Inga laurina crude extract to control Aedes aegypti
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/9683 |
Resumo: | Aedes aegypti is the um mosquito responsible for the transmission of several diseases to humans such as dengue, chikungunya, Zika virus and urban yellow fever. The control of this culicid is done by utilizing insecticides that cause insect resistance. Therefore, natural alternatives to control A. aegypti have been sought. Thus, the objective of our work was to characterize the chemical composition of the crude extract of the leaves of Inga laurina and to evaluate the insecticidal activity of the extract on the larvae of A. aegypti. The crude extract was prepared form dry leaves by dynamic maceration using alcohol 96º GL as extracting solvent. The chemical identification of the compounds found in the crude extract was done by a gas chromatography coupled to mass spectrometry. The larvicidal activity was determined by larval packet test in third-stage development larvae A. aegypti. Fifteen compounds were identified in the leaves and the major ones were γ- sitosterol (34.39%), phytol (14.51%), squalene (8.57%) and stigmasterol (7.38%). I. laurina leaf crude extract presented larvicidal activity potential against A. aegypti larvae presenting lethal concentration of 50% (CL50) of 0.98 mg/mL and 99% (CL99) of 2.69 mg/mL. Thus, I. laurina leaf crude extract presented rich composition of phytosterols and promising insecticide activity against A. aegypti larvae, offering new possibilities for the application and development of products. |
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Inga laurina crude extract to control Aedes aegypti Extracto crudo de Inga laurina en el control de Aedes aegyptiExtrato bruto de Inga laurina no controle de Aedes aegyptiIngáDengueFitoesterolesγ-sitosterolFitol.IngáDengueFitoesteróisγ- sitosterolFitol.IngáDenguePhytosterolsγ- sitosterolPhytol.Aedes aegypti is the um mosquito responsible for the transmission of several diseases to humans such as dengue, chikungunya, Zika virus and urban yellow fever. The control of this culicid is done by utilizing insecticides that cause insect resistance. Therefore, natural alternatives to control A. aegypti have been sought. Thus, the objective of our work was to characterize the chemical composition of the crude extract of the leaves of Inga laurina and to evaluate the insecticidal activity of the extract on the larvae of A. aegypti. The crude extract was prepared form dry leaves by dynamic maceration using alcohol 96º GL as extracting solvent. The chemical identification of the compounds found in the crude extract was done by a gas chromatography coupled to mass spectrometry. The larvicidal activity was determined by larval packet test in third-stage development larvae A. aegypti. Fifteen compounds were identified in the leaves and the major ones were γ- sitosterol (34.39%), phytol (14.51%), squalene (8.57%) and stigmasterol (7.38%). I. laurina leaf crude extract presented larvicidal activity potential against A. aegypti larvae presenting lethal concentration of 50% (CL50) of 0.98 mg/mL and 99% (CL99) of 2.69 mg/mL. Thus, I. laurina leaf crude extract presented rich composition of phytosterols and promising insecticide activity against A. aegypti larvae, offering new possibilities for the application and development of products.Aedes aegypti es un mosquito responsable de la transmisión de diversas enfermedades al hombre como el dengue, el chikungunya, el virus Zika y la fiebre amarilla urbana. El control de este mosquito se realiza mediante el uso de insecticidas que provocan resistencia a los insectos. Así, se han buscado alternativas naturales para el control de A. aegypti. Así, el objetivo de nuestro trabajo fue caracterizar la composición química del extracto crudo de las hojas de Inga laurina y evaluar la actividad insecticida del extracto sobre las larvas de A. aegypti. El extracto crudo se preparó a