Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/20667 |
Resumo: | This study investigated the action of the hydroalcohol extract Arrabidaea chica (AchE) against C. albicans, as well as its effect on cytotoxicity in fibroblasts and keratinocytes and changes in the physical/mechanical properties of PPMA resin. The susceptibility of C. albicans to AchE was assessed using the MIC and MFC. For the susceptibility test, C. albicans biofilms were developed in PMMA acrylic resin disc for 48 hours and immersed in 5 groups, phosphate-saline buffer, NaoCl 1% or in AchE at CIM, 5xCIM or 10xCIM. The cytotoxicity of AchE (10, 50 and 100 µg /mL) in fibroblasts and keratinocytes was evaluated by MTT, with distilled water and NaOCl l% used as controls. For evaluation of physical/mechanical properties, discs were immersed in the 5 already described groups. The discs were incubated and the testes were performed after 0, 7, 14, 21 and 28 days of immersion. Surface roughness, color change and flexural strength were verified. AchE was considered a strong inhibitor and with bactericidal potential. At 10 µg/mL it did not show cytotoxicity compared to the control group. Other experimental groups were cytotoxic, however, with lower values when compared to NaOCl 1%. There were no significant differences in group-independent physical/mechanical properties or time taken. AchE, when used in high concentrations, was cytotoxic and, regardless of the tools used, it did not interfere with the physical/mechanical properties of PMMA resins. |
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Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resinAcción del extracto de Arrabidaea chica sobre las biopelículas de Candida albicans, efecto citotóxico sobre los fibroblastos y queratinócitos, y sobre las propriedades físico-mecánicas de la resina de poli(metilmetacrilato)Ação do extrato de Arrabidaea chica em biofilmes de Candida albicans, efeito citotóxico em fibroblastos e queratinócitos, e nas propriedades físico/mecânicas de resina de poli(metilmetacrilato) Arrabidaea chica, biopelículas, Candida albicans, citotoxicidad. Fibroblastos, queratinocitos, polimetilmetacrilato.Arrabidaea chicaBiopelículasCandida albicansCitotoxicidadFibroblastosQueratinocitosPolimetilmetacrilato.Arrabidaea chicaArrabidaea chica, biofilmes, Candida albicans, citotoxicidade. Fibroblastos, queratinócitos, polimetilmetacrilato.BiofilmesCandida albicansCitotoxicidadeFibroblastosQueratinócitosPolimetilmetacrilato.Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate.Arrabidaea chicaBiofilmsCandida albicansCytotoxicityFibroblastsKeratinocytesPolymethylmethacrylate.This study investigated the action of the hydroalcohol extract Arrabidaea chica (AchE) against C. albicans, as well as its effect on cytotoxicity in fibroblasts and keratinocytes and changes in the physical/mechanical properties of PPMA resin. The susceptibility of C. albicans to AchE was assessed using the MIC and MFC. For the susceptibility test, C. albicans biofilms were developed in PMMA acrylic resin disc for 48 hours and immersed in 5 groups, phosphate-saline buffer, NaoCl 1% or in AchE at CIM, 5xCIM or 10xCIM. The cytotoxicity of AchE (10, 50 and 100 µg /mL) in fibroblasts and keratinocytes was evaluated by MTT, with distilled water and NaOCl l% used as controls. For evaluation of physical/mechanical properties, discs were immersed in the 5 already described groups. The discs were incubated and the testes were performed after 0, 7, 14, 21 and 28 days of immersion. Surface roughness, color change and flexural strength were verified. AchE was considered a strong inhibitor and with bactericidal potential. At 10 µg/mL it did not show cytotoxicity compared to the control group. Other experimental groups were cytotoxic, however, with lower values when compared to NaOCl 1%. There were no significant differences in group-independent physical/mechanical properties or time taken. AchE, when used in high concentrations, was cytotoxic and, regardless of the tools used, it did not interfere with the physical/mechanical properties of PMMA resins.Este estudio investigó la acción del extracto de hidroalcohol Arrabidaea chica (AchE) contra C. albicans, así como su efecto sobre la citotoxicidad en fibroblastos y queratinocitos y cambios en las propiedades físico-mecánicas de la resina de PMMA. La susceptibilidad de C. albicans a AchE se evaluó utilizando la CIM y CFM. Para la prueba de susceptibilidad, se desarrollaron biopelículas de C. albicans en disco de resina acrílica de PMMA durante 48 horas y se sumergieron en 5 grupos, tampón fosfato-salino, NaOCl 1% o en AchE en CIM, 5xCIM o 