Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.

Detalhes bibliográficos
Autor(a) principal: Batista, Anny Karoline Rodrigues
Data de Publicação: 2021
Outros Autores: Cesar, Kellyane Karen Ferreira Aguiar, Paula, Luciana Rocha, Silva, Francisco Laurindo da, Raposo, Haysha Laianne Oliveira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/14950
Resumo: Bacterial resistance to antibiotics has become a serious public health problem worldwide, affecting thousands of people. Thus, there is a need to seek alternative ways to combat them. Therefore, this research aimed to verify the antimicrobial effect of the extract of Annona muricata L. against ATCC strains of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. The material was collected and the extracts were obtained, then the bacterial strains were prepared for susceptibility testing. The tests proceeded in three stages: crude extracts, chemically fractionated extracts and tests with serial dilutions. Soursop antibacterial activity was observed against all strains tested, the most significant result being the crude extract of the fruit against the strain of E. coli with a halo equal to 22mm. In relation to the tests with the extracts already purified, the fractions from the fruit have more relevant action. The Hexane fraction corresponded to the most expressive results, obtaining halos equal to 28mm, 26mm and 37mm against the strains of E. coli, K. pneumoniae, and S. aureus respectively. As for the tests related to serial dilutions, we observed that after the 1/10 dilution, the extracts no longer had any inhibitory activity on the bacteria under study. However, these results demonstrate the potential of the soursop extract, especially the fruit, as a source of antibacterial compounds. However, it is worthwhile to continue more advanced research with Annona muricata.
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spelling Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.Potencial antibacteriano (in vitro) del extracto metanólico de Annona muricata L.Potencial antibacteriano (in vitro) do extrato metanólico da Annona muricata L. Extratos vegetaisBactériasTeste de suscetibilidade.Extractos de plantasBacteriasPrueba de susceptibilidad.Plant extractsBacteriaSusceptibility test.Bacterial resistance to antibiotics has become a serious public health problem worldwide, affecting thousands of people. Thus, there is a need to seek alternative ways to combat them. Therefore, this research aimed to verify the antimicrobial effect of the extract of Annona muricata L. against ATCC strains of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. The material was collected and the extracts were obtained, then the bacterial strains were prepared for susceptibility testing. The tests proceeded in three stages: crude extracts, chemically fractionated extracts and tests with serial dilutions. Soursop antibacterial activity was observed against all strains tested, the most significant result being the crude extract of the fruit against the strain of E. coli with a halo equal to 22mm. In relation to the tests with the extracts already purified, the fractions from the fruit have more relevant action. The Hexane fraction corresponded to the most expressive results, obtaining halos equal to 28mm, 26mm and 37mm against the strains of E. coli, K. pneumoniae, and S. aureus respectively. As for the tests related to serial dilutions, we observed that after the 1/10 dilution, the extracts no longer had any inhibitory activity on the bacteria under study. However, these results demonstrate the potential of the soursop extract, especially the fruit, as a source of antibacterial compounds. However, it is worthwhile to continue more advanced research with Annona muricata.La resistencia bacteriana a los antibióticos se ha convertido en un grave problema de salud pública en todo el mundo, que afecta a miles de personas. Por tanto, es necesario buscar formas alternativas de combatirlos. Por tanto, esta investigación tuvo como objetivo verificar el efecto antimicrobiano del extracto de Annona muricata L. frente a cepas ATCC de Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa y Staphylococcus aureus. Se recogió el material y se obtuvieron los extractos, luego se prepararon las cepas bacterianas para las pruebas de susceptibilidad. Las pruebas se desarrollaron en tres etapas: extractos crudos, extractos fraccionados químicamente y pruebas con diluciones seriadas. Se observó actividad antibacteriana de guanábana frente a todas las cepas ensayadas, siendo el resultado más significativo el extracto crudo de la fruta frente a la cepa de E. coli con un halo igual a 22 mm. En relación a las pruebas con los extractos ya purificados, las fracciones de la fruta tienen una acción más relevante. La fracción Hexano correspondió a los resultados más expresivos, obteniendo halos iguales a 28 mm, 26 mm y 37 mm frente a las cepas de E. coli, K. pneumoniae y S. aureus respectivamente. En cuanto a las pruebas relacionadas con las diluciones seriadas, observamos que después de la dilución 1/10, los extractos ya no tenían actividad inhibidora sobre las bacterias en estudio. Sin embargo, estos resultados demuestran el potencial del extracto de guanábana, especialmente la fruta, como fuente de compuestos antibacterianos. Sin embargo, vale la pena continuar con investigaciones más avanzadas con Annona muricata.A resistência bacteriana a antibióticos tornou-se mundialmente um sério problema de Saúde Pública atingindo milhares de pessoas. Assim, vê-se a necessidade de buscar formas alternativas no combate das mesmas.  