Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets

Detalhes bibliográficos
Autor(a) principal: Oliveira Júnior, João Bosco de
Data de Publicação: 2020
Outros Autores: Pessoa, Camilla Mendes Pedroza, Lopes, Hendril da Silva, Costa, Frederico Henrique da Silva, Pereira, Jonny Everson Scherwinski
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/7031
Resumo: Dragon's blood, native species from South America, has therapeutic properties scientifically proved. This study had the objective of developing a protocol for in vitro establishment and plantlets acclimatization from zygotic embryos, since researches with species are incipient. Culture media (MS, WPM, QL and N6) were assessed, without and with activated charcoal (2 g L-1). It was observed the percentage of developed embryos from 83% to 100% and the effect of medium composition for plant height and leaf number in vitro. The plantlets were acclimatized with 100% of survival rate, and for treatments from in vitro culture only the culture medium influenced diameter and plant height. The WPM and QL formulations without activated charcoal are indicated for in vitro culture of embryos and acclimatization of Dragon blood. The suggested methodology represents an important strategy for reproduction, physiological study and preservation of species.
id UNIFEI_81c54e5be5aabb1d444af6e9c24de0fd
oai_identifier_str oai:ojs.pkp.sfu.ca:article/7031
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantletsProtocolo para el desarrollo de embriones cigóticos in vitro y aclimatación de plántulas de Sangre de dragónProtocolo para desenvolvimento de embriões zigóticos in vitro e aclimatização de plantas de Sangue-de-dragãoPlanta medicinalMeio de culturaCarvão ativadoCroton lechleri.Planta medicinalMedio de cultivoCarbón activadoCroton lechleri.Medicinal plantMedium cultureActive charcoalCroton lechleri.Dragon's blood, native species from South America, has therapeutic properties scientifically proved. This study had the objective of developing a protocol for in vitro establishment and plantlets acclimatization from zygotic embryos, since researches with species are incipient. Culture media (MS, WPM, QL and N6) were assessed, without and with activated charcoal (2 g L-1). It was observed the percentage of developed embryos from 83% to 100% and the effect of medium composition for plant height and leaf number in vitro. The plantlets were acclimatized with 100% of survival rate, and for treatments from in vitro culture only the culture medium influenced diameter and plant height. The WPM and QL formulations without activated charcoal are indicated for in vitro culture of embryos and acclimatization of Dragon blood. The suggested methodology represents an important strategy for reproduction, physiological study and preservation of species.La sangre de dragón, especie nativa de América del Sur, tiene propiedades terapéuticas científicamente comprobadas. Este estudio tuvo el objetivo de desarrollar un protocolo para el establecimiento in vitro y la aclimatación de plántulas a partir de embriones cigóticos, ya que las investigaciones con especies son incipientes. Se evaluaron los medios de cultivo (MS, WPM, QL y N6), sin y con carbón activado (2 g L-1). Se observó el porcentaje de embriones desarrollados del 83% al 100% y el efecto de la composición del medio para la altura de la planta y el número de hojas in vitro. Las plántulas se aclimataron con una tasa de supervivencia del 100%, y para los tratamientos de cultivo in vitro solamente el medio de cultivo influyó en el diámetro y la altura de la planta. Las formulaciones de WPM y QL sin carbón activado están indicadas para el cultivo in vitro de embriones y la aclimatación de sangre de dragón. La metodología sugerida representa una estrategia importante para la reproducción, el estudio fisiológico y la preservación de especies.Sangue-de-dragão, espécie nativa da América do Sul, possui propriedades terapêuticas comprovadas cientificamente. Este estudo teve como objetivo desenvolver um protocolo para estabelecimento in vitro e aclimatização de plantas a partir de embriões zigóticos, uma vez que pesquisas com a espécie são incipientes. Foram avaliados meios de cultura (MS, WPM, QL e N6), na ausência e presença de carvão ativado (2 g L-1). Observou-se percentagem de embriões desenvolvidos entre 83% a 100% e influência da composição do meio para altura de plantas e número de folhas in vitro. As plantas foram aclimatizadas com 100% de sobrevivência, e para os tratamentos no cultivo in vitro apenas o meio de cultura influenciou o diâmetro e altura das plantas. As