Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin

Detalhes bibliográficos
Autor(a) principal: Brandão, Camila Maria Martins
Data de Publicação: 2021
Outros Autores: Hass, Viviane, Zago, Patricia Wiziack, Galvão, Lívia Câmara de Carvalho, Dibai, Daniela Bassi, Silva, Fabricio Brito, Malheiros, Adriana Santos, Simamoto Júnior, Paulo Cézar, Tavarez, Rudys Rodolfo de Jesus
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/20667
Resumo: This study investigated the action of the hydroalcohol extract Arrabidaea chica (AchE) against C. albicans, as well as its effect on cytotoxicity in fibroblasts and keratinocytes and changes in the physical/mechanical properties of PPMA resin. The susceptibility of C. albicans to AchE was assessed using the MIC and MFC. For the susceptibility test, C. albicans biofilms were developed in PMMA acrylic resin disc for 48 hours and immersed in 5 groups, phosphate-saline buffer, NaoCl 1% or in AchE at CIM, 5xCIM or 10xCIM. The cytotoxicity of AchE (10, 50 and 100 µg /mL) in fibroblasts and keratinocytes was evaluated by MTT, with distilled water and NaOCl l% used as controls. For evaluation of physical/mechanical properties, discs were immersed in the 5 already described groups. The discs were incubated and the testes were performed after 0, 7, 14, 21 and 28 days of immersion. Surface roughness, color change and flexural strength were verified. AchE was considered a strong inhibitor and with bactericidal potential. At 10 µg/mL it did not show cytotoxicity compared to the control group. Other experimental groups were cytotoxic, however, with lower values when compared to NaOCl 1%. There were no significant differences in group-independent physical/mechanical properties or time taken. AchE, when used in high concentrations, was cytotoxic and, regardless of the tools used, it did not interfere with the physical/mechanical properties of PMMA resins.
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spelling Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resinAcción del extracto de Arrabidaea chica sobre las biopelículas de Candida albicans, efecto citotóxico sobre los fibroblastos y queratinócitos, y sobre las propriedades físico-mecánicas de la resina de poli(metilmetacrilato)Ação do extrato de Arrabidaea chica em biofilmes de Candida albicans, efeito citotóxico em fibroblastos e queratinócitos, e nas propriedades físico/mecânicas de resina de poli(metilmetacrilato) Arrabidaea chica, biopelículas, Candida albicans, citotoxicidad. Fibroblastos, queratinocitos, polimetilmetacrilato.Arrabidaea chicaBiopelículasCandida albicansCitotoxicidadFibroblastosQueratinocitosPolimetilmetacrilato.Arrabidaea chicaArrabidaea chica, biofilmes, Candida albicans, citotoxicidade. Fibroblastos, queratinócitos, polimetilmetacrilato.BiofilmesCandida albicansCitotoxicidadeFibroblastosQueratinócitosPolimetilmetacrilato.Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate.Arrabidaea chicaBiofilmsCandida albicansCytotoxicityFibroblastsKeratinocytesPolymethylmethacrylate.This study investigated the action of the hydroalcohol extract Arrabidaea chica (AchE) against C. albicans, as well as its effect on cytotoxicity in fibroblasts and keratinocytes and changes in the physical/mechanical properties of PPMA resin. The susceptibility of C. albicans to AchE was assessed using the MIC and MFC. For the susceptibility test, C. albicans biofilms were developed in PMMA acrylic resin disc for 48 hours and immersed in 5 groups, phosphate-saline buffer, NaoCl 1% or in AchE at CIM, 5xCIM or 10xCIM. The cytotoxicity of AchE (10, 50 and 100 µg /mL) in fibroblasts and keratinocytes was evaluated by MTT, with distilled water and NaOCl l% used as controls. For evaluation of physical/mechanical properties, discs were immersed in the 5 already described groups. The discs were incubated and the testes were performed after 0, 7, 14, 21 and 28 days of immersion. Surface roughness, color change and flexural strength were verified. AchE was considered a strong inhibitor and with bactericidal potential. At 10 µg/mL it did not show cytotoxicity compared to the control group. Other experimental groups were cytotoxic, however, with lower values when compared to NaOCl 1%. There were no significant differences in group-independent physical/mechanical properties or time taken. AchE, when used in high concentrations, was cytotoxic and, regardless of the tools used, it did not interfere with the physical/mechanical properties of PMMA resins.Este estudio investigó la acción del extracto de hidroalcohol Arrabidaea chica (AchE) contra C. albicans, así como su efecto sobre la citotoxicidad en fibroblastos y queratinocitos y cambios en las propiedades físico-mecánicas de la resina de PMMA. La susceptibilidad de C. albicans a AchE se evaluó utilizando la CIM y CFM. Para la prueba de susceptibilidad, se desarrollaron