Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/12554 |
Resumo: | Avocado (Persea americana) is a fruit crop of economic importance in Ecuador. Currently, a low incidence of Phytophthora cinnamomi has been reported, however, there are other soil pathogens that can affect this crop, even at the initial stages of plant multiplication (nursery), for this reason the use of rootstocks that tolerate these biotic adversities is recommended. In this research, the fungus Fusarium oxysporum was isolated from roots of nursery seedlings with symptoms of necrosis. In addition, an isolate of this pathogenic fungus modified with a strain of Agrobacterium tumefaciens was used to determine the infection of F. oxysporum in the roots of the Fuerte (commercial) and Criollo (local) cultivars. The results allowed to infer that the cultivar Criollo presented a greater tolerance to F. oxysporum than the cultivar Fuerte, which corroborates its use as a rootstock for commercial avocado varieties. Furthermore, to our knowledge, this is the first report of F. oxysporum affecting avocado nursery seedlings in Ecuador. |
id |
UNIFEI_802334c42708bfadb15d632a93c9b69a |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/12554 |
network_acronym_str |
UNIFEI |
network_name_str |
Research, Society and Development |
repository_id_str |
|
spelling |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivarsColonización de Fusarium oxysporum transformado con el gen de la proteína de fluorescencia roja (tdTomato) mediado por Agrobacterium tumefaciens en raíces de dos cultivares de aguacateColonização de Fusarium oxysporum transformada com o gene da proteína fluorescente vermelha (tdTomato) mediada por Agrobacterium tumefaciens em raízes de dois cultivares de abacateBiotecnología agrícolaCriolloFuerteHigromicina BMicroscopia de fluorescenciaPorta-enxerto.Agricultural biothecnologyCriolloFuerteFluorescense microscopyHygromycin BRootstock.Biotecnología agrícolaCriolloFuerteHigromicina BMicroscopía de fluorescenciaPortainjerto. Avocado (Persea americana) is a fruit crop of economic importance in Ecuador. Currently, a low incidence of Phytophthora cinnamomi has been reported, however, there are other soil pathogens that can affect this crop, even at the initial stages of plant multiplication (nursery), for this reason the use of rootstocks that tolerate these biotic adversities is recommended. In this research, the fungus Fusarium oxysporum was isolated from roots of nursery seedlings with symptoms of necrosis. In addition, an isolate of this pathogenic fungus modified with a strain of Agrobacterium tumefaciens was used to determine the infection of F. oxysporum in the roots of the Fuerte (commercial) and Criollo (local) cultivars. The results allowed to infer that the cultivar Criollo presented a greater tolerance to F. oxysporum than the cultivar Fuerte, which corroborates its use as a rootstock for commercial avocado varieties. Furthermore, to our knowledge, this is the first report of F. oxysporum affecting avocado nursery seedlings in Ecuador.El aguacate (Persea americana) es un frutal de importancia económica en el Ecuador. Actualmente se ha reportado una baja incidencia de Phytophthora cinnamomi, sin embargo, existen otros patógenos del suelo que afectan a este cultivo, incluso en etapas iniciales de multiplicación de la planta, por esta razón el uso de portainjertos que toleren estas adversidades bióticas es recomendado. En esta investigación, se aisló el hongo Fusarium oxysporum de plántulas de vivero de raíces con sintomatología de necrosis. Además, se utilizó un aislado de este hongo patógeno modificada con una cepa de Agrobacterium tumefaciens para determinar la infección de F. oxysporum en las raíces de los cultivares Fuerte y Criollo. Los resultados permiten inferir que el cultivar Criollo presentó una mayor tolerancia a F. oxysporum, con lo cual se corrobora su uso como portainjerto para variedades comerciales de aguacate. Además, de acuerdo a nuestro conocimiento, este es el primer reporte de F. oxysporum afectando a plántulas de aguacate.O abacate (Persea americana) é uma árvore frutífera de importância econômica no Equador. Atualmente, tem sido relatada uma baixa incidência de Phytophthora cinnamomi, entretanto, existem outros patógenos de solo que afetam esta cultura, mesmo nos estágios iniciais de multiplicação das plantas, por isso é recomendado o uso de porta-enxertos que toleram essas adversidades bióticas. Nesta pesquisa, o fungo Fusarium oxysporum foi isolado de mudas de viveiro de raízes com sintomas de necrose. Além disso, um isolado deste fungo patogênico modificado com uma cepa de Agrobacterium tumefaciens foi usado para determinar a infecção de F. oxysporum nas raízes dos cultivares Fuerte e Criollo. Os resultados permitem inferir que o cultivar Crioulo apresentou maior tolerância ao F. oxysporum, o que corrobora seu uso como porta-enxerto para variedades comerciais de abacate. Além disso, até onde sabemos, este é o primeiro relato de F. oxysporum afetando mudas de abacate.Research, Society