Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/14249 |
Resumo: | Aim: In vitro evaluation of the influence of bovine xenogenic biomaterials on stem cells from human exfoliated deciduous teeth (SHEDs). The study was divided into three groups: 1) group C (control), containing only MSCs; 2) group BP, containing MSCs and Bonefill Porous®; 3) group BO, containing MSCs and Bio-Oss®. MSCs were derived from a deciduous tooth from a 7-year-old male donor. An aliquot of cells was subjected to immunophenotyping by flow cytometry. Cell viability (neutral red), cytotoxicity (MTT), and cell proliferation (crystal violet) assays were performed. All groups underwent morphological analysis by light microscopy (LM), and the biomaterial with superior performance was submitted to evaluation by scanning electron microscopy (SEM). Time points of 24, 48, and 72 h of culture were used. All results were evaluated with a significance level of 0.05. Results showed that both biomaterials maintained cell viability and cytotoxicity similar to the control. The BO group showed smaller cell proliferation compared to the other groups. In LM evaluation, the BP group showed more spread and adherent cells than the BO group. In SEM, cells of the BP group showed characteristics of more active cells than those of the control. Bovine xenogenic biomaterials positively influenced SHEDs, while the BP group seemed to present higher potential with SHEDs for future application within in vivo and/or clinical studies. |
id |
UNIFEI_78a36b01cdbd04de2560fc6307f4de24 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/14249 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot studyLos biomateriales xenógenos de injertos óseos no interfieren con la viabilidad y proliferación de células madre de dientes caducifolios exfoliados humanos - un estudio piloto in vitroBiomateriais de enxerto ósseo xenogênico não interferem na viabilidade e proliferação de células-tronco de dentes decíduos esfoliados humanos - um estudo piloto in vitroCélulas madreBiomaterialesPulpa dental.Células-troncoBiomateriaisPolpa dentária.Stem cellsBiomaterialsDental pulp.Aim: In vitro evaluation of the influence of bovine xenogenic biomaterials on stem cells from human exfoliated deciduous teeth (SHEDs). The study was divided into three groups: 1) group C (control), containing only MSCs; 2) group BP, containing MSCs and Bonefill Porous®; 3) group BO, containing MSCs and Bio-Oss®. MSCs were derived from a deciduous tooth from a 7-year-old male donor. An aliquot of cells was subjected to immunophenotyping by flow cytometry. Cell viability (neutral red), cytotoxicity (MTT), and cell proliferation (crystal violet) assays were performed. All groups underwent morphological analysis by light microscopy (LM), and the biomaterial with superior performance was submitted to evaluation by scanning electron microscopy (SEM). Time points of 24, 48, and 72 h of culture were used. All results were evaluated with a significance level of 0.05. Results showed that both biomaterials maintained cell viability and cytotoxicity similar to the control. The BO group showed smaller cell proliferation compared to the other groups. In LM evaluation, the BP group showed more spread and adherent cells than the BO group. In SEM, cells of the BP group showed characteristics of more active cells than those of the control. Bovine xenogenic biomaterials positively influenced SHEDs, while the BP group seemed to present higher potential with SHEDs for future application within in vivo and/or clinical studies.Objetivo: Evaluación in vitro de la influencia de los biomateriales xenógenos bovinos en células madre de dientes caducifolios exfoliados (SHEDs) humanos. El estudio se dividió en tres grupos: 1) grupo C (control), que contiene sólo MSCs; 2) grupo BP, que contiene MSCs y Bonefill Porous®; 3) Grupo BO, que contiene MSCs y Bio-Oss®. Los MSC se derivaron de un diente caducifolio de un donante de 7 años. Un conjunto celular fue sometido a inmunofennotización por citometría de flujo. Se realizaron pruebas de viabilidad celular (rojo neutro), citotoxicidad (TM) y proliferación celular (cristal violeta). Todos los grupos fueron sometidos a análisis morfológicos por microscopía ligera (ML), y