
Autor(a) principal: Deboni, Tarita Cira
Data de Publicação: 2021
Outros Autores: Cargnelutti, Denise, Ghizzoni, Júlia Carolina, Santiago, Gabriela de Melo, Garbin, Egabrieli, Loregian, Alexandre Copatti, Giesel, Alexandre, Boff, Pedro, Petry, Cláudia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/18457
Resumo: The objective of this work was to evaluate peroxidase activity and protein concentration in the defence system expression of common bean Phaseolus vulgaris L., submitted to different potencies of homeopathic preparations. The treatments were composed by: Arsenicum album, Staphisagria, Silicea and Sulphur, in the 6, 12 and 18 CH potencies, compared to the controls. The bean seeds were sown in pots which were kept in a greenhouse and the treatments were applied to them at 1% in distilled water since the sowing day, with intervals of 15 days until the R7 stage. The first evaluation was performed at 15 e 60 days after sowing, after artificial mechanical damage. Protein concentration and the activity of guaiacol peroxidase (POD) and ascorbate peroxidase (APX) enzymes were determined. Differences between treatments and controls were estimated by Glass Delta effect size analysis. Staphisagria 12CH induced greater increase in protein concentration on bean leaves. The Induction on APX and POD activities were observed in leaves treated with Sulphur in all tested potencies. The Glass Delta effect showed undulatory behaviour, sometimes acting positively, sometimes negatively, throughout all evaluations. Homeopathic preparations have the potential to induce biochemical defence mechanisms in beans, acting as resistance elicitors.
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spelling Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticasActividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticasAtividade de peroxidase e concentração de proteína em Phaseolus vulgaris l. tratado com preparações homeopáticasEnzimasAltas diluições dinamizadasHomeopatiaAgroecologia.EnzimasAltas diluciones optimizadasHomeopatíaAgroecología.EnzymesHigh dynamized dilutionsHomeopathyAgroecology.The objective of this work was to evaluate peroxidase activity and protein concentration in the defence system expression of common bean Phaseolus vulgaris L., submitted to different potencies of homeopathic preparations. The treatments were composed by: Arsenicum album, Staphisagria, Silicea and Sulphur, in the 6, 12 and 18 CH potencies, compared to the controls. The bean seeds were sown in pots which were kept in a greenhouse and the treatments were applied to them at 1% in distilled water since the sowing day, with intervals of 15 days until the R7 stage. The first evaluation was performed at 15 e 60 days after sowing, after artificial mechanical damage. Protein concentration and the activity of guaiacol peroxidase (POD) and ascorbate peroxidase (APX) enzymes were determined. Differences between treatments and controls were estimated by Glass Delta effect size analysis. Staphisagria 12CH induced greater increase in protein concentration on bean leaves. The Induction on APX and POD activities were observed in leaves treated with Sulphur in all tested potencies. The Glass Delta effect showed undulatory behaviour, sometimes acting positively, sometimes negatively, throughout all evaluations. Homeopathic preparations have the potential to induce biochemical defence mechanisms in beans, acting as resistance elicitors.El objetivo de este trabajo fue evaluar la actividad peroxidasa y la concentración de proteínas en la expresión del sistema de defensa de Phaseolus vulgaris L., sometido a diferentes preparaciones homeopáticas. Los tratamientos consistieron en: Arsenicum album, Staphisagria, Silicea y Sulphur, en las potencias 6, 12 y 18 CH, en comparación con los controles. Las semillas se sembraron en macetas mantenidas en invernadero. Los tratamientos se aplicaron al 1% en agua destilada de la siembra, con intervalos de 15 días hasta la etapa R7. La primera evaluación se realizó a los 15 y 60 días después de la siembra, luego de un daño mecánico artificial. Se determinó la concentración de proteínas, la actividad de las enzimas guayacol peroxidasa (POD) y ascorbato peroxidasa (APX). Las diferencias entre tratamientos y controles se estimaron analizando el tamaño del efecto Glass Delta. Staphisagria 12CH indujo un mayor aumento en la concentración de proteínas en las hojas de frijol. Se observaron actividades de inducción de APX y POD en hojas tratadas con azufre en todas las potencias probadas. El efecto Glass Delta mostró un comportamiento oscilatorio en todas las evaluaciones. Las preparaciones homeopáticas tienen el potencial de inducir mecanismos de defensa bioquímicos en Phaseolus vulgaris L., actuando como inductores de resistencia.O objetivo deste trabalho foi avaliar a atividade da peroxidase e a concentração de proteínas na expressão do sistema de defesa de Phaseolus vulgaris L., submetido a diferentes preparações homeopáticas. Os tratamentos foram compostos por: Arsenicum album, Staphisagria, Silicea e Sulphur, nas potências 6, 12 e 18 CH, comparados aos controles. As sementes foram semeadas em vasos mantidos em casa de vegetação. Os tratamentos foram aplicados a 1% em água destilada a partir da semeadura, com intervalos de 15 dias até o estádio R7. A primeira avaliação foi realizada 15 e 60 dias após a semeadura, após danos mecânicos artificiais. A