Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach

Detalhes bibliográficos
Autor(a) principal: Araujo, Maria Gabriella Silva
Data de Publicação: 2021
Outros Autores: Santos, Regina Célia Sales, Barbosa, Lucianna Costa de Almeida, Santos, Wanderlei Barbosa Dos, Silva Junior, Edeildo Ferreira, Araújo Júnior, João Xavier de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/19382
Resumo: Aim: Isolation and chemical characterization of a substance extracted from leaves of the species Argemone mexicana L. was performed. In addition, the antibacterial activity and cell viability of this substance was determined. Method: The isolation of the substance was performed by classical liquid layer chromatography, for the chemical characterization were made Infrared and Nuclear Magnetic Resonance spectroscopy. Antibacterial activity was determined by the broth microdilution method to determine the minimum inhibitory concentration of the substance. The cell viability assay was performed with the 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2Htetrazoliumbromide colorimetric method using J774 macrophage strains. Results: The substance isolated from the species Argemone mexicana L. was identified as berberine alkaloid. The ability to maintain viable cells of the substance was positive, as it not only maintained viable cells but also promoted proliferation of treated macrophages. This alkaloid demonstrated promising antibacterial activity against Gram positive and negative strains, mainly against Pseudomonas aeruginosa at a concentration of 62,5µg/ml. Conclusion: Berberine's ability to maintain and proliferate cells and inhibit bacterial growth suggests its potential for future antimicrobial treatment and in conditions that require stimulation of cell proliferation.
id UNIFEI_9873f72d02f2ba2a7abf22bb44e84ab6
oai_identifier_str oai:ojs.pkp.sfu.ca:article/19382
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approachCaracterización química de sustancia aislada de hojas de Argemone mexicana L. y ensayos de viabilidad celular y actividad antibacteriana: enfoque in vitro Caracterização química de substância isolada das folhas de Argemone mexicana L. e ensaios de viabilidade celular e atividade antibacteriana: abordagem in vitro Argemone mexicana L.Anti-bacterial agentsCell survivalBerberine.Argemone mexicana L.AntibacterianosSupervivencia celularBerberine.Argemone mexicana L.AntibacterianosSobrevivência celularBerberina.Aim: Isolation and chemical characterization of a substance extracted from leaves of the species Argemone mexicana L. was performed. In addition, the antibacterial activity and cell viability of this substance was determined. Method: The isolation of the substance was performed by classical liquid layer chromatography, for the chemical characterization were made Infrared and Nuclear Magnetic Resonance spectroscopy. Antibacterial activity was determined by the broth microdilution method to determine the minimum inhibitory concentration of the substance. The cell viability assay was performed with the 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2Htetrazoliumbromide colorimetric method using J774 macrophage strains. Results: The substance isolated from the species Argemone mexicana L. was identified as berberine alkaloid. The ability to maintain viable cells of the substance was positive, as it not only maintained viable cells but also promoted proliferation of treated macrophages. This alkaloid demonstrated promising antibacterial activity against Gram positive and negative strains, mainly against Pseudomonas aeruginosa at a concentration of 62,5µg/ml. Conclusion: Berberine's ability to maintain and proliferate cells and inhibit bacterial growth suggests its potential for future antimicrobial treatment and in conditions that require stimulation of cell proliferation.Objetivo: Se realizó el aislamiento y caracterización química de una sustancia extraída de hojas de la especie Argemone mexicana L. Además, se determinó la actividad antibacteriana y la viabilidad celular de esta sustancia. Método: El aislamiento de la sustancia se realizó mediante cromatografía en capa líquida clásica, para la caracterización química se realizó espectroscopía de Infrarrojos y Resonancia Magnética Nuclear. La actividad antibacteriana se determinó mediante el método de microdilución en caldo para determinar la concentración inhibitoria mínima de la sustancia. El ensayo de viabilidad celular se realizó con el método colorimétrico de bromuro de 3- (4,5-dimetil-2-tiazolil) -2,5-difenil-2Htetrazolio utilizando cepas de macrófagos J774. Resultados: La sustancia aislada de la especie Argemone mexicana L. fue identificada como alcaloide berberina. La capacidad de mantener células viables de la sustancia fue positiva, ya que no solo mantuvo células viables sino que también promovió la proliferación de macrófagos tratados. Este alcaloide demostró una actividad antibacteriana prometedora contra cepas Gram positivas y negativas, principalmente contra Pseudomonas aeruginosa a una concentración de 62,5 µg / ml. Conclusión: La capacidad de la berberina para mantener y proliferar células e inhibir el crecimiento bacteriano sugiere su potencial para futuros tratamientos antimicrobianos y en condiciones que requieren estimulación de la proliferación celular.Objetivo: O isolamento e caracterização química de uma substância extraída das folhas da espécie Argemone mexicana L. foi realizado. Além disso, a atividade antibacteriana e de viabilidade celular desta substância foi determinada. Métodos: O isolamento da substância foi realizado por meio da cromatografia em camada líquida clássica, para a caracterização química foram feitas espectroscopia no Infravermelho e de Ressonância Magnética Nuclear. A atividade antibacteriana foi determinada por meio do metódo de microdiluição em caldo a fim de determinar a concentração inibitória mínima da substância. O ensaio de viabilidade celular foi