Portanto, essa pesquisa objetivou verificar o efeito antimicrobiano do extrato da Annona muricata L. frente a cepas ATCC de Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa e Staphylococcus aureus. Realizou-se a coleta do material e a obtenção dos extratos, posteriormente houve a preparação das cepas bacterianas para os testes de suscetibilidade. Os testes prosseguiram em três etapas: extratos brutos, extratos fracionados quimicamente e testes com diluições seriadas.  Verificou-se atividade antibacteriana da graviola frente a todas as cepas testas sendo o resultado mais significante referente ao extrato bruto do fruto frente a cepa da E. coli com halo igual a 22mm. Em relação aos testes com os extratos já purificados, as frações provenientes do fruto tiverem ação mais relevantes. A fração de Hexano correspondeu em resultados mais expressivos obtendo halos igual a 28mm, 26mm e 37mm frente as cepas de E. coli, K. pneumoniae, e S. aureus respectivamente. Quanto aos testes relacionados as diluições seriadas observamos que a partir da diluição de 1/10 os extratos já não possuíram nenhuma atividade inibitória sobre as bactérias em estudo. Contudo, esses resultados demonstram o potencial do extrato da graviola, em especial do fruto, como fonte de compostos antibacterianos. No entanto, é válido continuar pesquisas mais avançadas com a Annona muricata.Research, Society and Development2021-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1495010.33448/rsd-v10i5.14950Research, Society and Development; Vol. 10 No. 5; e33510514950Research, Society and Development; Vol. 10 Núm. 5; e33510514950Research, Society and Development; v. 10 n. 5; e335105149502525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/14950/13462Copyright (c) 2021 Anny Karoline Rodrigues Batista; Kellyane Karen Ferreira Aguiar Cesar; Luciana Rocha Paula; Francisco Laurindo da Silva; Haysha Laianne Oliveira Raposohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBatista, Anny Karoline Rodrigues Cesar, Kellyane Karen Ferreira Aguiar Paula, Luciana Rocha Silva, Francisco Laurindo daRaposo, Haysha Laianne Oliveira 2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/14950Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:54.034776Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.
Potencial antibacteriano (in vitro) del extracto metanólico de Annona muricata L.
Potencial antibacteriano (in vitro) do extrato metanólico da Annona muricata L.
title Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.
spellingShingle Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.
Batista, Anny Karoline Rodrigues
Extratos vegetais
Bactérias
Teste de suscetibilidade.
Extractos de plantas
Bacterias
Prueba de susceptibilidad.
Plant extracts
Bacteria
Susceptibility test.
title_short Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.
title_full Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.
title_fullStr Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.
title_full_unstemmed Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.
title_sort Antibacterial potential (in vitro) of methanolic extract from Annona muricata L.
author Batista, Anny Karoline Rodrigues
author_facet Batista, Anny Karoline Rodrigues
Cesar, Kellyane Karen Ferreira Aguiar
Paula, Luciana Rocha
Silva, Francisco Laurindo da
Raposo, Haysha Laianne Oliveira
author_role author
author2 Cesar, Kellyane Karen Ferreira Aguiar
Paula, Luciana Rocha
Silva, Francisco Laurindo da
Raposo, Haysha Laianne Oliveira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Batista, Anny Karoline Rodrigues
Cesar, Kellyane Karen Ferreira Aguiar
Paula, Luciana Rocha
Silva, Francisco Laurindo da
Raposo, Haysha Laianne Oliveira
dc.subject.por.fl_str_mv Extratos vegetais
Bactérias
Teste de suscetibilidade.
Extractos de plantas
Bacterias
Prueba de susceptibilidad.
Plant extracts
Bacteria
Susceptibility test.
topic Extratos vegetais
Bactérias
Teste de suscetibilidade.
Extractos de plantas
Bacterias
Prueba de susceptibilidad.
Plant extracts
Bacteria
Susceptibility test.
description Bacterial resistance to antibiotics has become a serious public health problem worldwide, affecting thousands of people. Thus, there is a need to seek alternative ways to combat them. Therefore, this research aimed to verify the antimicrobial effect of the extract of Annona muricata L. against ATCC strains of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. The material was collected and the extracts were obtained, then the bacterial strains were prepared for susceptibility testing. The tests proceeded in three stages: crude extracts, chemically fractionated extracts and tests with serial dilutions. Soursop antibacterial activity was observed against all strains tested, the most significant result being the crude extract of the fruit against the strain of E. coli with a halo equal to 22mm. In relation to the tests with the extracts already purified, the fractions from the fruit have more relevant action. The Hexane fraction corresponded to the most expressive results, obtaining halos equal to 28mm, 26mm and 37mm against the strains of E. coli, K. pneumoniae, and S. aureus respectively. As for the tests related to serial dilutions, we observed that after the 1/10 dilution, the extracts no longer had any inhibitory activity on the bacteria under study. However, these results demonstrate the potential of the soursop extract, especially the fruit, as a source of antibacterial compounds. However, it is worthwhile to continue more advanced research with Annona muricata.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/14950
10.33448/rsd-v10i5.14950
url https://rsdjournal.org/index.php/rsd/article/view/14950
identifier_str_mv 10.33448/rsd-v10i5.14950
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/14950/13462
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 5; e33510514950
Research, Society and Development; Vol. 10 Núm. 5; e33510514950
Research, Society and Development; v. 10 n. 5; e33510514950
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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