biopelículas de C. albicans en disco de resina acrílica de PMMA durante 48 horas y se sumergieron en 5 grupos, tampón fosfato-salino, NaOCl 1% o en AchE en CIM, 5xCIM o 10xCIM. La citotoxicidad de AchE (10, 50 y 100 µg/mL) en fibroblastos y queratinocitos se evaluó mediante MTT, con agua destilada y NaOCl 1% como controles. Para la evaluación de las propiedades físicas/mecánicas (rugosidad de la superficie, coloración y resistencia a la flexión), los discos se sumergieron en los 5 grupos ya descritos. Los discos se incubaron y los testes se realizaron a los 0, 7, 14, 21 y 28 días de inmersión. AcHE se consideró un inhibidor fuerte y con potencial bactericida. A 10 µg/mL no mostró citotoxicidad, en comparación con el grupo de control. Otros grupos experimentales fueron citotóxicos, sin embargo, con valores bajos en comparación con NaOCl 1%. No hubo diferencias significativas en las propiedades físicas/mecánicas independientes del grupo o en el tiempo empleado. AchE, cuando se usó en alta concentración, fue citotóxico en células de fibroblastos y queratinocitos y, independientemente de las concentraciones utilizadas, no interfirió con las propiedades físicas/mecánicas de las resinas de PMMA.Este estudo investigou a ação do extrato hidroalcoóde Arrabidaea chica (AchE) contra C. albicans, bem como seu efeito na citotoxicidade em fibroblastos e queratinócitos e alterações nas propriedades físico/mecânicas de resina de PMMA. A susceptibilidade da C. albicans ao AchE foi avaliada por meio da CIM e CFM. Para os testes de suscetibilidade, biofilmes de C. albicans foram desenvolvidos em disco de resina acrílica de PMMA por 48 horas e imersos em 5 grupos, tampão fosfato-salino, hipoclorito de sódio 1% ou em AchE na CIM, 5xCIM ou 10xCIM. A citotoxicidade do AchE (10, 50 e 100 µg/m) em fibroblastos e queratinócitos foi avaliada pelo método MTT sendo a água destilada e NaOCl l% utilizados como controles. Para avaliação das propriedades físico/mecânicas (rugosidade superficial, alteração de cor e resistência flexural), discos foram imersos nos 5 grupos já descritos. Os discos foram incubados e os testes foram realizados após 0, 7, 14, 21 e 28 dias de imersão. O AcHE foi considerado um forte inibidor e com potencial bactericida. A 10 µg/mL não apresentou citotoxicidade, comparando-se ao grupo controle. Demais grupos experimentais foram citotóxicos, porém, com valores menores quando comparados ao NaOCl 1%. Não houve diferenças significativas nas propriedades físico/mecânicas independente do grupo ou tempo avaliados. O AchE, quando utilizado em altas concentrações, foi citotóxico em células fibroblastos e queratinócitos e, independente das concentrações utilizadas, não interferiu nas propriedades físico/mecânicas das resinas PMMA.Research, Society and Development2021-10-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2066710.33448/rsd-v10i13.20667Research, Society and Development; Vol. 10 No. 13; e292101320667Research, Society and Development; Vol. 10 Núm. 13; e292101320667Research, Society and Development; v. 10 n. 13; e2921013206672525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/20667/18917Copyright (c) 2021 Camila Maria Martins Brandão; Viviane Hass; Patricia Wiziack Zago; Lívia Câmara de Carvalho Galvão; Daniela Bassi Dibai; Fabricio Brito Silva; Adriana Santos Malheiros; Paulo Cézar Simamoto Júnior; Rudys Rodolfo de Jesus Tavarezhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBrandão, Camila Maria Martins Hass, Viviane Zago, Patricia Wiziack Galvão, Lívia Câmara de Carvalho Dibai, Daniela Bassi Silva, Fabricio Brito Malheiros, Adriana Santos Simamoto Júnior, Paulo Cézar Tavarez, Rudys Rodolfo de Jesus 2021-11-21T18:26:28Zoai:ojs.pkp.sfu.ca:article/20667Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:40:14.650873Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
Acción del extracto de Arrabidaea chica sobre las biopelículas de Candida albicans, efecto citotóxico sobre los fibroblastos y queratinócitos, y sobre las propriedades físico-mecánicas de la resina de poli(metilmetacrilato)
Ação do extrato de Arrabidaea chica em biofilmes de Candida albicans, efeito citotóxico em fibroblastos e queratinócitos, e nas propriedades físico/mecânicas de resina de poli(metilmetacrilato)
title Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
spellingShingle Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
Brandão, Camila Maria Martins
Arrabidaea chica, biopelículas, Candida albicans, citotoxicidad. Fibroblastos, queratinocitos, polimetilmetacrilato.
Arrabidaea chica
Biopelículas
Candida albicans
Citotoxicidad
Fibroblastos
Queratinocitos
Polimetilmetacrilato.
Arrabidaea chica
Arrabidaea chica, biofilmes, Candida albicans, citotoxicidade. Fibroblastos, queratinócitos, polimetilmetacrilato.
Biofilmes
Candida albicans
Citotoxicidade
Fibroblastos
Queratinócitos
Polimetilmetacrilato.
Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate.
Arrabidaea chica
Biofilms
Candida albicans
Cytotoxicity
Fibroblasts
Keratinocytes
Polymethylmethacrylate.
title_short Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
title_full Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
title_fullStr Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
title_full_unstemmed Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
title_sort Action of Arrabidaea chica extract on Candida albicans biofilms, cytotoxic effect on fibroblasts and keratinocytes, and on physical/mechanical porperties of poli(methylmethacrylate) resin
author Brandão, Camila Maria Martins
author_facet Brandão, Camila Maria Martins
Hass, Viviane
Zago, Patricia Wiziack
Galvão, Lívia Câmara de Carvalho
Dibai, Daniela Bassi
Silva, Fabricio Brito
Malheiros, Adriana Santos
Simamoto Júnior, Paulo Cézar
Tavarez, Rudys Rodolfo de Jesus
author_role author
author2 Hass, Viviane
Zago, Patricia Wiziack
Galvão, Lívia Câmara de Carvalho
Dibai, Daniela Bassi
Silva, Fabricio Brito
Malheiros, Adriana Santos
Simamoto Júnior, Paulo Cézar
Tavarez, Rudys Rodolfo de Jesus
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brandão, Camila Maria Martins
Hass, Viviane
Zago, Patricia Wiziack
Galvão, Lívia Câmara de Carvalho
Dibai, Daniela Bassi
Silva, Fabricio Brito
Malheiros, Adriana Santos
Simamoto Júnior, Paulo Cézar
Tavarez, Rudys Rodolfo de Jesus
dc.subject.por.fl_str_mv Arrabidaea chica, biopelículas, Candida albicans, citotoxicidad. Fibroblastos, queratinocitos, polimetilmetacrilato.
Arrabidaea chica
Biopelículas
Candida albicans
Citotoxicidad
Fibroblastos
Queratinocitos
Polimetilmetacrilato.
Arrabidaea chica
Arrabidaea chica, biofilmes, Candida albicans, citotoxicidade. Fibroblastos, queratinócitos, polimetilmetacrilato.
Biofilmes
Candida albicans
Citotoxicidade
Fibroblastos
Queratinócitos
Polimetilmetacrilato.
Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate.
Arrabidaea chica
Biofilms
Candida albicans
Cytotoxicity
Fibroblasts
Keratinocytes
Polymethylmethacrylate.
topic Arrabidaea chica, biopelículas, Candida albicans, citotoxicidad. Fibroblastos, queratinocitos, polimetilmetacrilato.
Arrabidaea chica
Biopelículas
Candida albicans
Citotoxicidad
Fibroblastos
Queratinocitos
Polimetilmetacrilato.
Arrabidaea chica
Arrabidaea chica, biofilmes, Candida albicans, citotoxicidade. Fibroblastos, queratinócitos, polimetilmetacrilato.
Biofilmes
Candida albicans
Citotoxicidade
Fibroblastos
Queratinócitos
Polimetilmetacrilato.
Arrabidaea chica, biofilms, Candida albicans, cytotoxicity. Fibroblasts, keratinocytes, polymethylmethacrylate.
Arrabidaea chica
Biofilms
Candida albicans
Cytotoxicity
Fibroblasts
Keratinocytes
Polymethylmethacrylate.
description This study investigated the action of the hydroalcohol extract Arrabidaea chica (AchE) against C. albicans, as well as its effect on cytotoxicity in fibroblasts and keratinocytes and changes in the physical/mechanical properties of PPMA resin. The susceptibility of C. albicans to AchE was assessed using the MIC and MFC. For the susceptibility test, C. albicans biofilms were developed in PMMA acrylic resin disc for 48 hours and immersed in 5 groups, phosphate-saline buffer, NaoCl 1% or in AchE at CIM, 5xCIM or 10xCIM. The cytotoxicity of AchE (10, 50 and 100 µg /mL) in fibroblasts and keratinocytes was evaluated by MTT, with distilled water and NaOCl l% used as controls. For evaluation of physical/mechanical properties, discs were immersed in the 5 already described groups. The discs were incubated and the testes were performed after 0, 7, 14, 21 and 28 days of immersion. Surface roughness, color change and flexural strength were verified. AchE was considered a strong inhibitor and with bactericidal potential. At 10 µg/mL it did not show cytotoxicity compared to the control group. Other experimental groups were cytotoxic, however, with lower values when compared to NaOCl 1%. There were no significant differences in group-independent physical/mechanical properties or time taken. AchE, when used in high concentrations, was cytotoxic and, regardless of the tools used, it did not interfere with the physical/mechanical properties of PMMA resins.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/20667
10.33448/rsd-v10i13.20667
url https://rsdjournal.org/index.php/rsd/article/view/20667
identifier_str_mv 10.33448/rsd-v10i13.20667
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/20667/18917
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 13; e292101320667
Research, Society and Development; Vol. 10 Núm. 13; e292101320667
Research, Society and Development; v. 10 n. 13; e292101320667
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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