Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/19382 |
Resumo: | Aim: Isolation and chemical characterization of a substance extracted from leaves of the species Argemone mexicana L. was performed. In addition, the antibacterial activity and cell viability of this substance was determined. Method: The isolation of the substance was performed by classical liquid layer chromatography, for the chemical characterization were made Infrared and Nuclear Magnetic Resonance spectroscopy. Antibacterial activity was determined by the broth microdilution method to determine the minimum inhibitory concentration of the substance. The cell viability assay was performed with the 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2Htetrazoliumbromide colorimetric method using J774 macrophage strains. Results: The substance isolated from the species Argemone mexicana L. was identified as berberine alkaloid. The ability to maintain viable cells of the substance was positive, as it not only maintained viable cells but also promoted proliferation of treated macrophages. This alkaloid demonstrated promising antibacterial activity against Gram positive and negative strains, mainly against Pseudomonas aeruginosa at a concentration of 62,5µg/ml. Conclusion: Berberine's ability to maintain and proliferate cells and inhibit bacterial growth suggests its potential for future antimicrobial treatment and in conditions that require stimulation of cell proliferation. |
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Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approachCaracterización química de sustancia aislada de hojas de Argemone mexicana L. y ensayos de viabilidad celular y actividad antibacteriana: enfoque in vitro Caracterização química de substância isolada das folhas de Argemone mexicana L. e ensaios de viabilidade celular e atividade antibacteriana: abordagem in vitro Argemone mexicana L.Anti-bacterial agentsCell survivalBerberine.Argemone mexicana L.AntibacterianosSupervivencia celularBerberine.Argemone mexicana L.AntibacterianosSobrevivência celularBerberina.Aim: Isolation and chemical characterization of a substance extracted from leaves of the species Argemone mexicana L. was performed. In addition, the antibacterial activity and cell viability of this substance was determined. Method: The isolation of the substance was performed by classical liquid layer chromatography, for the chemical characterization were made Infrared and Nuclear Magnetic Resonance spectroscopy. Antibacterial activity was determined by the broth microdilution method to determine the minimum inhibitory concentration of the substance. The cell viability assay was performed with the 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2Htetrazoliumbromide colorimetric method using J774 macrophage strains. Results: The substance isolated from the species Argemone mexicana L. was identified as berberine alkaloid. The ability to maintain viable cells of the substance was positive, as it not only maintained viable cells but also promoted proliferation of treated macrophages. This alkaloid demonstrated promising antibacterial activity against Gram positive and negative strains, mainly against Pseudomonas aeruginosa at a concentration of 62,5µg/ml. Conclusion: Berberine's ability to maintain and proliferate cells and inhibit bacterial growth suggests its potential for future antimicrobial treatment and in conditions that require stimulation of cell proliferation.Objetivo: Se realizó el aislamiento y caracterización química de una sustancia extraída de hojas de la especie Argemone mexicana L. Además, se determinó la actividad antibacteriana y la viabilidad celular de esta sustancia. Método: El aislamiento de la sustancia se realizó mediante cromatografía en capa líquida clásica, para la caracterización química se realizó espectroscopía de Infrarrojos y Resonancia Magnética Nuclear. La actividad antibacteriana se determinó mediante el método de microdilución en caldo para determinar la concentración inhibitoria mínima de la sustancia. El ensayo de viabilidad celular se realizó con el método colorimétrico de bromuro de 3- (4,5-dimetil-2-tiazolil) -2,5-difenil-2Htetrazolio utilizando cepas de macrófagos J774. Resultados: La sustancia aislada de la especie Argemone mexicana L. fue identificada como alcaloide berberina. La capacidad de mantener células viables de la sustancia fue positiva, ya que no solo mantuvo células viables sino que también promovió la proliferación de macrófagos tratados. Este alcaloide demostró una actividad antibacteriana prometedora contra cepas Gram positivas y negativas, principalmente contra Pseudomonas aeruginosa a una concentración de 62,5 µg / ml. Conclusión: La capacidad de la berberina para mantener y proliferar células e inhibir el crecimiento bacteriano sugiere su potencial para futuros tratamientos antimicrobianos y en condiciones que requieren estimulación de la proliferación celular.Objetivo: O isolamento e caracterização química de uma substância extraída das folhas da espécie Argemone mexicana L. foi realizado. Além disso, a atividade antibacteriana e de viabilidade celular desta substância foi determinada. Métodos: O isolamento da substância foi realizado por meio da cromatografia em camada líquida clássica, para a caracterização química foram feitas espectroscopia no Infravermelho e de Ressonância Magnética Nuclear. A atividade antibacteriana foi determinada por meio do metódo de microdiluição em caldo a fim de determinar a concentração inibitória mínima da substância. O ensaio de viabilidade celular foi realizado com o método colorimétrico do 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2Htetrazoliumbromide utilizando linhagens de macrófagos J774. Resultados: A substância isolada