Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/19471 |
Resumo: | Background: Cervical cancer is the fourth most common cancer among women worldwide. In order to make the diagnosis earlier and more accurate, increase the prospect of using microRNA-29b, which targets matrix metalloproteinase-2 (MMP-2), proteolytic enzymes that play a significant role in the degradation of the important extracellular matrix in progression of cervical carcinogenic lesions. Objectives: The aim of this study was to evaluate the levels of expression of microRNA-29b at all histological levels of carcinogenesis and its relationship with MMP-2 in this type of cancer. Methods: RNA was extracted from eighteen paraffin-embedded biopsy samples diagnosed with carcinoma and 19 considerable healthy samples, after which a real-time PCR technician was performed for expression analysis. Results: Our results show underexpression of microRNA-29b and MMP-2. This decrease in expression was statistically significant and differentiated in the histopathological subtypes. In addition, it has been shown that MMP-2 is directly regulated by microRNA-29b. No other studies were found that performed the same analysis on cervical cancers. Conclusion: Our results indicate a potential diagnosis of microRNA-29b and MMP-2 for lesions and cervical cancer, and show a direct association between these molecules. |
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Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic processPerfil de expresión del microARN-29b y su acción sobre la metaloproteinasa 2 de la matriz (MMP-2) em el proceso carcinogénico cervicalPerfil de expressão do microRNA-29b e sua ação sobre a metaloproteinase de matriz 2 (MMP-2) no processo da carcinogênese cervicalCáncer de cuello uterinomicroARN-29bBiomarcadorMetaloproteinasa de matriz 2.Cervical CancermicroRNA-29bBiomarkerMatrix metalloproteinase 2.Câncer cervicalmicroRNA-29bBiomarcadorMetaloproteinase de matriz 2.Background: Cervical cancer is the fourth most common cancer among women worldwide. In order to make the diagnosis earlier and more accurate, increase the prospect of using microRNA-29b, which targets matrix metalloproteinase-2 (MMP-2), proteolytic enzymes that play a significant role in the degradation of the important extracellular matrix in progression of cervical carcinogenic lesions. Objectives: The aim of this study was to evaluate the levels of expression of microRNA-29b at all histological levels of carcinogenesis and its relationship with MMP-2 in this type of cancer. Methods: RNA was extracted from eighteen paraffin-embedded biopsy samples diagnosed with carcinoma and 19 considerable healthy samples, after which a real-time PCR technician was performed for expression analysis. Results: Our results show underexpression of microRNA-29b and MMP-2. This decrease in expression was statistically significant and differentiated in the histopathological subtypes. In addition, it has been shown that MMP-2 is directly regulated by microRNA-29b. No other studies were found that performed the same analysis on cervical cancers. Conclusion: Our results indicate a potential diagnosis of microRNA-29b and MMP-2 for lesions and cervical cancer, and show a direct association between these molecules.Antecedentes: El cáncer de cuello uterino es el cuarto cáncer más común entre las mujeres en todo el mundo. Para hacer el diagnóstico más temprano y más preciso, aumente la posibilidad de utilizar microARN-29b, que se dirige a la metaloproteinasa-2 de la matriz (MMP-2), enzimas proteolíticas que desempeñan un papel importante en la degradación de la matriz extracelular en la progresión de lesiones cancerígenas cervicales. Objetivos: El objetivo de este estudio fue evaluar los niveles de expresión del microARN-29b en todos los niveles histológicos de carcinogénesis y su relación con MMP-2 en este tipo de cáncer. Métodos: Se extrajo ARN de dieciocho muestras de biopsia incluidas en parafina diagnosticadas con carcinoma y 19 muestras sanas considerables, tras lo cual se realizó un técnico de PCR en tiempo real para el análisis de expresión. Resultados: Nuestros resultados muestran una subexpresión de microARN-29b y MMP-2. Esta disminución de la expresión fue estadísticamente significativa y diferenciada en los subtipos histopatológicos. Además, se ha demostrado que MMP-2 está regulada directamente por microARN-29b. No se encontraron otros estudios que realizaran el mismo análisis sobre los cánceres de cuello uterino. Conclusión: Nuestros resultados indican un diagnóstico potencial de microARN-29b y MMP-2 para lesiones y cáncer de cuello uterino, y muestran una asociación directa entre estas moléculas.Introdução: O câncer cervical é o quarto tipo de câncer mais comum entre as mulheres em todo o mundo. A fim de tornar o diagnóstico mais precoce e preciso, aumenta a perspectiva de utilização de microRNA-29b, que tem como alvo a metaloproteinase-2 da matriz (MMP-2), enzimas proteolíticas que desempenham significativo papel na degradação da matriz extracelular e consequente progressão das lesões cancerígenas cervicais. Objetivos: O objetivo deste estudo foi avaliar os níveis de expressão do microRNA-29b em todos os níveis histológicos da carcinogênese cervical e sua relação com a MMP-2 nesse tipo de câncer. Métodos: o RNA foi extraído de dezoito amostras de biópsia embebidas em parafina com diagnóstico de carcinoma e 19 amostras consideradas saudáveis, foi realizada a técnica de PCR em tempo real para análise de expressão do microRNA-29b. Resultados: Nossos resultados mostram subexpressão de microRNA-29b e MMP-2. Essa diminuição da expressão foi estatisticamente significativa e diferenciada nos subtipos histopatológicos. Além disso, foi demonstrado que a MMP-2 é regulada diretamente pelo microRNA-29b. Não foram encontrados outros