Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/25611 |
Resumo: | Cancer is an important cause of lethality, and melanoma is associated with less than 10% of survival. The traditional treatment includes the use of vinblastine, and it is associated with side effects. Curcumin is extracted from Curcuma longa rhizomes, and studied in many diseases, producing a variety of effects. We investigated the role of various cellular pathways concerning apoptosis, cell cycle enzymes in melanoma cell line SK-MEL-28, after treatment with curcumin, vinblastine, or a combination of both, for 24 hours. After this, we performed cell cycle, apoptosis, wound healing assay, comet assay on cells, and evaluated nitrite accumulation (nitric oxide (NO•) byproduct). Curcumin increased cells in apoptosis and reduced the number of cells in the G1 phase. Vinblastine increased the production of nitrite, and cells in early apoptosis, mainly through the inducement of DNA damage. Cell migration was impaired in all tested groups. In conclusion, curcumin impaired migration, producing NO•, and promoting apoptosis of tumoral cells. Vinblastine also impaired cell migration and increased levels of NO•. Curcumin might be included as an adjuvant in the treatment of melanoma, and help treatment of melanoma, and further studies are needed, especially regarding the synergistic effect of curcumin and vinblastine in the treatment. |
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Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cellsLa curcumina y la vinblastina inducen la apoptosis y alteran la migración en células de melanoma cutáneo humanoCurcumina e vimblastina causam apoptose e prejudicam a migração celular em células de melanoma cutâneo humanoMelanomaCurcuminApoptosisMigration.MelanomaCurcuminaApoptosisMigración.MelanomaCurcuminaApoptoseMigração.Cancer is an important cause of lethality, and melanoma is associated with less than 10% of survival. The traditional treatment includes the use of vinblastine, and it is associated with side effects. Curcumin is extracted from Curcuma longa rhizomes, and studied in many diseases, producing a variety of effects. We investigated the role of various cellular pathways concerning apoptosis, cell cycle enzymes in melanoma cell line SK-MEL-28, after treatment with curcumin, vinblastine, or a combination of both, for 24 hours. After this, we performed cell cycle, apoptosis, wound healing assay, comet assay on cells, and evaluated nitrite accumulation (nitric oxide (NO•) byproduct). Curcumin increased cells in apoptosis and reduced the number of cells in the G1 phase. Vinblastine increased the production of nitrite, and cells in early apoptosis, mainly through the inducement of DNA damage. Cell migration was impaired in all tested groups. In conclusion, curcumin impaired migration, producing NO•, and promoting apoptosis of tumoral cells. Vinblastine also impaired cell migration and increased levels of NO•. Curcumin might be included as an adjuvant in the treatment of melanoma, and help treatment of melanoma, and further studies are needed, especially regarding the synergistic effect of curcumin and vinblastine in the treatment.El cáncer es una causa importante de letalidad y el melanoma se asocia con menos del 10% de supervivencia. El tratamiento tradicional incluye el uso de vinblastina y se asocia con efectos secundarios. La curcumina se extrae de los rizomas de Curcuma longa y se estudia en muchas enfermedades, produciendo una variedad de efectos. Investigamos el papel de varias vías celulares relacionadas con la apoptosis, las enzimas del ciclo celular en la línea celular de melanoma SK-MEL-28, después del tratamiento con curcumina, vinblastina o una combinación de ambos, durante 24 horas. Después de esto, realizamos el ciclo celular, la apoptosis, el ensayo de cicatrización de heridas, el ensayo de cometa en las células y evaluamos la acumulación de nitrito (subproducto de óxido nítrico (NO•)). La curcumina aumentó las células en apoptosis y redujo el número de células en la fase G1. La vinblastina aumentó la producción de nitrito y células en apoptosis temprana, principalmente a través de la inducción de daño en el ADN. La migración celular se vio afectada en todos los grupos analizados. En conclusión, la curcumina perjudicó la migración, produciendo NO• y promoviendo la apoptosis de las células tumorales. La vinblastina también perjudicó la migración celular y aumentó los niveles de NO•. La curcumina podría incluirse como adyuvante en el tratamiento del melanoma y ayudar al tratamiento del melanoma, y se necesitan más estudios, especialmente en relación con el efecto sinérgico de la curcumina y la vinblastina en el tratamiento. O câncer é uma importante causa de letalidade, e o melanoma está associado a menos de 10% de sobrevida. O tratamento tradicional inclui o uso de vimblastina e está associado a diversos efeitos colaterais. A curcumina é uma substância extraída dos rizomas da planta Curcuma longa, e estudada em muitas doenças, produzindo uma ampla variedade de efeitos. Nós investigamos o papel de várias vias celulares relacionadas à apoptose, enzimas do ciclo celular na linhagem celular de melanoma SK-MEL-28, após tratamento com curcumina, vimblastina ou uma combinação de ambos, por 24 horas. Após isso, avaliamos ciclo celular, apoptose, migração celular, ensaio cometa nas e avaliamos o acúmulo de nitrito (subproduto de óxido nítrico (NO•). A curcumina aumentou as células em apoptose e reduziu o número de células na fase G1. A vimblastina, por sua vez, aumentou a produção de nitrito e células em apoptose precoce, principalmente através da indução de danos no DNA. A migração celular foi prejudicada em todos os grupos testados. Em conclusão, a