Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/6975 |
Resumo: | Pseudomonas aeruginosa is one of the most frequent pathogens in infections affecting burn patients, having as an important determinant of pathogenicity its ability to form biofilms. The present work aims to evaluate the effectiveness of biocidal solutions: 4% chlorhexidine, 1% sodium hypochlorite, 5% hydrogen peroxide and 70% alcohol on removal P. aeruginosa multidrug-resistant biofilms. This is a descriptive study with a quantitative approach. Biofilms from nine strains of P. aeruginosa, of different clonal types, were evaluated. Eight strains were carbapenem-resistant and one susceptible, collected from burned patients and balneotherapy tables. Strains were grown together with stainless steel coupons and incubated for 24 h. Subsequently, the coupons were treated with and without biocides, at contact times of 15, 5 and 0 minutes. Biofilm removal was assessed by counting viable cells obtained from coupons with and without treatment. The minimum inhibitory concentration for imipenem and meropenem was determined by using the epsilometric method. Biofilm formation was observed in all coupons not treated with biocides. However, there was no count of viable cells after contact of the biofilm with all biocidal solutions, in all times. The minimum inhibitory concentration to carbapenems varied between 16 to ≥ 32µg / mL. It was concluded that all the disinfectant solutions evaluated were effective in removing biofilms produced by the studied P. aeruginosa strains. |
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Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosaEvaluación de la eficacia de los biocidas en la eliminación de biopelículas producidas por Pseudomonas aeruginosa resistente a múltiples fármacosAvaliação da eficácia de biocidas na remoção de biofilmes produzidos por Pseudomonas aeruginosa multidroga resistentesPseudomonas aeruginosaBiopelículasBiocidasDesinfecciónCarbapenémicos.Pseudomonas aeruginosaBiofilmsBiocidesCarbapenems.Pseudomonas aeruginosaBiofilmesBiocidasDesinfecçãoCarbapenêmicos.Pseudomonas aeruginosa is one of the most frequent pathogens in infections affecting burn patients, having as an important determinant of pathogenicity its ability to form biofilms. The present work aims to evaluate the effectiveness of biocidal solutions: 4% chlorhexidine, 1% sodium hypochlorite, 5% hydrogen peroxide and 70% alcohol on removal P. aeruginosa multidrug-resistant biofilms. This is a descriptive study with a quantitative approach. Biofilms from nine strains of P. aeruginosa, of different clonal types, were evaluated. Eight strains were carbapenem-resistant and one susceptible, collected from burned patients and balneotherapy tables. Strains were grown together with stainless steel coupons and incubated for 24 h. Subsequently, the coupons were treated with and without biocides, at contact times of 15, 5 and 0 minutes. Biofilm removal was assessed by counting viable cells obtained from coupons with and without treatment. The minimum inhibitory concentration for imipenem and meropenem was determined by using the epsilometric method. Biofilm formation was observed in all coupons not treated with biocides. However, there was no count of viable cells after contact of the biofilm with all biocidal solutions, in all times. The minimum inhibitory concentration to carbapenems varied between 16 to ≥ 32µg / mL. It was concluded that all the disinfectant solutions evaluated were effective in removing biofilms produced by the studied P. aeruginosa strains.Pseudomonas aeruginosa es uno de los patógenos más frecuentes en infecciones que afectan a pacientes con quemaduras, teniendo como determinante importante de patogenicidad su capacidad para formar biopelículas. El presente trabajo tiene como objetivo evaluar la efectividad de las soluciones biocidas: clorhexidina al 4%, hipoclorito de sodio al 1%, peróxido de hidrógeno al 5% y alcohol al 70% en la eliminación de biopelículas de P. aeruginosa multidrogas resistentes. Este es un estudio descriptivo con un enfoque cuantitativo. Se evaluaron biopelículas