Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)

Detalhes bibliográficos
Autor(a) principal: Corpes, Rosana Silva
Data de Publicação: 2021
Outros Autores: Santos, Alberdan Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/20378
Resumo: Crinum americanum L. is a monocotyledonous plant belonging to the Amaryllidaceae family that has economic importance for being ornamental and pharmacological for containg alkaloids that can present anticholinesterase action in its chemical composition. Callus induction is important both for biomass production and for the accumulation of secondary metabolites, especially those of the alkaloid class. In this sense, biotechnological techniques can be used as an alternative to make the process viable. Nowadays, it is known that monocotyledonous plants present great difficulties to be cultivated in vitro, among which the species C. americanum stands out. As a result, the objective this study was to demonstrate the most efficient concentrations of growth regulators 2,4-D and BAP associated with solidifying agents Agar and Phytagel for in vitro cultivation of this species in order to present alternatives to increase production of biomass and help in future studies in the area of ​​somatic embryogenesis and/or for accumulation of secondary metabolites. For the evaluation of cell development, growth in diameter was observed and the callus growth curve was also obtained through sacrificial sampling. It was observed that when the friable callus is inoculated with the correct balance between growth regulators in MS medium supplemented with 3% sucrose (m/v), which contains the solidifying agent Phytagel at 0.2% (m/v), cells tend to grow faster and consequently the gain in biomass is greater.
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spelling Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)Influencia de los reguladores de crecimiento 2,4-D y BAP asociados con agentes solidificantes Agar y Phytagel en la inducción de callos de Crinum americanum L. (Amaryllidaceae)Influência dos Reguladores de Crescimento 2,4-D e BAP associados aos agentes solidificantes Ágar e Phytagel na Indução de calos de Crinum americanum L. (Amaryllidaceae)AlcalóidesBiotecnologia vegetalCultivo in vitro.AlkaloidsPlant biotechnologyIn vitro cultivation.AlcalóidesBiotecnologia vegetalCultivo in vitro.Crinum americanum L. is a monocotyledonous plant belonging to the Amaryllidaceae family that has economic importance for being ornamental and pharmacological for containg alkaloids that can present anticholinesterase action in its chemical composition. Callus induction is important both for biomass production and for the accumulation of secondary metabolites, especially those of the alkaloid class. In this sense, biotechnological techniques can be used as an alternative to make the process viable. Nowadays, it is known that monocotyledonous plants present great difficulties to be cultivated in vitro, among which the species C. americanum stands out. As a result, the objective this study was to demonstrate the most efficient concentrations of growth regulators 2,4-D and BAP associated with solidifying agents Agar and Phytagel for in vitro cultivation of this species in order to present alternatives to increase production of biomass and help in future studies in the area of ​​somatic embryogenesis and/or for accumulation of secondary metabolites. For the evaluation of cell development, growth in diameter was observed and the callus growth curve was also obtained through sacrificial sampling. It was observed that when the friable callus is inoculated with the correct balance between growth regulators in MS medium supplemented with 3% sucrose (m/v), which contains the solidifying agent Phytagel at 0.2% (m/v), cells tend to grow faster and consequently the gain in biomass is greater.Crinum americanum L. es una planta monocotiledónea perteneciente a la familia de las amaryllidaceae que tiene importancia económica ser ornamental e farmacologica por contener alcaloides que pueden presentar acción anticolinesterasa en su composición química. la inducción de callos es importante tanto para la producción de biomasa como el acumulo de metabolitos secundarios, especialmente los de la clase de alcaloides. En este sentido, las técnicas biotecnológicas se pueden utilizar como alternativa para viabilizar el proceso. Actualmente, se sabe que las plantas monocotiledóneas presentan grandes dificultades para ser cultivadas in vitro, entre las que destaca la especie C. americanum. Como resultado, este estudio tuvo como objetivo demostrar las concentraciones más eficientes de reguladores de crecimiento 2,4-D y BAP asociados con agentes solidificantes Agar y Phytagel para el cultivo in vitro de esta especie con el fin de presentar alternativas para incrementar la producción de biomasa y ayudar en estudios futuros en el área de embriogénesis somática o acumulación de metabolitos secundarios. Para la evaluación del desarrollo celular, se observó crecimiento en diámetro y también se obtuvo la curva de crecimiento del callo mediante muestreo de sacrificio. Se observó que cuando se inoculan callos friables con el equilibrio correcto entre reguladores de crecimiento en medio MS suplementado con sacarosa al 3% (m / v), que contiene el agente solidificante Phytagel al 0.2% (m / v), las células tienden a crecer más rápido y consecuentemente se gano mas biomasa.Crinum americanum L. é uma planta monocotiledônea pertencente à família Amaryllidaceae que possui importância econômica por ser ornamental e farmacológica por conter alcalóides que podem apresentar ação anticolinisterásica em sua composição química. A indução de calos é importante tanto para produção de biomassa como para o acúmulo de metabólitos secundários, principalmente aos da classe dos