Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/23025 |
Resumo: | The Amazon is the largest tropical forest in the world and is home to around 20% of all the biodiversity on the planet, among the species present in the Amazon is Copaifera langsdorffii, exploited mainly for the extraction of oil-resin and wood, often in ways incorrect, which can cause the loss of genetic variability. The aim of this study was to evaluate the genetic structure and diversity among individuals of C. langsdorffii located in Mato Grosso, Brazil, using ISSR markers. We sampled leaves from 27 adult individuals of C. langsdorffii, whose total genomic DNA was extracted. A total of 12 ISSR primers were used for the molecular characterization of the individuals. A grouping analysis was performed using the unweighted pair group method, Bayesian analysis and characterized by the genetic diversity. The genetic diversity among and within the groups was demonstrated by the AMOVA. As a result, 106 fragments were amplified and 98.11% were polymorphic. The polymorphic information content of each primer ranged from 0.45 to 0.81. The dendrogram showed the formation of 4 distinct groups. The greatest genetic variability is found within the groups and not between them. The percentage of polymorphism, genetic dissimilarity values and genetic diversity indexes indicate that there is high genetic variability among Copaifera langsdorffii individuals, suggesting that ISSR primers were efficient in detecting polymorphism in this species and that the individuals have potential for compose programs aimed at the preservation of the species and the ability to integrate germplasm banks. |
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Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markersDiversidad y estructura genética entre individuos de Copaifera langsdorffii Desf. de Mato Grosso, Amazonía Brasileira, utilizando marcadores ISSRDiversidade e estrutura genética entre indivíduos de Copaifera langsdorffii Desf. de Mato Grosso, Amazônia Brasileira, por meio de marcadores ISSRBiodiversityMolecular characterizationCopaibaMolecular markerGenetic variability.BiodiversidadCaracterización molecularCopaibaMarcador molecularVariabilidad genética.BiodiversidadeCaracterização molecularCopaíbaMarcador molecularVariabilidade genética.The Amazon is the largest tropical forest in the world and is home to around 20% of all the biodiversity on the planet, among the species present in the Amazon is Copaifera langsdorffii, exploited mainly for the extraction of oil-resin and wood, often in ways incorrect, which can cause the loss of genetic variability. The aim of this study was to evaluate the genetic structure and diversity among individuals of C. langsdorffii located in Mato Grosso, Brazil, using ISSR markers. We sampled leaves from 27 adult individuals of C. langsdorffii, whose total genomic DNA was extracted. A total of 12 ISSR primers were used for the molecular characterization of the individuals. A grouping analysis was performed using the unweighted pair group method, Bayesian analysis and characterized by the genetic diversity. The genetic diversity among and within the groups was demonstrated by the AMOVA. As a result, 106 fragments were amplified and 98.11% were polymorphic. The polymorphic information content of each primer ranged from 0.45 to 0.81. The dendrogram showed the formation of 4 distinct groups. The greatest genetic variability is found within the groups and not between them. The percentage of polymorphism, genetic dissimilarity values and genetic diversity indexes indicate that there is high genetic variability among Copaifera langsdorffii individuals, suggesting that ISSR primers were efficient in detecting polymorphism in this species and that the individuals have potential for compose programs aimed at the preservation of the species and the ability to integrate germplasm banks.La Amazonía es el bosque tropical más grande del mundo y alberga alrededor del 20% de toda la biodiversidad del planeta, entre las especies presentes en la Amazonía se encuentra Copaifera langsdorffii, explotada principalmente para la extracción de aceite-resina y madera, a menudo de forma incorrecta, lo que puede provocar la pérdida de variabilidad genética. El objetivo fue evaluar la estructura y diversidad genética entre individuos de C. langsdorffii ubicados en la Amazonía (Mato Grosso, Brasil), utilizando marcadores ISSR. Muestreamos hojas de 27 individuos adultos de C. langsdorffii, cuyo ADN genómico total fue extraído. Se utilizaron doce cebadores ISSR para la caracterización molecular. Se realizó análisis de conglomerados, el análisis bayesiano y caracterización de la variabilidad genética. La diversidad genética entre y dentro de los grupos se demostró a partir del AMOVA. Se amplificaron 106 fragmentos, 98.11% de los cuales eran polimórficos. El contenido de información polimórfica de cada cebador varió entre 0.45 y 0.81. El dendrograma mostró la formación de 4 grupos distintos. La mayor variabilidad genética se encuentra dentro de los grupos y no entre ellos. El porcentaje de polimorfismo, los valores de disimilitud genética y los índices de diversidad genética señalan que existe una alta variabilidad genética entre los individuos de Copaifera langsdorffii, lo que sugiere que los cebadores ISSR demostraron ser eficientes en la detección de polimorfismo en esta especie y que el los individuos tienen potencial para componer programas dirigidos a la preservación de la especie y la capacidad de integrar bancos de germoplasma.A Amazônia é a maior Floresta tropical do mundo e abriga cerca de 20% de toda a biodiversidade do planeta, dentre as espécies presentes na Amazônia está a Copaifera langsdorffii, explorada principalmente para extração de óleo-resina e madeira, muitas vezes de forma incorreta, o que pode ocasionar a perda de variabilidade genética. O objetivo deste trabalho foi avaliar a diversidade e estrutura genética entre indivíduos de C. langsdorffii localizada da Amazônia Mato-Grossense, por meio de marcadores ISSR. Foram amostradas folhas de 27 indivíduos adultos de C. langsdorffii, cujo DNA genômico total foi extraído. Foram utilizados 12 primers ISSR