de nueve cepas de P. aeruginosa, de diferentes tipos clonales, ocho resistentes a carbapenems y una sensible, recolectada de pacientes quemados y tablas de balneoterapia. Las cepas estudiadas se cultivaron junto con cupones de acero inoxidable, y se incubaron durante 24 h. Posteriormente, los cupones se trataron con y sin biocidas, en tiempos de contacto de 15, 5 y 0 minutos. La eliminación de la biopelícula se evaluó contando las células viables obtenidas de los cupones con y sin tratamiento. La concentración inhibitoria mínima para imipenem y meropenem se determinó para cepas evaluadas usando el método epsilométrico. Se observó la formación de biopelículas en todos los cupones no tratados con biocidas. Sin embargo, no hubo recuento de células viables después del contacto de la biopelícula con todas las soluciones biocidas, en los tiempos estudiados. La concentración inhibitoria mínima para los carbapenémicos varió entre 16 y ≥ 32 µg / ml. Se concluyó que todas las soluciones desinfectantes evaluadas fueron efectivas para eliminar las biopelículas producidas por las cepas estudiadas de P. aeruginosa.Pseudomonas aeruginosa é um dos patógenos mais frequentes em infecções acometendo pacientes queimados, tendo como importante determinante de patogenicidade sua capacidade de formar biofilmes. O presente trabalho tem por objetivo avaliar a eficácia das soluções biocidas: clorexidina 4%, hipoclorito de sódio 1%, peróxido de hidrogênio 5% e álcool 70% na remoção de biofilmes de P. aeruginosa multidroga resistentes. Trata-se de um estudo descritivo de abordagem quantitativa. Foram avaliados biofilmes de nove cepas de P. aeruginosa, de diferentes tipos clonais, sendo oito resistentes a carbapenêmicos e uma sensível, coletadas de pacientes queimados e mesas de balneoterapia. As cepas estudas foram cultivadas juntamente com cupons de aço inoxidável, e incubadas por 24 h. Posteriormente, os cupons foram tratados com e sem biocidas, nos tempos de contato de 15, 5 e 0 minutos. A remoção do biofilme foi avaliada através da contagem de células viáveis obtidas dos cupons com e sem tratamento. A concentração mínima inibitória ao imipenem e meropenem foi determinada para cepas avaliadas através do método epsilométrico. Foi observado formação de biofilme em todos os cupons não tratados com biocidas. Entretanto, não houve contagem de células viáveis após contato do biofilme com todas soluções biocidas, nos tempos estudados. A concentração mínima inibitória aos carbapenêmicos variou entre 16 a ≥ 32µg/mL. Concluiu-se que todas a soluções desinfetantes avaliadas, foram eficazes na remoção de biofilmes produzidos pelas cepas de P. aeruginosa estudadas.Research, Society and Development2020-08-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/697510.33448/rsd-v9i9.6975Research, Society and Development; Vol. 9 No. 9; e83996975Research, Society and Development; Vol. 9 Núm. 9; e83996975Research, Society and Development; v. 9 n. 9; e839969752525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/6975/6206Copyright (c) 2020 Keila de Cássia Ferreira de Almeida Silva, Mariana Alcântara Calomino, Gabriela Deutsch, Bruna Maiara Ferreira Barreto Pires, Lenise Arneiro Teixeira, Geraldo Renato de Paulahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Keila de Cássia Ferreira de AlmeidaCalomino, Mariana AlcântaraDeutsch, GabrielaPires, Bruna Maiara Ferreira BarretoEsper, Luciana Maria Ramires Paula, Geraldo Renato deTeixeira, Lenise Arneiro2020-09-18T01:42:11Zoai:ojs.pkp.sfu.ca:article/6975Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:29:52.994542Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa Evaluación de la eficacia de los biocidas en la eliminación de biopelículas producidas por Pseudomonas aeruginosa resistente a múltiples fármacos Avaliação da eficácia de biocidas na remoção de biofilmes produzidos por Pseudomonas aeruginosa multidroga resistentes |
| title |
Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa |
| spellingShingle |
Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa Silva, Keila de Cássia Ferreira de Almeida Pseudomonas aeruginosa Biopelículas Biocidas Desinfección Carbapenémicos. Pseudomonas aeruginosa Biofilms Biocides Carbapenems. Pseudomonas aeruginosa Biofilmes Biocidas Desinfecção Carbapenêmicos. |
| title_short |
Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa |
| title_full |
Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa |
| title_fullStr |
Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa |
| title_full_unstemmed |
Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa |
| title_sort |
Evaluation of the efficacy of biocides in removing biofilms produced by multidrug-resistant Pseudomonas aeruginosa |
| author |
Silva, Keila de Cássia Ferreira de Almeida |
| author_facet |
Silva, Keila de Cássia Ferreira de Almeida Calomino, Mariana Alcântara Deutsch, Gabriela Pires, Bruna Maiara Ferreira Barreto Esper, Luciana Maria Ramires Paula, Geraldo Renato de Teixeira, Lenise Arneiro |
| author_role |
author |
| author2 |
Calomino, Mariana Alcântara Deutsch, Gabriela Pires, Bruna Maiara Ferreira Barreto Esper, Luciana Maria Ramires Paula, Geraldo Renato de Teixeira, Lenise Arneiro |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Keila de Cássia Ferreira de Almeida Calomino, Mariana Alcântara Deutsch, Gabriela Pires, Bruna Maiara Ferreira Barreto Esper, Luciana Maria Ramires Paula, Geraldo Renato de Teixeira, Lenise Arneiro |
| dc.subject.por.fl_str_mv |
Pseudomonas aeruginosa Biopelículas Biocidas Desinfección Carbapenémicos. Pseudomonas aeruginosa Biofilms Biocides Carbapenems. Pseudomonas aeruginosa Biofilmes Biocidas Desinfecção Carbapenêmicos. |
| topic |
Pseudomonas aeruginosa Biopelículas Biocidas Desinfección Carbapenémicos. Pseudomonas aeruginosa Biofilms Biocides Carbapenems. Pseudomonas aeruginosa Biofilmes Biocidas Desinfecção Carbapenêmicos. |
| description |
Pseudomonas aeruginosa is one of the most frequent pathogens in infections affecting burn patients, having as an important determinant of pathogenicity its ability to form biofilms. The present work aims to evaluate the effectiveness of biocidal solutions: 4% chlorhexidine, 1% sodium hypochlorite, 5% hydrogen peroxide and 70% alcohol on removal P. aeruginosa multidrug-resistant biofilms. This is a descriptive study with a quantitative approach. Biofilms from nine strains of P. aeruginosa, of different clonal types, were evaluated. Eight strains were carbapenem-resistant and one susceptible, collected from burned patients and balneotherapy tables. Strains were grown together with stainless steel coupons and incubated for 24 h. Subsequently, the coupons were treated with and without biocides, at contact times of 15, 5 and 0 minutes. Biofilm removal was assessed by counting viable cells obtained from coupons with and without treatment. The minimum inhibitory concentration for imipenem and meropenem was determined by using the epsilometric method. Biofilm formation was observed in all coupons not treated with biocides. However, there was no count of viable cells after contact of the biofilm with all biocidal solutions, in all times. The minimum inhibitory concentration to carbapenems varied between 16 to ≥ 32µg / mL. It was concluded that all the disinfectant solutions evaluated were effective in removing biofilms produced by the studied P. aeruginosa strains. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-08-13 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/6975 10.33448/rsd-v9i9.6975 |
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https://rsdjournal.org/index.php/rsd/article/view/6975 |
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10.33448/rsd-v9i9.6975 |
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por |
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por |
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https://rsdjournal.org/index.php/rsd/article/view/6975/6206 |
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http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 9 No. 9; e83996975 Research, Society and Development; Vol. 9 Núm. 9; e83996975 Research, Society and Development; v. 9 n. 9; e83996975 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052655982346240 |