alcaloides. Neste sentido, podem ser empregadas técnicas biotecnológicas como alternativa para viabilizar o processo. Atualmente, sabe-se que as plantas monocotiledôneas apresentam grandes dificuldades de serem cultivadas in vitro, dentre as quais se destaca a espécie C. americanum. Em função disso, este estudo teve o objetivo de demostrar as concentrações mais eficientes dos reguladores de crescimento 2,4-D e BAP associados aos agentes solidificantes Ágar e Phytagel para cultivo in vitro desta espécie com o intuito de apresentar alternativas para o aumento da produção de biomassa e auxiliar em estudos futuros na área de embriogênese somática e/ou para acúmulo de metabólitos secundários. Para a avaliação do desenvolvimento das células, observou-se o crescimento das mesmas em diâmetro e também se obteve a curva de crescimento dos calos através da amostragem por sacrifício. Observou-se que quando os calos friáveis são inoculados com o correto balanço entre os reguladores de crescimento no meio MS suplementado com sacarose 3% (m/v), que contenha o agente solidificante Phytagel a 0,2 % (m/v), as células tendem a crescer com maior velocidade e consequentemente o ganho de biomassa é maior.Research, Society and Development2021-09-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2037810.33448/rsd-v10i12.20378Research, Society and Development; Vol. 10 No. 12; e299101220378Research, Society and Development; Vol. 10 Núm. 12; e299101220378Research, Society and Development; v. 10 n. 12; e2991012203782525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/20378/18266Copyright (c) 2021 Rosana Silva Corpes; Alberdan Silva Santoshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCorpes, Rosana Silva Santos, Alberdan Silva2021-11-14T20:26:51Zoai:ojs.pkp.sfu.ca:article/20378Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:40:02.747047Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
Influencia de los reguladores de crecimiento 2,4-D y BAP asociados con agentes solidificantes Agar y Phytagel en la inducción de callos de Crinum americanum L. (Amaryllidaceae)
Influência dos Reguladores de Crescimento 2,4-D e BAP associados aos agentes solidificantes Ágar e Phytagel na Indução de calos de Crinum americanum L. (Amaryllidaceae)
title Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
spellingShingle Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
Corpes, Rosana Silva
Alcalóides
Biotecnologia vegetal
Cultivo in vitro.
Alkaloids
Plant biotechnology
In vitro cultivation.
Alcalóides
Biotecnologia vegetal
Cultivo in vitro.
title_short Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
title_full Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
title_fullStr Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
title_full_unstemmed Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
title_sort Influence of 2,4-D and BAP Growth Regulators associated with Agar solidifying agents and Phytagel on callus induction of Crinum americanum L. (Amaryllidaceae)
author Corpes, Rosana Silva
author_facet Corpes, Rosana Silva
Santos, Alberdan Silva
author_role author
author2 Santos, Alberdan Silva
author2_role author
dc.contributor.author.fl_str_mv Corpes, Rosana Silva
Santos, Alberdan Silva
dc.subject.por.fl_str_mv Alcalóides
Biotecnologia vegetal
Cultivo in vitro.
Alkaloids
Plant biotechnology
In vitro cultivation.
Alcalóides
Biotecnologia vegetal
Cultivo in vitro.
topic Alcalóides
Biotecnologia vegetal
Cultivo in vitro.
Alkaloids
Plant biotechnology
In vitro cultivation.
Alcalóides
Biotecnologia vegetal
Cultivo in vitro.
description Crinum americanum L. is a monocotyledonous plant belonging to the Amaryllidaceae family that has economic importance for being ornamental and pharmacological for containg alkaloids that can present anticholinesterase action in its chemical composition. Callus induction is important both for biomass production and for the accumulation of secondary metabolites, especially those of the alkaloid class. In this sense, biotechnological techniques can be used as an alternative to make the process viable. Nowadays, it is known that monocotyledonous plants present great difficulties to be cultivated in vitro, among which the species C. americanum stands out. As a result, the objective this study was to demonstrate the most efficient concentrations of growth regulators 2,4-D and BAP associated with solidifying agents Agar and Phytagel for in vitro cultivation of this species in order to present alternatives to increase production of biomass and help in future studies in the area of ​​somatic embryogenesis and/or for accumulation of secondary metabolites. For the evaluation of cell development, growth in diameter was observed and the callus growth curve was also obtained through sacrificial sampling. It was observed that when the friable callus is inoculated with the correct balance between growth regulators in MS medium supplemented with 3% sucrose (m/v), which contains the solidifying agent Phytagel at 0.2% (m/v), cells tend to grow faster and consequently the gain in biomass is greater.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/20378
10.33448/rsd-v10i12.20378
url https://rsdjournal.org/index.php/rsd/article/view/20378
identifier_str_mv 10.33448/rsd-v10i12.20378
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/20378/18266
dc.rights.driver.fl_str_mv Copyright (c) 2021 Rosana Silva Corpes; Alberdan Silva Santos
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Rosana Silva Corpes; Alberdan Silva Santos
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 12; e299101220378
Research, Society and Development; Vol. 10 Núm. 12; e299101220378
Research, Society and Development; v. 10 n. 12; e299101220378
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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