and Development2021-02-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1255410.33448/rsd-v10i2.12554Research, Society and Development; Vol. 10 No. 2; e22010212554Research, Society and Development; Vol. 10 Núm. 2; e22010212554Research, Society and Development; v. 10 n. 2; e220102125542525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/12554/11192Copyright (c) 2021 Grace Estefanía Uribe Hurtado ; William Fernando Viera Arroyo; Álvaro Xavier Sampedro Lema; Kang Jin Cho; Alicia Beatriz Villavicencio Pazos; Francisco Javier Flores Florhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessUribe Hurtado , Grace Estefanía Viera Arroyo, William Fernando Sampedro Lema, Álvaro Xavier Cho, Kang JinVillavicencio Pazos, Alicia Beatriz Flores Flor, Francisco Javier 2021-03-02T09:32:39Zoai:ojs.pkp.sfu.ca:article/12554Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:34:06.085737Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars Colonización de Fusarium oxysporum transformado con el gen de la proteína de fluorescencia roja (tdTomato) mediado por Agrobacterium tumefaciens en raíces de dos cultivares de aguacate Colonização de Fusarium oxysporum transformada com o gene da proteína fluorescente vermelha (tdTomato) mediada por Agrobacterium tumefaciens em raízes de dois cultivares de abacate |
title |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars |
spellingShingle |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars Uribe Hurtado , Grace Estefanía Biotecnología agrícola Criollo Fuerte Higromicina B Microscopia de fluorescencia Porta-enxerto. Agricultural biothecnology Criollo Fuerte Fluorescense microscopy Hygromycin B Rootstock. Biotecnología agrícola Criollo Fuerte Higromicina B Microscopía de fluorescencia Portainjerto. |
title_short |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars |
title_full |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars |
title_fullStr |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars |
title_full_unstemmed |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars |
title_sort |
Colonization of Fusarium oxysporum transformed with the red fluorescence protein gene (tdTomato) mediated by Agrobacterium tumefaciens in roots of two avocado cultivars |
author |
Uribe Hurtado , Grace Estefanía |
author_facet |
Uribe Hurtado , Grace Estefanía Viera Arroyo, William Fernando Sampedro Lema, Álvaro Xavier Cho, Kang Jin Villavicencio Pazos, Alicia Beatriz Flores Flor, Francisco Javier |
author_role |
author |
author2 |
Viera Arroyo, William Fernando Sampedro Lema, Álvaro Xavier Cho, Kang Jin Villavicencio Pazos, Alicia Beatriz Flores Flor, Francisco Javier |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Uribe Hurtado , Grace Estefanía Viera Arroyo, William Fernando Sampedro Lema, Álvaro Xavier Cho, Kang Jin Villavicencio Pazos, Alicia Beatriz Flores Flor, Francisco Javier |
dc.subject.por.fl_str_mv |
Biotecnología agrícola Criollo Fuerte Higromicina B Microscopia de fluorescencia Porta-enxerto. Agricultural biothecnology Criollo Fuerte Fluorescense microscopy Hygromycin B Rootstock. Biotecnología agrícola Criollo Fuerte Higromicina B Microscopía de fluorescencia Portainjerto. |
topic |
Biotecnología agrícola Criollo Fuerte Higromicina B Microscopia de fluorescencia Porta-enxerto. Agricultural biothecnology Criollo Fuerte Fluorescense microscopy Hygromycin B Rootstock. Biotecnología agrícola Criollo Fuerte Higromicina B Microscopía de fluorescencia Portainjerto. |
description |
Avocado (Persea americana) is a fruit crop of economic importance in Ecuador. Currently, a low incidence of Phytophthora cinnamomi has been reported, however, there are other soil pathogens that can affect this crop, even at the initial stages of plant multiplication (nursery), for this reason the use of rootstocks that tolerate these biotic adversities is recommended. In this research, the fungus Fusarium oxysporum was isolated from roots of nursery seedlings with symptoms of necrosis. In addition, an isolate of this pathogenic fungus modified with a strain of Agrobacterium tumefaciens was used to determine the infection of F. oxysporum in the roots of the Fuerte (commercial) and Criollo (local) cultivars. The results allowed to infer that the cultivar Criollo presented a greater tolerance to F. oxysporum than the cultivar Fuerte, which corroborates its use as a rootstock for commercial avocado varieties. Furthermore, to our knowledge, this is the first report of F. oxysporum affecting avocado nursery seedlings in Ecuador. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12554 10.33448/rsd-v10i2.12554 |
url |
https://rsdjournal.org/index.php/rsd/article/view/12554 |
identifier_str_mv |
10.33448/rsd-v10i2.12554 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/12554/11192 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 2; e22010212554 Research, Society and Development; Vol. 10 Núm. 2; e22010212554 Research, Society and Development; v. 10 n. 2; e22010212554 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052745649225728 |