estudos que realizassem a mesma análise em cânceres cervicais. Conclusão: Nossos resultados indicam um potencial diagnóstico de microRNA-29b e MMP-2 para lesões e câncer cervical, e mostram uma associação direta entre essas moléculas.Research, Society and Development2021-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1947110.33448/rsd-v10i11.19471Research, Society and Development; Vol. 10 No. 11; e372101119471Research, Society and Development; Vol. 10 Núm. 11; e372101119471Research, Society and Development; v. 10 n. 11; e3721011194712525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/19471/17630Copyright (c) 2021 Giwellington Silva Albuquerque; Renata Santos de Oliveira; Jônatas André da Silva Santos; Miquéias Pereira Lima do Amaral; Juliana Maria de Souza; Bárbara de Oliveira Silva; Jacinto da Costa Silva Neto; Maria Tereza Cartaxo Munizhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAlbuquerque, Giwellington Silva Oliveira, Renata Santos de Santos, Jônatas André da Silva Amaral, Miquéias Pereira Lima do Souza, Juliana Maria de Silva, Bárbara de Oliveira Silva Neto, Jacinto da Costa Muniz, Maria Tereza Cartaxo 2021-10-23T19:01:11Zoai:ojs.pkp.sfu.ca:article/19471Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:39:21.356705Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process Perfil de expresión del microARN-29b y su acción sobre la metaloproteinasa 2 de la matriz (MMP-2) em el proceso carcinogénico cervical Perfil de expressão do microRNA-29b e sua ação sobre a metaloproteinase de matriz 2 (MMP-2) no processo da carcinogênese cervical |
| title |
Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process |
| spellingShingle |
Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process Albuquerque, Giwellington Silva Cáncer de cuello uterino microARN-29b Biomarcador Metaloproteinasa de matriz 2. Cervical Cancer microRNA-29b Biomarker Matrix metalloproteinase 2. Câncer cervical microRNA-29b Biomarcador Metaloproteinase de matriz 2. |
| title_short |
Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process |
| title_full |
Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process |
| title_fullStr |
Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process |
| title_full_unstemmed |
Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process |
| title_sort |
Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process |
| author |
Albuquerque, Giwellington Silva |
| author_facet |
Albuquerque, Giwellington Silva Oliveira, Renata Santos de Santos, Jônatas André da Silva Amaral, Miquéias Pereira Lima do Souza, Juliana Maria de Silva, Bárbara de Oliveira Silva Neto, Jacinto da Costa Muniz, Maria Tereza Cartaxo |
| author_role |
author |
| author2 |
Oliveira, Renata Santos de Santos, Jônatas André da Silva Amaral, Miquéias Pereira Lima do Souza, Juliana Maria de Silva, Bárbara de Oliveira Silva Neto, Jacinto da Costa Muniz, Maria Tereza Cartaxo |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Albuquerque, Giwellington Silva Oliveira, Renata Santos de Santos, Jônatas André da Silva Amaral, Miquéias Pereira Lima do Souza, Juliana Maria de Silva, Bárbara de Oliveira Silva Neto, Jacinto da Costa Muniz, Maria Tereza Cartaxo |
| dc.subject.por.fl_str_mv |
Cáncer de cuello uterino microARN-29b Biomarcador Metaloproteinasa de matriz 2. Cervical Cancer microRNA-29b Biomarker Matrix metalloproteinase 2. Câncer cervical microRNA-29b Biomarcador Metaloproteinase de matriz 2. |
| topic |
Cáncer de cuello uterino microARN-29b Biomarcador Metaloproteinasa de matriz 2. Cervical Cancer microRNA-29b Biomarker Matrix metalloproteinase 2. Câncer cervical microRNA-29b Biomarcador Metaloproteinase de matriz 2. |
| description |
Background: Cervical cancer is the fourth most common cancer among women worldwide. In order to make the diagnosis earlier and more accurate, increase the prospect of using microRNA-29b, which targets matrix metalloproteinase-2 (MMP-2), proteolytic enzymes that play a significant role in the degradation of the important extracellular matrix in progression of cervical carcinogenic lesions. Objectives: The aim of this study was to evaluate the levels of expression of microRNA-29b at all histological levels of carcinogenesis and its relationship with MMP-2 in this type of cancer. Methods: RNA was extracted from eighteen paraffin-embedded biopsy samples diagnosed with carcinoma and 19 considerable healthy samples, after which a real-time PCR technician was performed for expression analysis. Results: Our results show underexpression of microRNA-29b and MMP-2. This decrease in expression was statistically significant and differentiated in the histopathological subtypes. In addition, it has been shown that MMP-2 is directly regulated by microRNA-29b. No other studies were found that performed the same analysis on cervical cancers. Conclusion: Our results indicate a potential diagnosis of microRNA-29b and MMP-2 for lesions and cervical cancer, and show a direct association between these molecules. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-09-04 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/19471 10.33448/rsd-v10i11.19471 |
| url |
https://rsdjournal.org/index.php/rsd/article/view/19471 |
| identifier_str_mv |
10.33448/rsd-v10i11.19471 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://rsdjournal.org/index.php/rsd/article/view/19471/17630 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Research, Society and Development |
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Research, Society and Development |
| dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 11; e372101119471 Research, Society and Development; Vol. 10 Núm. 11; e372101119471 Research, Society and Development; v. 10 n. 11; e372101119471 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052754445729792 |