partir de las hojas secas mediante la técnica de maceración dinámica utilizando alcohol 96º GL como disolvente de extracción. La identificación química de los compuestos presentes en el extracto crudo se realizó mediante cromatografía de gases acoplada a espectrometría de masas y la actividad larvicida se determinó mediante la prueba de inmersión larvaria en larvas del tercer estadio de desarrollo de A. aegypti. Se identificaron quinze compuestos en las hojas, con γ-sitosterol (34,39%), fitol (14,51%), escualeno (8,57%) y estigmasterol (7,38%) como compuestos principales. El extracto crudo de hojas de I. laurina mostró actividad larvicida potencial contra larvas de A. aegypti, con una concentración letal del 50% (CL50) de 0,98 mg/mL y del 99% (CL99) de 2,69 mg/mL. Por tanto, el extracto crudo de las hojas de I. laurina presentó una composición rica en fitoesteroles y una actividad insecticida prometedora contra las larvas de A. aegypti, ofreciendo nuevas posibilidades para la aplicación y desarrollo de productos.Aedes aegypti é um mosquito responsável pela transmissão de várias doenças para o homem como a dengue, chikungunya, Zika vírus e febre amarela urbana. O controle deste culicídeo é realizado pelo uso de inseticidas os quais causam resistência dos insetos. Assim, tem-se buscado alternativas naturais para o controle de A. aegypti. Deste modo, o objetivo do nosso trabalho foi caracterizar a composição química do extrato bruto das folhas de Inga laurina e avaliar a atividade inseticida do extrato sobre as larvas de A. aegypti. O extrato bruto foi preparado a partir das folhas secas pela técnica de maceração dinâmica utilizando álcool 96º GL como solvente extrator. A identificação química dos compostos presentes no extrato bruto foi realizada por cromatografia em fase gasosa acoplada à espectrometria de massas e atividade larvicida foi determinada pelo teste de imersão larval em larvas do terceiro estágio de desenvolvimento de A. aegypti. Nas folhas foram identificados quinze compostos tendo como majoritários γ- sitosterol (34,39%), fitol (14,51%), esqualeno (8,57%) e estigmasterol (7,38%). O extrato bruto das folhas de I. laurina apresentou potencial atividade larvicida contra as larvas de A. aegypti apresentando concentração letal de 50% (CL50) de 0,98 mg/mL e 99% (CL99) de 2,69 mg/mL. Portanto, o extrato bruto das folhas de I. laurina apresentou rica composição em fitoesteróis e promissora atividade inseticida frente às larvas do A. aegypti oferecendo novas possibilidades para a aplicação e desenvolvimento de produtos.Research, Society and Development2020-11-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/968310.33448/rsd-v9i11.9683Research, Society and Development; Vol. 9 No. 11; e1819119683Research, Society and Development; Vol. 9 Núm. 11; e1819119683Research, Society and Development; v. 9 n. 11; e18191196832525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/9683/8667Copyright (c) 2020 Elisangela Yumi Sugauara; Isabelle Luiz Rahal; Herika Line Marko de Oliveira; Wanessa de Campos Bortolucci; Carla Maria Mariano Fernandez; Maria Graciela Iecher Faria; Suelen Pereira Ruiz; José Eduardo Gonçalves; Nelson Barros Colauto; Zilda Cristiani Gazim; Giani Andrea Lindehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSugauara, Elisangela Yumi Rahal, Isabelle Luiz Oliveira, Herika Line Marko de Bortolucci, Wanessa de Campos Fernandez, Carla Maria MarianoFaria, Maria Graciela Iecher Ruiz, Suelen Pereira Gonçalves, José Eduardo Colauto, Nelson Barros Gazim, Zilda Cristiani Linde, Giani Andrea2020-12-10T23:37:57Zoai:ojs.pkp.sfu.ca:article/9683Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:31:54.246073Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Inga laurina crude extract to control Aedes aegypti Extracto crudo de Inga laurina en el control de Aedes aegypti Extrato bruto de Inga laurina no controle de Aedes aegypti |
| title |
Inga laurina crude extract to control Aedes aegypti |
