Micropropagation on multiplication of native greenberries in South of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/11788 |
Resumo: | Rubus brasiliensis and Rubus erythroclados are commonly known as greenberries. This work aimed to test in vitro micropropagation protocols to greenberries. The multiplication experiment was conducted using BAP at concentrations 1 mg L-1, 2 mg L-1 and 3 mg L-1. The rooting experiment was carried out in ½ MS medium without the addition of hormones, using 0.1 mg L-1 NAA or 1.0 mg L-1 IBA. The highest rate of viability of explants was obtained with R. brasiliensis propagation using 1.0 mg L-1 of BAP. Rooting explants showed survival rates higher than 85%. Rooting rates in R. erythroclados was 87% while in R. brasiliensis it was 38%, The highest rates were obtained using 1.0 mg L-1 IBA in R. erythroclados.The acclimatization reached survival rates of 75% when rooted using NAA and ½ MS and 86% when rooted with IBA. In vitro culture shows to be a viable method to propagate greenberries. |
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Micropropagation on multiplication of native greenberries in South of Brazil Micropropagación en la multiplicación de moras verdes originarias del sur de BrasilMicropropagação na multiplicação de amoreiras-verdes nativas do Sul do Brasil Rubus brasiliensisRubus erythrocladosIn vitro cultivationDomestication.Rubus brasiliensisRubus erythrocladosCultivo in vitroDomesticación.Rubus brasiliensisRubus erythrocladosCultivo in vitroDomesticação.Rubus brasiliensis and Rubus erythroclados are commonly known as greenberries. This work aimed to test in vitro micropropagation protocols to greenberries. The multiplication experiment was conducted using BAP at concentrations 1 mg L-1, 2 mg L-1 and 3 mg L-1. The rooting experiment was carried out in ½ MS medium without the addition of hormones, using 0.1 mg L-1 NAA or 1.0 mg L-1 IBA. The highest rate of viability of explants was obtained with R. brasiliensis propagation using 1.0 mg L-1 of BAP. Rooting explants showed survival rates higher than 85%. Rooting rates in R. erythroclados was 87% while in R. brasiliensis it was 38%, The highest rates were obtained using 1.0 mg L-1 IBA in R. erythroclados.The acclimatization reached survival rates of 75% when rooted using NAA and ½ MS and 86% when rooted with IBA. In vitro culture shows to be a viable method to propagate greenberries.Rubus brasiliensis y Rubus erythroclados se conocen comúnmente como morera verde. Este trabajo tuvo como objetivo probar protocolos de micropropagación in vitro a morera verde. El experimento de multiplicación se realizó utilizando BAP en concentraciones de 1 mg L-1, 2 mg L-1 y 3 mg L-1. El experimento de enraizamiento se llevó a cabo en medio ½ MS sin la adición de hormonas, utilizando 0.1 mg L-1 NAA o 1.0 mg L-1 IBA. La mayor tasa de viabilidad de explantes se obtuvo con la propagación de R. brasiliensis usando 1.0 mg L-1 de BAP. Los explantes de enraizamiento mostraron tasas de supervivencia superiores al 85%. La tasa de enraizamiento en R. erythroclados fue del 87% mientras que en R. brasiliensis fue del 38%, las tasas más altas se obtuvieron con 1.0 mg L-1 de AIB en R. erythroclados, la aclimatación alcanzó tasas de supervivencia del 75% al enraizar con NAA y ½ MS y 86% cuando se arraiga con IBA. El cultivo in vitro demuestra ser un método viable para propagar morera verde.Rubus brasiliensis e Rubus erythroclados são comumente conhecidas como amoreiras-verdes. Este trabalho teve como objetivo testar protocolos de micropropagação in vitro para amoreiras-verdes. O experimento de multiplicação foi conduzido utilizando BAP nas concentrações de 1 mg L-1, 2 mg L-1 e 3 mg L-1. O experimento de enraizamento foi realizado em meio ½ MS sem adição de hormônios, utilizando 0,1 mg L-1 de ANA ou 1,0 mg L-1 de AIB. A maior taxa de viabilidade dos explantes foi obtida com a propagação de R. brasiliensis com 1,0 mg L-1 de BAP. Explantes de enraizamento apresentaram taxas de sobrevivência superiores a 85%. A taxa de enraizamento em R. erythroclados foi de 87% enquanto em R. brasiliensis foi de 38%. As maiores taxas foram obtidas com 1,0 mg L-1 de AIB em R. erythroclados. A aclimatação atingiu taxas de sobrevivência de 75% quando enraizada com ANA e ½ MS e 86% quando enraizado com IBA. A cultura