Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect

Detalhes bibliográficos
Autor(a) principal: Vale, Giovanna Caroline Aparecida do
Data de Publicação: 2021
Outros Autores: Fernandes, Kelly Rossetti, Parisi, Julia Risso, Santana, Alan de França, Cruz, Matheus de Almeida, Fortulan, Carlos Alberto, Zanotto, Edgar Dutra, Peitl, Oscar, Granito, Renata Neves, Rennó, Ana Claudia Muniz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/16498
Resumo: The study comparing the bone regenerative capacity in an experimental model of cranial bone defects in rats, into 3 groups: G1: bone defects irradiated with photobiomodulation; G2: Biosilicate + photobiomodulation and G3: Biosilicate and Spongin + photobiomodulation. Histocompatibility and bone responses were performed after 15 and 45 days of implantation. Histological analysis demonstrated that photobiomodulation irradiated animals presented an increased amount of newly formed over time. Histomorphometry showed higher values for bone volume for G3 and G1, higher values for osteoid volume and number of osteoblasts observed for G3 compared to G2. TGF-β immunolabelling was higher for G2. The values found for VEGF were higher for biosilicate (with or without spongin) 15 days of implantation with an increased difference being observed for G1, 45 days after surgery. In conclusion, the stimulus provided by photobiomodulation associated to the biomimetic composite increased bone formation in the cranial bone defect in rats. Consequently, these data highlight the potential of the introduction of spongin into biosilicate and irradiated with photobiomodulation to improve the biological performance for bone regeneration applications.
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spelling Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect Asociación de compuestos de colágeno marino/biosilicato y fotobiomodulación en el proceso de cicatrización ósea utilizando un modelo experimental de defecto de calvariaAssociação de compósitos de colágeno marinho/biosilicato e fotobiomodulação no processo de consolidação óssea usando um modelo experimental de defeito na calváriaFotobiomodulaçãoBiomateriaisEngenharia tecidual.FotobiomodulaciónBiomaterialesIngeniería de tejidos.PhotobiomodulationBiomaterialsTissue engineering.The study comparing the bone regenerative capacity in an experimental model of cranial bone defects in rats, into 3 groups: G1: bone defects irradiated with photobiomodulation; G2: Biosilicate + photobiomodulation and G3: Biosilicate and Spongin + photobiomodulation. Histocompatibility and bone responses were performed after 15 and 45 days of implantation. Histological analysis demonstrated that photobiomodulation irradiated animals presented an increased amount of newly formed over time. Histomorphometry showed higher values for bone volume for G3 and G1, higher values for osteoid volume and number of osteoblasts observed for G3 compared to G2. TGF-β immunolabelling was higher for G2. The values found for VEGF were higher for biosilicate (with or without spongin) 15 days of implantation with an increased difference being observed for G1, 45 days after surgery. In conclusion, the stimulus provided by photobiomodulation associated to the biomimetic composite increased bone formation in the cranial bone defect in rats. Consequently, these data highlight the potential of the introduction of spongin into biosilicate and irradiated with photobiomodulation to improve the biological performance for bone regeneration applications.El estudio comparó la capacidad de regeneración ósea en un modelo experimental de defectos óseos craneales en ratas, en 3 grupos: G1: defectos óseos irradiados con fotobiomodulación; G2: Biosilicato + fotobiomodulación y G3: Biosilicato y Spongin + fotobiomodulación. La histocompatibilidad y las respuestas óseas se realizaron a los 15 y 45 días de la implantación. El análisis histológico demostró que los animales irradiados con fotobiomodulación presentaron una mayor cantidad de recién formados con el tiempo. La histomorfometría mostró valores más altos para el volumen óseo para G3 y G1, valores más altos para el volumen osteoide y el número de osteoblastos observados para G3 en comparación con G2. El inmunomarcaje de TGF-β fue mayor para G2. Los valores encontrados para VEGF fueron mayores para el biosilicato (con o sin esponja) a los 15 días de la implantación, observándose una mayor diferencia para G1, 45 días después de la cirugía. En conclusión, el estímulo proporcionado por la fotobiomodulación asociada al compuesto biomimético aumentó la formación de hueso en el defecto óseo craneal en ratas. En consecuencia, estos datos destacan el potencial de la introducción de espongina en biosilicato e irradiado con fotobiomodulación para mejorar el rendimiento biológico para aplicaciones de regeneración ósea.O estudo comparou a capacidade regenerativa óssea em modelo experimental de defeitos ósseos cranianos em ratos, em 3 grupos: G1: defeitos ósseos irradiados com fotobiomodulação; G2: Biosilicato + fotobiomodulação e G3: Biosilicato e esponja marinha + fotobiomodulação. A histocompatibilidade e as respostas ósseas foram realizadas após 15 e 45 dias de implantação. A análise histológica demonstrou que os animais irradiados com fotobiomodulação apresentaram um aumento da quantidade de neoformados ao longo do tempo. A histomorfometria mostrou maiores valores de volume ósseo para G3 e G1, maiores valores de volume de osteóide e número de osteoblastos observados para G3 em relação ao G2. A imunomarcação de TGF-β foi maior para G2. Os valores encontrados para VEGF foram maiores para o