Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/33419 |
Resumo: | The demand for analytical methods for drug quantification is an obstacle in the research and development of new products, due to the need to use simpler techniques for the identification and quantification of the actives. Although it is a very sensitive and selective technique for these determinations, high performance liquid chromatography (HPLC) requires expensive instrumentation and well-trained analysts, while spectrophotometry is fast, low-cost and hardly requires sample pre-treatment. Thus, this study aimed to develop a quantification method by UV-visible spectrophotometry for salicylic acid (SA) encapsulated in nanostructured lipid carriers (NLC) containing copaiba oil. The method was validated according to national and international criteria (ANVISA, ICH and FDA). The diluent was a mixture of absolute ethanol and phosphoric acid (99:1) and detection was performed at 310 nm. The interference of the matrix in relation to NLC-SA was minimal (0.37%), demonstrating the selectivity. Linearity was obtained in the range of 5.0 30.0 μg/mL (r = 0.99948) and the method proved to be accurate, with high recovery of SA in the presence of the other components (over 96% recovery), with precision at two levels: intra- and inter-days (DPR < 5%). Detection and quantification limits were 0.51 e 1.70 μg/mL, respectively. Finally, according to the Youden test criteria, the method was robust in the face of the proposed variations, obtaining effect values lower than the critical value (0.07). Therefore, the developed method is accessible, effective and valid for the quantification of SA in complex matrix lipid nanoparticles. |
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Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oilMétodo de bajo costo para la cuantificación de ácido salicílico cargado en nanopartículas lípidas que contienen aceite de copaibaMétodo de baixo custo para quantificação de ácido salicílico carregado em nanopartículas lipídicas contendo óleo de copaíbaAcne vulgarisMétodo analíticoValidación analíticaNanocargadores lipídicosEspectrofotometría UV-VisibleBioactivo vegetal.Acne vulgarisMétodo AnalíticoValidação analíticaNanocarreadores lipídicosEspectrofotometria UV-VisívelBioativo vegetal.Acne vulgarisAnalytical methodAnalytical validationLipid nanocarriersUV-Visible SpectrophotometryVegetable bioactive.The demand for analytical methods for drug quantification is an obstacle in the research and development of new products, due to the need to use simpler techniques for the identification and quantification of the actives. Although it is a very sensitive and selective technique for these determinations, high performance liquid chromatography (HPLC) requires expensive instrumentation and well-trained analysts, while spectrophotometry is fast, low-cost and hardly requires sample pre-treatment. Thus, this study aimed to develop a quantification method by UV-visible spectrophotometry for salicylic acid (SA) encapsulated in nanostructured lipid carriers (NLC) containing copaiba oil. The method was validated according to national and international criteria (ANVISA, ICH and FDA). The diluent was a mixture of absolute ethanol and phosphoric acid (99:1) and detection was performed at 310 nm. The interference of the matrix in relation to NLC-SA was minimal (0.37%), demonstrating the selectivity. Linearity was obtained in the range of 5.0 30.0 μg/mL (r = 0.99948) and the method proved to be accurate, with high recovery of SA in the presence of the other components (over 96% recovery), with precision at two levels: intra- and inter-days (DPR < 5%). Detection and quantification limits were 0.51 e 1.70 μg/mL, respectively. Finally, according to the Youden test criteria, the method was robust in the face of the proposed variations, obtaining effect values lower than the critical value (0.07). Therefore, the developed method is accessible, effective and valid for the quantification of SA in complex matrix lipid nanoparticles.La demanda de métodos analíticos para la cuantificación de fármacos es un obstáculo en la investigación y desarrollo de nuevos productos, debido a la necesidad de utilizar técnicas más sencillas para la identificación y cuantificación