Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/22488 |
Resumo: | To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (α-MEM) as a base medium, and to evaluate the effects of Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating Hormone (FSH) on the in vitro development and steroid production of isolated porcine preantral follicles. Porcine secondary follicles were cultured in NCSU23 or α-MEM media for 4 days. Once α-MEM was determined to be the optimal culture medium, secondary follicles were cultured in α-MEM alone or supplemented with FSH (1.5 ng/mL), AMH (50 ng/mL) or the combination of the two hormones. Follicle development was evaluated by measuring follicular growth, morphology and hormone production. There was no difference between the media NCSU23 and α-MEM in terms of follicle survival and growth (P > 0.05). However, at day 2, the antrum formation rate tended to be (P < 0.074) higher in α-MEM than NCSU23. At day 4 of culture, the estradiol and progesterone secretion were higher in α-MEM than NCSU23 (P < 0.01), while the opposite was observed for testosterone (P < 0.01). The addition of AMH and/or FSH did not affect follicular survival and growth. Nevertheless, the secretion of estradiol and progesterone induced by FSH was reduced with AMH (P < 0.01). α-MEM is a more effective base medium than NCSU23 for the in vitro follicular development of pig preantral follicles and AMH reduces the steroid production induced by FSH. |
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Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitroEfecto del medio base, la FSH y la hormona antimülleriana (AMH) solas o en combinación sobre el crecimiento de los folículos preantrales porcinos in vitro Efeito do meio de base, FSH e hormônio anti-Mülleriano (AMH) isoladamente ou em combinação no crescimento de folículos pré-antrais suínos in vitro CultivoEsteroidesFoliculogêneseSuíno.CultivoEsteroidesFoliculogénesisPorcino.CultureEsteroidsFolliculogenesisPorcine.To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (α-MEM) as a base medium, and to evaluate the effects of Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating Hormone (FSH) on the in vitro development and steroid production of isolated porcine preantral follicles. Porcine secondary follicles were cultured in NCSU23 or α-MEM media for 4 days. Once α-MEM was determined to be the optimal culture medium, secondary follicles were cultured in α-MEM alone or supplemented with FSH (1.5 ng/mL), AMH (50 ng/mL) or the combination of the two hormones. Follicle development was evaluated by measuring follicular growth, morphology and hormone production. There was no difference between the media NCSU23 and α-MEM in terms of follicle survival and growth (P > 0.05). However, at day 2, the antrum formation rate tended to be (P < 0.074) higher in α-MEM than NCSU23. At day 4 of culture, the estradiol and progesterone secretion were higher in α-MEM than NCSU23 (P < 0.01), while the opposite was observed for testosterone (P < 0.01). The addition of AMH and/or FSH did not affect follicular survival and growth. Nevertheless, the secretion of estradiol and progesterone induced by FSH was reduced with AMH (P < 0.01). α-MEM is a more effective base medium than NCSU23 for the in vitro follicular development of pig preantral follicles and AMH reduces the steroid production induced by FSH.Comparar la eficiencia del medio 23 de la Universidad Estatal de Carolina del Norte (NCSU23) y el medio esencial alfa mínimo (α-MEM) como medio base y evaluar los efectos de la hormona antimülleriana (AMH) sola o en combinación con el folículo estimulante de la hormona (FSH) sobre el desarrollo in vitro y la producción de esteroides a partir de folículos preantrales porcinos aislados. Se cultivaron folículos secundarios porcinos en medio NCSU23 o α-MEM durante 4 días. Una vez que se determinó que α-MEM era el medio de cultivo ideal, los folículos secundarios se cultivaron en α-MEM solo o suplementados con FSH (1,5 ng/ml), AMH (50 ng/ml) o una combinación de las dos hormonas. El desarrollo folicular se evaluó midiendo el crecimiento folicular, la morfología y la producción de hormonas. No hubo diferencia entre los medios NCSU23 y α-MEM en términos de supervivencia y crecimiento folicular (P > 0,05). Sin embargo, en el día 2, la tasa de formación de antro tendió a ser (P < 0.074) más alta en α-MEM que en NCSU23. En el día 4 de cultivo, la secreción de estradiol y progesterona fue mayor en α-MEM que en NCSU23 (P < 0,01), mientras que se observó lo contrario para la testosterona (P < 0,01). La adición de AMH y/o FSH no afectó la supervivencia y el crecimiento folicular. Sin embargo, la secreción de estradiol y progesterona inducida por FSH se redujo con AMH (P < 0,01). α-MEM es un medio de fondo más eficaz que NCSU23 para el desarrollo folicular in vitro de folículos preantrales porcinos y la AMH reduce la producción de esteroides inducida por FSH.Comparar a eficiência do meio 23 da Universidade Estadual da Carolina do Norte (NCSU23) e do meio alfa essencial mínimo (α-MEM) como meio de base e avaliar os efeitos do hormônio anti-Mülleriano (AMH) sozinho ou em combinação com o hormônio folículo estimulante (FSH) no desenvolvimento in vitro e na produção de esteroides de folículos pré-antrais suínos isolados. Folículos secundários suínos foram cultivados em meio NCSU23 ou α-MEM por 4 dias. Uma vez que α-MEM foi determinado ser o meio de cultivo ideal, os folículos secundários foram cultivados em α-MEM sozinho ou suplementado com FSH (1,5 ng/mL), AMH (50 ng/mL) ou na combinação dos dois hormônios. O desenvolvimento folicular foi avaliado medindo o crescimento folicular, a morfologia e a produção hormonal. Não houve diferença entre os meios NCSU23 e α-MEM