concentração de proteínas, atividade das enzimas guaiacol peroxidase (POD) e ascorbato peroxidase (APX) foram determinadas. As diferenças entre os tratamentos e os controles foram estimadas pela análise do tamanho do efeito Glass Delta. Staphisagria 12CH induziu maior aumento na concentração de proteína nas folhas do feijoeiro. As atividades de indução de APX e POD foram observadas em folhas tratadas com Sulphur em todas as potências testadas. O efeito Glass Delta apresentou comportamento oscilatório, em todas as avaliações. As preparações homeopáticas têm potencial para induzir mecanismos de defesa bioquímica em Phaseolus vulgaris L., atuando como eliciadores de resistência.Research, Society and Development2021-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1845710.33448/rsd-v10i9.18457Research, Society and Development; Vol. 10 No. 9; e59110918457Research, Society and Development; Vol. 10 Núm. 9; e59110918457Research, Society and Development; v. 10 n. 9; e591109184572525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/18457/16477Copyright (c) 2021 Tarita Cira Deboni; Denise Cargnelutti; Júlia Carolina Ghizzoni; Gabriela de Melo Santiago; Egabrieli Garbin; Alexandre Copatti Loregian; Alexandre Giesel; Pedro Boff; Cláudia Petryhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDeboni, Tarita Cira Cargnelutti, DeniseGhizzoni, Júlia Carolina Santiago, Gabriela de Melo Garbin, Egabrieli Loregian, Alexandre Copatti Giesel, Alexandre Boff, PedroPetry, Cláudia2021-09-12T14:28:06Zoai:ojs.pkp.sfu.ca:article/18457Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:38:35.580412Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
Atividade de peroxidase e concentração de proteína em Phaseolus vulgaris l. tratado com preparações homeopáticas
title Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
spellingShingle Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
Deboni, Tarita Cira
Enzimas
Altas diluições dinamizadas
Homeopatia
Agroecologia.
Enzimas
Altas diluciones optimizadas
Homeopatía
Agroecología.
Enzymes
High dynamized dilutions
Homeopathy
Agroecology.
title_short Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
title_full Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
title_fullStr Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
title_full_unstemmed Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
title_sort Actividad peroxidasa y concentración de proteínas en Phaseolus vulgaris l. tratado con preparaciones homeopáticas
author Deboni, Tarita Cira
author_facet Deboni, Tarita Cira
Cargnelutti, Denise
Ghizzoni, Júlia Carolina
Santiago, Gabriela de Melo
Garbin, Egabrieli
Loregian, Alexandre Copatti
Giesel, Alexandre
Boff, Pedro
Petry, Cláudia
author_role author
author2 Cargnelutti, Denise
Ghizzoni, Júlia Carolina
Santiago, Gabriela de Melo
Garbin, Egabrieli
Loregian, Alexandre Copatti
Giesel, Alexandre
Boff, Pedro
Petry, Cláudia
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Deboni, Tarita Cira
Cargnelutti, Denise
Ghizzoni, Júlia Carolina
Santiago, Gabriela de Melo
Garbin, Egabrieli
Loregian, Alexandre Copatti
Giesel, Alexandre
Boff, Pedro
Petry, Cláudia
dc.subject.por.fl_str_mv Enzimas
Altas diluições dinamizadas
Homeopatia
Agroecologia.
Enzimas
Altas diluciones optimizadas
Homeopatía
Agroecología.
Enzymes
High dynamized dilutions
Homeopathy
Agroecology.
topic Enzimas
Altas diluições dinamizadas
Homeopatia
Agroecologia.
Enzimas
Altas diluciones optimizadas
Homeopatía
Agroecología.
Enzymes
High dynamized dilutions
Homeopathy
Agroecology.
description The objective of this work was to evaluate peroxidase activity and protein concentration in the defence system expression of common bean Phaseolus vulgaris L., submitted to different potencies of homeopathic preparations. The treatments were composed by: Arsenicum album, Staphisagria, Silicea and Sulphur, in the 6, 12 and 18 CH potencies, compared to the controls. The bean seeds were sown in pots which were kept in a greenhouse and the treatments were applied to them at 1% in distilled water since the sowing day, with intervals of 15 days until the R7 stage. The first evaluation was performed at 15 e 60 days after sowing, after artificial mechanical damage. Protein concentration and the activity of guaiacol peroxidase (POD) and ascorbate peroxidase (APX) enzymes were determined. Differences between treatments and controls were estimated by Glass Delta effect size analysis. Staphisagria 12CH induced greater increase in protein concentration on bean leaves. The Induction on APX and POD activities were observed in leaves treated with Sulphur in all tested potencies. The Glass Delta effect showed undulatory behaviour, sometimes acting positively, sometimes negatively, throughout all evaluations. Homeopathic preparations have the potential to induce biochemical defence mechanisms in beans, acting as resistance elicitors.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18457
10.33448/rsd-v10i9.18457
url https://rsdjournal.org/index.php/rsd/article/view/18457
identifier_str_mv 10.33448/rsd-v10i9.18457
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/18457/16477
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 9; e59110918457
Research, Society and Development; Vol. 10 Núm. 9; e59110918457
Research, Society and Development; v. 10 n. 9; e59110918457
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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