para caracterização molecular dos indivíduos. Foram realizadas análise de agrupamento, análise bayesiana, e caracterização da variabilidade genética. A diversidade genética entre e dentro dos grupos foi demonstrada a partir da AMOVA. Foram amplificados 106 fragmentos, sendo 98,11% polimórficos. O conteúdo de informação polimórfica de cada primer variou entre 0,45 e 0,81. O dendrograma mostrou a formação de 4 grupos distintos. A maior variabilidade genética encontra-se dentro dos grupos e não entre eles. O percentual de polimorfismo, valores de dissimilaridade genética e índices de diversidade genética apontam que há alta variabilidade genética entre os indivíduos de Copaifera langsdorffii, sugerindo que os primers ISSR se mostraram eficientes na detecção de polimorfismo nesta espécie e que os indivíduos apresentam potencial para compor programas que visem a preservação da espécie e capacidade de integrar bancos de germoplasma.Research, Society and Development2021-12-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2302510.33448/rsd-v10i16.23025Research, Society and Development; Vol. 10 No. 16; e187101623025Research, Society and Development; Vol. 10 Núm. 16; e187101623025Research, Society and Development; v. 10 n. 16; e1871016230252525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/23025/20847Copyright (c) 2021 Daniele Paula Maltezo; Julliane Dutra Medeiros; Ana Aparecida Bandini Rossihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMaltezo, Daniele PaulaMedeiros, Julliane DutraRossi, Ana Aparecida Bandini 2021-12-20T11:03:07Zoai:ojs.pkp.sfu.ca:article/23025Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:01.143641Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers Diversidad y estructura genética entre individuos de Copaifera langsdorffii Desf. de Mato Grosso, Amazonía Brasileira, utilizando marcadores ISSR Diversidade e estrutura genética entre indivíduos de Copaifera langsdorffii Desf. de Mato Grosso, Amazônia Brasileira, por meio de marcadores ISSR |
title |
Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers |
spellingShingle |
Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers Maltezo, Daniele Paula Biodiversity Molecular characterization Copaiba Molecular marker Genetic variability. Biodiversidad Caracterización molecular Copaiba Marcador molecular Variabilidad genética. Biodiversidade Caracterização molecular Copaíba Marcador molecular Variabilidade genética. |
title_short |
Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers |
title_full |
Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers |
title_fullStr |
Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers |
title_full_unstemmed |
Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers |
title_sort |
Genetic structure and diversity among individuals of Copaifera langsdorffii Desf. from Mato Grosso, Brazilian Amazon, using ISSR markers |
author |
Maltezo, Daniele Paula |
author_facet |
Maltezo, Daniele Paula Medeiros, Julliane Dutra Rossi, Ana Aparecida Bandini |
author_role |
author |
author2 |
Medeiros, Julliane Dutra Rossi, Ana Aparecida Bandini |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Maltezo, Daniele Paula Medeiros, Julliane Dutra Rossi, Ana Aparecida Bandini |
dc.subject.por.fl_str_mv |
Biodiversity Molecular characterization Copaiba Molecular marker Genetic variability. Biodiversidad Caracterización molecular Copaiba Marcador molecular Variabilidad genética. Biodiversidade Caracterização molecular Copaíba Marcador molecular Variabilidade genética. |
topic |
Biodiversity Molecular characterization Copaiba Molecular marker Genetic variability. Biodiversidad Caracterización molecular Copaiba Marcador molecular Variabilidad genética. Biodiversidade Caracterização molecular Copaíba Marcador molecular Variabilidade genética. |
description |
The Amazon is the largest tropical forest in the world and is home to around 20% of all the biodiversity on the planet, among the species present in the Amazon is Copaifera langsdorffii, exploited mainly for the extraction of oil-resin and wood, often in ways incorrect, which can cause the loss of genetic variability. The aim of this study was to evaluate the genetic structure and diversity among individuals of C. langsdorffii located in Mato Grosso, Brazil, using ISSR markers. We sampled leaves from 27 adult individuals of C. langsdorffii, whose total genomic DNA was extracted. A total of 12 ISSR primers were used for the molecular characterization of the individuals. A grouping analysis was performed using the unweighted pair group method, Bayesian analysis and characterized by the genetic diversity. The genetic diversity among and within the groups was demonstrated by the AMOVA. As a result, 106 fragments were amplified and 98.11% were polymorphic. The polymorphic information content of each primer ranged from 0.45 to 0.81. The dendrogram showed the formation of 4 distinct groups. The greatest genetic variability is found within the groups and not between them. The percentage of polymorphism, genetic dissimilarity values and genetic diversity indexes indicate that there is high genetic variability among Copaifera langsdorffii individuals, suggesting that ISSR primers were efficient in detecting polymorphism in this species and that the individuals have potential for compose programs aimed at the preservation of the species and the ability to integrate germplasm banks. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23025 10.33448/rsd-v10i16.23025 |
url |
https://rsdjournal.org/index.php/rsd/article/view/23025 |
identifier_str_mv |
10.33448/rsd-v10i16.23025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/23025/20847 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Daniele Paula Maltezo; Julliane Dutra Medeiros; Ana Aparecida Bandini Rossi https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Daniele Paula Maltezo; Julliane Dutra Medeiros; Ana Aparecida Bandini Rossi https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 16; e187101623025 Research, Society and Development; Vol. 10 Núm. 16; e187101623025 Research, Society and Development; v. 10 n. 16; e187101623025 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052696544411648 |