em termos de sobrevivência e crescimento folicular (P > 0,05). No entanto, no dia 2, a taxa de formação de antro tendeu a ser (P < 0,074) maior em α-MEM do que NCSU23. No dia 4 de cultivo, a secreção de estradiol e progesterona foram maiores no α-MEM do que no NCSU23 (P < 0,01), enquanto o oposto foi observado para a testosterona (P < 0,01). A adição de AMH e/ou FSH não afetou a sobrevivência e o crescimento folicular. No entanto, a secreção de estradiol e progesterona induzida pelo FSH foi reduzida com o AMH (P < 0,01). α-MEM é um meio de base mais eficaz do que o NCSU23 para o desenvolvimento folicular in vitro de folículos pré-antrais suínos e o AMH reduz a produção de esteroides induzida por FSH.Research, Society and Development2021-11-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2248810.33448/rsd-v10i15.22488Research, Society and Development; Vol. 10 No. 15; e53101522488Research, Society and Development; Vol. 10 Núm. 15; e53101522488Research, Society and Development; v. 10 n. 15; e531015224882525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/22488/19938Copyright (c) 2021 Rebeca Magalhães Pedrosa Rocha; Marcello Rubessa; Laritza Ferreira de Lima; Ana Flávia Bezerra da Silva; Rebecca Winters; Kathryn Polkoff; Derek Milner; Cláudio Cabral Campello; Jose Ricardo de Figueiredo; Matthew Wheelerhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRocha, Rebeca Magalhães Pedrosa Rubessa, Marcello Lima, Laritza Ferreira de Silva, Ana Flávia Bezerra da Winters, RebeccaPolkoff, Kathryn Milner, Derek Campello, Cláudio Cabral Figueiredo, Jose Ricardo deWheeler, Matthew 2021-12-06T10:13:53Zoai:ojs.pkp.sfu.ca:article/22488Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:41:38.540172Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro Efecto del medio base, la FSH y la hormona antimülleriana (AMH) solas o en combinación sobre el crecimiento de los folículos preantrales porcinos in vitro Efeito do meio de base, FSH e hormônio anti-Mülleriano (AMH) isoladamente ou em combinação no crescimento de folículos pré-antrais suínos in vitro |
title |
Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro |
spellingShingle |
Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro Rocha, Rebeca Magalhães Pedrosa Cultivo Esteroides Foliculogênese Suíno. Cultivo Esteroides Foliculogénesis Porcino. Culture Esteroids Folliculogenesis Porcine. |
title_short |
Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro |
title_full |
Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro |
title_fullStr |
Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro |
title_full_unstemmed |
Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro |
title_sort |
Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro |
author |
Rocha, Rebeca Magalhães Pedrosa |
author_facet |
Rocha, Rebeca Magalhães Pedrosa Rubessa, Marcello Lima, Laritza Ferreira de Silva, Ana Flávia Bezerra da Winters, Rebecca Polkoff, Kathryn Milner, Derek Campello, Cláudio Cabral Figueiredo, Jose Ricardo de Wheeler, Matthew |
author_role |
author |
author2 |
Rubessa, Marcello Lima, Laritza Ferreira de Silva, Ana Flávia Bezerra da Winters, Rebecca Polkoff, Kathryn Milner, Derek Campello, Cláudio Cabral Figueiredo, Jose Ricardo de Wheeler, Matthew |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rocha, Rebeca Magalhães Pedrosa Rubessa, Marcello Lima, Laritza Ferreira de Silva, Ana Flávia Bezerra da Winters, Rebecca Polkoff, Kathryn Milner, Derek Campello, Cláudio Cabral Figueiredo, Jose Ricardo de Wheeler, Matthew |
dc.subject.por.fl_str_mv |
Cultivo Esteroides Foliculogênese Suíno. Cultivo Esteroides Foliculogénesis Porcino. Culture Esteroids Folliculogenesis Porcine. |
topic |
Cultivo Esteroides Foliculogênese Suíno. Cultivo Esteroides Foliculogénesis Porcino. Culture Esteroids Folliculogenesis Porcine. |
description |
To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (α-MEM) as a base medium, and to evaluate the effects of Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating Hormone (FSH) on the in vitro development and steroid production of isolated porcine preantral follicles. Porcine secondary follicles were cultured in NCSU23 or α-MEM media for 4 days. Once α-MEM was determined to be the optimal culture medium, secondary follicles were cultured in α-MEM alone or supplemented with FSH (1.5 ng/mL), AMH (50 ng/mL) or the combination of the two hormones. Follicle development was evaluated by measuring follicular growth, morphology and hormone production. There was no difference between the media NCSU23 and α-MEM in terms of follicle survival and growth (P > 0.05). However, at day 2, the antrum formation rate tended to be (P < 0.074) higher in α-MEM than NCSU23. At day 4 of culture, the estradiol and progesterone secretion were higher in α-MEM than NCSU23 (P < 0.01), while the opposite was observed for testosterone (P < 0.01). The addition of AMH and/or FSH did not affect follicular survival and growth. Nevertheless, the secretion of estradiol and progesterone induced by FSH was reduced with AMH (P < 0.01). α-MEM is a more effective base medium than NCSU23 for the in vitro follicular development of pig preantral follicles and AMH reduces the steroid production induced by FSH. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/22488 10.33448/rsd-v10i15.22488 |
url |
https://rsdjournal.org/index.php/rsd/article/view/22488 |
identifier_str_mv |
10.33448/rsd-v10i15.22488 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/22488/19938 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 15; e53101522488 Research, Society and Development; Vol. 10 Núm. 15; e53101522488 Research, Society and Development; v. 10 n. 15; e53101522488 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052695121494016 |