10xCIM. La citotoxicidad de AchE (10, 50 y 100 µg/mL) en fibroblastos y queratinocitos se evaluó mediante MTT, con agua destilada y NaOCl 1% como controles. Para la evaluación de las propiedades físicas/mecánicas (rugosidad de la superficie, coloración y resistencia a la flexión), los discos se sumergieron en los 5 grupos ya descritos. Los discos se incubaron y los testes se realizaron a los 0, 7, 14, 21 y 28 días de inmersión. AcHE se consideró un inhibidor fuerte y con potencial bactericida. A 10 µg/mL no mostró citotoxicidad, en comparación con el grupo de control. Otros grupos experimentales fueron citotóxicos, sin embargo, con valores bajos en comparación con NaOCl 1%. No hubo diferencias significativas en las propiedades físicas/mecánicas independientes del grupo o en el tiempo empleado. AchE, cuando se usó en alta concentración, fue citotóxico en células de fibroblastos y queratinocitos y, independientemente de las concentraciones utilizadas, no interfirió con las propiedades físicas/mecánicas de las resinas de PMMA.Este estudo investigou a ação do extrato hidroalcoóde Arrabidaea chica (AchE) contra C. albicans, bem como seu efeito na citotoxicidade em fibroblastos e queratinócitos e alterações nas propriedades físico/mecânicas de resina de PMMA. A susceptibilidade da C. albicans ao AchE foi avaliada por meio da CIM e CFM. Para os testes de suscetibilidade, biofilmes de C. albicans foram desenvolvidos em disco de resina acrílica de PMMA por 48 horas e imersos em 5 grupos, tampão fosfato-salino, hipoclorito de sódio 1% ou em AchE na CIM, 5xCIM ou 10xCIM. A citotoxicidade do AchE (10, 50 e 100 µg/m) em fibroblastos e queratinócitos foi avaliada pelo método MTT sendo a água destilada e NaOCl l% utilizados como controles. Para avaliação das propriedades físico/mecânicas (rugosidade superficial, alteração de cor e resistência flexural), discos foram imersos nos 5 grupos já descritos. Os discos foram incubados e os testes foram realizados após 0, 7, 14, 21 e 28 dias de imersão. O AcHE foi considerado um forte inibidor e com potencial bactericida. A 10 µg/mL não apresentou citotoxicidade, comparando-se ao grupo controle. Demais grupos experimentais foram citotóxicos, porém, com valores menores quando comparados ao NaOCl 1%. Não houve diferenças significativas nas propriedades físico/mecânicas independente do grupo ou tempo avaliados. O AchE, quando utilizado em altas concentrações, foi citotóxico em células fibroblastos e queratinócitos e, independente das concentrações utilizadas, não interferiu nas propriedades físico/mecânicas das resinas PMMA.Research, Society and Development2021-10-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2066710.33448/rsd-v10i13.20667Research, Society and Development; Vol. 10 No. 13; e292101320667Research, Society and Development; Vol. 10 Núm. 13; e292101320667Research, Society and Development; v. 10 n. 13; e2921013206672525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/20667/18917Copyright (c) 2021 Camila Maria Martins Brandão; Viviane Hass; Patricia Wiziack Zago; Lívia Câmara de Carvalho Galvão; Daniela Bassi Dibai; Fabricio Brito Silva; Adriana Santos Malheiros; Paulo Cézar Simamoto Júnior; Rudys Rodolfo de Jesus Tavarezhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBrandão, Camila Maria Martins Hass, Viviane Zago, Patricia Wiziack Galvão, Lívia Câmara de Carvalho Dibai, Daniela Bassi Silva, Fabricio Brito Malheiros, Adriana Santos Simamoto Júnior, Paulo Cézar Tavarez, Rudys Rodolfo de Jesus 2021-11-21T18:26:28Zoai:ojs.pkp.sfu.ca:article/20667Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:40:14.650873Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin Acción del extracto de Arrabidaea chica sobre las biopelículas de Candida albicans, efecto citotóxico sobre los fibroblastos y queratinócitos, y sobre las propriedades físico-mecánicas de la resina de poli(metilmetacrilato) Ação do extrato de Arrabidaea chica em biofilmes de Candida albicans, efeito citotóxico em fibroblastos e queratinócitos, e nas propriedades físico/mecânicas de resina de poli(metilmetacrilato) |
title |
Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin |
spellingShingle |
Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin Brandão, Camila Maria Martins Arrabidaea chica, biopelículas, Candida albicans, citotoxicidad. Fibroblastos, queratinocitos, polimetilmetacrilato. Arrabidaea chica Biopelículas Candida albicans Citotoxicidad Fibroblastos Queratinocitos Polimetilmetacrilato. Arrabidaea chica Arrabidaea chica, biofilmes, Candida albicans, citotoxicidade. Fibroblastos, queratinócitos, polimetilmetacrilato. Biofilmes Candida albicans Citotoxicidade Fibroblastos Queratinócitos Polimetilmetacrilato. Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate. Arrabidaea chica Biofilms Candida albicans Cytotoxicity Fibroblasts Keratinocytes Polymethylmethacrylate. |