| spellingShingle |
Inga laurina crude extract to control Aedes aegypti Sugauara, Elisangela Yumi Ingá Dengue Fitoesteroles γ-sitosterol Fitol. Ingá Dengue Fitoesteróis γ- sitosterol Fitol. Ingá Dengue Phytosterols γ- sitosterol Phytol. |
| title_short |
Inga laurina crude extract to control Aedes aegypti |
| title_full |
Inga laurina crude extract to control Aedes aegypti |
| title_fullStr |
Inga laurina crude extract to control Aedes aegypti |
| title_full_unstemmed |
Inga laurina crude extract to control Aedes aegypti |
| title_sort |
Inga laurina crude extract to control Aedes aegypti |
| author |
Sugauara, Elisangela Yumi |
| author_facet |
Sugauara, Elisangela Yumi Rahal, Isabelle Luiz Oliveira, Herika Line Marko de Bortolucci, Wanessa de Campos Fernandez, Carla Maria Mariano Faria, Maria Graciela Iecher Ruiz, Suelen Pereira Gonçalves, José Eduardo Colauto, Nelson Barros Gazim, Zilda Cristiani Linde, Giani Andrea |
| author_role |
author |
| author2 |
Rahal, Isabelle Luiz Oliveira, Herika Line Marko de Bortolucci, Wanessa de Campos Fernandez, Carla Maria Mariano Faria, Maria Graciela Iecher Ruiz, Suelen Pereira Gonçalves, José Eduardo Colauto, Nelson Barros Gazim, Zilda Cristiani Linde, Giani Andrea |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sugauara, Elisangela Yumi Rahal, Isabelle Luiz Oliveira, Herika Line Marko de Bortolucci, Wanessa de Campos Fernandez, Carla Maria Mariano Faria, Maria Graciela Iecher Ruiz, Suelen Pereira Gonçalves, José Eduardo Colauto, Nelson Barros Gazim, Zilda Cristiani Linde, Giani Andrea |
| dc.subject.por.fl_str_mv |
Ingá Dengue Fitoesteroles γ-sitosterol Fitol. Ingá Dengue Fitoesteróis γ- sitosterol Fitol. Ingá Dengue Phytosterols γ- sitosterol Phytol. |
| topic |
Ingá Dengue Fitoesteroles γ-sitosterol Fitol. Ingá Dengue Fitoesteróis γ- sitosterol Fitol. Ingá Dengue Phytosterols γ- sitosterol Phytol. |
| description |
Aedes aegypti is the um mosquito responsible for the transmission of several diseases to humans such as dengue, chikungunya, Zika virus and urban yellow fever. The control of this culicid is done by utilizing insecticides that cause insect resistance. Therefore, natural alternatives to control A. aegypti have been sought. Thus, the objective of our work was to characterize the chemical composition of the crude extract of the leaves of Inga laurina and to evaluate the insecticidal activity of the extract on the larvae of A. aegypti. The crude extract was prepared form dry leaves by dynamic maceration using alcohol 96º GL as extracting solvent. The chemical identification of the compounds found in the crude extract was done by a gas chromatography coupled to mass spectrometry. The larvicidal activity was determined by larval packet test in third-stage development larvae A. aegypti. Fifteen compounds were identified in the leaves and the major ones were γ- sitosterol (34.39%), phytol (14.51%), squalene (8.57%) and stigmasterol (7.38%). I. laurina leaf crude extract presented larvicidal activity potential against A. aegypti larvae presenting lethal concentration of 50% (CL50) of 0.98 mg/mL and 99% (CL99) of 2.69 mg/mL. Thus, I. laurina leaf crude extract presented rich composition of phytosterols and promising insecticide activity against A. aegypti larvae, offering new possibilities for the application and development of products. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-11-11 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9683 10.33448/rsd-v9i11.9683 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/9683 |
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10.33448/rsd-v9i11.9683 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/9683/8667 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 9 No. 11; e1819119683 Research, Society and Development; Vol. 9 Núm. 11; e1819119683 Research, Society and Development; v. 9 n. 11; e1819119683 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052662788653056 |