formulações WPM e QL sem carvão ativado são indicadas para o cultivo in vitro de embriões e aclimatização de Sangue-de-dragão. A metodologia proposta representa uma estratégia para reprodução, estudos fisiológicos e preservação da espécie.Research, Society and Development2020-08-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/703110.33448/rsd-v9i9.7031Research, Society and Development; Vol. 9 No. 9; e231997031Research, Society and Development; Vol. 9 Núm. 9; e231997031Research, Society and Development; v. 9 n. 9; e2319970312525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/7031/6370Copyright (c) 2020 João Bosco Oliveira Júniorhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira Júnior, João Bosco de Pessoa, Camilla Mendes Pedroza Lopes, Hendril da SilvaCosta, Frederico Henrique da Silva Pereira, Jonny Everson Scherwinski 2020-09-18T01:42:11Zoai:ojs.pkp.sfu.ca:article/7031Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:29:54.560524Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets
Protocolo para el desarrollo de embriones cigóticos in vitro y aclimatación de plántulas de Sangre de dragón
Protocolo para desenvolvimento de embriões zigóticos in vitro e aclimatização de plantas de Sangue-de-dragão
title Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets
spellingShingle Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets
Oliveira Júnior, João Bosco de
Planta medicinal
Meio de cultura
Carvão ativado
Croton lechleri.
Planta medicinal
Medio de cultivo
Carbón activado
Croton lechleri.
Medicinal plant
Medium culture
Active charcoal
Croton lechleri.
title_short Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets
title_full Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets
title_fullStr Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets
title_full_unstemmed Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets
title_sort Protocol for development of zygotic embryos in vitro and acclimatization of Dragon’s Blood plantlets
author Oliveira Júnior, João Bosco de
author_facet Oliveira Júnior, João Bosco de
Pessoa, Camilla Mendes Pedroza
Lopes, Hendril da Silva
Costa, Frederico Henrique da Silva
Pereira, Jonny Everson Scherwinski
author_role author
author2 Pessoa, Camilla Mendes Pedroza
Lopes, Hendril da Silva
Costa, Frederico Henrique da Silva
Pereira, Jonny Everson Scherwinski
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Oliveira Júnior, João Bosco de
Pessoa, Camilla Mendes Pedroza
Lopes, Hendril da Silva
Costa, Frederico Henrique da Silva
Pereira, Jonny Everson Scherwinski
dc.subject.por.fl_str_mv Planta medicinal
Meio de cultura
Carvão ativado
Croton lechleri.
Planta medicinal
Medio de cultivo
Carbón activado
Croton lechleri.
Medicinal plant
Medium culture
Active charcoal
Croton lechleri.
topic Planta medicinal
Meio de cultura
Carvão ativado
Croton lechleri.
Planta medicinal
Medio de cultivo
Carbón activado
Croton lechleri.
Medicinal plant
Medium culture
Active charcoal
Croton lechleri.
description Dragon's blood, native species from South America, has therapeutic properties scientifically proved. This study had the objective of developing a protocol for in vitro establishment and plantlets acclimatization from zygotic embryos, since researches with species are incipient. Culture media (MS, WPM, QL and N6) were assessed, without and with activated charcoal (2 g L-1). It was observed the percentage of developed embryos from 83% to 100% and the effect of medium composition for plant height and leaf number in vitro. The plantlets were acclimatized with 100% of survival rate, and for treatments from in vitro culture only the culture medium influenced diameter and plant height. The WPM and QL formulations without activated charcoal are indicated for in vitro culture of embryos and acclimatization of Dragon blood. The suggested methodology represents an important strategy for reproduction, physiological study and preservation of species.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/7031
10.33448/rsd-v9i9.7031
url https://rsdjournal.org/index.php/rsd/article/view/7031
identifier_str_mv 10.33448/rsd-v9i9.7031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/7031/6370
dc.rights.driver.fl_str_mv Copyright (c) 2020 João Bosco Oliveira Júnior
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 João Bosco Oliveira Júnior
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 9; e231997031
Research, Society and Development; Vol. 9 Núm. 9; e231997031
Research, Society and Development; v. 9 n. 9; e231997031
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052656017997824

Registros relacionados