Transformation of filamental fungi by Agrobacterium: Histoplasma as a model
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/29427 |
Resumo: | Histoplasma is a pathogenic fungus that causes histoplasmosis, an endemic systemic mycosis due to the occurrence/positivity in specific regions. The virulence of this fungus has been revealed in recent years, aided by the techniques of functional gene characterization, which use gene inactivation, by deletion, or decrease in the production of gene products. Molecular tools for gene transformation, such as Agrobacterium-mediated transformations (ATMT), which favor the formation of RNA interference, have ensured the expansion of knowledge about virulence mechanisms implemented by different pathogenic microorganisms, such as H. capsulatum. In ATMT, a DNA fragment is introduced into the target cell when the bacterium is stimulated by chemical signals. DNA integrates into the genome and provides gene silencing via RNAi, a method of reducing the gene product, due to mRNA degradation, through the formation or introduction of a double-stranded RNA molecule into the target host, which are abhorred by cells eukaryotic. For Histoplasma, the ability of double-stranded RNAs, introduced by the ATMT technique, to trigger depletion of many target genes has been widely demonstrated, and so far has favored a better understanding of the metabolic flexibility of this pathogen. |
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Transformation of filamental fungi by Agrobacterium: Histoplasma as a modelTransformación de hongos filamentales por Agrobacterium: Histoplasma como modeloTransformação de fungos filamentosos por Agrobacterium: O Histoplasma como modeloBiologia molecularSilenciamentoVirulência.Biología molecularSilenciamientoVirulencia.Molecular biologySilencingVirulence.Histoplasma is a pathogenic fungus that causes histoplasmosis, an endemic systemic mycosis due to the occurrence/positivity in specific regions. The virulence of this fungus has been revealed in recent years, aided by the techniques of functional gene characterization, which use gene inactivation, by deletion, or decrease in the production of gene products. Molecular tools for gene transformation, such as Agrobacterium-mediated transformations (ATMT), which favor the formation of RNA interference, have ensured the expansion of knowledge about virulence mechanisms implemented by different pathogenic microorganisms, such as H. capsulatum. In ATMT, a DNA fragment is introduced into the target cell when the bacterium is stimulated by chemical signals. DNA integrates into the genome and provides gene silencing via RNAi, a method of reducing the gene product, due to mRNA degradation, through the formation or introduction of a double-stranded RNA molecule into the target host, which are abhorred by cells eukaryotic. For Histoplasma, the ability of double-stranded RNAs, introduced by the ATMT technique, to trigger depletion of many target genes has been widely demonstrated, and so far has favored a better understanding of the metabolic flexibility of this pathogen.Histoplasma es un hongo patógeno que causa histoplasmosis, una micosis sistémica endémica debido a la aparición/positividad en regiones específicas. La virulencia de este hongo se ha puesto de manifiesto en los últimos años, ayudado por las técnicas de caracterización génica funcional, que utilizan la inactivación génica, por deleción o disminución de la producción de productos génicos. Las herramientas moleculares para la transformación de genes, como las transformaciones mediadas por Agrobacterium (ATMT), que favorecen la formación de RNA de interferencia, han asegurado la ampliación del conocimiento sobre los mecanismos de virulencia implementados por diferentes microorganismos patógenos, como H. capsulatum. En ATMT, un fragmento de ADN se introduce en la célula objetivo cuando la bacteria es estimulada por señales químicas. El DNA se integra en el genoma y proporciona silenciamiento génico mediante RNAi, un método para reducir el producto génico, debido a la degradación del ARNm, mediante la formación o introducción de una molécula de RNA de doble cadena en el huésped objetivo, que las células eucariotas detestan. Para Histoplasma, la capacidad de los RNA de doble cadena, introducidos por la técnica ATMT, para desencadenar el agotamiento de muchos genes diana ya ha sido ampliamente demostrada, y hasta ahora ha favorecido una mejor comprensión de la flexibilidad metabólica de este patógeno.O Histoplasma é um fungo patogênico causador da histoplasmose, uma micose sistêmica endêmica devido à ocorrência/positividade em regiões específicas. A virulência desse fungo tem sido desvendada nos últimos anos auxiliados pelas técnicas de caracterização funcional genica, que utilizam da inativação gênica, por deleção, ou diminuição da produção dos produtos gênicos. Ferramentas moleculares de transformação gênica, como as