de los activos. Aunque es una técnica muy sensible y selectiva para estas determinaciones, la cromatografía líquida de alta resolución (HPLC) requiere instrumentación costosa y analistas bien capacitados, mientras que la espectrofotometría es rápida, de bajo costo y difícilmente requiere pretratamiento de la muestra. Por lo tanto, este estudio tuvo como objetivo desarrollar un método de cuantificación por espectrofotometría UV-visible para ácido salicílico (SA) encapsulado en portadores de lípidos nanoestructurados (NLC) que contienen aceite de copaiba. El método fue validado según criterios nacionales e internacionales (ANVISA, ICH y FDA). El diluyente fue una mezcla de etanol absoluto y ácido fosfórico (99:1) y la detección se realizó a 310 nm. La interferencia de la matriz con relación a NLC-SA fue mínima (0,37%), demostrando la selectividad. Se obtuvo linealidad en el rango de 5,0 a 30,0 μg/mL (r = 0,99948) y el método demostró ser exacto, con alta recuperación de AS en presencia de los demás componentes (más del 96% de recuperación), con precisión en dos niveles: intra e interdías (DPR < 5%). Los límites de detección y cuantificación fueron 0,51 y 1,70 μg/mL, respectivamente. Finalmente, según los criterios del índice de Youden, el método se mostró robusto ante las variaciones propuestas, obteniendo valores del efecto inferiores al valor crítico (0,07). Por tanto, el método desarrollado es accesible, eficaz y válido para la cuantificación de AS en nanopartículas lipídicas de matriz compleja.A demanda por métodos analíticos para quantificação de fármacos é um entrave na pesquisa e desenvolvimento de novos produtos, devido à necessidade de utilização de técnicas mais simples para identificação e quantificação dos ativos. Embora seja uma técnica muito sensível e seletiva para essas determinações, a cromatografia líquida de alta eficiência (HPLC) requer instrumentação cara e analistas bem treinados, enquanto a espectrofotometria é rápida, de baixo custo e dificilmente requer pré-tratamento da amostra. Assim, este estudo teve por objetivo desenvolver um método de quantificação por espectrofotometria UV-visível para ácido salicílico (SA) encapsulado em carreadores lipídicos nanoestruturados (NLC) contendo óleo de copaíba. O método foi validado segundo critérios nacionais e internacionais (ANVISA, ICH e FDA). O diluente foi uma mistura de etanol absoluto e ácido fosfórico (99:1) e a detecção foi realizada em 310 nm. A interferência da matriz em relação ao NLC-SA foi mínima (0,37%), demonstrando a seletividade. A linearidade foi obtida na faixa de 5,0 a 30,0 μg/mL (r = 0,99948) e o método se mostrou exato, com alta recuperação de SA na presença dos demais componentes (acima de 96% de recuperação), com precisão em dois níveis: intra e interdias (DPR < 5%). Os limites de detecção e quantificação foram 0,51 e 1,70 μg/mL, respectivamente. Por fim, de acordo com os critérios do teste de Youden, o método mostrou-se robusto diante das variações propostas, obtendo valores de efeito inferiores ao valor crítico (0,07). Portanto, o método desenvolvido é acessível, eficaz e válido para a quantificação de SA em nanopartículas lipídicas de matriz complexa.Research, Society and Development2022-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3341910.33448/rsd-v11i11.33419Research, Society and Development; Vol. 11 No. 11; e274111133419Research, Society and Development; Vol. 11 Núm. 11; e274111133419Research, Society and Development; v. 11 n. 11; e2741111334192525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/33419/28426Copyright (c) 2022 Bruna de Almeida Rocha; Mateus Costa Viana; João Vinícios Wirbitzki da Silveira; Guilherme Carneirohttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRocha, Bruna de Almeida Viana, Mateus CostaSilveira, João Vinícios Wirbitzki daCarneiro, Guilherme2022-09-05T13:24:46Zoai:ojs.pkp.sfu.ca:article/33419Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:49:04.487179Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil Método de bajo costo para la cuantificación de ácido salicílico cargado en nanopartículas lípidas que contienen aceite de copaiba Método de baixo custo para quantificação de ácido salicílico carregado em nanopartículas lipídicas contendo óleo de copaíba |
title |
Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil |
spellingShingle |
Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil Rocha, Bruna de Almeida Acne vulgaris Método analítico Validación analítica Nanocargadores lipídicos Espectrofotometría UV-Visible Bioactivo vegetal. Acne vulgaris Método Analítico Validação analítica Nanocarreadores lipídicos Espectrofotometria UV-Visível Bioativo vegetal. Acne vulgaris Analytical method Analytical validation Lipid nanocarriers UV-Visible Spectrophotometry Vegetable bioactive. |
title_short |
Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil |
title_full |
Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil |
title_fullStr |
Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil |
title_full_unstemmed |
Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil |
title_sort |
Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil |
author |
Rocha, Bruna de Almeida |
author_facet |
Rocha, Bruna de Almeida Viana, Mateus Costa Silveira, João Vinícios Wirbitzki da Carneiro, Guilherme |
author_role |
author |
author2 |
Viana, Mateus Costa Silveira, João Vinícios Wirbitzki da Carneiro, Guilherme |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Rocha, Bruna de Almeida Viana, Mateus Costa Silveira, João Vinícios Wirbitzki da Carneiro, Guilherme |
dc.subject.por.fl_str_mv |
Acne vulgaris Método analítico Validación analítica Nanocargadores lipídicos Espectrofotometría UV-Visible Bioactivo vegetal. Acne vulgaris Método Analítico Validação analítica Nanocarreadores lipídicos Espectrofotometria UV-Visível Bioativo vegetal. Acne vulgaris Analytical method Analytical validation Lipid nanocarriers UV-Visible Spectrophotometry Vegetable bioactive. |
topic |
Acne vulgaris Método analítico Validación analítica Nanocargadores lipídicos Espectrofotometría UV-Visible Bioactivo vegetal. Acne vulgaris Método Analítico Validação analítica Nanocarreadores lipídicos Espectrofotometria UV-Visível Bioativo vegetal. Acne vulgaris Analytical method Analytical validation Lipid nanocarriers UV-Visible Spectrophotometry Vegetable bioactive. |
description |
The demand for analytical methods for drug quantification is an obstacle in the research and development of new products, due to the need to use simpler techniques for the identification and quantification of the actives. Although it is a very sensitive and selective technique for these determinations, high performance liquid chromatography (HPLC) requires expensive instrumentation and well-trained analysts, while spectrophotometry is fast, low-cost and hardly requires sample pre-treatment. Thus, this study aimed to develop a quantification method by UV-visible spectrophotometry for salicylic acid (SA) encapsulated in nanostructured lipid carriers (NLC) containing copaiba oil. The method was validated according to national and international criteria (ANVISA, ICH and FDA). The diluent was a mixture of absolute ethanol and phosphoric acid (99:1) and detection was performed at 310 nm. The interference of the matrix in relation to NLC-SA was minimal (0.37%), demonstrating the selectivity. Linearity was obtained in the range of 5.0 30.0 μg/mL (r = 0.99948) and the method proved to be accurate, with high recovery of SA in the presence of the other components (over 96% recovery), with precision at two levels: intra- and inter-days (DPR < 5%). Detection and quantification limits were 0.51 e 1.70 μg/mL, respectively. Finally, according to the Youden test criteria, the method was robust in the face of the proposed variations, obtaining effect values lower than the critical value (0.07). Therefore, the developed method is accessible, effective and valid for the quantification of SA in complex matrix lipid nanoparticles. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/33419 10.33448/rsd-v11i11.33419 |
url |
https://rsdjournal.org/index.php/rsd/article/view/33419 |
identifier_str_mv |
10.33448/rsd-v11i11.33419 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/33419/28426 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 11; e274111133419 Research, Society and Development; Vol. 11 Núm. 11; e274111133419 Research, Society and Development; v. 11 n. 11; e274111133419 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052720771760128 |