in vitro mostra-se um método viável para a propagação de amoreiras-verdes.Research, Society and Development2021-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1178810.33448/rsd-v10i1.11788Research, Society and Development; Vol. 10 No. 1; e29710111788Research, Society and Development; Vol. 10 Núm. 1; e29710111788Research, Society and Development; v. 10 n. 1; e297101117882525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/11788/10538Copyright (c) 2021 Thábata Cristina Faxina de Aguiar; Murilo Dalla Costa; Pedro Boff; Mari Inês Carissimi Boffhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAguiar, Thábata Cristina Faxina de Costa, Murilo Dalla Boff, PedroBoff, Mari Inês Carissimi 2021-02-20T21:19:23Zoai:ojs.pkp.sfu.ca:article/11788Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:33:31.542691Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Micropropagation on multiplication of native greenberries in South of Brazil Micropropagación en la multiplicación de moras verdes originarias del sur de Brasil Micropropagação na multiplicação de amoreiras-verdes nativas do Sul do Brasil |
title |
Micropropagation on multiplication of native greenberries in South of Brazil |
spellingShingle |
Micropropagation on multiplication of native greenberries in South of Brazil Aguiar, Thábata Cristina Faxina de Rubus brasiliensis Rubus erythroclados In vitro cultivation Domestication. Rubus brasiliensis Rubus erythroclados Cultivo in vitro Domesticación. Rubus brasiliensis Rubus erythroclados Cultivo in vitro Domesticação. |
title_short |
Micropropagation on multiplication of native greenberries in South of Brazil |
title_full |
Micropropagation on multiplication of native greenberries in South of Brazil |
title_fullStr |
Micropropagation on multiplication of native greenberries in South of Brazil |
title_full_unstemmed |
Micropropagation on multiplication of native greenberries in South of Brazil |
title_sort |
Micropropagation on multiplication of native greenberries in South of Brazil |
author |
Aguiar, Thábata Cristina Faxina de |
author_facet |
Aguiar, Thábata Cristina Faxina de Costa, Murilo Dalla Boff, Pedro Boff, Mari Inês Carissimi |
author_role |
author |
author2 |
Costa, Murilo Dalla Boff, Pedro Boff, Mari Inês Carissimi |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Aguiar, Thábata Cristina Faxina de Costa, Murilo Dalla Boff, Pedro Boff, Mari Inês Carissimi |
dc.subject.por.fl_str_mv |
Rubus brasiliensis Rubus erythroclados In vitro cultivation Domestication. Rubus brasiliensis Rubus erythroclados Cultivo in vitro Domesticación. Rubus brasiliensis Rubus erythroclados Cultivo in vitro Domesticação. |
topic |
Rubus brasiliensis Rubus erythroclados In vitro cultivation Domestication. Rubus brasiliensis Rubus erythroclados Cultivo in vitro Domesticación. Rubus brasiliensis Rubus erythroclados Cultivo in vitro Domesticação. |
description |
Rubus brasiliensis and Rubus erythroclados are commonly known as greenberries. This work aimed to test in vitro micropropagation protocols to greenberries. The multiplication experiment was conducted using BAP at concentrations 1 mg L-1, 2 mg L-1 and 3 mg L-1. The rooting experiment was carried out in ½ MS medium without the addition of hormones, using 0.1 mg L-1 NAA or 1.0 mg L-1 IBA. The highest rate of viability of explants was obtained with R. brasiliensis propagation using 1.0 mg L-1 of BAP. Rooting explants showed survival rates higher than 85%. Rooting rates in R. erythroclados was 87% while in R. brasiliensis it was 38%, The highest rates were obtained using 1.0 mg L-1 IBA in R. erythroclados.The acclimatization reached survival rates of 75% when rooted using NAA and ½ MS and 86% when rooted with IBA. In vitro culture shows to be a viable method to propagate greenberries. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/11788 10.33448/rsd-v10i1.11788 |
url |
https://rsdjournal.org/index.php/rsd/article/view/11788 |
identifier_str_mv |
10.33448/rsd-v10i1.11788 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/11788/10538 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 1; e29710111788 Research, Society and Development; Vol. 10 Núm. 1; e29710111788 Research, Society and Development; v. 10 n. 1; e29710111788 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
_version_ |
1797052824565055488 |