curcumina prejudicou a migração, produzindo NO• e promovendo a apoptose de células tumorais. Assim, A vimblastina também prejudicou a migração celular e aumentou os níveis de NO•. Assim, a curcumina pode ser incluída como adjuvante no tratamento do melanoma e auxiliar no tratamento do melanoma, sendo necessários mais estudos, principalmente quanto ao efeito sinérgico da curcumina e da vimblastina no tratamento.Research, Society and Development2022-01-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2561110.33448/rsd-v11i2.25611Research, Society and Development; Vol. 11 No. 2; e20511225611Research, Society and Development; Vol. 11 Núm. 2; e20511225611Research, Society and Development; v. 11 n. 2; e205112256112525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/25611/22465Copyright (c) 2022 Vinícius Leobet Lunkes; Taís Vidal Palma; Vitor Bastianello Mostardeiro; Moisés Henrique Mastella; Charles Elias Assmann; Micheli Mainardi Pillat; Ivana Beatrice Mânica da Cruz; Vera Maria Melchiors Morsch; Maria Rosa Chitolina; Cinthia Melazzo de Andradehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLunkes, Vinícius LeobetPalma, Taís VidalMostardeiro, Vitor BastianelloMastella, Moisés HenriqueAssmann, Charles EliasPillat, Micheli MainardiCruz, Ivana Beatrice Mânica daMorsch, Vera Maria MelchiorsChitolina, Maria RosaAndrade, Cinthia Melazzo de2022-02-07T01:42:50Zoai:ojs.pkp.sfu.ca:article/25611Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:43:51.443489Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells La curcumina y la vinblastina inducen la apoptosis y alteran la migración en células de melanoma cutáneo humano Curcumina e vimblastina causam apoptose e prejudicam a migração celular em células de melanoma cutâneo humano |
| title |
Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells |
| spellingShingle |
Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells Lunkes, Vinícius Leobet Melanoma Curcumin Apoptosis Migration. Melanoma Curcumina Apoptosis Migración. Melanoma Curcumina Apoptose Migração. |
| title_short |
Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells |
| title_full |
Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells |
| title_fullStr |
Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells |
| title_full_unstemmed |
Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells |
| title_sort |
Curcumin and vinblastine induce apoptosis and impair migration in human cutaneous melanoma cells |
| author |
Lunkes, Vinícius Leobet |
| author_facet |
Lunkes, Vinícius Leobet Palma, Taís Vidal Mostardeiro, Vitor Bastianello Mastella, Moisés Henrique Assmann, Charles Elias Pillat, Micheli Mainardi Cruz, Ivana Beatrice Mânica da Morsch, Vera Maria Melchiors Chitolina, Maria Rosa Andrade, Cinthia Melazzo de |
| author_role |
author |
| author2 |
Palma, Taís Vidal Mostardeiro, Vitor Bastianello Mastella, Moisés Henrique Assmann, Charles Elias Pillat, Micheli Mainardi Cruz, Ivana Beatrice Mânica da Morsch, Vera Maria Melchiors Chitolina, Maria Rosa Andrade, Cinthia Melazzo de |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lunkes, Vinícius Leobet Palma, Taís Vidal Mostardeiro, Vitor Bastianello Mastella, Moisés Henrique Assmann, Charles Elias Pillat, Micheli Mainardi Cruz, Ivana Beatrice Mânica da Morsch, Vera Maria Melchiors Chitolina, Maria Rosa Andrade, Cinthia Melazzo de |
| dc.subject.por.fl_str_mv |
Melanoma Curcumin Apoptosis Migration. Melanoma Curcumina Apoptosis Migración. Melanoma Curcumina Apoptose Migração. |
| topic |
Melanoma Curcumin Apoptosis Migration. Melanoma Curcumina Apoptosis Migración. Melanoma Curcumina Apoptose Migração. |
| description |
Cancer is an important cause of lethality, and melanoma is associated with less than 10% of survival. The traditional treatment includes the use of vinblastine, and it is associated with side effects. Curcumin is extracted from Curcuma longa rhizomes, and studied in many diseases, producing a variety of effects. We investigated the role of various cellular pathways concerning apoptosis, cell cycle enzymes in melanoma cell line SK-MEL-28, after treatment with curcumin, vinblastine, or a combination of both, for 24 hours. After this, we performed cell cycle, apoptosis, wound healing assay, comet assay on cells, and evaluated nitrite accumulation (nitric oxide (NO•) byproduct). Curcumin increased cells in apoptosis and reduced the number of cells in the G1 phase. Vinblastine increased the production of nitrite, and cells in early apoptosis, mainly through the inducement of DNA damage. Cell migration was impaired in all tested groups. In conclusion, curcumin impaired migration, producing NO•, and promoting apoptosis of tumoral cells. Vinblastine also impaired cell migration and increased levels of NO•. Curcumin might be included as an adjuvant in the treatment of melanoma, and help treatment of melanoma, and further studies are needed, especially regarding the synergistic effect of curcumin and vinblastine in the treatment. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-01-23 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/25611 10.33448/rsd-v11i2.25611 |
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https://rsdjournal.org/index.php/rsd/article/view/25611 |
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10.33448/rsd-v11i2.25611 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://rsdjournal.org/index.php/rsd/article/view/25611/22465 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 11 No. 2; e20511225611 Research, Society and Development; Vol. 11 Núm. 2; e20511225611 Research, Society and Development; v. 11 n. 2; e20511225611 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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