el biomaterial con rendimiento superior fue sometido a evaluación mediante microscopía electrónica de barrido (SEM). Se utilizaron puntos de tiempo de 24, 48 y 72 horas de cultura. Todos los resultados fueron evaluados con un nivel de significancia de 0,05. Los resultados mostraron que ambos biomateriales mantenían la viabilidad celular y la citotoxicidad similar al control. El grupo bo presentó una menor proliferación celular en comparación con los otros grupos. En la evaluación lm, el grupo BP presentó células más difundidas y adherentes que el grupo bo. En SEM, las células del grupo BP presentaron más características celulares activas que las del control. Los biomateriales xenógenos bovinos influyeron positivamente en los SHEDs, mientras que el grupo BP parecía presentar un mayor potencial con SHEDs para futuras aplicaciones en estudios clínicos y/o in vivo.Objetivo: Avaliação in vitro da influência de biomateriais xenogênicos bovinos sobre células-tronco de dentes decíduos esfoliados humanos (SHEDs). O estudo foi dividido em três grupos: 1) grupo C (controle), contendo apenas CTMs; 2) grupo BP, contendo MSCs e Bonefill Porous®; 3) grupo BO, contendo MSCs e Bio-Oss®. As CTMs foram derivadas de um dente decíduo de um doador de 7 anos de idade. Uma alíquota de células foi submetida à imunofenotipagem por citometria de fluxo. Foram realizados ensaios de viabilidade celular (vermelho neutro), citotoxicidade (MTT) e proliferação celular (cristal violeta). Todos os grupos foram submetidos à análise morfológica por microscopia de luz (ML), e o biomaterial com desempenho superior foi submetido à avaliação por microscopia eletrônica de varredura (MEV). Foram utilizados pontos temporais de 24, 48 e 72 horas de cultura. Todos os resultados foram avaliados com nível de significância de 0,05. Os resultados mostraram que ambos os biomateriais mantiveram a viabilidade celular e citotoxicidade semelhantes ao controle. O grupo BO apresentou proliferação celular menor em comparação aos demais grupos. Na avaliação LM, o grupo BP apresentou mais células disseminadas e aderentes do que o grupo BO. No MEV, as células do grupo BP apresentaram características de células mais ativas do que as do controle. Biomateriais xenogênicos bovinos influenciaram positivamente os SHEDs, enquanto o grupo BP pareceu apresentar maior potencial com SHEDs para futura aplicação em estudos in vivo e / ou clínicos.Research, Society and Development2021-04-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1424910.33448/rsd-v10i4.14249Research, Society and Development; Vol. 10 No. 4; e34410414249Research, Society and Development; Vol. 10 Núm. 4; e34410414249Research, Society and Development; v. 10 n. 4; e344104142492525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/14249/15820Copyright (c) 2021 Jeferson Luis de Oliveira Stroparo; Suyany Gabriely Weiss; Sabrina Cunha da Fonseca; Lisley Janowski Spisila; Carla Castiglia Gonzaga; Gabriel Camargo de Oliveira; Gabriela Loewen Brotto ; Alice Maria Swiech; Eduardo Discher Vieira; Roberto da Rocha Leão Neto;Célia Regina Cavichiolo Franco; Moira Pedroso Leão; Tatiana Miranda Deliberador; Marilisa Carneiro Leão Gabardo; João César Zielakhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessStroparo, Jeferson Luis de OliveiraWeiss, Suyany Gabriely Fonseca, Sabrina Cunha da Spisila, Lisley Janowski Gonzaga, Carla Castiglia Oliveira, Gabriel Camargo de Brotto , Gabriela Loewen Swiech, Alice Maria Vieira, Eduardo DischerLeão Neto, Roberto da RochaFranco, Célia Regina Cavichiolo Leão, Moira PedrosoDeliberador, Tatiana Miranda Gabardo, Marilisa Carneiro Leão Zielak, João César 2021-04-25T11:21:26Zoai:ojs.pkp.sfu.ca:article/14249Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:25.081614Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study Los biomateriales xenógenos de injertos óseos no interfieren con la viabilidad y proliferación de células madre de dientes caducifolios exfoliados humanos - un estudio piloto in vitro Biomateriais de enxerto ósseo xenogênico não interferem na viabilidade e proliferação de células-tronco de dentes decíduos esfoliados humanos - um estudo piloto in vitro |
title |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study |