transformações mediadas por Agrobacterium (ATMT), que favorece a formação de RNA interferência, tem garantido a expansão do conhecimento sobre mecanismos de virulência implementados por diferentes micro-organismos patogênicos, como o H. capsulatum. Na ATMT um fragmento de DNA é introduzido na célula alvo, quando a bactéria é estimulada por sinais químicos. O DNA se integra ao genoma e propicia o silenciamento gênico via RNAi, um método de redução do produto gênico, devido a degradação do mRNA, através da formação ou introdução de uma molécula de RNA de dupla fita no hospedeiro alvo, que são abominados pelas células eucarióticas. Para Histoplasma já foi amplamente demostrada a habilidade dos RNAs de fita dupla, introduzidos pela técnica de ATMT, para desencadear depleção de muitos genes alvo, e favoreceu até o momento a melhor compreensão da flexibilidade metabólica desse patógeno.Research, Society and Development2022-05-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2942710.33448/rsd-v11i7.29427Research, Society and Development; Vol. 11 No. 7; e12211729427Research, Society and Development; Vol. 11 Núm. 7; e12211729427Research, Society and Development; v. 11 n. 7; e122117294272525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/29427/25690Copyright (c) 2022 Dayane Moraeshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMoraes, Dayane2022-06-06T15:12:05Zoai:ojs.pkp.sfu.ca:article/29427Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:46:30.868537Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Transformation of filamental fungi by Agrobacterium: Histoplasma as a model Transformación de hongos filamentales por Agrobacterium: Histoplasma como modelo Transformação de fungos filamentosos por Agrobacterium: O Histoplasma como modelo |
title |
Transformation of filamental fungi by Agrobacterium: Histoplasma as a model |
spellingShingle |
Transformation of filamental fungi by Agrobacterium: Histoplasma as a model Moraes, Dayane Biologia molecular Silenciamento Virulência. Biología molecular Silenciamiento Virulencia. Molecular biology Silencing Virulence. |
title_short |
Transformation of filamental fungi by Agrobacterium: Histoplasma as a model |
title_full |
Transformation of filamental fungi by Agrobacterium: Histoplasma as a model |
title_fullStr |
Transformation of filamental fungi by Agrobacterium: Histoplasma as a model |
title_full_unstemmed |
Transformation of filamental fungi by Agrobacterium: Histoplasma as a model |
title_sort |
Transformation of filamental fungi by Agrobacterium: Histoplasma as a model |
author |
Moraes, Dayane |
author_facet |
Moraes, Dayane |
author_role |
author |
dc.contributor.author.fl_str_mv |
Moraes, Dayane |
dc.subject.por.fl_str_mv |
Biologia molecular Silenciamento Virulência. Biología molecular Silenciamiento Virulencia. Molecular biology Silencing Virulence. |
topic |
Biologia molecular Silenciamento Virulência. Biología molecular Silenciamiento Virulencia. Molecular biology Silencing Virulence. |
description |
Histoplasma is a pathogenic fungus that causes histoplasmosis, an endemic systemic mycosis due to the occurrence/positivity in specific regions. The virulence of this fungus has been revealed in recent years, aided by the techniques of functional gene characterization, which use gene inactivation, by deletion, or decrease in the production of gene products. Molecular tools for gene transformation, such as Agrobacterium-mediated transformations (ATMT), which favor the formation of RNA interference, have ensured the expansion of knowledge about virulence mechanisms implemented by different pathogenic microorganisms, such as H. capsulatum. In ATMT, a DNA fragment is introduced into the target cell when the bacterium is stimulated by chemical signals. DNA integrates into the genome and provides gene silencing via RNAi, a method of reducing the gene product, due to mRNA degradation, through the formation or introduction of a double-stranded RNA molecule into the target host, which are abhorred by cells eukaryotic. For Histoplasma, the ability of double-stranded RNAs, introduced by the ATMT technique, to trigger depletion of many target genes has been widely demonstrated, and so far has favored a better understanding of the metabolic flexibility of this pathogen. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/29427 10.33448/rsd-v11i7.29427 |
url |
https://rsdjournal.org/index.php/rsd/article/view/29427 |
identifier_str_mv |
10.33448/rsd-v11i7.29427 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/29427/25690 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Dayane Moraes https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Dayane Moraes https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 7; e12211729427 Research, Society and Development; Vol. 11 Núm. 7; e12211729427 Research, Society and Development; v. 11 n. 7; e12211729427 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052794885111808 |