realizado com o método colorimétrico do 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2Htetrazoliumbromide utilizando linhagens de macrófagos J774. Resultados: A substância isolada da espécie Argemone mexicana L. foi identificada como sendo o alcaloide berberina. A capacidade de manter células viáveis da substância foi positiva, visto que ela não só manteve as células viáveis como também promoveu a proliferação dos macrófagos tratados. Este alcaloide exibiu promissora atividade antibacteriana frente a cepas Gram positivas e negativas, principalmente frente a espécie Pseudomonas aeruginosa numa concentração de 62,5µg/ml. Conclusão: A capacidade da berberina em manter e proliferar células e inibir o crescimento bacteriano sugere seu potencial para tratamento futuro como antimicrobiano e em patologias que necessitam de estímulo na proliferação celular.Research, Society and Development2021-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1938210.33448/rsd-v10i11.19382Research, Society and Development; Vol. 10 No. 11; e65101119382Research, Society and Development; Vol. 10 Núm. 11; e65101119382Research, Society and Development; v. 10 n. 11; e651011193822525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/19382/17256Copyright (c) 2021 Maria Gabriella Silva Araujo; Regina Célia Sales Santos; Lucianna Costa de Almeida Barbosa; Wanderlei Barbosa Dos Santos; Edeildo Ferreira Silva Junior; João Xavier de Araújo Júniorhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Araujo, Maria Gabriella SilvaSantos, Regina Célia Sales Barbosa, Lucianna Costa de AlmeidaSantos, Wanderlei Barbosa Dos Silva Junior, Edeildo Ferreira Araújo Júnior, João Xavier de2021-10-23T19:01:11Zoai:ojs.pkp.sfu.ca:article/19382Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:39:17.251754Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
Caracterización química de sustancia aislada de hojas de Argemone mexicana L. y ensayos de viabilidad celular y actividad antibacteriana: enfoque in vitro
Caracterização química de substância isolada das folhas de Argemone mexicana L. e ensaios de viabilidade celular e atividade antibacteriana: abordagem in vitro
title Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
spellingShingle Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
Araujo, Maria Gabriella Silva
Argemone mexicana L.
Anti-bacterial agents
Cell survival
Berberine.
Argemone mexicana L.
Antibacterianos
Supervivencia celular
Berberine.
Argemone mexicana L.
Antibacterianos
Sobrevivência celular
Berberina.
title_short Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
title_full Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
title_fullStr Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
title_full_unstemmed Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
title_sort Chemical characterization of isolated substance of Argemone mexicana L. leaves and cell viability assays and antibacterial activity: in vitro approach
author Araujo, Maria Gabriella Silva
author_facet Araujo, Maria Gabriella Silva
Santos, Regina Célia Sales
Barbosa, Lucianna Costa de Almeida
Santos, Wanderlei Barbosa Dos
Silva Junior, Edeildo Ferreira
Araújo Júnior, João Xavier de
author_role author
author2 Santos, Regina Célia Sales
Barbosa, Lucianna Costa de Almeida
Santos, Wanderlei Barbosa Dos
Silva Junior, Edeildo Ferreira
Araújo Júnior, João Xavier de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo, Maria Gabriella Silva
Santos, Regina Célia Sales
Barbosa, Lucianna Costa de Almeida
Santos, Wanderlei Barbosa Dos
Silva Junior, Edeildo Ferreira
Araújo Júnior, João Xavier de
dc.subject.por.fl_str_mv Argemone mexicana L.
Anti-bacterial agents
Cell survival
Berberine.
Argemone mexicana L.
Antibacterianos
Supervivencia celular
Berberine.
Argemone mexicana L.
Antibacterianos
Sobrevivência celular
Berberina.
topic Argemone mexicana L.
Anti-bacterial agents
Cell survival
Berberine.
Argemone mexicana L.
Antibacterianos
Supervivencia celular
Berberine.
Argemone mexicana L.
Antibacterianos
Sobrevivência celular
Berberina.
description Aim: Isolation and chemical characterization of a substance extracted from leaves of the species Argemone mexicana L. was performed. In addition, the antibacterial activity and cell viability of this substance was determined. Method: The isolation of the substance was performed by classical liquid layer chromatography, for the chemical characterization were made Infrared and Nuclear Magnetic Resonance spectroscopy. Antibacterial activity was determined by the broth microdilution method to determine the minimum inhibitory concentration of the substance. The cell viability assay was performed with the 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2Htetrazoliumbromide colorimetric method using J774 macrophage strains. Results: The substance isolated from the species Argemone mexicana L. was identified as berberine alkaloid. The ability to maintain viable cells of the substance was positive, as it not only maintained viable cells but also promoted proliferation of treated macrophages. This alkaloid demonstrated promising antibacterial activity against Gram positive and negative strains, mainly against Pseudomonas aeruginosa at a concentration of 62,5µg/ml. Conclusion: Berberine's ability to maintain and proliferate cells and inhibit bacterial growth suggests its potential for future antimicrobial treatment and in conditions that require stimulation of cell proliferation.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/19382
10.33448/rsd-v10i11.19382
url https://rsdjournal.org/index.php/rsd/article/view/19382
identifier_str_mv 10.33448/rsd-v10i11.19382
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/19382/17256
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 11; e65101119382
Research, Society and Development; Vol. 10 Núm. 11; e65101119382
Research, Society and Development; v. 10 n. 11; e65101119382
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052687568601088