da espécie Argemone mexicana L. foi identificada como sendo o alcaloide berberina. A capacidade de manter células viáveis da substância foi positiva, visto que ela não só manteve as células viáveis como também promoveu a proliferação dos macrófagos tratados. Este alcaloide exibiu promissora atividade antibacteriana frente a cepas Gram positivas e negativas, principalmente frente a espécie Pseudomonas aeruginosa numa concentração de 62,5µg/ml. Conclusão: A capacidade da berberina em manter e proliferar células e inibir o crescimento bacteriano sugere seu potencial para tratamento futuro como antimicrobiano e em patologias que necessitam de estímulo na proliferação celular.Research, Society and Development2021-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1938210.33448/rsd-v10i11.19382Research, Society and Development; Vol. 10 No. 11; e65101119382Research, Society and Development; Vol. 10 Núm. 11; e65101119382Research, Society and Development; v. 10 n. 11; e651011193822525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/19382/17256Copyright (c) 2021 Maria Gabriella Silva Araujo; Regina Célia Sales Santos; Lucianna Costa de Almeida Barbosa; Wanderlei Barbosa Dos Santos; Edeildo Ferreira Silva Junior; João Xavier de Araújo Júniorhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Araujo, Maria Gabriella SilvaSantos, Regina Célia Sales Barbosa, Lucianna Costa de AlmeidaSantos, Wanderlei Barbosa Dos Silva Junior, Edeildo Ferreira Araújo Júnior, João Xavier de2021-10-23T19:01:11Zoai:ojs.pkp.sfu.ca:article/19382Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:39:17.251754Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach Caracterización química de sustancia aislada de hojas de Argemone mexicana L. y ensayos de viabilidad celular y actividad antibacteriana: enfoque in vitro Caracterização química de substância isolada das folhas de Argemone mexicana L. e ensaios de viabilidade celular e atividade antibacteriana: abordagem in vitro |
title |
Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach |
spellingShingle |
Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach Araujo, Maria Gabriella Silva Argemone mexicana L. Anti-bacterial agents Cell survival Berberine. Argemone mexicana L. Antibacterianos Supervivencia celular Berberine. Argemone mexicana L. Antibacterianos Sobrevivência celular Berberina. |
title_short |
Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach |
title_full |
Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach |
title_fullStr |
Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach |
title_full_unstemmed |
Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach |
title_sort |
Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach |
author |
Araujo, Maria Gabriella Silva |
author_facet |
Araujo, Maria Gabriella Silva Santos, Regina Célia Sales Barbosa, Lucianna Costa de Almeida Santos, Wanderlei Barbosa Dos Silva Junior, Edeildo Ferreira Araújo Júnior, João Xavier de |
author_role |
author |
author2 |
Santos, Regina Célia Sales Barbosa, Lucianna Costa de Almeida Santos, Wanderlei Barbosa Dos Silva Junior, Edeildo Ferreira Araújo Júnior, João Xavier de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Araujo, Maria Gabriella Silva Santos, Regina Célia Sales Barbosa, Lucianna Costa de Almeida Santos, Wanderlei Barbosa Dos Silva Junior, Edeildo Ferreira Araújo Júnior, João Xavier de |
dc.subject.por.fl_str_mv |
Argemone mexicana L. Anti-bacterial agents Cell survival Berberine. Argemone mexicana L. Antibacterianos Supervivencia celular Berberine. Argemone mexicana L. Antibacterianos Sobrevivência celular Berberina. |
topic |
Argemone mexicana L. Anti-bacterial agents Cell survival Berberine. Argemone mexicana L. Antibacterianos Supervivencia celular Berberine. Argemone mexicana L. Antibacterianos Sobrevivência celular Berberina. |
description |
Aim: Isolation and chemical characterization of a substance extracted from leaves of the species Argemone mexicana L. was performed. In addition, the antibacterial activity and cell viability of this substance was determined. Method: The isolation of the substance was performed by classical liquid layer chromatography, for the chemical characterization were made Infrared and Nuclear Magnetic Resonance spectroscopy. Antibacterial activity was determined by the broth microdilution method to determine the minimum inhibitory concentration of the substance. The cell viability assay was performed with the 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2Htetrazoliumbromide colorimetric method using J774 macrophage strains. Results: The substance isolated from the species Argemone mexicana L. was identified as berberine alkaloid. The ability to maintain viable cells of the substance was positive, as it not only maintained viable cells but also promoted proliferation of treated macrophages. This alkaloid demonstrated promising antibacterial activity against Gram positive and negative strains, mainly against Pseudomonas aeruginosa at a concentration of 62,5µg/ml. Conclusion: Berberine's ability to maintain and proliferate cells and inhibit bacterial growth suggests its potential for future antimicrobial treatment and in conditions that require stimulation of cell proliferation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/19382 10.33448/rsd-v10i11.19382 |
url |
https://rsdjournal.org/index.php/rsd/article/view/19382 |
identifier_str_mv |
10.33448/rsd-v10i11.19382 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/19382/17256 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 11; e65101119382 Research, Society and Development; Vol. 10 Núm. 11; e65101119382 Research, Society and Development; v. 10 n. 11; e65101119382 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052687568601088 |