biosilicato (com ou sem esponja marinha) 15 dias após o implante, com diferença aumentada sendo observada para o G1, 45 dias após a cirurgia. Em conclusão, o estímulo fornecido pela fotobiomodulação associada ao compósito biomimético aumentou a formação óssea no defeito ósseo craniano em ratos. Consequentemente, esses dados destacam o potencial da introdução da esponja marinha no biosilicato e irradiada com fotobiomodulação para melhorar o desempenho biológico para aplicações de regeneração óssea.Research, Society and Development2021-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1649810.33448/rsd-v10i8.16498Research, Society and Development; Vol. 10 No. 8; e8610816498Research, Society and Development; Vol. 10 Núm. 8; e8610816498Research, Society and Development; v. 10 n. 8; e86108164982525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/16498/15231Copyright (c) 2021 Giovanna Caroline Aparecida do Vale; Kelly Rossetti Fernandes; Julia Risso Parisi; Alan de França Santana; Matheus de Almeida Cruz; Carlos Alberto Fortulan; Edgar Dutra Zanotto; Oscar Peitl; Renata Neves Granito; Ana Claudia Muniz Rennóhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVale, Giovanna Caroline Aparecida doFernandes, Kelly Rossetti Parisi, Julia RissoSantana, Alan de França Cruz, Matheus de AlmeidaFortulan, Carlos AlbertoZanotto, Edgar DutraPeitl, OscarGranito, Renata NevesRennó, Ana Claudia Muniz2021-08-21T18:46:59Zoai:ojs.pkp.sfu.ca:article/16498Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:01.775003Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect
Asociación de compuestos de colágeno marino/biosilicato y fotobiomodulación en el proceso de cicatrización ósea utilizando un modelo experimental de defecto de calvaria
Associação de compósitos de colágeno marinho/biosilicato e fotobiomodulação no processo de consolidação óssea usando um modelo experimental de defeito na calvária
title Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect
spellingShingle Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect
Vale, Giovanna Caroline Aparecida do
Fotobiomodulação
Biomateriais
Engenharia tecidual.
Fotobiomodulación
Biomateriales
Ingeniería de tejidos.
Photobiomodulation
Biomaterials
Tissue engineering.
title_short Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect
title_full Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect
title_fullStr Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect
title_full_unstemmed Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect
title_sort Association of marine Collagen/Biosilicate composites and photobiomodulation in the process of bone healing using an experimental model of calvarial defect
author Vale, Giovanna Caroline Aparecida do
author_facet Vale, Giovanna Caroline Aparecida do
Fernandes, Kelly Rossetti
Parisi, Julia Risso
Santana, Alan de França
Cruz, Matheus de Almeida
Fortulan, Carlos Alberto
Zanotto, Edgar Dutra
Peitl, Oscar
Granito, Renata Neves
Rennó, Ana Claudia Muniz
author_role author
author2 Fernandes, Kelly Rossetti
Parisi, Julia Risso
Santana, Alan de França
Cruz, Matheus de Almeida
Fortulan, Carlos Alberto
Zanotto, Edgar Dutra
Peitl, Oscar
Granito, Renata Neves
Rennó, Ana Claudia Muniz
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vale, Giovanna Caroline Aparecida do
Fernandes, Kelly Rossetti
Parisi, Julia Risso
Santana, Alan de França
Cruz, Matheus de Almeida
Fortulan, Carlos Alberto
Zanotto, Edgar Dutra
Peitl, Oscar
Granito, Renata Neves
Rennó, Ana Claudia Muniz
dc.subject.por.fl_str_mv Fotobiomodulação
Biomateriais
Engenharia tecidual.
Fotobiomodulación
Biomateriales
Ingeniería de tejidos.
Photobiomodulation
Biomaterials
Tissue engineering.
topic Fotobiomodulação
Biomateriais
Engenharia tecidual.
Fotobiomodulación
Biomateriales
Ingeniería de tejidos.
Photobiomodulation
Biomaterials
Tissue engineering.
description The study comparing the bone regenerative capacity in an experimental model of cranial bone defects in rats, into 3 groups: G1: bone defects irradiated with photobiomodulation; G2: Biosilicate + photobiomodulation and G3: Biosilicate and Spongin + photobiomodulation. Histocompatibility and bone responses were performed after 15 and 45 days of implantation. Histological analysis demonstrated that photobiomodulation irradiated animals presented an increased amount of newly formed over time. Histomorphometry showed higher values for bone volume for G3 and G1, higher values for osteoid volume and number of osteoblasts observed for G3 compared to G2. TGF-β immunolabelling was higher for G2. The values found for VEGF were higher for biosilicate (with or without spongin) 15 days of implantation with an increased difference being observed for G1, 45 days after surgery. In conclusion, the stimulus provided by photobiomodulation associated to the biomimetic composite increased bone formation in the cranial bone defect in rats. Consequently, these data highlight the potential of the introduction of spongin into biosilicate and irradiated with photobiomodulation to improve the biological performance for bone regeneration applications.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16498
10.33448/rsd-v10i8.16498
url https://rsdjournal.org/index.php/rsd/article/view/16498
identifier_str_mv 10.33448/rsd-v10i8.16498
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16498/15231
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 8; e8610816498
Research, Society and Development; Vol. 10 Núm. 8; e8610816498
Research, Society and Development; v. 10 n. 8; e8610816498
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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