title_short |
Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin |
title_full |
Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin |
title_fullStr |
Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin |
title_full_unstemmed |
Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin |
title_sort |
Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin |
author |
Brandão, Camila Maria Martins |
author_facet |
Brandão, Camila Maria Martins Hass, Viviane Zago, Patricia Wiziack Galvão, Lívia Câmara de Carvalho Dibai, Daniela Bassi Silva, Fabricio Brito Malheiros, Adriana Santos Simamoto Júnior, Paulo Cézar Tavarez, Rudys Rodolfo de Jesus |
author_role |
author |
author2 |
Hass, Viviane Zago, Patricia Wiziack Galvão, Lívia Câmara de Carvalho Dibai, Daniela Bassi Silva, Fabricio Brito Malheiros, Adriana Santos Simamoto Júnior, Paulo Cézar Tavarez, Rudys Rodolfo de Jesus |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Brandão, Camila Maria Martins Hass, Viviane Zago, Patricia Wiziack Galvão, Lívia Câmara de Carvalho Dibai, Daniela Bassi Silva, Fabricio Brito Malheiros, Adriana Santos Simamoto Júnior, Paulo Cézar Tavarez, Rudys Rodolfo de Jesus |
dc.subject.por.fl_str_mv |
Arrabidaea chica, biopelículas, Candida albicans, citotoxicidad. Fibroblastos, queratinocitos, polimetilmetacrilato. Arrabidaea chica Biopelículas Candida albicans Citotoxicidad Fibroblastos Queratinocitos Polimetilmetacrilato. Arrabidaea chica Arrabidaea chica, biofilmes, Candida albicans, citotoxicidade. Fibroblastos, queratinócitos, polimetilmetacrilato. Biofilmes Candida albicans Citotoxicidade Fibroblastos Queratinócitos Polimetilmetacrilato. Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate. Arrabidaea chica Biofilms Candida albicans Cytotoxicity Fibroblasts Keratinocytes Polymethylmethacrylate. |
topic |
Arrabidaea chica, biopelículas, Candida albicans, citotoxicidad. Fibroblastos, queratinocitos, polimetilmetacrilato. Arrabidaea chica Biopelículas Candida albicans Citotoxicidad Fibroblastos Queratinocitos Polimetilmetacrilato. Arrabidaea chica Arrabidaea chica, biofilmes, Candida albicans, citotoxicidade. Fibroblastos, queratinócitos, polimetilmetacrilato. Biofilmes Candida albicans Citotoxicidade Fibroblastos Queratinócitos Polimetilmetacrilato. Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate. Arrabidaea chica Biofilms Candida albicans Cytotoxicity Fibroblasts Keratinocytes Polymethylmethacrylate. |
description |
This study investigated the action of the hydroalcohol extract Arrabidaea chica (AchE) against C. albicans, as well as its effect on cytotoxicity in fibroblasts and keratinocytes and changes in the physical/mechanical properties of PPMA resin. The susceptibility of C. albicans to AchE was assessed using the MIC and MFC. For the susceptibility test, C. albicans biofilms were developed in PMMA acrylic resin disc for 48 hours and immersed in 5 groups, phosphate-saline buffer, NaoCl 1% or in AchE at CIM, 5xCIM or 10xCIM. The cytotoxicity of AchE (10, 50 and 100 µg /mL) in fibroblasts and keratinocytes was evaluated by MTT, with distilled water and NaOCl l% used as controls. For evaluation of physical/mechanical properties, discs were immersed in the 5 already described groups. The discs were incubated and the testes were performed after 0, 7, 14, 21 and 28 days of immersion. Surface roughness, color change and flexural strength were verified. AchE was considered a strong inhibitor and with bactericidal potential. At 10 µg/mL it did not show cytotoxicity compared to the control group. Other experimental groups were cytotoxic, however, with lower values when compared to NaOCl 1%. There were no significant differences in group-independent physical/mechanical properties or time taken. AchE, when used in high concentrations, was cytotoxic and, regardless of the tools used, it did not interfere with the physical/mechanical properties of PMMA resins. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/20667 10.33448/rsd-v10i13.20667 |
url |
https://rsdjournal.org/index.php/rsd/article/view/20667 |
identifier_str_mv |
10.33448/rsd-v10i13.20667 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/20667/18917 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 13; e292101320667 Research, Society and Development; Vol. 10 Núm. 13; e292101320667 Research, Society and Development; v. 10 n. 13; e292101320667 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052690684968960 |