spellingShingle |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study Stroparo, Jeferson Luis de Oliveira Células madre Biomateriales Pulpa dental. Células-tronco Biomateriais Polpa dentária. Stem cells Biomaterials Dental pulp. |
title_short |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study |
title_full |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study |
title_fullStr |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study |
title_full_unstemmed |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study |
title_sort |
Xenogenic bone grafting biomaterials do not interfere in the viability and proliferation of stem cells from human exfoliated deciduous teeth - an in vitro pilot study |
author |
Stroparo, Jeferson Luis de Oliveira |
author_facet |
Stroparo, Jeferson Luis de Oliveira Weiss, Suyany Gabriely Fonseca, Sabrina Cunha da Spisila, Lisley Janowski Gonzaga, Carla Castiglia Oliveira, Gabriel Camargo de Brotto , Gabriela Loewen Swiech, Alice Maria Vieira, Eduardo Discher Leão Neto, Roberto da Rocha Franco, Célia Regina Cavichiolo Leão, Moira Pedroso Deliberador, Tatiana Miranda Gabardo, Marilisa Carneiro Leão Zielak, João César |
author_role |
author |
author2 |
Weiss, Suyany Gabriely Fonseca, Sabrina Cunha da Spisila, Lisley Janowski Gonzaga, Carla Castiglia Oliveira, Gabriel Camargo de Brotto , Gabriela Loewen Swiech, Alice Maria Vieira, Eduardo Discher Leão Neto, Roberto da Rocha Franco, Célia Regina Cavichiolo Leão, Moira Pedroso Deliberador, Tatiana Miranda Gabardo, Marilisa Carneiro Leão Zielak, João César |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Stroparo, Jeferson Luis de Oliveira Weiss, Suyany Gabriely Fonseca, Sabrina Cunha da Spisila, Lisley Janowski Gonzaga, Carla Castiglia Oliveira, Gabriel Camargo de Brotto , Gabriela Loewen Swiech, Alice Maria Vieira, Eduardo Discher Leão Neto, Roberto da Rocha Franco, Célia Regina Cavichiolo Leão, Moira Pedroso Deliberador, Tatiana Miranda Gabardo, Marilisa Carneiro Leão Zielak, João César |
dc.subject.por.fl_str_mv |
Células madre Biomateriales Pulpa dental. Células-tronco Biomateriais Polpa dentária. Stem cells Biomaterials Dental pulp. |
topic |
Células madre Biomateriales Pulpa dental. Células-tronco Biomateriais Polpa dentária. Stem cells Biomaterials Dental pulp. |
description |
Aim: In vitro evaluation of the influence of bovine xenogenic biomaterials on stem cells from human exfoliated deciduous teeth (SHEDs). The study was divided into three groups: 1) group C (control), containing only MSCs; 2) group BP, containing MSCs and Bonefill Porous®; 3) group BO, containing MSCs and Bio-Oss®. MSCs were derived from a deciduous tooth from a 7-year-old male donor. An aliquot of cells was subjected to immunophenotyping by flow cytometry. Cell viability (neutral red), cytotoxicity (MTT), and cell proliferation (crystal violet) assays were performed. All groups underwent morphological analysis by light microscopy (LM), and the biomaterial with superior performance was submitted to evaluation by scanning electron microscopy (SEM). Time points of 24, 48, and 72 h of culture were used. All results were evaluated with a significance level of 0.05. Results showed that both biomaterials maintained cell viability and cytotoxicity similar to the control. The BO group showed smaller cell proliferation compared to the other groups. In LM evaluation, the BP group showed more spread and adherent cells than the BO group. In SEM, cells of the BP group showed characteristics of more active cells than those of the control. Bovine xenogenic biomaterials positively influenced SHEDs, while the BP group seemed to present higher potential with SHEDs for future application within in vivo and/or clinical studies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14249 10.33448/rsd-v10i4.14249 |
url |
https://rsdjournal.org/index.php/rsd/article/view/14249 |
identifier_str_mv |
10.33448/rsd-v10i4.14249 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14249/15820 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 4; e34410414249 Research, Society and Development; Vol. 10 Núm. 4; e34410414249 Research, Society and Development; v. 10 n. 4; e34410414249 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052747879546880 |